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Error loading a Synchro-PASEF method in timsControl #18

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kronigert opened this issue Jul 18, 2024 · 0 comments
Open

Error loading a Synchro-PASEF method in timsControl #18

kronigert opened this issue Jul 18, 2024 · 0 comments

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@kronigert
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A py_diAID generated Synchro-PASEF method created the following error when loaded into timsControl:

image

The cause of the error message was that the second and fourth diagonal-PASEF scan had a slightly different slope (m=1560.1282051282 instead of m=1560) compared to the first and third diagonal-PASEF scan. This cannot be handled by timsControl.

image

After adapting the end-values of the second and fourth diagonal-PASEF scan manually from 1317.2 to 1317.1 and 1379.7 to 1379.6 respectively. I was able to use the file afterwards in timsControl.

Is there maybe a rounding-error somewhere in the code that caused the slightly different slopes?

Best,
Tobias

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