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Question regarding GFF file generation #127

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fischer-hub opened this issue Jul 15, 2024 · 2 comments
Open

Question regarding GFF file generation #127

fischer-hub opened this issue Jul 15, 2024 · 2 comments
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@fischer-hub
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fischer-hub commented Jul 15, 2024

Hey, thanks for this nice pipeline!

I'm running the pipeline with the --list argument providing a sample sheet to it to run on ~100 samples. But for all the viral sequences that are identified by the pipeline I only get one GFF file in the output directory of the first sample from the sample sheet. I think this is cause by this line of code and I was just wondering what the reasoning behind taking only runnning the GFF script on the first contig file as a reference sequence is.

I get that in the case of running the pipeline on a single sample this doesn't make a difference, but when dealing with multiple samples wouldn't it make sense to generate a GFF file for every sample where viral sequences have been found? Just wondering if I'm missing something here!

Thanks in advance!

@mberacochea
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Hey @fischer-hub

That looks like a bug to me, we usually run the pipeline one assembly at the time. So I think it's just an unhappy coincidence. I'll change it and will test the pipeline.

Cheers

@mberacochea mberacochea self-assigned this Jul 20, 2024
@mberacochea mberacochea added the bug Something isn't working label Jul 20, 2024
@fischer-hub
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Thanks again!

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