diff --git a/directlfq/__init__.py b/directlfq/__init__.py
index a3d7827..f962452 100644
--- a/directlfq/__init__.py
+++ b/directlfq/__init__.py
@@ -2,7 +2,7 @@
 
 
 __project__ = "directlfq"
-__version__ = "0.2.18"
+__version__ = "0.2.19"
 __license__ = "Apache"
 __description__ = "An open-source Python package of the AlphaPept ecosystem"
 __author__ = "Mann Labs"
diff --git a/directlfq/config.py b/directlfq/config.py
index e5037d2..b9d749f 100644
--- a/directlfq/config.py
+++ b/directlfq/config.py
@@ -1,10 +1,11 @@
 import logging
+import pandas as pd
 
 
 def setup_logging():
     logging.basicConfig(level=logging.INFO, 
                         format='%(asctime)s - %(name)s - %(levelname)s - %(message)s')
-
+setup_logging()
 ##########################
 LOG_PROCESSED_PROTEINS = True
 
@@ -30,3 +31,27 @@ def set_compile_normalized_ion_table(compile_normalized_ion_table = True):
     global COMPILE_NORMALIZED_ION_TABLE
     COMPILE_NORMALIZED_ION_TABLE = compile_normalized_ion_table
 
+##########################
+COPY_NUMPY_ARRAYS_DERIVED_FROM_PANDAS = False
+
+def check_wether_to_copy_numpy_arrays_derived_from_pandas():
+    global COPY_NUMPY_ARRAYS_DERIVED_FROM_PANDAS
+    try:
+        _manipulate_numpy_array_without_copy()
+        COPY_NUMPY_ARRAYS_DERIVED_FROM_PANDAS = False
+    except:
+        logging.info('Some numpy arrays derived from pandas will be copied.')
+        COPY_NUMPY_ARRAYS_DERIVED_FROM_PANDAS = True
+
+def _manipulate_numpy_array_without_copy():
+    
+    protein_profile_df = pd.DataFrame({
+    'ProteinA': [10, 20, 30, 40],
+    'ProteinB': [15, 25, 35, 45],
+    'ProteinC': [20, 30, 40, 50]
+    }, index=['Sample1', 'Sample2', 'Sample3', 'Sample4'])
+    
+    protein_profile_df = protein_profile_df.iloc[1:3]
+    protein_profile_numpy = protein_profile_df.to_numpy(copy=False)
+
+    protein_profile_numpy[0] = protein_profile_numpy[0] +2
diff --git a/directlfq/lfq_manager.py b/directlfq/lfq_manager.py
index c154788..7c30fae 100644
--- a/directlfq/lfq_manager.py
+++ b/directlfq/lfq_manager.py
@@ -39,6 +39,7 @@ def run_lfq(input_file,  columns_to_add = [], selected_proteins_file :str = None
     config.set_global_protein_and_ion_id(protein_id=protein_id, quant_id=quant_id)
     config.set_log_processed_proteins(log_processed_proteins=log_processed_proteins)
     config.set_compile_normalized_ion_table(compile_normalized_ion_table= compile_normalized_ion_table)
+    config.check_wether_to_copy_numpy_arrays_derived_from_pandas()
 
     LOGGER.info("Starting directLFQ analysis.")
     input_file = prepare_input_filename(input_file)
diff --git a/directlfq/normalization.py b/directlfq/normalization.py
index e957c3e..50423d4 100644
--- a/directlfq/normalization.py
+++ b/directlfq/normalization.py
@@ -225,7 +225,7 @@ def normalize_dataframe_between_samples(ion_dataframe):
     return df_c_normed
 
 def normalize_ion_profiles(protein_profile_df):
-    protein_profile_numpy = protein_profile_df.to_numpy()
+    protein_profile_numpy = protein_profile_df.to_numpy(copy = config.COPY_NUMPY_ARRAYS_DERIVED_FROM_PANDAS)
     sample2shift = get_normfacts(protein_profile_numpy)
     df_normed = pd.DataFrame(apply_sampleshifts(protein_profile_numpy, sample2shift), index = protein_profile_df.index, columns = protein_profile_df.columns)
     return df_normed
@@ -344,7 +344,7 @@ def _normalization_function(self, ion_dataframe):
             ion_dataframe_selected = ion_dataframe
             sample2shift = get_normfacts(drop_nas_if_possible(ion_dataframe_selected).to_numpy())
 
-        df_c_normed = pd.DataFrame(apply_sampleshifts(ion_dataframe.to_numpy(), sample2shift), index = ion_dataframe.index, columns = ion_dataframe.columns)
+        df_c_normed = pd.DataFrame(apply_sampleshifts(ion_dataframe.to_numpy(copy = config.COPY_NUMPY_ARRAYS_DERIVED_FROM_PANDAS), sample2shift), index = ion_dataframe.index, columns = ion_dataframe.columns)
         return df_c_normed
     
     def _create_reference_sample(self):
diff --git a/directlfq/utils.py b/directlfq/utils.py
index 857baa9..0229b07 100644
--- a/directlfq/utils.py
+++ b/directlfq/utils.py
@@ -130,22 +130,8 @@ def get_nonna_array(array_w_nas):
         res.append(np.array(sub_res))
     return np.array(res)
 
