From 8e43acd459d2fe72d096e4e468341fdecc3e9f1c Mon Sep 17 00:00:00 2001 From: nf-core-bot Date: Thu, 19 Nov 2020 13:47:35 +0000 Subject: [PATCH 01/81] Template update for nf-core/tools version 1.12 --- .github/ISSUE_TEMPLATE/bug_report.md | 7 +- .github/ISSUE_TEMPLATE/config.yml | 8 + .github/ISSUE_TEMPLATE/feature_request.md | 6 + .github/workflows/awsfulltest.yml | 8 +- .github/workflows/awstest.yml | 3 +- .github/workflows/branch.yml | 2 +- .github/workflows/ci.yml | 22 +- .github/workflows/linting.yml | 14 +- .github/workflows/linting_comment.yml | 29 + .github/workflows/push_dockerhub_dev.yml | 28 + ...ckerhub.yml => push_dockerhub_release.yml} | 15 +- .gitignore | 9 +- CHANGELOG.md | 56 +- Dockerfile | 4 +- README.md | 42 +- assets/nf-core-bacass_logo.png | Bin 519 -> 12605 bytes assets/nf-core-bacass_social_preview.png | Bin 48134 -> 0 bytes assets/nf-core-bacass_social_preview.svg | 448 ----------- assets/sendmail_template.txt | 2 +- assets/test_config_dfast.py | 284 ------- bin/scrape_software_versions.py | 56 +- conf/base.config | 141 ++-- conf/test.config | 17 +- conf/test_dfast.config | 25 - conf/test_full.config | 16 +- conf/test_hybrid.config | 18 - conf/test_long.config | 22 - conf/test_long_miniasm.config | 22 - docs/README.md | 3 +- docs/containers.md | 5 - docs/images/bandage.png | Bin 281480 -> 0 bytes docs/images/fastqc.png | Bin 133836 -> 0 bytes docs/images/icarus.png | Bin 78784 -> 0 bytes docs/images/kraken2.png | Bin 51151 -> 0 bytes docs/images/nanoplot.png | Bin 845605 -> 0 bytes docs/images/nf-core-bacass_logo.png | Bin 21520 -> 428 bytes docs/images/nfcore-bacass_logo.png | Bin 15840 -> 0 bytes docs/images/nfcore-bacass_logo.svg | 205 ----- docs/images/prokka.png | Bin 106426 -> 0 bytes docs/images/quast.png | Bin 98153 -> 0 bytes docs/output.md | 163 +--- docs/usage.md | 45 +- environment.yml | 24 +- main.nf | 724 +++--------------- nextflow.config | 37 +- nextflow_schema.json | 197 ++--- 46 files changed, 477 insertions(+), 2230 deletions(-) create mode 100644 .github/ISSUE_TEMPLATE/config.yml create mode 100644 .github/workflows/linting_comment.yml create mode 100644 .github/workflows/push_dockerhub_dev.yml rename .github/workflows/{push_dockerhub.yml => push_dockerhub_release.yml} (68%) delete mode 100644 assets/nf-core-bacass_social_preview.png delete mode 100644 assets/nf-core-bacass_social_preview.svg delete mode 100644 assets/test_config_dfast.py delete mode 100644 conf/test_dfast.config delete mode 100644 conf/test_hybrid.config delete mode 100644 conf/test_long.config delete mode 100644 conf/test_long_miniasm.config delete mode 100644 docs/containers.md delete mode 100644 docs/images/bandage.png delete mode 100644 docs/images/fastqc.png delete mode 100644 docs/images/icarus.png delete mode 100644 docs/images/kraken2.png delete mode 100644 docs/images/nanoplot.png delete mode 100644 docs/images/nfcore-bacass_logo.png delete mode 100644 docs/images/nfcore-bacass_logo.svg delete mode 100644 docs/images/prokka.png delete mode 100644 docs/images/quast.png diff --git a/.github/ISSUE_TEMPLATE/bug_report.md b/.github/ISSUE_TEMPLATE/bug_report.md index 5d654575..5a3ee9d4 100644 --- a/.github/ISSUE_TEMPLATE/bug_report.md +++ b/.github/ISSUE_TEMPLATE/bug_report.md @@ -1,10 +1,15 @@ +--- +name: Bug report +about: Report something that is broken or incorrect +labels: bug +--- + diff --git a/.github/ISSUE_TEMPLATE/config.yml b/.github/ISSUE_TEMPLATE/config.yml new file mode 100644 index 00000000..eab75cde --- /dev/null +++ b/.github/ISSUE_TEMPLATE/config.yml @@ -0,0 +1,8 @@ +blank_issues_enabled: false +contact_links: + - name: Join nf-core + url: https://nf-co.re/join + about: Please join the nf-core community here + - name: "Slack #bacass channel" + url: https://nfcore.slack.com/channels/bacass + about: Discussion about the nf-core/bacass pipeline diff --git a/.github/ISSUE_TEMPLATE/feature_request.md b/.github/ISSUE_TEMPLATE/feature_request.md index 78f110cf..327248ec 100644 --- a/.github/ISSUE_TEMPLATE/feature_request.md +++ b/.github/ISSUE_TEMPLATE/feature_request.md @@ -1,3 +1,9 @@ +--- +name: Feature request +about: Suggest an idea for the nf-core website +labels: enhancement +--- + -The pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool to run tasks across multiple computing infrastructures in a portable manner. It comes with docker or singularity containers as well as conda environments, making installation trivial and results highly reproducible. + ```bash + nextflow run nf-core/bacass -profile --input '*_R{1,2}.fastq.gz' --genome GRCh37 + ``` + +See [usage docs](https://nf-co.re/bacass/usage) for all of the available options when running the pipeline. ## Documentation -The nf-core/bacass pipeline comes with documentation about the pipeline, found in the `docs/` directory: +The nf-core/bacass pipeline comes with documentation about the pipeline: [usage](https://nf-co.re/bacass/usage) and [output](https://nf-co.re/bacass/output). -The nf-core/bacass pipeline comes with documentation about the pipeline which you can read at [https://nf-core/bacass/docs](https://nf-core/bacass/docs) or find in the [`docs/` directory](docs). + ## Credits @@ -53,7 +56,8 @@ For further information or help, don't hesitate to get in touch on the [Slack `# ## Citation -If you use nf-core/bacass for your analysis, 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diff --git a/assets/test_config_dfast.py b/assets/test_config_dfast.py deleted file mode 100644 index 2e47c4e7..00000000 --- a/assets/test_config_dfast.py +++ /dev/null @@ -1,284 +0,0 @@ -#! /usr/bin/env python -# coding:utf8 -# Imported from DFAST repository to provide default config file -import multiprocessing - - -class Config: - GENOME_FASTA = "@@APP_ROOT@@/example/test.genome.fna" - WORK_DIR = "RESULT_TEST" - - CPU = 1 - FORCE_OVERWRITE = True - DEBUG = False - - GENOME_CONFIG = { - # Set 'complete' to True to annotate a complete genome. - # If 'use_original_name' is set to True, the leading non-space letters in the Fasta header line will be used as a sequence name. - # Otherwise, sequences will be renamed as 'Sequence001, Sequence002, ...'. - # If 'sort_by_length' is set to True, sequences will be sorted so that longer sequences come first. - # In a draft genome, sequences shorter than 'minimum_length' will be eliminated. - - "complete": False, - "use_original_name": False, # If set to True, the first word in the Fasta header line will be used as a sequence name. - "sort_by_length": True, - "minimum_length": 200 - } - - GENOME_SOURCE_INFORMATION = { - # These attributes are reflected in the source feature, - # and do not affect the annotation results. - - "organism": "", - "strain": "", - "seq_names": "", - "seq_types": "", - "seq_topologies": "", - "additional_modifiers": "", - } - - LOCUS_TAG_SETTINGS = { - "locus_tag_prefix": "LOCUS", - "step": 10, - "use_separate_tags": True, # If set to `True`, locus_tags are assigned separately according to feature type. - "symbols": {"CDS": "", "rRNA": "r", "tRNA": "t", "tmRNA": "tm"} - # "symbols": {"CDS": "", "rRNA": "r", "tRNA": "t", "tmRNA": "tm", "nc_rna": "nc", "misc_rna": "misc"} - } - - FEATURE_ADJUSTMENT = { - "remove_partial_features": True, # True: enabled, False: disabled - - "remove_overlapping_features": True, # True: enabled, False: disabled - "feature_type_priority": ["assembly_gap", "CRISPR", ("tmRNA", "tRNA", "rRNA"), "CDS"], - - "merge_cds": False, # True: enabled, False: disabled - "tool_type_priority": {"MGA": 0, "Prodigal": 1}, - } - - OUTPUT_RESULT = { - # output verbosity level for .gff and .gbk - "verbosity": 3 # 1: minimum, 2: standard, 3: rich - } - - DDBJ_SUBMISSION = { - "enabled": True, - "output_verbosity": 1, - "metadata_file": None - } - - GENBANK_SUBMISSION = { - "enabled": True, - "center_name": "", # Genome Center tag for GenBank submission - "output_verbosity": 1, - } - - - STRUCTURAL_ANNOTATION = [ - { - # GAP is a Gap Annotation Process that identifies gap regions (N's or n's runs) in the sequence. - "tool_name": "GAP", - "enabled": True, - "options": { - "len_cutoff": 5, # Gaps shorter than len_cutoff are ignored. - "linkage_evidence": "paired-ends", # You can change this as you like. - "gap_type": "within scaffold" # You can change this as you like. - }, - }, - { - # MetaGeneAnnotator for CDS prediction - "tool_name": "MGA", - "enabled": True, - "options": {"cmd_options": "-s"}, # -s for single species, -m for multiple species - }, - { - # Aragorn for tRNA and tmRNA prediction - "tool_name": "Aragorn", - "enabled": False, - "options": { - "gcode": "-gcbact", # -gcbact for Bacterial/Plant Chloroplast genetic code, "-gcstd" for standard genetic code. - "cmd_options": "-l", # DFAST assumes linear sequences as input. - }, - }, - { - # tRNAscan-SE for tRNA prediction. By default, this is disabled. - # Please insall tRNAscan-SE and put it in your PATH to enable this. - "tool_name": "tRNAscan", - "enabled": False, - "options": { - "model": "--bact", # --bact, --arch, --organ, --general - "cmd_options": "" - }, - }, - { - # Barrnap for