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I tried to use bustools linker today to analyze combinatorial barcoding data and I encountered some really weird behavior. It can be demonstrated using the following code (current binary release, output.bus file from test/ directory, tested on Ubuntu 18.04):
Hi,
I tried to use bustools linker today to analyze combinatorial barcoding data and I encountered some really weird behavior. It can be demonstrated using the following code (current binary release, output.bus file from test/ directory, tested on Ubuntu 18.04):
./bustools text -p output.bus | head
./bustools linker -p -s 0 -e 1 output.bus | ./bustools text -p - | head
The command is supposed to remove the first base of the barcode; instead it introduces multiple changes to the barcode sequence
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