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<h2>4DN consortium recommended cell lines</h2>
<ul>
<li>Tier 1: small set of cells provided to each 4DN member to be able to use and provide data for all studies.</li>
<li>Tier 2: large set of cells based on popular usage for specific projects.</li>
</ul>
By clicking on a cell line name you can access more information from the <a target="_blank" href="http://data.4dnucleome.org/">4DN Data Portal</a>, including SOPs. <br/>
<br/>
<br/>
<table class="cellLines">
<tr style="background-color:#4BAEE5">
<th width="20%">Tier 1</th>
<th width="50%">Description</th>
<th width="20%">Provider</th>
<th width="10%">Availability</th>
</tr>
<tr>
<td label="cellLineName"><a class="ex1" target="_blank" href="https://data.4dnucleome.org/biosources/4DNSRV3SKQ8M/"><b>H1-ESC (WA01)</b></a></td>
<td label="description">Human embryonic stem cells (male)</td>
<td label="provider">WiCell</td>
<td class="center" label="availability">✔</td>
</tr>
<tr>
<td label="cellLineName"><a class="ex1" target="_blank" href="https://data.4dnucleome.org/biosources/4DNSRH17RFKR/"><b>GM12878</b></a></td>
<td label="description">EBV-Immortalized B-lymphocytes (female)</td>
<td label="provider">Coriell</td>
<td class="center" label="availability">✔</td>
</tr>
<tr>
<td label="cellLineName"><a class="ex1" target="_blank" href="https://data.4dnucleome.org/biosources/4DNSRI51KRED/"><b>IMR90</b></a></td>
<td label="description">Human Fetal Lung Fibroblast</td>
<td label="provider">ATCC</td>
<td class="center" label="availability">✔</td>
</tr>
<tr>
<td label="cellLineName"><a class="ex1" target="_blank" href="https://data.4dnucleome.org/biosources/4DNSRC6ZVYVP/"><b>HFF-hTert-clone 6</b></a></td>
<td label="description">hTert-immortalized human foreskin fibroblasts (male)</td>
<td label="provider">Job Dekker Lab</td>
<td class="center" label="availability">✔</td>
</tr>
<tr>
<td label="cellLineName"><a class="ex1" target="_blank" href="https://data.4dnucleome.org/biosources/4DNSR7GFFYBK/"><b>WTC-11</b></a></td>
<td label="description">Induced pluripotent stem cells (iPSc male)</td>
<td label="provider">Bruce Conklin Lab</td>
<td class="center" label="availability">✔</td>
</tr>
</table>
<table class="cellLines">
<tr style="background-color:#4EAC5B">
<th width="20%">Tier 2</th>
<th width="50%">Description</th>
<th width="20%">Provider</th>
<th width="10%">Availability</th>
</tr>
<tr>
<td label="cellLineName"><a class="ex1" target="_blank" href="https://data.4dnucleome.org/biosources/4DNSRMG5APUM/"><b>F121-9</b></a></td>
<td label="description">Mouse Cast-129 F1 hybrid embryonic stem cells (female)</td>
<td label="provider">David Gilbert Lab</td>
<td class="center" label="availability">✔</td>
</tr>
<tr>
<td label="cellLineName"><a class="ex1" target="_blank" href="https://data.4dnucleome.org/biosources/4DNSRTNKUDSA/"><b>F123</b></a></td>
<td label="description">Mouse Cast-129 F1 hybrid embryonic stem cells (male)</td>
<td label="provider">Bing Ren Lab</td>
<td class="center" label="availability">✔</td>
</tr>
<tr>
<td label="cellLineName"><a class="ex1" target="_blank" href="https://data.4dnucleome.org/biosources/4DNSRCVNIBZG/"><b>HAP1</b></a></td>
<td label="description">Haploid derivative of chronic myelogenous leukemia</td>
<td label="provider">Bas van Steensel Lab</td>
<td class="center" label="availability">✔</td>
</tr>
<tr>
<td label="cellLineName"><a class="ex1" target="_blank" href="https://data.4dnucleome.org/biosources/4DNSR9WBB58Z/"><b>H9</b></a></td>
<td label="description">Human embryonic stem cells (female)</td>
<td label="provider">Wicell</td>
<td class="center" label="availability">✔</td>
</tr>
<tr>
<td label="cellLineName"><a class="ex1" target="_blank" href="https://data.4dnucleome.org/biosources/4DNSRHGVFSRJ/"><b>hTERT-RPE</b></a></td>
<td label="description">hTERT immortalized retina pigmented epithelium (female)</td>
<td label="provider">ATCC</td>
<td class="center" label="availability">✔</td>
</tr>
<tr>
<td label="cellLineName"><a class="ex1" target="_blank" href="https://data.4dnucleome.org/biosources/4DNSRWQFKJYO/"><b>K562</b></a></td>
<td label="description">Chronic myelogenous leukemia (female)</td>
<td label="provider">ATCC</td>
<td class="center" label="availability">✔</td>
</tr>
<tr>
<td label="cellLineName"><a class="ex1" target="_blank" href="https://data.4dnucleome.org/biosources/4DNSRVF4XB1F/"><b>HEK293</b></a></td>
<td label="description">Human embryonic kidney (female)</td>
<td label="provider">ATCC</td>
<td class="center" label="availability">✔</td>
</tr>
<tr>
<td label="cellLineName"><a class="ex1" target="_blank" href="https://data.4dnucleome.org/biosources/4DNSR3V9ST6H/"><b>U2OS</b></a></td>
<td label="description">Osteosarcoma cells (female)</td>
<td label="provider">ATCC</td>
<td class="center" label="availability">✔</td>
</tr>
<tr>
<td label="cellLineName"><a class="ex1" target="_blank" href="https://data.4dnucleome.org/biosources/4DNSRHAXQ8PG/"><b>JM8.N4</b></a></td>
<td label="description">Mouse Embryonic Stem Cells</td>
<td label="provider">Komp</td>
<td class="center" label="availability">✔</td>
</tr>
<tr>
<td label="cellLineName"><a class="ex1" target="_blank" href="https://data.4dnucleome.org/biosources/4DNSRMYUIVGD/"><b>HCT 116</b></a></td>
<td label="description">Human Colorectal carcinoma</td>
<td label="provider">ATCC</td>
<td class="center" label="availability">✔</td>
</tr>
<tr>
<td label="cellLineName"><a class="ex1" target="_blank" href="https://data.4dnucleome.org/biosources/4DNSR4B3PFLN/"><b>G4</b></a></td>
<td label="description">Mouse Embryonic Stem Cells</td>
<td label="provider">Carol Ware</td>
<td class="center" label="availability">✔</td>
</tr>
</table>
<h2>Eligibility information for PIs who are new to 4DN Cell Repository</h2>
All current Phase II PIs have been approved by the Phase II 4DN Cells WG to receive a single vial of each of our commercial repository stocked Tier1 cell lines, including: H1 (WiCell), GM12878 (Coriell), and IMR90 (ATCC). Additional vials will require separate approval from the Cells WG.<br/><br/>
<b>NOTE:</b> All PIs are reminded to stock the cells by 4DN standards and to follow the 4DN approved SOP for all cell lines. When data will be submitted to the DCIC, they will need to verify that the cells have been expanded in these conditions that are posted on the Cells WG Wiki page, and the appropriate metadata will need to be recorded at the time of cell harvest in order for datasets to receive the gold standard flag.
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