-# %% ../nbdev_nbs/04_utils.ipynb 14
-import numpy as np
-def get_non_nas_from_pd_df(df):
-    return {
-        pep_name: sub_vals[~np.isnan(sub_vals)] for pep_name, sub_vals in
-        zip( df.index.values, df.values)
-    }
 
 # %% ../nbdev_nbs/04_utils.ipynb 15
-import numpy as np
-def get_ionints_from_pd_df(df):
-    return {
-        pep_name: sub_vals for pep_name, sub_vals in
-        zip( df.index.values, df.values)
-    }
-
 # %% ../nbdev_nbs/04_utils.ipynb 16
 def invert_dictionary(my_map):
     inv_map = {}
@@ -313,17 +299,14 @@ def show_diff(df1, df2):
     return df1.merge(df2, indicator=True, how='outer').loc[lambda x : x['_merge']!='both']
 
 
-# %% ../nbdev_nbs/04_utils.ipynb 23
 def write_chunk_to_file(chunk, filepath ,write_header):
     """write chunk of pandas dataframe to a file"""
     chunk.to_csv(filepath, header=write_header, mode='a', sep = "\t", index = None)
 
-# %% ../nbdev_nbs/04_utils.ipynb 24
 def index_and_log_transform_input_df(data_df):
     data_df = data_df.set_index([config.PROTEIN_ID, config.QUANT_ID])
     return np.log2(data_df.replace(0, np.nan))
 
-# %% ../nbdev_nbs/04_utils.ipynb 25
 def remove_allnan_rows_input_df(data_df):
     return data_df.dropna(axis = 0, how = 'all')
 
diff --git a/misc/bumpversion.cfg b/misc/bumpversion.cfg
index e31e27c..8910cd5 100644
--- a/misc/bumpversion.cfg
+++ b/misc/bumpversion.cfg
@@ -1,5 +1,5 @@
 [bumpversion]
-current_version = 0.2.18
+current_version = 0.2.19
 commit = True
 tag = False
 parse = (?P<major>\d+)\.(?P<minor>\d+)\.(?P<patch>\d+)(\-(?P<release>[a-z]+)(?P<build>\d+))?
diff --git a/release/one_click_linux_gui/control b/release/one_click_linux_gui/control
index 796c7fc..c0c1804 100644
--- a/release/one_click_linux_gui/control
+++ b/release/one_click_linux_gui/control
@@ -1,5 +1,5 @@
 Package: directlfq
-Version: 0.2.18
+Version: 0.2.19
 Architecture: all
 Maintainer: Mann Labs <opensource@alphapept.com>
 Description: directlfq
diff --git a/release/one_click_linux_gui/create_installer_linux.sh b/release/one_click_linux_gui/create_installer_linux.sh
index b33d63b..6b7e10d 100644
--- a/release/one_click_linux_gui/create_installer_linux.sh
+++ b/release/one_click_linux_gui/create_installer_linux.sh
@@ -17,7 +17,7 @@ python setup.py sdist bdist_wheel
 # Setting up the local package
 cd release/one_click_linux_gui
 # Make sure you include the required extra packages and always use the stable or very-stable options!
-pip install "../../dist/directlfq-0.2.18-py3-none-any.whl[stable, gui]"
+pip install "../../dist/directlfq-0.2.19-py3-none-any.whl[stable, gui]"
 
 # Creating the stand-alone pyinstaller folder
 pip install pyinstaller==4.10
diff --git a/release/one_click_macos_gui/Info.plist b/release/one_click_macos_gui/Info.plist
index 7cb5a40..9f96f3a 100644
--- a/release/one_click_macos_gui/Info.plist
+++ b/release/one_click_macos_gui/Info.plist
@@ -9,9 +9,9 @@
 	<key>CFBundleIconFile</key>
 	<string>alpha_logo.icns</string>
 	<key>CFBundleIdentifier</key>
-	<string>directlfq.0.2.18</string>
+	<string>directlfq.0.2.19</string>
 	<key>CFBundleShortVersionString</key>
-	<string>0.2.18</string>
+	<string>0.2.19</string>
 	<key>CFBundleInfoDictionaryVersion</key>
 	<string>6.0</string>
 	<key>CFBundleName</key>
diff --git a/release/one_click_macos_gui/create_installer_macos.sh b/release/one_click_macos_gui/create_installer_macos.sh
index 6e5f754..f4106a1 100755
--- a/release/one_click_macos_gui/create_installer_macos.sh
+++ b/release/one_click_macos_gui/create_installer_macos.sh
@@ -20,7 +20,7 @@ python setup.py sdist bdist_wheel
 
