Error while pulling sv-pipeline docker image

[nitha26]

Hi,

I have 100s of CRAM files which was aligned to hg38, and interested to call structural variation. Tried to use docker since it supports hg38 version reference. I get an error while pulling sv docker image in the Centos7 machine.

#docker pull halllab/cadd-b38-v1-6 Using default tag: latest Error response from daemon: manifest for halllab/cadd-b38-v1-6:latest not found: manifest unknown: manifest unknown

Next try:

Because of this issue, tried to run to call SVs by wdl script. The Readme file instructs to use Cromwell version 25 or later (These scripts can be launched using Cromwell (version 25 or later) to execute wdl script. I think version 25 is much older because of the latest release version is 53.1. Which is the stable and newer version of Cromwell can be used to execute WDL script sv-pipeline for SV calling of CRAM files which are aligned to GRCh38?

I am new to this, could anyone help me how to call SVs form CRAM files, tried to install SVtools seprately, it's getting python2 compatible issues. Please suggest to me which type of workflow is best (wdl, docker, or SVtools) to identify the SVs.

Thanks in advance.
Nitha