rRNA prediction - "tool_name": "Barrnap", - "enabled": False, - "options": { - # Currently, Barrnap will run with default settings. - # You can set parameters such as --reject and --lencutoff to cmd_options. - # "cmd_options": "--reject 0.4 --lencutoff 0.6" - }, - }, - { - # RNAmmer for rRNA prediction. By default, this is disabled. - # Please insall RNAmmer and put it in your PATH to enable this. - "tool_name": "RNAmmer", - "enabled": False, - "options": { - "model": "bac", # arc/bac/euk - "cmd_options": "" - }, - }, - { - # CRT for CRISPR detection - # CRT runs on JavaVM. Depending on your system, it requires additional parameters to run. - # Example - # "java_options": "-Xmx256m" - "tool_name": "CRT", - "enabled": False, - "options": { - "jar_file": "@@APP_ROOT@@/bin/common/CRT1.2-CLI.jar", - "java_options": "", # options passed to java. e.g. -Xmx256m - "cmd_options": "", # cmd_options passed to CRT. - }, - }, - { - # Prodigal for CDS prediction - # By default Prodigal is disabled. To enable this, also set MGA disabled or enable merge_cds in FEATURE_ADJUSTMENT. - "tool_name": "Prodigal", - "enabled": False, - "options": { - "transl_table": 11, - "cmd_options": "", - }, - }, - ] - - FUNCTIONAL_ANNOTATION = [ - # Fucntional annotation steps will be conducted in the order specified in this list. - # You can swithch enabled/disabled, change the order, or add new steps. - - { - # OrthoSearch (All-vs-all pairwise alignment between each reference genome to assign orthologous genes) - # Normally, this should be run before other annotation steps. - # In the default workflow, it is disabled. You can enable this by using the "--references" option. - "component_name": "OrthoSearch", - "enabled": False, - "options": { - "skipAnnotatedFeatures": False, - "evalue_cutoff": 1e-6, - "qcov_cutoff": 75, - "scov_cutoff": 75, - "aligner": "ghostx", # ghostx, ghostz, or blastp - "aligner_options": {}, # Normally, leave this empty. (Current version does not use this option.) - "references": [ - ] - }, - }, - { - # By default, this is disabled. - # If you want to add your original databases to be searched in prior to default DB, - # set 'enabled' to True and specify 'database' - # The database file must be in a DFAST reference format, - # and DB indexing must be done using the 'reference_util.py' script. - "component_name": "DBsearch", - "enabled": False, - "options": { - "skipAnnotatedFeatures": True, - "evalue_cutoff": 1e-6, - "qcov_cutoff": 75, - "scov_cutoff": 75, - "aligner": "ghostx", # ghostx, ghostz or blastp - "aligner_options": {}, # Normally, leave this empty. (Current version does not use this option.) - "database": "", - }, - }, - { - # Sequence homology search against the default DB. - "component_name": "DBsearch", - "enabled": True, - "options": { - "skipAnnotatedFeatures": True, - "evalue_cutoff": 1e-6, - "qcov_cutoff": 75, - "scov_cutoff": 75, - "aligner": "ghostx", # ghostz, ghostx or blastp - "aligner_options": {}, # Normally, leave this empty. (Current version does not use this option.) - "database": "@@APP_ROOT@@/db/protein/DFAST-default.ref", - }, - }, - { - # This is for homology search against a large-sized reference database, - # such as pre-formatted Blast databases like RefSeq Protein. - "component_name": "BlastSearch", - "enabled": False, - "options": { - "skipAnnotatedFeatures": False, - "evalue_cutoff": 1e-6, - "qcov_cutoff": 75, - "scov_cutoff": 75, - "aligner": "blastp", # must be blastp - "aligner_options": {}, # Normally, leave this empty. (Current version does not use this option.) - "dbtype": "auto", # Must be either of auto/ncbi/uniprot/plain - "database": "", - }, - }, - { - # Pseudo/frame-shifted genes detection based on the upstream homology search results. - # This also detects translation exceptions to selenocystein/pyrrolysine. - "component_name": "PseudoGeneDetection", - "enabled": True, - "options": { - "skipAnnotatedFeatures": False, - "extension": 300, - "scov_cutoff": 85, - }, - }, - { - # Search against a profile-HMM database using hmmscan. - # In the standard workflow, TIGRFAM HMM library will be searched. - "component_name": "HMMscan", - "enabled": False, - "options": { - "skipAnnotatedFeatures": True, - "evalue_cutoff": 1e-6, - "database": "@@APP_ROOT@@/db/hmm/TIGRFAMs_15.0_HMM.LIB", - "db_name": "TIGR", - }, - }, - { - # You can add another HMMscan, if you want. - "component_name": "HMMscan", - "enabled": False, - "options": { - "skipAnnotatedFeatures": False, - "evalue_cutoff": 1e-6, - "db_name": "", # eg 'Pfam', - "database": "" # eg '@@APP_ROOT@@/db/hmm/Pfam-A.hmm' - }, - }, - { - # Search against Conserved Domain Database (CDD) using RPS-BLAST - # In the standard workflow, COG functional categories will be assigned by this step. - "component_name": "CDDsearch", - "enabled": False, - "options": { - "skipAnnotatedFeatures": False, - "evalue_cutoff": 1e-6, - "database": "@@APP_ROOT@@/db/cdd/Cog", - "rpsbproc_data": "@@APP_ROOT@@/bin/common/rpsbproc_data", # Do not change this. - }, - }, - { - # You can add another CDDsearch, if you want. - "component_name": "CDDsearch", - "enabled": False, - "options": { - "skipAnnotatedFeatures": False, - "evalue_cutoff": 1e-6, - "database": "", # eg @@APP_ROOT@@/db/cdd/Prk - "rpsbproc_data": "@@APP_ROOT@@/bin/common/rpsbproc_data", # Do not change this. - }, - }, - - ] diff --git a/bin/scrape_software_versions.py b/bin/scrape_software_versions.py index adf3cbca..1ea01f65 100755 --- a/bin/scrape_software_versions.py +++ b/bin/scrape_software_versions.py @@ -1,21 +1,40 @@ #!/usr/bin/env python from __future__ import print_function -import os +from collections import OrderedDict +import re -results = {} -version_files = [x for x in os.listdir('.') if x.endswith('.version.txt')] -for version_file in version_files: +# TODO nf-core: Add additional regexes for new tools in process get_software_versions +regexes = { + "nf-core/bacass": ["v_pipeline.txt", r"(\S+)"], + "Nextflow": ["v_nextflow.txt", r"(\S+)"], + "FastQC": ["v_fastqc.txt", r"FastQC v(\S+)"], + "MultiQC": ["v_multiqc.txt", r"multiqc, version (\S+)"], +} +results = OrderedDict() +results["nf-core/bacass"] = 'N/A' +results["Nextflow"] = 'N/A' +results["FastQC"] = 'N/A' +results["MultiQC"] = 'N/A' - software = version_file.replace('.version.txt','') - if software == 'pipeline': - software = 'nf-core/bacass' +# Search each file using its regex +for k, v in regexes.items(): + try: + with open(v[0]) as x: + versions = x.read() + match = re.search(v[1], versions) + if match: + results[k] = "v{}".format(match.group(1)) + except IOError: + results[k] = False - with open(version_file) as fin: - version = fin.read().strip() - results[software] = version +# Remove software set to false in results +for k in list(results): + if not results[k]: + del results[k] # Dump to YAML -print (''' +print( + """ id: 'software_versions' section_name: 'nf-core/bacass Software Versions' section_href: 'https://github.com/nf-core/bacass' @@ -23,12 +42,13 @@ description: 'are collected at run time from the software output.' data: |

-''') -for k,v in sorted(results.items()): - print("
{}
{}
".format(k,v)) -print ("
") +""" +) +for k, v in results.items(): + print("
{}
{}
".format(k, v)) +print(" ") # Write out regexes as csv file: -with open('software_versions.csv', 'w') as f: - for k,v in sorted(results.items()): - f.write("{}\t{}\n".format(k,v)) +with open("software_versions.csv", "w") as f: + for k, v in results.items(): + f.write("{}\t{}\n".format(k, v)) diff --git a/conf/base.config b/conf/base.config index dfcfda10..5257108d 100644 --- a/conf/base.config +++ b/conf/base.config @@ -1,106 +1,51 @@ +/* + * ------------------------------------------------- + * nf-core/bacass Nextflow base config file + * ------------------------------------------------- + * A 'blank slate' config file, appropriate for general + * use on most high performace compute environments. + * Assumes that all software is installed and available + * on the PATH. Runs in `local` mode - all jobs will be + * run on the logged in environment. + */ + process { - cpus = { check_max( 2, 'cpus' ) } - memory = { check_max( 8.GB * task.attempt, 'memory' ) } - time = { check_max( 2.h * task.attempt, 'time' ) } + // TODO nf-core: Check the defaults for all processes + cpus = { check_max( 1 * task.attempt, 'cpus' ) } + memory = { check_max( 7.GB * task.attempt, 'memory' ) } + time = { check_max( 4.h * task.attempt, 'time' ) } - errorStrategy = { task.exitStatus in [1,143,137,104,134,139] ? 'retry' : 'finish' } - maxRetries = 3 + errorStrategy = { task.exitStatus in [143,137,104,134,139] ? 