 # Setting up the local package
 cd release/one_click_macos_gui
-pip install "../../dist/directlfq-0.2.18-py3-none-any.whl[stable, gui]"
+pip install "../../dist/directlfq-0.2.19-py3-none-any.whl[stable, gui]"
 
 # Creating the stand-alone pyinstaller folder
 pip install pyinstaller==4.10
@@ -40,5 +40,5 @@ cp ../../LICENSE Resources/LICENSE
 cp ../logos/alpha_logo.png Resources/alpha_logo.png
 chmod 777 scripts/*
 
-pkgbuild --root dist/directlfq --identifier de.mpg.biochem.directlfq.app --version 0.2.18 --install-location /Applications/directlfq.app --scripts scripts directlfq.pkg
+pkgbuild --root dist/directlfq --identifier de.mpg.biochem.directlfq.app --version 0.2.19 --install-location /Applications/directlfq.app --scripts scripts directlfq.pkg
 productbuild --distribution distribution.xml --resources Resources --package-path directlfq.pkg dist/directlfq_gui_installer_macos.pkg
diff --git a/release/one_click_macos_gui/distribution.xml b/release/one_click_macos_gui/distribution.xml
index fb131b0..2280b85 100644
--- a/release/one_click_macos_gui/distribution.xml
+++ b/release/one_click_macos_gui/distribution.xml
@@ -1,6 +1,6 @@
 <?xml version="1.0" encoding="utf-8" standalone="no"?>
 <installer-script minSpecVersion="1.000000">
-    <title>directlfq 0.2.18</title>
+    <title>directlfq 0.2.19</title>
     <background mime-type="image/png" file="alpha_logo.png" scaling="proportional"/>
     <welcome file="welcome.html" mime-type="text/html" />
     <conclusion file="conclusion.html" mime-type="text/html" />
diff --git a/release/one_click_windows_gui/create_installer_windows.sh b/release/one_click_windows_gui/create_installer_windows.sh
index 0108762..774d10d 100644
--- a/release/one_click_windows_gui/create_installer_windows.sh
+++ b/release/one_click_windows_gui/create_installer_windows.sh
@@ -17,7 +17,7 @@ python setup.py sdist bdist_wheel
 # Setting up the local package
 cd release/one_click_windows_gui
 # Make sure you include the required extra packages and always use the stable or very-stable options!
-pip install "../../dist/directlfq-0.2.18-py3-none-any.whl[stable, gui]"
+pip install "../../dist/directlfq-0.2.19-py3-none-any.whl[stable, gui]"
 
 # Creating the stand-alone pyinstaller folder
 pip install pyinstaller==4.10
diff --git a/release/one_click_windows_gui/directlfq_innoinstaller.iss b/release/one_click_windows_gui/directlfq_innoinstaller.iss
index 034e297..19d876e 100644
--- a/release/one_click_windows_gui/directlfq_innoinstaller.iss
+++ b/release/one_click_windows_gui/directlfq_innoinstaller.iss
@@ -2,7 +2,7 @@
 ; SEE THE DOCUMENTATION FOR DETAILS ON CREATING INNO SETUP SCRIPT FILES!
 
