diff --git a/scripts/LaunchMetrics.py b/scripts/LaunchMetrics.py index db0fd1c..0573165 100644 --- a/scripts/LaunchMetrics.py +++ b/scripts/LaunchMetrics.py @@ -138,7 +138,6 @@ def dragen_rna_alignment_and_metrics(sample, run, sample_parameters, rna_directo # get the correct path for the reference if (sample_parameters["GTAG"] == "GRCh38"): - # rna_path = "/igo/work/igo/dragen_hash_tables/hg38_alt_masked_graph_v2+cnv+graph+rna-8-1644018559" rna_path = "/igo/work/igo/dragen_hash_tables/4.2/hg38-alt_masked.cnv.graph.hla.rna-9-r3.0-1" else: rna_path = "/igo/work/igo/dragen_hash_tables/4.2/{}".format(sample_parameters["GTAG"]) @@ -147,7 +146,7 @@ def dragen_rna_alignment_and_metrics(sample, run, sample_parameters, rna_directo launch_dragen_rna = "/opt/edico/bin/dragen -f -r {} --fastq-list {} --fastq-list-sample-id {} -a {} --intermediate-results-dir /staging/temp --enable-map-align true --enable-sort true --enable-bam-indexing true --enable-map-align-output true --output-format BAM --enable-rna true --enable-duplicate-marking true --enable-rna-quantification true --output-file-prefix {} --output-directory {} ".format(rna_path, fastq_list, sample.sample_id, sample_parameters["GTF"], sample.sample_id, rna_directory) - bsub_launch_dragen_rna = "bsub -J {0}{1} -o {0}{1}.out -cwd \"{2}\" -m \"id01\" -q dragen -n 48 -M 4 {3}".format(rna_dragen_job_name_header, sample.sample_id, rna_directory, launch_dragen_rna) + bsub_launch_dragen_rna = "bsub -J {0}{1} -o {0}{1}.out -cwd \"{2}\" -m \"id01 id02 id03\" -q dragen -n 48 -M 4 {3}".format(rna_dragen_job_name_header, sample.sample_id, rna_directory, launch_dragen_rna) print(bsub_launch_dragen_rna) call(bsub_launch_dragen_rna, shell = True) @@ -176,14 +175,13 @@ def dragen(sample, run, sample_parameters, work_directory, dragen_directory, fas # get the correct path for the reference if (sample_parameters["GTAG"] == "GRCh38"): - # dragen_path = "/igo/work/igo/dragen_hash_tables/hg38_alt_masked_graph_v2+cnv+graph+rna-8-1644018559" dragen_path = "/igo/work/igo/dragen_hash_tables/4.2/hg38-alt_masked.cnv.graph.hla.rna-9-r3.0-1" else: dragen_path = "/igo/work/igo/dragen_hash_tables/4.2/{}".format(sample_parameters["GTAG"]) metric_file_prefix = "{}___P{}___{}___{}".format(run, sample.project[8:], sample.sample_id, sample_parameters["GTAG"]) launch_dragen = "/opt/edico/bin/dragen --ref-dir {} --fastq-list {} --fastq-list-sample-id {} --intermediate-results-dir /staging/temp --output-directory {} --output-file-prefix {} --enable-sort true --enable-duplicate-marking true --bin_memory 50000000000".format(dragen_path, fastq_list, sample.sample_id, dragen_directory, sample.sample_id) - bsub_launch_dragen = "bsub -J {0}{1} -o {0}{1}.out -cwd \"{2}\" -m \"id01\" -q dragen -n 48 -M 4 {3}".format(dragen_job_name_header, sample.sample_id, dragen_directory, launch_dragen) + bsub_launch_dragen = "bsub -J {0}{1} -o {0}{1}.out -cwd \"{2}\" -m \"id01 id02 id03\" -q dragen -n 48 -M 4 {3}".format(dragen_job_name_header, sample.sample_id, dragen_directory, launch_dragen) print(bsub_launch_dragen) call(bsub_launch_dragen, shell = True)