'retry' : 'finish' } + maxRetries = 1 maxErrors = '-1' - withLabel:'small'{ - cpus = { check_max( 2, 'cpus' ) } - memory = { check_max( 1.GB * task.attempt, 'memory' ) } - time = { check_max( 1.h * task.attempt, 'time' ) } - } - - withLabel:'medium' { - cpus = { check_max( 8, 'cpus' ) } - memory = { check_max( 8.GB * task.attempt, 'memory' ) } - time = { check_max( 8.h * task.attempt, 'time' ) } - } - - withLabel:'large'{ - cpus = { check_max( 32, 'cpus' ) } - memory = { check_max( 350.GB * task.attempt, 'memory' ) } - time = { check_max( 160.h * task.attempt, 'time' ) } - } - - withName:'quast'{ - cpus = { check_max( 2, 'cpus' ) } - memory = { check_max( 1.GB * task.attempt, 'memory' ) } - time = { check_max( 1.h * task.attempt, 'time' ) } - container = 'quay.io/biocontainers/quast:5.0.2--py37pl526hb5aa323_2' - } - withName:'adapter_trimming'{ - cpus = { check_max( 12, 'cpus' ) } - memory = { check_max( 32.GB * task.attempt, 'memory' ) } - time = { check_max( 1.h * task.attempt, 'time' ) } - container = 'quay.io/biocontainers/porechop:0.2.4--py38hed8969a_1' + // Process-specific resource requirements + // NOTE - Only one of the labels below are used in the fastqc process in the main script. + // If possible, it would be nice to keep the same label naming convention when + // adding in your processes. + // TODO nf-core: Customise requirements for specific processes. + // See https://www.nextflow.io/docs/latest/config.html#config-process-selectors + withLabel:process_low { + cpus = { check_max( 2 * task.attempt, 'cpus' ) } + memory = { check_max( 14.GB * task.attempt, 'memory' ) } + time = { check_max( 6.h * task.attempt, 'time' ) } + } + withLabel:process_medium { + cpus = { check_max( 6 * task.attempt, 'cpus' ) } + memory = { check_max( 42.GB * task.attempt, 'memory' ) } + time = { check_max( 8.h * task.attempt, 'time' ) } + } + withLabel:process_high { + cpus = { check_max( 12 * task.attempt, 'cpus' ) } + memory = { check_max( 84.GB * task.attempt, 'memory' ) } + time = { check_max( 10.h * task.attempt, 'time' ) } + } + withLabel:process_long { + time = { check_max( 20.h * task.attempt, 'time' ) } + } + withName:get_software_versions { + cache = false } - withName:'unicycler'{ - cpus = { check_max( 28, 'cpus' ) } - memory = { check_max( 450.GB * task.attempt, 'memory' ) } - time = { check_max( 72.h * task.attempt, 'time' ) } - container = 'quay.io/biocontainers/unicycler:0.4.4--py38h8162308_3' - } - - withName:'dfast'{ - cpus = { check_max( 8, 'cpus' ) } - memory = { check_max( 16.GB * task.attempt, 'memory' ) } - time = { check_max( 8.h * task.attempt, 'time' ) } - container = 'quay.io/biocontainers/dfast:1.2.10--h8b12597_0' - } - - withName:'medaka'{ - cpus = { check_max( 8, 'cpus' ) } - memory = { check_max( 250.GB * task.attempt, 'memory' ) } - time = { check_max( 8.h * task.attempt, 'time' ) } - container = 'quay.io/biocontainers/medaka:1.1.2--py38hfcf0ad1_0' - } - - withName:'canu_assembly'{ - cpus = { check_max( 32, 'cpus' ) } - memory = { check_max( 350.GB * task.attempt, 'memory' ) } - time = { check_max( 160.h * task.attempt, 'time' ) } - } - - withName:'pycoqc' { - cpus = { check_max( 8, 'cpus' ) } - memory = { check_max( 8.GB * task.attempt, 'memory' ) } - time = { check_max( 8.h * task.attempt, 'time' ) } - container = 'quay.io/biocontainers/pycoqc:2.5.0.23--py_0' - } - - withName:'prokka'{ - cpus = { check_max( 32, 'cpus' ) } - memory = { check_max( 350.GB * task.attempt, 'memory' ) } - time = { check_max( 160.h * task.attempt, 'time' ) } - container = 'quay.io/biocontainers/prokka:1.14.6--pl526_0' - } - - withName:'nanopolish'{ - cpus = { check_max( 32, 'cpus' ) } - memory = { check_max( 350.GB * task.attempt, 'memory' ) } - time = { check_max( 160.h * task.attempt, 'time' ) } - container = 'quay.io/biocontainers/mulled-v2-47609127678014b991aafdfe3c9141852f17fee7:11970b07a8fd8f9441c3235a979d527c7df52d34-0' - } - - withName:'nanoplot' { - cpus = { check_max( 8, 'cpus' ) } - memory = { check_max( 8.GB * task.attempt, 'memory' ) } - time = { check_max( 8.h * task.attempt, 'time' ) } - container = 'quay.io/biocontainers/nanoplot:1.32.1--py_0' - } - - - - params { - // Defaults only, expecting to be overwritten - max_memory = 64.GB - max_cpus = 32 - max_time = 24.h - igenomes_base = 's3://ngi-igenomes/igenomes/' - } } diff --git a/conf/test.config b/conf/test.config index 6a25a110..89bd6fba 100644 --- a/conf/test.config +++ b/conf/test.config @@ -14,12 +14,13 @@ params { max_cpus = 2 max_memory = 6.GB max_time = 48.h - skip_kraken2 = true - // some extra args to speed tests up - unicycler_args="--no_correct --no_pilon" - prokka_args=" --fast" - assembly_type = 'short' - skip_pycoqc = true - // Input dataset - input = 'https://raw.githubusercontent.com/nf-core/test-datasets/bacass/bacass_short.csv' + + // Input data + // TODO nf-core: Specify the paths to your test data on nf-core/test-datasets + // TODO nf-core: Give any required params for the test so that command line flags are not needed + single_end = false + input_paths = [ + ['Testdata', ['https://github.com/nf-core/test-datasets/raw/exoseq/testdata/Testdata_R1.tiny.fastq.gz', 'https://github.com/nf-core/test-datasets/raw/exoseq/testdata/Testdata_R2.tiny.fastq.gz']], + ['SRR389222', ['https://github.com/nf-core/test-datasets/raw/methylseq/testdata/SRR389222_sub1.fastq.gz', 'https://github.com/nf-core/test-datasets/raw/methylseq/testdata/SRR389222_sub2.fastq.gz']] + ] } diff --git a/conf/test_dfast.config b/conf/test_dfast.config deleted file mode 100644 index 9138ed47..00000000 --- a/conf/test_dfast.config +++ /dev/null @@ -1,25 +0,0 @@ -/* - * ------------------------------------------------- - * Nextflow config file for running tests - * ------------------------------------------------- - * Defines bundled input files and everything required - * to run a fast and simple test. Use as follows: - * nextflow run nf-core/bacass -profile test_dfast - */ - -params { - config_profile_name = 'Test profile with dfast as annotation tool' - config_profile_description = 'Minimal test dataset to check pipeline function, using dfast instead of prokka for annotation' - // Limit resources so that this can run on Travis - max_cpus = 2 - max_memory = 6.GB - max_time = 48.h - skip_kraken2 = true - // some extra args to speed tests up - unicycler_args="--no_correct --no_pilon" - annotation_tool = 'dfast' - assembly_type = 'short' - skip_pycoqc = true - // Input dataset - input = 'https://raw.githubusercontent.com/nf-core/test-datasets/bacass/bacass_short.csv' -} \ No newline at end of file diff --git a/conf/test_full.config b/conf/test_full.config index d5d28082..24b436f0 100644 --- a/conf/test_full.config +++ b/conf/test_full.config @@ -6,17 +6,17 @@ * to run a full size pipeline test. Use as follows: * nextflow run nf-core/bacass -profile test_full, */ -includeConfig 'test.config' params { config_profile_name = 'Full test profile' config_profile_description = 'Full test dataset to check pipeline function' - // some extra args to speed tests up - prokka_args=" --fast" - canu_args='stopOnLowCoverage=0 minInputCoverage=0' - assembly_type='long' - skip_polish = true - // Input data - input = 'https://raw.githubusercontent.com/nf-core/test-datasets/bacass/bacass_long.csv' + // Input data for full size test + // TODO nf-core: Specify the paths to your full test data ( on nf-core/test-datasets or directly in repositories, e.g. SRA) + // TODO nf-core: Give any required params for the test so that command line flags are not needed + single_end = false + input_paths = [ + ['Testdata', ['https://github.com/nf-core/test-datasets/raw/exoseq/testdata/Testdata_R1.tiny.fastq.gz', 'https://github.com/nf-core/test-datasets/raw/exoseq/testdata/Testdata_R2.tiny.fastq.gz']], + ['SRR389222', ['https://github.com/nf-core/test-datasets/raw/methylseq/testdata/SRR389222_sub1.fastq.gz', 'https://github.com/nf-core/test-datasets/raw/methylseq/testdata/SRR389222_sub2.fastq.gz']] + ] } diff --git a/conf/test_hybrid.config b/conf/test_hybrid.config deleted file mode 100644 index 40bd1abb..00000000 --- a/conf/test_hybrid.config +++ /dev/null @@ -1,18 +0,0 @@ -/* - * ------------------------------------------------- - * Nextflow config file for running tests - * ------------------------------------------------- - * Defines bundled input files and everything required - * to run a fast and simple test. Use as follows: - * nextflow run nf-core/bacass -profile test_long - */ -includeConfig 'test.config' -params { - config_profile_name = 'Test profile for long and short read data' - config_profile_description = 'Minimal test dataset to check pipeline function' - // some extra args to speed tests up - assembly_type='hybrid' - prokka_args=" --fast" - // Input data - input = 'https://raw.githubusercontent.com/nf-core/test-datasets/bacass/bacass_hybrid.csv' -} \ No newline at end of file diff --git a/conf/test_long.config b/conf/test_long.config deleted file mode 100644 index 7cbc76f9..00000000 --- a/conf/test_long.config +++ /dev/null @@ -1,22 +0,0 @@ -/* - * ------------------------------------------------- - * Nextflow config file for running tests - * ------------------------------------------------- - * Defines bundled input files and everything required - * to run a fast and simple test. Use as follows: - * nextflow run nf-core/bacass -profile test_long - */ -includeConfig 'test.config' - -params { - config_profile_name = 'Test profile for long-read data' - config_profile_description = 'Minimal test dataset to check pipeline