 #define MyAppName "directlfq"
-#define MyAppVersion "0.2.18"
+#define MyAppVersion "0.2.19"
 #define MyAppPublisher "Max Planck Institute of Biochemistry and the University of Copenhagen, Mann Labs"
 #define MyAppURL "https://github.com/MannLabs/directlfq"
 #define MyAppExeName "directlfq_gui.exe"
diff --git a/settings.ini b/settings.ini
index 08ee04c..60d25af 100644
--- a/settings.ini
+++ b/settings.ini
@@ -13,7 +13,7 @@ author = Constantin Ammar
 author_email = constantin.ammar@gmail.com
 copyright = fast.ai
 branch = master
-version = 0.2.18
+version = 0.2.19
 min_python = 3.6
 audience = Developers
 language = English
diff --git a/tests/quicktests/run_pipeline_w_different_input_formats.ipynb b/tests/quicktests/run_pipeline_w_different_input_formats.ipynb
index ec700cd..9a6d1f1 100644
--- a/tests/quicktests/run_pipeline_w_different_input_formats.ipynb
+++ b/tests/quicktests/run_pipeline_w_different_input_formats.ipynb
@@ -2,7 +2,7 @@
  "cells": [
   {
    "cell_type": "code",
-   "execution_count": 1,
+   "execution_count": null,
    "metadata": {},
    "outputs": [],
    "source": [
@@ -19,61 +19,9 @@
   },
   {
    "cell_type": "code",
-   "execution_count": 3,
+   "execution_count": null,
    "metadata": {},
-   "outputs": [
-    {
-     "name": "stderr",
-     "output_type": "stream",
-     "text": [
-      "2024-02-21 15:15:50,514 - directlfq.lfq_manager - INFO - Starting directLFQ analysis.\n",
-      "2024-02-21 15:15:50,664 - directlfq.utils - INFO - using input type diann_precursors\n",
-      "2024-02-21 15:15:50,722 - directlfq.lfq_manager - INFO - Performing sample normalization.\n",
-      "2024-02-21 15:15:50,724 - directlfq.normalization - INFO - to few values for normalization without missing values. Including missing values\n",
-      "2024-02-21 15:15:50,727 - directlfq.lfq_manager - INFO - Estimating lfq intensities.\n",
-      "2024-02-21 15:15:50,727 - directlfq.protein_intensity_estimation - INFO - 46 lfq-groups total\n",
-      "2024-02-21 15:15:50,744 - directlfq.protein_intensity_estimation - INFO - lfq-object 0\n",
-      "2024-02-21 15:15:51,341 - directlfq.lfq_manager - INFO - Writing results files.\n",
-      "2024-02-21 15:15:51,355 - directlfq.lfq_manager - INFO - Analysis finished!\n",
-      "2024-02-21 15:15:51,355 - directlfq.lfq_manager - INFO - Starting directLFQ analysis.\n",
-      "2024-02-21 15:15:51,473 - directlfq.utils - INFO - using input type diann_peptide_based_on_precursor_ms1_and_ms2\n",
-      "2024-02-21 15:15:51,618 - directlfq.lfq_manager - INFO - Performing sample normalization.\n",
-      "2024-02-21 15:15:51,623 - directlfq.lfq_manager - INFO - Estimating lfq intensities.\n",
-      "2024-02-21 15:15:51,624 - directlfq.protein_intensity_estimation - INFO - 840 lfq-groups total\n",
-      "2024-02-21 15:15:51,936 - directlfq.protein_intensity_estimation - INFO - using 10 processes\n",
-      "2024-02-21 15:15:51,959 - directlfq.protein_intensity_estimation - INFO - lfq-object 0\n",
-      "2024-02-21 15:15:52,016 - directlfq.protein_intensity_estimation - INFO - lfq-object 100\n",
-      "2024-02-21 15:15:52,073 - directlfq.protein_intensity_estimation - INFO - lfq-object 200\n",
-      "2024-02-21 15:15:52,125 - directlfq.protein_intensity_estimation - INFO - lfq-object 300\n",
-      "2024-02-21 15:15:52,180 - directlfq.protein_intensity_estimation - INFO - lfq-object 400\n",
-      "2024-02-21 15:15:52,244 - directlfq.protein_intensity_estimation - INFO - lfq-object 500\n",
-      "2024-02-21 15:15:52,299 - directlfq.protein_intensity_estimation - INFO - lfq-object 600\n",
-      "2024-02-21 15:15:52,354 - directlfq.protein_intensity_estimation - INFO - lfq-object 700\n",
-      "2024-02-21 15:15:52,408 - directlfq.protein_intensity_estimation - INFO - lfq-object 800\n",
-      "2024-02-21 15:15:52,609 - directlfq.lfq_manager - INFO - Writing results files.\n",
-      "2024-02-21 15:15:52,639 - directlfq.lfq_manager - INFO - Analysis finished!\n",
-      "2024-02-21 15:15:52,640 - directlfq.lfq_manager - INFO - Starting directLFQ analysis.\n",
-      "2024-02-21 15:15:52,765 - directlfq.utils - INFO - using input type diann_precursor_ms1_and_ms2\n",
