From 84720ac861a046d264615c13da702bc539894a25 Mon Sep 17 00:00:00 2001 From: Sander Willems Date: Mon, 8 Jul 2024 11:10:22 +0200 Subject: [PATCH] DOCS: updated docs --- README.md | 17 ++++++++++------- 1 file changed, 10 insertions(+), 7 deletions(-) diff --git a/README.md b/README.md index 98ed090..284c54a 100644 --- a/README.md +++ b/README.md @@ -9,19 +9,22 @@ This package is intended to convert a clusters.hdf file with alphasynchro data i * alphasynchro.zip * Steps - * Unpack timspeak.zip and alphasynchro.zip + * Download timspeak: `git clone https://github.com/MannLabs/timspeak.git` + * Download alphasynchro: `git clone https://github.com/MannLabs/alphasynchro.git` * Create conda env: `conda create -n synchro python=3.10 -y` * Activate: `conda activate synchro` - * Install msconvert (mgf->mzml): `conda install -c bioconda proteowizard -y` * Install Sage: `conda install -c bioconda -c conda-forge sage-proteomics -y` * Install timspeak and alphasynchro: `pip install -e ./timspeak -e ./alphasynchro` ## Usage * Required - * timspeak.config - * sage.config - * sample.fasta + * Config folder (e.g. [example](example/)) containing: + * timspeak.config + * sage.config + * species.fasta * raw_data.d - -See the [command-line input](example/cmd.txt) in the [example folder](example/). +* Usage + * Copy-paste [examplary command-line input](example/cmd.sh) in the [example folder](example/) and modify. + * Note: if working with regular dia-PASEF rather than synchro-PASEF, use line 15 instead of 14 (add the `--diapasef`, but furtherwise identical). + * Run `./example/cmd.sh`