function' - - // some extra args to speed tests up - prokka_args=" --fast" - canu_args='stopOnLowCoverage=0 minInputCoverage=0' - assembly_type='long' - skip_polish = true - // Input data - input = 'https://raw.githubusercontent.com/nf-core/test-datasets/bacass/bacass_long.csv' -} \ No newline at end of file diff --git a/conf/test_long_miniasm.config b/conf/test_long_miniasm.config deleted file mode 100644 index 93dee34f..00000000 --- a/conf/test_long_miniasm.config +++ /dev/null @@ -1,22 +0,0 @@ -/* - * ------------------------------------------------- - * Nextflow config file for running tests - * ------------------------------------------------- - * Defines bundled input files and everything required - * to run a fast and simple test. Use as follows: - * nextflow run nf-core/bacass -profile test_long - */ -includeConfig 'test.config' - -params { - config_profile_name = 'Test profile for long-read data' - config_profile_description = 'Minimal test dataset to check pipeline function' - - // some extra args to speed tests up - prokka_args=" --fast" - assembly_type='long' - assembler = 'miniasm' - skip_polish = true - // Input data - input = 'https://raw.githubusercontent.com/nf-core/test-datasets/bacass/bacass_long_miniasm.csv' -} \ No newline at end of file diff --git a/docs/README.md b/docs/README.md index 88f11b6d..fc6b898b 100644 --- a/docs/README.md +++ b/docs/README.md @@ -1,11 +1,10 @@ # nf-core/bacass: Documentation -The nf-core/bacass documentation is split into the following files: +The nf-core/bacass documentation is split into the following pages: * [Usage](usage.md) * An overview of how the pipeline works, how to run it and a description of all of the different command-line flags. * [Output](output.md) * An overview of the different results produced by the pipeline and how to interpret them. -* [Containers](containers.md) You can find a lot more documentation about installing, configuring and running nf-core pipelines on the website: [https://nf-co.re](https://nf-co.re) diff --git a/docs/containers.md b/docs/containers.md deleted file mode 100644 index df229e06..00000000 --- a/docs/containers.md +++ /dev/null @@ -1,5 +0,0 @@ -# Containers - -## `nfcore/bacass` container - -Some of the tools we use in bacass are inherently relying on software dependencies that are not installable in the same environment due to dependency conflicts. In these cases we utilize biocontainers. With a potential switch to Nextflow DSLv2 and therefore nextflow modules, we intend to have separated containers for all processes. 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zEqkV*u0>S7r8%@INlRsRw0^dQ)>DpE)q;sDGjbBqv4mIy+T2-|RI$VcU*}cLFR_uiysuJ(HB~{gtDG&5J}_>{tWxxg%*JJll_cA zcNOHXUiP&fu7(Q3A}WYk%Gtd><9%I8!@$)dq407Sd#Uttd~~Rq2lLvC7^Uyu$an3p z0^_$U&uk?sekL#TEKUqhok4jFS3caUT<|?S{1ym7E8l*~oo8nX-Sm_xjdSV) zy7P5?W&{f8Sbd9XW+lwRV>RH89WNiP?RBh+!0xE=W`m-dV>-A%y{e;kY>0Z7Sj6uy zok9&I($HC-DW(-+(t%QEFa`@XtUUTg2COY|VJ1)|S4;E0iqFV07uq;*t$&O#dZQdb{@?Md1=3VmakPEq>AEZs(Wz!;YgdMlbP(xD*_4YAY%e?n?eIkcM zU#l_tKO6rX;Be8^5FW#u_CG9X5z5GtzR0(ypgl`|e)I3cImmgw5KO*sC7aV^?c;DY zZ-I71=3K5TvrEDD_agT<`LQ+&#Tl#pm3YjZrq0;fd&6O5frm-oYfN&MPFGMS*K0M6&U+xKWAc4juxdQT=1Goicl-U`u@ zWhl3!t6&Z(+^$Yyerg0KdDHl`yZB1wwqIzJ{ga_qp9KNP7lFS#og?$CRd;ntx4J8M z%H^T3qVGx5h*&0^21_$JuQN(Ht$z?Z%4yeU8*q1|GPWK9e+teVE+}gTZHp@y( z`Ob+Z@2stRw3J-*8aAX8v3_EP-u*gxZ^0*0eX#QgqjBKdt*KK$2&v!xa1Z9Wyqug& zuysI*ex5d`D!N4XF|sbj6hwWY3P~lx?|(A+5?-lBY}q(`U)9AZYGuM@VW5|#MzfM{ zFdMqlcxG(A`bg%KqB;@T!OtK=R9x=MQ3|V`ZJDYZ78}z(FT?8p|sY~nad<*a6 zkBN$z0oO2dMh+`JdY|yxX%UWHM`74zq8{VJA>${eQ9%r>UL^@g1I55%i@JTg=R3ZQ z1~om;&Z#jiX|_h~N`z_h|GU8XU9%iDzU|3OIdOgKyYNaDC~Rx{Z1k4-?#^au{Vshw zb5m6GPBoQEU7M>)O6MF#)8R0S@XM2M&s!(?Ec@(_ZKw zSh;bHED7v?F2u#p!3-F9Q5A0l*sEH9j}T00?fX?g;Pu$KMiRcbxpE&6$5It}m?4V) zRAE{Orm*ueqDcqDiQp$M?=2?&8Od=2SgJ(t3cO<3?+hkp8PtBAn2;~HMDW5-TE3k> zAdWp?{pbN(;~y|FQ}i5eh|@PiXVg~%(c-bMVC2%ceV&Cq;O3Zu0tW-)(Ip^v52+Y5 VnQKbCKf^@-= + ## Pipeline overview The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes data using the following steps: -* [nf-core/bacass: Output](#nf-corebacass-output) - * [Pipeline overview](#pipeline-overview) - * [Quality trimming and QC](#quality-trimming-and-qc) - * [Short Read Trimming](#short-read-trimming) - * [Short Read RAW QC](#short-read-raw-qc) - * [Long Read Trimming](#long-read-trimming) - * [Long Read RAW QC](#long-read-raw-qc) - * [Taxonomic classification](#taxonomic-classification) - * [Kraken2 report screenshot](#kraken2-report-screenshot) - * [Assembly Output](#assembly-output) - * [Assembly Visualization with Bandage](#assembly-visualization-with-bandage) - * [Assembly QC with QUAST](#assembly-qc-with-quast) - * [Annotation with Prokka](#annotation-with-prokka) - * [Report](#report) - * [Pipeline information](#pipeline-information) - -## Quality trimming and QC - -### Short Read Trimming - -This step quality trims the end of reads, removes degenerate or too short reads and if needed, -combines reads coming from multiple sequencing runs. - -**Output directory: `{sample_id}/trimming/shortreads/`** - -* `*.fastq.gz` - * trimmed (and combined reads) - -### Short Read RAW QC - -This step runs FastQC which produces -general quality metrics on your (trimmed) samples and plots them. - -**Output directory: `{sample_id}/trimming/shortreads/`** - -* `*_fastqc.html` - * FastQC report, containing quality metrics for your trimmed reads -* `*_fastqc.zip` - * zip file containing the FastQC report, tab-delimited data file and plot images - -For further reading and documentation see the [FastQC help](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/Help/). - -![FastQC report](images/fastqc.png) - -### Long Read Trimming - -This step performs long read trimming on Nanopore input (if provided). - -**Output directory: `{sample_id}/trimming/longreads/`** - -* `trimmed.fastq` - * The trimmed FASTQ file - -### Long Read RAW QC - -These steps perform long read QC for input data (if provided). - -**Output directory: `{sample_id}/QC_Longreads/`** - -* `NanoPlot` -* `PycoQC` - -Please refer to the documentation of [NanoPlot](https://github.com/wdecoster/NanoPlot) and [PycoQC](https://a-slide.github.io/pycoQC/) if you want to know more about the plots created by these tools. - -Example plot from Nanoplot: - -![Nanoplot](images/nanoplot.png) - -## Taxonomic classification - -This QC step classifies your reads using [Kraken2](https://ccb.jhu.edu/software/kraken2/) a k-mer based approach. This helps to identify samples that have purity -issues. Ideally you will not want to assemble reads from samples that are contaminated or contain -multiple species. If you like to visualize the report, try -[Pavian](https://github.com/fbreitwieser/pavian) or [Krakey](http://krakey.info/). +* [FastQC](#fastqc) - Read quality control +* [MultiQC](#multiqc) - Aggregate report describing results from the whole pipeline +* [Pipeline information](#pipeline-information) - Report metrics generated during the workflow execution -**Output directory: `{sample}/`** +## FastQC -* `*_kraken2.report` - * Classification in the Kraken(1) report format. See - [webpage](http://ccb.jhu.edu/software/kraken/MANUAL.html#sample-reports) for more details +[FastQC](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/) gives general quality metrics about your sequenced reads. It provides information about the quality score distribution across your reads, per base sequence content (%A/T/G/C), adapter contamination and overrepresented sequences. -### Kraken2 report screenshot +For further reading and documentation see the [FastQC help pages](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/Help/). -![Kraken2 report](images/kraken2.png) - -## Assembly Output - -Trimmed reads are assembled with [Unicycler](https://github.com/rrwick/Unicycler) in `short` or `hybrid` assembly modes. For long-read assembly, there are also `canu` and `miniasm` available. -Unicycler is a pipeline on its own, which at least for Illumina reads mainly acts as a frontend to Spades with added polishing steps. - -**Output directory: `{sample_id}/unicycler`** - -* `{sample}_assembly.fasta` - * Final assembly -* `{sample}_assembly.gfa` - * Final assembly in Graphical Fragment Assembly (GFA) format -* `{sample}_unicycler.log` - * Log file summarizing steps and intermediate results on the Unicycler execution - -Check out the [Unicycler documentation](https://github.com/rrwick/Unicycler) for more information on Unicycler output. - -**Output directory: `{sample_id}/canu`** - -Check out the [Canu documentation](https://canu.readthedocs.io/en/latest/index.html) for more information on Canu output. - -**Output directory: `{sample_id}/miniasm`** - -* `consensus` - * The consensus sequence created by `miniasm` - -Check out the [Miniasm documentation](https://github.com/lh3/miniasm) for more information on Miniasm output. - -## Assembly Visualization with Bandage - -The GFA file produced in the assembly step with Unicycler can be used to visualise the assembly graph, which is -done here with [Bandage](https://rrwick.github.io/Bandage/). We highly