-      "2024-02-21 15:15:52,878 - directlfq.lfq_manager - INFO - Performing sample normalization.\n",
-      "2024-02-21 15:15:52,883 - directlfq.lfq_manager - INFO - Estimating lfq intensities.\n",
-      "2024-02-21 15:15:52,884 - directlfq.protein_intensity_estimation - INFO - 46 lfq-groups total\n",
-      "2024-02-21 15:15:52,952 - directlfq.protein_intensity_estimation - INFO - using 10 processes\n",
-      "2024-02-21 15:15:52,960 - directlfq.protein_intensity_estimation - INFO - lfq-object 0\n",
-      "2024-02-21 15:15:53,274 - directlfq.lfq_manager - INFO - Writing results files.\n",
-      "2024-02-21 15:15:53,295 - directlfq.lfq_manager - INFO - Analysis finished!\n",
-      "2024-02-21 15:15:53,296 - directlfq.lfq_manager - INFO - Starting directLFQ analysis.\n",
-      "2024-02-21 15:15:53,371 - directlfq.utils - INFO - using input type diann_precursor_ms1_and_ms2\n",
-      "2024-02-21 15:15:53,505 - directlfq.lfq_manager - INFO - Performing sample normalization.\n",
-      "2024-02-21 15:15:53,506 - root - INFO - Normalizing only selected proteins\n",
-      "2024-02-21 15:15:53,516 - directlfq.lfq_manager - INFO - Estimating lfq intensities.\n",
-      "2024-02-21 15:15:53,517 - directlfq.protein_intensity_estimation - INFO - 46 lfq-groups total\n",
-      "2024-02-21 15:15:53,630 - directlfq.protein_intensity_estimation - INFO - using 10 processes\n",
-      "2024-02-21 15:15:53,644 - directlfq.protein_intensity_estimation - INFO - lfq-object 0\n",
-      "2024-02-21 15:15:53,997 - directlfq.lfq_manager - INFO - Writing results files.\n",
-      "2024-02-21 15:15:54,022 - directlfq.lfq_manager - INFO - Analysis finished!\n"
-     ]
-    }
-   ],
+   "outputs": [],
    "source": [
     "# run diann\n",
     "import directlfq.lfq_manager as lfq_manager\n",
@@ -90,25 +38,9 @@
   },
   {
    "cell_type": "code",
-   "execution_count": 4,
+   "execution_count": null,
    "metadata": {},
-   "outputs": [
-    {
-     "name": "stderr",
-     "output_type": "stream",
-     "text": [
-      "2024-02-21 15:15:54,029 - directlfq.lfq_manager - INFO - Starting directLFQ analysis.\n",
-      "2024-02-21 15:15:54,191 - directlfq.utils - INFO - using input type maxquant_peptides_leading_razor_protein\n",
-      "2024-02-21 15:15:54,237 - directlfq.lfq_manager - INFO - Performing sample normalization.\n",
-      "2024-02-21 15:15:54,239 - directlfq.normalization - INFO - to few values for normalization without missing values. Including missing values\n",
-      "2024-02-21 15:15:54,245 - directlfq.lfq_manager - INFO - Estimating lfq intensities.\n",
-      "2024-02-21 15:15:54,245 - directlfq.protein_intensity_estimation - INFO - 49 lfq-groups total\n",
-      "2024-02-21 15:15:54,263 - directlfq.protein_intensity_estimation - INFO - lfq-object 0\n",
-      "2024-02-21 15:15:54,816 - directlfq.lfq_manager - INFO - Writing results files.\n",
-      "2024-02-21 15:15:54,831 - directlfq.lfq_manager - INFO - Analysis finished!\n"
-     ]
-    }
-   ],
+   "outputs": [],
    "source": [
     "#run mq peptides\n",
     "\n",
@@ -124,35 +56,9 @@
   },
   {
    "cell_type": "code",
-   "execution_count": 5,
+   "execution_count": null,
    "metadata": {},
-   "outputs": [
-    {
-     "name": "stderr",
-     "output_type": "stream",
-     "text": [
-      "2024-02-21 15:15:54,837 - directlfq.lfq_manager - INFO - Starting directLFQ analysis.\n",
-      "2024-02-21 15:15:54,987 - directlfq.utils - INFO - using input type maxquant_evidence_leading_razor_protein\n",
-      "2024-02-21 15:15:55,088 - directlfq.lfq_manager - INFO - Performing sample normalization.\n",
-      "2024-02-21 15:15:55,090 - directlfq.normalization - INFO - to few values for normalization without missing values. Including missing values\n",
-      "2024-02-21 15:15:55,096 - directlfq.lfq_manager - INFO - Estimating lfq intensities.\n",
-      "2024-02-21 15:15:55,096 - directlfq.protein_intensity_estimation - INFO - 50 lfq-groups total\n",
-      "2024-02-21 15:15:55,166 - directlfq.protein_intensity_estimation - INFO - using 10 processes\n",
-      "2024-02-21 15:15:55,173 - directlfq.protein_intensity_estimation - INFO - lfq-object 0\n",
