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MSStatsshiny OFFLINE not able to Process DIANN input #95

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bluemooninvestor opened this issue Aug 31, 2024 · 2 comments
Open

MSStatsshiny OFFLINE not able to Process DIANN input #95

bluemooninvestor opened this issue Aug 31, 2024 · 2 comments

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@bluemooninvestor
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I am getting the following error.

Listening on http://127.0.0.1:7770
[1] "bio"
[1] "Protein"
Reached in get_data
File type is diann
[1] "C:\Users\Damin\AppData\Local\Temp\Rtmp0oRxfa/a5f9dddb71fd92168bc210a0/0.tsv"
INFO [2024-08-31 23:20:29] ** Raw data from DIANN imported successfully.
Warning: Error in [.data.table: column(s) not found: [FragmentQuantCorrected]
3: runApp
2: print.shiny.appobj
1:

I am loading the DIANN report.tsv. It does't indeed contain FragmentQuantCorrected. It contains Fragment.Quant.Raw and Fragment.Correlations. Am I missing something. I am on DIANN 1.9.

Kindly help. I am supposing MSStatsshiny does the DIANNtoMSStats convert function itself.

@bidiman
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bidiman commented Oct 15, 2024

Hi,

I had the same problem. MSStatsshiny accept only "report.tsv" from DIA-NN 1.8.1, a version that display the column Fragment.Quant.Corrected in the report.tsv file.

@bidiman
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bidiman commented Oct 17, 2024

Hi,

Finally, you can use report.tsv from DIA-NN 1.9.1 by changing then name of the column "Fragment.Quant.Raw" by "Fragment.Quant.Corrected" as suggested by V. Demichev

vdemichev/DiaNN#1088

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