recommend to run the Bandage GUI for more versatile visualisation options (annotations etc). - -**Output directory: `{sample_id}/unicycler`** - -* `{sample}_assembly.png` - * Bandage visualization of assembly - -![Assembly visualization](images/bandage.png) - -## Assembly QC with QUAST - -The assembly QC is performed with [QUAST](http://quast.sourceforge.net/quast). -It reports multiple metrics including number of contigs, N50, lengths etc in form of an html report. -It further creates an HTML file with integrated contig viewer (Icarus). - -**Output directory: `{sample_id}/QUAST`** - -* `icarus.html` - * QUAST's contig browser as HTML -* `report.html` - * QUAST assembly QC as HTML report - -![QUAST QC](images/quast.png) - -![Icarus](images/icarus.png) - -## Annotation with Prokka - -The assembly is annotated with [Prokka](https://github.com/tseemann/prokka) which acts as frontend -for several annotation tools and includes rRNA and ORF predictions. See [its documentation](https://github.com/tseemann/prokka#output-files) for a full description of all output files. - -**Output directory: `{sample_id}/{sample_id}_annotation`** +**Output files:** -![Prokka annotation](images/prokka.png) +* `fastqc/` + * `*_fastqc.html`: FastQC report containing quality metrics for your untrimmed raw fastq files. +* `fastqc/zips/` + * `*_fastqc.zip`: Zip archive containing the FastQC report, tab-delimited data file and plot images. -## Report +> **NB:** The FastQC plots displayed in the MultiQC report shows _untrimmed_ reads. They may contain adapter sequence and potentially regions with low quality. -Some pipeline results are visualised by [MultiQC](http://multiqc.info), which is a visualisation tool that generates a single HTML report summarising all samples in your project. Further statistics are available in within the report data directory. +## MultiQC [MultiQC](http://multiqc.info) is a visualization tool that generates a single HTML report summarizing all samples in your project. Most of the pipeline QC results are visualised in the report and further statistics are available in the report data directory. diff --git a/docs/usage.md b/docs/usage.md index 73bf960f..9cfcb7d2 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -1,4 +1,4 @@ -# nf-core/bacass: Usage +# nf-core/bacass: Usage ## :warning: Please read this documentation on the nf-core website: [https://nf-co.re/bacass/usage](https://nf-co.re/bacass/usage) @@ -6,29 +6,14 @@ ## Introduction -* [General Nextflow info](#general-nextflow-info) -* [Running the pipeline](#running-the-pipeline) - * [Updating the pipeline](#updating-the-pipeline) - * [Reproducibility](#reproducibility) -* [Main Nextflow arguments](#main-nextflow-arguments) - * [`-profile`](#-profile) - -## General Nextflow info - -Nextflow handles job submissions on SLURM or other environments, and supervises running the jobs. Thus the Nextflow process must run until the pipeline is finished. We recommend that you put the process running in the background through `screen` / `tmux` or similar tool. Alternatively you can run nextflow within a cluster job submitted your job scheduler. - -It is recommended to limit the Nextflow Java virtual machines memory. We recommend adding the following line to your environment (typically in `~/.bashrc` or `~./bash_profile`): - -```bash -NXF_OPTS='-Xms1g -Xmx4g' -``` + ## Running the pipeline The typical command for running the pipeline is as follows: ```bash -nextflow run nf-core/bacass --input design.tsv -profile docker +nextflow run nf-core/bacass --input '*_R{1,2}.fastq.gz' -profile docker ``` This will launch the pipeline with the `docker` configuration profile. See below for more information about profiles. @@ -58,26 +43,38 @@ First, go to the [nf-core/bacass releases page](https://github.com/nf-core/bacas This version number will be logged in reports when you run the pipeline, so that you'll know what you used when you look back in the future. -## Main Nextflow arguments +## Core Nextflow arguments + +> **NB:** These options are part of Nextflow and use a _single_ hyphen (pipeline parameters use a double-hyphen). ### `-profile` Use this parameter to choose a configuration profile. Profiles can give configuration presets for different compute environments. Several generic profiles are bundled with the pipeline which instruct the pipeline to use software packaged using different methods (Docker, Singularity, Podman, Conda) - see below. -If `-profile` is not specified at all the pipeline will be run locally and expects all software to be installed and available on the `PATH`. -* `awsbatch` - * A generic configuration profile to be used with AWS Batch. +> We highly recommend the use of Docker or Singularity containers for full pipeline reproducibility, however when this is not possible, Conda is also supported. + +The pipeline also dynamically loads configurations from [https://github.com/nf-core/configs](https://github.com/nf-core/configs) when it runs, making multiple config profiles for various institutional clusters available at run time. For more information and to see if your system is available in these configs please see the [nf-core/configs documentation](https://github.com/nf-core/configs#documentation). + +Note that multiple profiles can be loaded, for example: `-profile test,docker` - the order of arguments is important! +They are loaded in sequence, so later profiles can overwrite earlier profiles. + +If `-profile` is not specified, the pipeline will run locally and expect all software to be installed and available on the `PATH`. This is _not_ recommended. + * `docker` - * A generic configuration profile to be used with [Docker](http://docker.com/) - * Pulls software from DockerHub: [`nfcore/bacass`](http://hub.docker.com/r/nfcore/bacass/) + * A generic configuration profile to be used with [Docker](https://docker.com/) + * Pulls software from Docker Hub: [`nfcore/bacass`](https://hub.docker.com/r/nfcore/bacass/) * `singularity` * A generic configuration profile to be used with [Singularity](https://sylabs.io/docs/) * Pulls software from Docker Hub: [`nfcore/bacass`](https://hub.docker.com/r/nfcore/bacass/) * `podman` * A generic configuration profile to be used with [Podman](https://podman.io/) * Pulls software from Docker Hub: [`nfcore/bacass`](https://hub.docker.com/r/nfcore/bacass/) +* `conda` + * Please only use Conda as a last resort i.e. when it's not possible to run the pipeline with Docker, Singularity or Podman. + * A generic configuration profile to be used with [Conda](https://conda.io/docs/) + * Pulls most software from [Bioconda](https://bioconda.github.io/) * `test` * A profile with a complete configuration for automated testing * Includes links to test data so needs no other parameters diff --git a/environment.yml b/environment.yml index 2fd763e3..0e316597 100644 --- a/environment.yml +++ b/environment.yml @@ -6,20 +6,10 @@ channels: - bioconda - defaults dependencies: - - python=3.7.3 - #Stuff for the documentation output - - conda-forge::markdown=3.3 - - conda-forge::pymdown-extensions=8.0.1 - - conda-forge::pygments=2.7.1 - #Other dependencies - - fastqc=0.11.9 - - multiqc=1.9 - - skewer=0.2.2 - - kraken2=2.0.9beta - # Nanopore analysis stuff - - conda-forge::parallel=20200922 - - miniasm=0.3_r179 - - racon=1.4.13 - - minimap2=2.17 - - canu=2.0 - - h5py=2.10.0 #until the pycoqc recipe has been updated + - conda-forge::python=3.7.3 + - conda-forge::markdown=3.1.1 + - conda-forge::pymdown-extensions=6.0 + - conda-forge::pygments=2.5.2 + # TODO nf-core: Add required software dependencies here + - bioconda::fastqc=0.11.8 + - bioconda::multiqc=1.7 diff --git a/main.nf b/main.nf index 46b079f8..10359131 100644 --- a/main.nf +++ b/main.nf @@ -10,37 +10,35 @@ */ def helpMessage() { + // TODO nf-core: Add to this help message with new command line parameters log.info nfcoreHeader() log.info""" + Usage: The typical command for running the pipeline is as follows: - nextflow run nf-core/bacass --input input.csv --kraken2db 'path-to-kraken2db' -profile docker + nextflow run nf-core/bacass --input '*_R{1,2}.fastq.gz' -profile docker Mandatory arguments: - -profile Configuration profile to use. Can use multiple (comma separated) - Available: conda, docker, singularity, awsbatch, test and more. - --input The design file used for running the pipeline in TSV format. - - Pipeline arguments: - --assembler Default: "Unicycler", Available: "Canu", "Miniasm", "Unicycler". Short & Hybrid assembly always runs "Unicycler". - --assembly_type Default: "Short", Available: "Short", "Long", "Hybrid". - --kraken2db Path to Kraken2 Database directory - --prokka_args Advanced: Extra arguments to Prokka (quote and add leading space) - --unicycler_args Advanced: Extra arguments to Unicycler (quote and add leading space) - --canu_args Advanced: Extra arguments for Canu assembly (quote and add leading space) + --input [file] Path to input data (must be surrounded with quotes) + -profile [str] Configuration profile to use. Can use multiple (comma