-      "2024-02-21 15:15:55,396 - directlfq.lfq_manager - INFO - Writing results files.\n",
-      "2024-02-21 15:15:55,413 - directlfq.lfq_manager - INFO - Analysis finished!\n",
-      "2024-02-21 15:15:55,413 - directlfq.lfq_manager - INFO - Starting directLFQ analysis.\n",
-      "2024-02-21 15:15:55,563 - directlfq.utils - INFO - using input type maxquant_evidence_leading_razor_protein\n",
-      "2024-02-21 15:15:55,663 - directlfq.lfq_manager - INFO - Performing sample normalization.\n",
-      "2024-02-21 15:15:55,664 - root - INFO - Normalizing only selected proteins\n",
-      "2024-02-21 15:15:55,671 - directlfq.lfq_manager - INFO - Estimating lfq intensities.\n",
-      "2024-02-21 15:15:55,671 - directlfq.protein_intensity_estimation - INFO - 50 lfq-groups total\n",
-      "2024-02-21 15:15:55,688 - directlfq.protein_intensity_estimation - INFO - lfq-object 0\n",
-      "2024-02-21 15:15:56,371 - directlfq.lfq_manager - INFO - Writing results files.\n",
-      "2024-02-21 15:15:56,388 - directlfq.lfq_manager - INFO - Analysis finished!\n"
-     ]
-    }
-   ],
+   "outputs": [],
    "source": [
     "#run mq evidence\n",
     "\n",
@@ -168,34 +74,9 @@
   },
   {
    "cell_type": "code",
-   "execution_count": 6,
+   "execution_count": null,
    "metadata": {},
-   "outputs": [
-    {
-     "name": "stderr",
-     "output_type": "stream",
-     "text": [
-      "2024-02-21 15:15:56,395 - directlfq.lfq_manager - INFO - Starting directLFQ analysis.\n",
-      "2024-02-21 15:15:56,548 - directlfq.utils - INFO - using input type spectronaut_fragion_isotopes\n",
-      "2024-02-21 15:15:56,968 - directlfq.lfq_manager - INFO - Performing sample normalization.\n",
-      "2024-02-21 15:15:56,969 - root - INFO - Normalizing only selected proteins\n",
-      "2024-02-21 15:15:56,981 - directlfq.lfq_manager - INFO - Estimating lfq intensities.\n",
-      "2024-02-21 15:15:56,983 - directlfq.protein_intensity_estimation - INFO - 50 lfq-groups total\n",
-      "2024-02-21 15:15:57,004 - directlfq.protein_intensity_estimation - INFO - lfq-object 0\n",
-      "2024-02-21 15:15:58,512 - directlfq.lfq_manager - INFO - Writing results files.\n",
-      "2024-02-21 15:15:58,546 - directlfq.lfq_manager - INFO - Analysis finished!\n",
-      "2024-02-21 15:15:58,547 - directlfq.lfq_manager - INFO - Starting directLFQ analysis.\n",
-      "2024-02-21 15:15:58,697 - directlfq.utils - INFO - using input type spectronaut_fragion_isotopes\n",
-      "2024-02-21 15:15:59,120 - directlfq.lfq_manager - INFO - Performing sample normalization.\n",
-      "2024-02-21 15:15:59,130 - directlfq.lfq_manager - INFO - Estimating lfq intensities.\n",
-      "2024-02-21 15:15:59,131 - directlfq.protein_intensity_estimation - INFO - 50 lfq-groups total\n",
-      "2024-02-21 15:15:59,211 - directlfq.protein_intensity_estimation - INFO - using 10 processes\n",
-      "2024-02-21 15:15:59,219 - directlfq.protein_intensity_estimation - INFO - lfq-object 0\n",
-      "2024-02-21 15:15:59,735 - directlfq.lfq_manager - INFO - Writing results files.\n",
-      "2024-02-21 15:15:59,764 - directlfq.lfq_manager - INFO - Analysis finished!\n"
-     ]
-    }
-   ],
+   "outputs": [],
    "source": [
     "# run spectronaut\n",
     "import directlfq.lfq_manager as lfq_manager\n",
diff --git a/tests/ratio_tests/compare_diapasef_diann.ipynb b/tests/ratio_tests/compare_diapasef_diann.ipynb
index 4fbf7c1..021b3f5 100644
--- a/tests/ratio_tests/compare_diapasef_diann.ipynb
+++ b/tests/ratio_tests/compare_diapasef_diann.ipynb
@@ -24,7 +24,7 @@
   },
   {
    "cell_type": "code",
-   "execution_count": 4,
+   "execution_count": 2,
    "metadata": {},
    "outputs": [],
    "source": [
@@ -58,134 +58,134 @@
      "name": "stderr",
      "output_type": "stream",
      "text": [
-      "2024-02-21 14:38:20,749 - directlfq.lfq_manager - INFO - Starting directLFQ analysis.\n",
-      "2024-02-21 14:38:20,834 - directlfq.utils - INFO - using input type diann_precursor_ms1_and_ms2\n",
-      "2024-02-21 14:38:37,192 - directlfq.lfq_manager - INFO - Performing sample normalization.\n",
-      "2024-02-21 14:38:38,868 - directlfq.lfq_manager - INFO - Estimating lfq intensities.\n",
-      "2024-02-21 14:38:38,894 - directlfq.protein_intensity_estimation - INFO - 11983 lfq-groups total\n",
-      "2024-02-21 14:38:42,844 - directlfq.protein_intensity_estimation - INFO - using 10 processes\n",
-      "2024-02-21 14:38:43,287 - directlfq.protein_intensity_estimation - INFO - lfq-object 0\n",
-      "2024-02-21 14:38:43,466 - directlfq.protein_intensity_estimation - INFO - lfq-object 300\n",
-      "2024-02-21 14:38:43,642 - directlfq.protein_intensity_estimation - INFO - lfq-object 600\n",
-      "2024-02-21 14:38:43,941 - directlfq.protein_intensity_estimation - INFO - lfq-object 900\n",
-      "2024-02-21 14:38:44,000 - directlfq.protein_intensity_estimation - INFO - lfq-object 1200\n",
-      "2024-02-21 14:38:44,208 - directlfq.protein_intensity_estimation - INFO - lfq-object 1500\n",
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-      "2024-02-21 14:38:45,352 - directlfq.protein_intensity_estimation - INFO - lfq-object 2700\n",
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-      "2024-02-21 14:39:03,730 - directlfq.protein_intensity_estimation - INFO - lfq-object 11200\n",
-      "2024-02-21 14:39:03,953 - directlfq.protein_intensity_estimation - INFO - lfq-object 11500\n",
-      "2024-02-21 14:39:04,227 - directlfq.protein_intensity_estimation - INFO - lfq-object 10400\n",
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-      "2024-02-21 14:39:04,398 - directlfq.protein_intensity_estimation - INFO - lfq-object 10700\n",
-      "2024-02-21 14:39:04,924 - directlfq.protein_intensity_estimation - INFO - lfq-object 11000\n",
-      "2024-02-21 14:39:05,136 - directlfq.protein_intensity_estimation - INFO - lfq-object 11300\n",
-      "2024-02-21 14:39:05,587 - directlfq.protein_intensity_estimation - INFO - lfq-object 11600\n",
-      "2024-02-21 14:39:05,756 - directlfq.protein_intensity_estimation - INFO - lfq-object 11900\n",
-      "2024-02-21 14:39:12,415 - directlfq.lfq_manager - INFO - Writing results files.\n",
-      "2024-02-21 14:39:15,739 - directlfq.lfq_manager - INFO - Analysis finished!\n"
+      "2024-04-09 11:34:35,415 - directlfq.lfq_manager - INFO - Starting directLFQ analysis.\n",
+      "2024-04-09 11:34:35,485 - directlfq.utils - INFO - using input type diann_precursor_ms1_and_ms2\n",
+      "2024-04-09 11:34:48,967 - directlfq.lfq_manager - INFO - Performing sample normalization.\n",
+      "2024-04-09 11:34:50,270 - directlfq.lfq_manager - INFO - Estimating lfq intensities.\n",
+      "2024-04-09 11:34:50,291 - directlfq.protein_intensity_estimation - INFO - 11983 lfq-groups total\n",
+      "2024-04-09 11:34:53,494 - directlfq.protein_intensity_estimation - INFO - using 10 processes\n",
+      "2024-04-09 11:34:53,851 - directlfq.protein_intensity_estimation - INFO - lfq-object 0\n",
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+      "2024-04-09 11:35:19,642 - directlfq.lfq_manager - INFO - Writing results files.\n",
+      "2024-04-09 11:35:22,315 - directlfq.lfq_manager - INFO - Analysis finished!\n"
      ]
     }
    ],
@@ -197,7 +197,7 @@
   },
   {
    "cell_type": "code",
-   "execution_count": 5,
+   "execution_count": 4,
    "metadata": {},
    "outputs": [],
    "source": [
@@ -208,7 +208,7 @@
   },
   {
    "cell_type": "code",
-   "execution_count": 6,
+   "execution_count": 5,
    "metadata": {},
    "outputs": [],
    "source": [
@@ -221,7 +221,7 @@
   },
   {
    "cell_type": "code",
-   "execution_count": 7,
+   "execution_count": 6,
    "metadata": {},
    "outputs": [
     {
@@ -272,7 +272,7 @@
   },
   {
    "cell_type": "code",
-   "execution_count": 8,
+   "execution_count": 7,
    "metadata": {},
    "outputs": [
     {
@@ -954,7 +954,7 @@
   },
   {
    "cell_type": "code",
-   "execution_count": 9,
+   "execution_count": 8,
    "metadata": {},
    "outputs": [],
    "source": [
@@ -965,7 +965,7 @@
   },
   {
    "cell_type": "code",
-   "execution_count": 10,
+   "execution_count": 9,
    "metadata": {},
    "outputs": [],
    "source": [
@@ -980,7 +980,7 @@
   },
   {
    "cell_type": "code",
-   "execution_count": 11,
+   "execution_count": 10,
    "metadata": {},
    "outputs": [],
    "source": [
@@ -1059,7 +1059,7 @@
   },
   {
    "cell_type": "code",
-   "execution_count": 12,
+   "execution_count": 11,
    "metadata": {},
    "outputs": [
     {
@@ -1448,7 +1448,7 @@
   },
   {
    "cell_type": "code",
-   "execution_count": 13,
+   "execution_count": 12,
    "metadata": {},
    "outputs": [
     {
@@ -1469,6 +1469,11 @@
       "YEAST num:3097 median_FC:1.4 STD:0.33\n",
       "\n",
       "HUMAN num:8571 median_FC:-0.025 STD:0.11\n",
+      "\n",
+      "directLFQ (fragions)\n",
+      "YEAST num:3097 median_FC:1.4 STD:0.32\n",
+      "\n",
+      "HUMAN num:8571 median_FC:-0.025 STD:0.1\n",
       "\n"
      ]
     },
@@ -1481,6 +1486,8 @@
       "/Users/constantin/opt/anaconda3/envs/directlfq/lib/python3.8/site-packages/pandas/core/arraylike.py:402: RuntimeWarning: divide by zero encountered in log2\n",
       "  result = getattr(ufunc, method)(*inputs, **kwargs)\n",
       "/Users/constantin/opt/anaconda3/envs/directlfq/lib/python3.8/site-packages/pandas/core/arraylike.py:402: RuntimeWarning: divide by zero encountered in log2\n",
+      "  result = getattr(ufunc, method)(*inputs, **kwargs)\n",
+      "/Users/constantin/opt/anaconda3/envs/directlfq/lib/python3.8/site-packages/pandas/core/arraylike.py:402: RuntimeWarning: divide by zero encountered in log2\n",
       "  result = getattr(ufunc, method)(*inputs, **kwargs)\n"
      ]
     },
@@ -1488,11 +1495,6 @@
      "name": "stdout",
      "output_type": "stream",
      "text": [
-      "directLFQ (fragions)\n",
-      "YEAST num:3097 median_FC:1.4 STD:0.32\n",
-      "\n",
-      "HUMAN num:8571 median_FC:-0.025 STD:0.1\n",
-      "\n",
       "directLFQ (fragions top3)\n",
       "YEAST num:3097 median_FC:1.5 STD:0.32\n",
       "\n",
@@ -1504,8 +1506,6 @@
      "name": "stderr",
      "output_type": "stream",
      "text": [
-      "/Users/constantin/opt/anaconda3/envs/directlfq/lib/python3.8/site-packages/pandas/core/arraylike.py:402: RuntimeWarning: divide by zero encountered in log2\n",
-      "  result = getattr(ufunc, method)(*inputs, **kwargs)\n",
       "/Users/constantin/opt/anaconda3/envs/directlfq/lib/python3.8/site-packages/pandas/core/arraylike.py:402: RuntimeWarning: divide by zero encountered in log2\n",
       "  result = getattr(ufunc, method)(*inputs, **kwargs)\n"
      ]
@@ -1513,10 +1513,10 @@
     {
      "data": {
       "text/plain": [
-       "<matplotlib.legend.Legend at 0x7faae53d5a30>"
+       "<matplotlib.legend.Legend at 0x7f95d93f6b20>"
       ]
      },
-     "execution_count": 13,
+     "execution_count": 12,
      "metadata": {},
      "output_type": "execute_result"
     },
@@ -1597,7 +1597,7 @@
   },
   {
    "cell_type": "code",
-   "execution_count": 14,
+   "execution_count": 13,
    "metadata": {},
    "outputs": [
     {
@@ -1607,7 +1607,7 @@
      "traceback": [
       "\u001b[0;31m---------------------------------------------------------------------------\u001b[0m",
       "\u001b[0;31mNameError\u001b[0m                                 Traceback (most recent call last)",
-      "Cell \u001b[0;32mIn[14], line 2\u001b[0m\n\u001b[1;32m      1\u001b[0m \u001b[38;5;28;01mimport\u001b[39;00m \u001b[38;5;21;01mpandas\u001b[39;00m \u001b[38;5;28;01mas\u001b[39;00m \u001b[38;5;21;01mpd\u001b[39;00m\n\u001b[0;32m----> 2\u001b[0m diann_df \u001b[38;5;241m=\u001b[39m pd\u001b[38;5;241m.\u001b[39mread_csv(\u001b[43minput_file\u001b[49m, sep\u001b[38;5;241m=\u001b[39m\u001b[38;5;124m\"\u001b[39m\u001b[38;5;130;01m\\t\u001b[39;00m\u001b[38;5;124m\"\u001b[39m)\n",
+      "Cell \u001b[0;32mIn[13], line 2\u001b[0m\n\u001b[1;32m      1\u001b[0m \u001b[38;5;28;01mimport\u001b[39;00m \u001b[38;5;21;01mpandas\u001b[39;00m \u001b[38;5;28;01mas\u001b[39;00m \u001b[38;5;21;01mpd\u001b[39;00m\n\u001b[0;32m----> 2\u001b[0m diann_df \u001b[38;5;241m=\u001b[39m pd\u001b[38;5;241m.\u001b[39mread_csv(\u001b[43minput_file\u001b[49m, sep\u001b[38;5;241m=\u001b[39m\u001b[38;5;124m\"\u001b[39m\u001b[38;5;130;01m\\t\u001b[39;00m\u001b[38;5;124m\"\u001b[39m)\n",
       "\u001b[0;31mNameError\u001b[0m: name 'input_file' is not defined"
      ]
     }