separated) + Available: conda, docker, singularity, test, awsbatch, and more + + Options: + --genome [str] Name of iGenomes reference + --single_end [bool] Specifies that the input is single-end reads + + References If not specified in the configuration file or you wish to overwrite any of the references + --fasta [file] Path to fasta reference Other options: - --outdir The output directory where the results will be saved - --email Set this parameter to your e-mail address to get a summary e-mail with details of the run sent to you when the workflow exits - -name Name for the pipeline run. If not specified, Nextflow will automatically generate a random mnemonic. - - Skipping options: - --skip_annotation Skips the annotation with Prokka - --skip_kraken2 Skips the read classification with Kraken2 - --skip_polish Skips polishing long-reads with Nanopolish or Medaka - --skip_pycoqc Skips long-read raw signal QC + --outdir [file] The output directory where the results will be saved + --publish_dir_mode [str] Mode for publishing results in the output directory. Available: symlink, rellink, link, copy, copyNoFollow, move (Default: copy) + --email [email] Set this parameter to your e-mail address to get a summary e-mail with details of the run sent to you when the workflow exits + --email_on_fail [email] Same as --email, except only send mail if the workflow is not successful + --max_multiqc_email_size [str] Threshold size for MultiQC report to be attached in notification email. If file generated by pipeline exceeds the threshold, it will not be attached (Default: 25MB) + -name [str] Name for the pipeline run. If not specified, Nextflow will automatically generate a random mnemonic AWSBatch options: --awsqueue [str] The AWSBatch JobQueue that needs to be set when running on AWSBatch @@ -55,14 +53,26 @@ if (params.help) { exit 0 } -if(! params.skip_kraken2){ - if(params.kraken2db){ - kraken2db = file(params.kraken2db) - } else { - exit 1, "Missing Kraken2 DB arg" - } +/* + * SET UP CONFIGURATION VARIABLES + */ + +// Check if genome exists in the config file +if (params.genomes && params.genome && !params.genomes.containsKey(params.genome)) { + exit 1, "The provided genome '${params.genome}' is not available in the iGenomes file. Currently the available genomes are ${params.genomes.keySet().join(", ")}" } +// TODO nf-core: Add any reference files that are needed +// Configurable reference genomes +// +// NOTE - THIS IS NOT USED IN THIS PIPELINE, EXAMPLE ONLY +// If you want to use the channel below in a process, define the following: +// input: +// file fasta from ch_fasta +// +params.fasta = params.genome ? params.genomes[ params.genome ].fasta ?: false : false +if (params.fasta) { ch_fasta = file(params.fasta, checkIfExists: true) } + // Has the run name been specified by the user? // this has the bonus effect of catching both -name and --name custom_runName = params.name @@ -82,109 +92,68 @@ if (workflow.profile.contains('awsbatch')) { } // Stage config files -ch_multiqc_config = file("$baseDir/assets/multiqc_config.yaml", checkIfExists: true) +ch_multiqc_config = file("$projectDir/assets/multiqc_config.yaml", checkIfExists: true) ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath(params.multiqc_config, checkIfExists: true) : Channel.empty() -ch_output_docs = file("$baseDir/docs/output.md", checkIfExists: true) -ch_output_docs_images = file("$baseDir/docs/images/", checkIfExists: true) +ch_output_docs = file("$projectDir/docs/output.md", checkIfExists: true) +ch_output_docs_images = file("$projectDir/docs/images/", checkIfExists: true) -//Check whether we have a design file as input set -if(!params.input){ - exit 1, "Missing Design File - please see documentation how to create one." +/* + * Create a channel for input read files + */ +if (params.input_paths) { + if (params.single_end) { + Channel + .from(params.input_paths) + .map { row -> [ row[0], [ file(row[1][0], checkIfExists: true) ] ] } + .ifEmpty { exit 1, "params.input_paths was empty - no input files supplied" } + .into { ch_read_files_fastqc; ch_read_files_trimming } + } else { + Channel + .from(params.input_paths) + .map { row -> [ row[0], [ file(row[1][0], checkIfExists: true), file(row[1][1], checkIfExists: true) ] ] } + .ifEmpty { exit 1, "params.input_paths was empty - no input files supplied" } + .into { ch_read_files_fastqc; ch_read_files_trimming } + } } else { - //Design file looks like this - // ID R1 R2 Long-ReadFastQ Fast5Path GenomeSize - // ID is required, everything else (can!) be optional and causes some pipeline components to turn off! - // Tapping the parsed input design to multiple channels to get some data to specific downstream processes that don't need full information! Channel - .fromPath(params.input) - .splitCsv(header: true, sep:'\t') - .map { col -> - def id = "${col.ID}" - def r1 = returnFile("${col.R1}") - def r2 = returnFile("${col.R2}") - def lr = returnFile("${col.LongFastQ}") - def f5 = returnFile("${col.Fast5}") - def genome_size = "${col.GenomeSize}" - tuple(id,r1,r2,lr,f5,genome_size) - } - .dump(tag: "input") - .tap {ch_all_data; ch_all_data_for_fast5; ch_all_data_for_genomesize} - .map { id,r1,r2,lr,f5,gs -> - tuple(id,r1,r2) - } - .filter{ id,r1,r2 -> - r1 != 'NA' && r2 != 'NA'} - //Filter to get rid of R1/R2 that are NA - .into {ch_for_short_trim; ch_for_fastqc} - //Dump long read info to different channel! - ch_all_data - .map { id, r1, r2, lr, f5, genomeSize -> - tuple(id, file(lr)) - } - .dump(tag: 'longinput') - .into {ch_for_long_trim; ch_for_nanoplot; ch_for_pycoqc; ch_for_nanopolish; ch_for_long_fastq} - - //Dump fast5 to separate channel - ch_all_data_for_fast5 - .map { id, r1, r2, lr, f5, genomeSize -> - tuple(id, f5) - } - .filter {id, fast5 -> - fast5 != 'NA' - } - .into {ch_fast5_for_pycoqc; ch_fast5_for_nanopolish} - - //Dump genomeSize to separate channel, too - ch_all_data_for_genomesize - .map { id, r1, r2, lr, f5, genomeSize -> - tuple(id,genomeSize) - } - .filter{id, genomeSize -> - genomeSize != 'NA' - } - .set {ch_genomeSize_forCanu} + .fromFilePairs(params.input, size: params.single_end ? 1 : 2) + .ifEmpty { exit 1, "Cannot find any reads matching: ${params.input}\nNB: Path needs to be enclosed in quotes!\nIf this is single-end data, please specify --single_end on the command line." } + .into { ch_read_files_fastqc; ch_read_files_trimming } } // Header log info log.info nfcoreHeader() def summary = [:] -if(workflow.revision) summary['Pipeline Release'] = workflow.revision -summary['Pipeline Name'] = 'nf-core/bacass' -summary['Run Name'] = custom_runName ?: workflow.runName -summary['Assembler Method'] = params.assembler -summary['Assembly Type'] = params.assembly_type -if (params.kraken2db) summary['Kraken2 DB'] = params.kraken2db -summary['Extra Prokka arguments'] = params.prokka_args -summary['Extra Unicycler arguments'] = params.unicycler_args -summary['Extra Canu arguments'] = params.canu_args -if (params.skip_annotation) summary['Skip Annotation'] = params.skip_annotation -if (params.skip_kraken2) summary['Skip Kraken2'] = params.skip_kraken2 -if (params.skip_polish) summary['Skip Polish'] = params.skip_polish -if (!params.skip_polish) summary['Polish Method'] = params.polish_method -if (params.skip_pycoqc) summary['Skip PycoQC'] = params.skip_pycoqc +if (workflow.revision) summary['Pipeline Release'] = workflow.revision +summary['Run Name'] = custom_runName ?: workflow.runName +// TODO nf-core: Report custom parameters here +summary['Input'] = params.input +summary['Fasta Ref'] = params.fasta +summary['Data Type'] = params.single_end ? 'Single-End' : 'Paired-End' summary['Max Resources'] = "$params.max_memory memory, $params.max_cpus cpus, $params.max_time time per job" -if(workflow.containerEngine) summary['Container'] = "$workflow.containerEngine - $workflow.container" +if (workflow.containerEngine) summary['Container'] = "$workflow.containerEngine - $workflow.container" +summary['Output dir'] = params.outdir summary['Launch dir'] = workflow.launchDir -summary['Output dir'] = params.outdir -summary['Working dir'] = workflow.workDir -summary['Script dir'] = workflow.projectDir -summary['User'] = workflow.userName -if(workflow.profile == 'awsbatch'){ - summary['AWS Region'] = params.awsregion - summary['AWS Queue'] = params.awsqueue +summary['Working dir'] = workflow.workDir +summary['Script dir'] = workflow.projectDir +summary['User'] = workflow.userName +if (workflow.profile.contains('awsbatch')) { + summary['AWS Region'] = params.awsregion + summary['AWS Queue'] = params.awsqueue + summary['AWS CLI'] = params.awscli } summary['Config Profile'] = workflow.profile - -if(params.config_profile_description) summary['Config Description'] = params.config_profile_description -if(params.config_profile_contact) summary['Config Contact'] = params.config_profile_contact -if(params.config_profile_url) summary['Config URL'] = params.config_profile_url -if(params.email) { - summary['E-mail Address'] = params.email - summary['MultiQC maxsize'] = params.max_multiqc_email_size +if (params.config_profile_description) summary['Config Profile Description'] = params.config_profile_description +if (params.config_profile_contact) summary['Config Profile Contact'] = params.config_profile_contact +if (params.config_profile_url) summary['Config Profile URL'] = params.config_profile_url +summary['Config Files'] = workflow.configFiles.join(', ') +if (params.email || params.email_on_fail) { + summary['E-mail Address'] = params.email + summary['E-mail on failure'] = params.email_on_fail + summary['MultiQC maxsize'] = params.max_multiqc_email_size } log.info summary.collect { k,v -> "${k.padRight(18)}: $v" }.join("\n") -log.info "----------------------------------------------------" - +log.info "-\033[2m--------------------------------------------------\033[0m-" // Check the hostnames against configured profiles checkHostname() @@ -205,513 +174,66 @@ Channel.from(summary.collect{ [it.key, it.value] }) """.stripIndent() } .set { ch_workflow_summary } - -//Check compatible parameters -if(("${params.assembler}" == 'canu' || "${params.assembler}" == 'miniasm') && ("${params.assembly_type}" == 'short' || "${params.assembly_type}" == 'hybrid')){ - exit 1, "Canu and Miniasm can only be used for long read assembly and neither for Hybrid nor Shortread assembly!" -} - - -/* Trim and combine short read read-pairs per sample. Similar to nf-core vipr - */ -process trim_and_combine { - label 'medium' - - tag "$sample_id" - publishDir "${params.outdir}/${sample_id}/trimming/shortreads/", mode: params.publish_dir_mode - - input: - set sample_id, file(r1), file(r2) from ch_for_short_trim - - output: - set sample_id, file("${sample_id}_trm-cmb.R1.fastq.gz"), file("${sample_id}_trm-cmb.R2.fastq.gz") into (ch_short_for_kraken2, ch_short_for_unicycler, ch_short_for_fastqc) - // not keeping logs for multiqc input. for that to be useful we would need to concat first and then run skewer - - script: - """ - # loop over readunits in pairs per sample - pairno=0 - echo "${r1} ${r2}" | xargs -n2 | while read fq1 fq2; do - skewer --quiet -t ${task.cpus} -m pe -q 3 -n -z \$fq1 \$fq2; - done - cat \$(ls *trimmed-pair1.fastq.gz | sort) >> ${sample_id}_trm-cmb.R1.fastq.gz - cat \$(ls *trimmed-pair2.fastq.gz | sort) >> ${sample_id}_trm-cmb.R2.fastq.gz - """ -} - - -//AdapterTrimming for ONT reads -process adapter_trimming { - - publishDir "${params.outdir}/${sample_id}/trimming/longreads/", mode: params.publish_dir_mode - - when: params.assembly_type == 'hybrid' || params.assembly_type == 'long' - - input: - set sample_id, file(lr) from ch_for_long_trim - - output: - set sample_id, file('trimmed.fastq') into (ch_long_trimmed_unicycler, ch_long_trimmed_canu, ch_long_trimmed_miniasm, ch_long_trimmed_consensus, ch_long_trimmed_nanopolish, ch_long_trimmed_kraken, ch_long_trimmed_medaka) - file ("porechop.version.txt") into ch_porechop_version - - when: !('short' in params.assembly_type) - - script: - """ - porechop -i "${lr}" -t "${task.cpus}" -o trimmed.fastq - porechop --version > porechop.version.txt - """ -} - -/* - * STEP 1 - FastQC FOR SHORT READS -*/ -process fastqc { - label 'small' - tag "$sample_id" - publishDir "${params.outdir}/${sample_id}/FastQC", mode: params.publish_dir_mode - - input: - set sample_id, file(fq1), file(fq2) from ch_short_for_fastqc - - output: - file "*_fastqc.{zip,html}" into ch_fastqc_results - - script: - """ - fastqc -t ${task.cpus} -q ${fq1} ${fq2} - """ -} - -/* - * Quality check for nanopore reads and Quality/Length Plots - */ -process nanoplot { - tag "$sample_id" - publishDir "${params.outdir}/${sample_id}/QC_longreads/NanoPlot", mode: params.publish_dir_mode - - when: (params.assembly_type != 'short') - - input: - set sample_id, file(lr) from ch_for_nanoplot - - output: - file '*.png' - file '*.html' - file '*.txt' - file 'nanoplot.version.txt' into ch_nanoplot_version - - script: - """ - NanoPlot -t "${task.cpus}" --title "${sample_id}" -c darkblue --fastq ${lr} - NanoPlot --version | sed -e "s/NanoPlot //g" > nanoplot.version.txt - """ -} - - -/** Quality check for nanopore Fast5 files -*/ - -process pycoqc{ - label 'medium' - - tag "$sample_id" - publishDir "${params.outdir}/${sample_id}/QC_longreads/PycoQC", mode: params.publish_dir_mode - - when: (params.assembly_type == 'hybrid' || params.assembly_type == 'long') && !params.skip_pycoqc && fast5 - - input: - set sample_id, file(lr), file(fast5) from ch_for_pycoqc.join(ch_fast5_for_pycoqc) - - output: - set sample_id, file('sequencing_summary.txt') into ch_summary_index_for_nanopolish - file("pycoQC_${sample_id}*") - file("pycoQC.version.txt") into ch_pycoqc_version - - script: - //Find out whether the sequencing_summary already exists - if(file("${fast5}/sequencing_summary.txt").exists()){ - run_summary = '' - prefix = "${fast5}/" - } else { - run_summary = "Fast5_to_seq_summary -f $fast5 -t ${task.cpus} -s './sequencing_summary.txt' --verbose_level 2" - prefix = '' - } - //Barcodes available? - barcode_me = file("${fast5}/barcoding_sequencing.txt").exists() ? "-b ${fast5}/barcoding_sequencing.txt" : '' - """ - $run_summary - pycoQC -f "${prefix}sequencing_summary.txt" $barcode_me -o pycoQC_${sample_id}.html -j pycoQC_${sample_id}.json - pycoQC --version | sed -e "s/pycoQC v//g" > pycoQC.version.txt - """ -} - -/* Join channels for unicycler, as trimming the files happens in two separate processes for paralellization of individual steps. As samples have the same sampleID, we can simply use join() to merge the channels based on this. If we only have one of the channels we insert 'NAs' which are not used in the unicycler process then subsequently, in case of short or long read only assembly. -*/ -if(params.assembly_type == 'hybrid'){ - ch_short_for_unicycler - .join(ch_long_trimmed_unicycler) - .dump(tag: 'unicycler') - .set {ch_short_long_joint_unicycler} -} else if(params.assembly_type == 'short'){ - ch_short_for_unicycler - .map{id,R1,R2 -> - tuple(id,R1,R2,'NA')} - .dump(tag: 'unicycler') - .set {ch_short_long_joint_unicycler} -} else if(params.assembly_type == 'long'){ - ch_long_trimmed_unicycler - .map{id,lr -> - tuple(id,'NA','NA',lr)} - .dump(tag: 'unicycler') - .set {ch_short_long_joint_unicycler} -} - -/* unicycler (short, long or hybrid mode!) - */ -process unicycler { - tag "$sample_id" - publishDir "${params.outdir}/${sample_id}/unicycler", mode: params.publish_dir_mode - - when: params.assembler == 'unicycler' - - input: - set sample_id, file(fq1), file(fq2), file(lrfastq) from ch_short_long_joint_unicycler - - output: - set sample_id, file("${sample_id}_assembly.fasta") into (quast_ch, prokka_ch, dfast_ch) - file("${sample_id}_assembly.fasta") into (ch_assembly_nanopolish_unicycler,ch_assembly_medaka_unicycler) - file("${sample_id}_assembly.gfa") - file("${sample_id}_assembly.png") - file("${sample_id}_unicycler.log") - file("unicycler.version.txt") into ch_unicycler_version - - script: - if(params.assembly_type == 'long'){ - data_param = "-l $lrfastq" - } else if (params.assembly_type == 'short'){ - data_param = "-1 $fq1 -2 $fq2" - } else if (params.assembly_type == 'hybrid'){ - data_param = "-1 $fq1 -2 $fq2 -l $lrfastq" - } - - """ - unicycler $data_param --threads ${task.cpus} ${params.unicycler_args} --keep 0 -o . - mv unicycler.log ${sample_id}_unicycler.log - # rename so that quast can use the name - mv assembly.gfa ${sample_id}_assembly.gfa - mv assembly.fasta ${sample_id}_assembly.fasta - unicycler --version | sed -e "s/Unicycler v//g" > unicycler.version.txt - """ -} - -process miniasm_assembly { - label 'large' - - tag "$sample_id" - publishDir "${params.outdir}/${sample_id}/miniasm", mode: params.publish_dir_mode, pattern: 'assembly.fasta' - - input: - set sample_id, file(lrfastq) from ch_long_trimmed_miniasm - - output: - file 'assembly.fasta' into ch_assembly_from_miniasm - - when: params.assembler == 'miniasm' - - script: - """ - minimap2 -x ava-ont -t "${task.cpus}" "${lrfastq}" "${lrfastq}" > "${lrfastq}.paf" - miniasm -f "${lrfastq}" "${lrfastq}.paf" > "${lrfastq}.gfa" - awk '/^S/{print ">"\$2"\\n"\$3}' "${lrfastq}.gfa" | fold > assembly.fasta - """ -} - -//Run consensus for miniasm, the others don't need it. -process consensus { - label 'large' - - tag "$sample_id" - publishDir "${params.outdir}/${sample_id}/miniasm/consensus", mode: params.publish_dir_mode, pattern: 'assembly_consensus.fasta' - - input: - set sample_id, file(lrfastq) from ch_long_trimmed_consensus - file(assembly) from ch_assembly_from_miniasm - - output: - file 'assembly_consensus.fasta' into (ch_assembly_consensus_for_nanopolish, ch_assembly_consensus_for_medaka) - - script: - """ - minimap2 -x map-ont -t "${task.cpus}" "${assembly}" "${lrfastq}" > assembly.paf - racon -t "${task.cpus}" "${lrfastq}" assembly.paf "${assembly}" > assembly_consensus.fasta - """ -} - -process canu_assembly { - label 'large' - - tag "$sample_id" - publishDir "${params.outdir}/${sample_id}/canu", mode: params.publish_dir_mode, pattern: 'assembly.fasta' - - input: - set sample_id, file(lrfastq), val(genomeSize) from ch_long_trimmed_canu.join(ch_genomeSize_forCanu) - - output: - file 'assembly.fasta' into (assembly_from_canu_for_nanopolish, assembly_from_canu_for_medaka) - file 'canu.version.txt' into ch_canu_version - - when: params.assembler == 'canu' - - script: - """ - canu -p assembly -d canu_out \ - genomeSize="${genomeSize}" -nanopore "${lrfastq}" \ - maxThreads="${task.cpus}" merylMemory="${task.memory.toGiga()}G" \ - merylThreads="${task.cpus}" hapThreads="${task.cpus}" batMemory="${task.memory.toGiga()}G" \ - redMemory="${task.memory.toGiga()}G" redThreads="${task.cpus}" \ - oeaMemory="${task.memory.toGiga()}G" oeaThreads="${task.cpus}" \ - corMemory="${task.memory.toGiga()}G" corThreads="${task.cpus}" ${params.canu_args} - mv canu_out/assembly.contigs.fasta assembly.fasta - canu --version | sed -e "s/Canu //g" > canu.version.txt - """ -} - -/* kraken classification: QC for sample purity, only short end reads for now - */ -process kraken2 { - label 'large' - tag "$sample_id" - publishDir "${params.outdir}/${sample_id}/kraken", mode: params.publish_dir_mode - - input: - set sample_id, file(fq1), file(fq2) from ch_short_for_kraken2 - - output: - file("${sample_id}_kraken2.report") - - when: !params.skip_kraken2 - - script: - """ - # stdout reports per read which is not needed. kraken.report can be used with pavian - # braken would be nice but requires readlength and correspondingly build db - kraken2 --threads ${task.cpus} --paired --db ${kraken2db} \ - --report ${sample_id}_kraken2.report ${fq1} ${fq2} | gzip > kraken2.out.gz - """ -} - -/* kraken classification: QC for sample purity, only short end reads for now - */ -process kraken2_long { - label 'large' - tag "$sample_id" - publishDir "${params.outdir}/${sample_id}/kraken_long", mode: params.publish_dir_mode - - input: - set sample_id, file(lr) from ch_long_trimmed_kraken - - output: - file("${sample_id}_kraken2.report") - - when: !params.skip_kraken2 - - script: - """ - # stdout reports per read which is not needed. kraken.report can be used with pavian - # braken would be nice but requires readlength and correspondingly build db - kraken2 --threads ${task.cpus} --db ${kraken2db} \ - --report ${sample_id}_kraken2.report ${lr} | gzip > kraken2.out.gz - """ -} - -/* assembly qc with quast - */ -process quast { - tag {"$sample_id"} - publishDir "${params.outdir}/${sample_id}/QUAST", mode: params.publish_dir_mode - - input: - set sample_id, file(fasta) from quast_ch - - output: - // multiqc only detects a file called report.tsv. to avoid - // name clash with other samples we need a directory named by sample - file("${sample_id}_assembly_QC/") - file("${sample_id}_assembly_QC/report.tsv") into quast_logs_ch - file("quast.version.txt") into ch_quast_version - - script: - """ - quast -t ${task.cpus} -o ${sample_id}_assembly_QC ${fasta} - quast --version | sed -e "s/QUAST v//g" > quast.version.txt - """ -} - /* - * Annotation with prokka + * Parse software version numbers */ -process prokka { - - tag "$sample_id" - publishDir "${params.outdir}/${sample_id}/", mode: params.publish_dir_mode - - input: - set sample_id, file(fasta) from prokka_ch - - output: - file("${sample_id}_annotation/") - file("prokka.version.txt") into ch_prokka_version - - when: !params.skip_annotation && params.annotation_tool == 'prokka' - - script: - """ - prokka --cpus ${task.cpus} --prefix "${sample_id}" --outdir ${sample_id}_annotation ${params.prokka_args} ${fasta} - prokka --version | sed -e "s/prokka //g" > prokka.version.txt - """ -} - -process dfast { - tag "$sample_id" - publishDir "${params.outdir}/${sample_id}/", mode: params.publish_dir_mode - - input: - set sample_id, file(fasta) from dfast_ch - file (config) from Channel.value(params.dfast_config ? file(params.dfast_config) : "") - - output: - file("RESULT*") - file("dfast.version.txt") into ch_dfast_version - - when: !params.skip_annotation && params.annotation_tool == 'dfast' - - script: - """ - dfast --genome ${fasta} --config $config - dfast --version | sed -e "s/DFAST ver. //g" > dfast.version.txt - """ -} - - -//Polishes assembly using FAST5 files -process nanopolish { - tag "$assembly" - label 'large' - - publishDir "${params.outdir}/${sample_id}/nanopolish/", mode: params.publish_dir_mode, pattern: 'polished_genome.fa' - - input: - file(assembly) from ch_assembly_consensus_for_nanopolish.mix(ch_assembly_nanopolish_unicycler,assembly_from_canu_for_nanopolish) //Should take either miniasm, canu, or unicycler consensus sequence (!) - set sample_id, file(lrfastq), file(fast5) from ch_long_trimmed_nanopolish.join(ch_fast5_for_nanopolish) - - output: - file 'polished_genome.fa' - file 'nanopolish.version.txt' into ch_nanopolish_version - file 'samtools.version.txt' into ch_samtools_version - - when: !params.skip_polish && params.assembly_type == 'long' && params.polish_method != 'medaka' - - script: - """ - nanopolish index -d "${fast5}" "${lrfastq}" - minimap2 -ax map-ont -t ${task.cpus} "${assembly}" "${lrfastq}"| \ - samtools sort -o reads.sorted.bam -T reads.tmp - - samtools index reads.sorted.bam - nanopolish_makerange.py "${assembly}" | parallel --results nanopolish.results -P "${task.cpus}" nanopolish variants --consensus -o polished.{1}.vcf -w {1} -r "${lrfastq}" -b reads.sorted.bam -g "${assembly}" -t "${task.cpus}" --min-candidate-frequency 0.1 - nanopolish vcf2fasta -g "${assembly}" polished.*.vcf > polished_genome.fa - - #Versions - nanopolish --version | sed -e "s/nanopolish version //g" | head -n 1 > nanopolish.version.txt - samtools --version | sed -e "s/samtools //g" | head -n 1 > samtools.version.txt - """ -} - -//Polishes assembly -process medaka { - tag "$assembly" - label 'large' - - publishDir "${params.outdir}/${sample_id}/medaka/", mode: params.publish_dir_mode, pattern: 'polished_genome.fa' - - input: - file(assembly) from ch_assembly_consensus_for_medaka.mix(ch_assembly_medaka_unicycler,assembly_from_canu_for_medaka) //Should take either miniasm, canu, or unicycler consensus sequence (!) - set sample_id, file(lrfastq) from ch_long_trimmed_medaka +process get_software_versions { + publishDir "${params.outdir}/pipeline_info", mode: params.publish_dir_mode, + saveAs: { filename -> + if (filename.indexOf(".csv") > 0) filename + else null + } output: - file 'polished_genome.fa' - file 'medaka.version.txt' into ch_medaka_version - - when: !params.skip_polish && params.assembly_type == 'long' && params.polish_method == 'medaka' + file 'software_versions_mqc.yaml' into ch_software_versions_yaml + file "software_versions.csv" script: + // TODO nf-core: Get all tools to print their version number here """ - medaka_consensus -i ${lrfastq} -d ${assembly} -o "polished_genome.fa" -t ${task.cpus} - medaka --version | sed -e "s/medaka //g" > medaka.version.txt + echo $workflow.manifest.version > v_pipeline.txt + echo $workflow.nextflow.version > v_nextflow.txt + fastqc --version > v_fastqc.txt + multiqc --version > v_multiqc.txt + scrape_software_versions.py &> software_versions_mqc.yaml """ } /* - * Parse software version numbers + * STEP 1 - FastQC */ -process get_software_versions { - publishDir "${params.outdir}/pipeline_info", mode: params.publish_dir_mode, - saveAs: {filename -> - if (filename.indexOf(".csv") > 0) filename - else null - } +process fastqc { + tag "$name" + label 'process_medium' + publishDir "${params.outdir}/fastqc", mode: params.publish_dir_mode, + saveAs: { filename -> + filename.indexOf(".zip") > 0 ? "zips/$filename" : "$filename" + } input: - path quast_version from ch_quast_version.first().ifEmpty([]) - path porechop_version from ch_porechop_version.first().ifEmpty([]) - path pycoqc_version from ch_pycoqc_version.first().ifEmpty([]) - path unicycler_version from ch_unicycler_version.first().ifEmpty([]) - path canu_version from ch_canu_version.first().ifEmpty([]) - path prokka_version from ch_prokka_version.first().ifEmpty([]) - path dfast_version from ch_dfast_version.first().ifEmpty([]) - path nanopolish_version from ch_nanopolish_version.first().ifEmpty([]) - path samtools_version from ch_samtools_version.first().ifEmpty([]) - path nanoplot_version from ch_nanoplot_version.first().ifEmpty([]) - path medaka_version from ch_medaka_version.first().ifEmpty([]) + set val(name), file(reads) from ch_read_files_fastqc output: - file 'software_versions_mqc.yaml' into software_versions_yaml - file "software_versions.csv" + file "*_fastqc.{zip,html}" into ch_fastqc_results script: """ - #All in main container - echo $workflow.manifest.version > pipeline.version.txt - echo $workflow.nextflow.version > nextflow.version.txt - fastqc --version | sed -e "s/FastQC v//g" > fastqc.version.txt - - #Inside main container - miniasm -V > miniasm.version.txt - minimap2 --version &> minimap2.version.txt - racon --version | sed -e "s/v//g" > racon.version.txt - skewer --version | sed -e "s/skewer version://g" | sed -e 's/\\s//g' | head -n 1 > skewer.version.txt - kraken2 --version | sed -e "s/Kraken version //g" | head -n 1 > kraken2.version.txt - multiqc --version | sed -e "s/multiqc, version//g" > multiqc.version.txt - scrape_software_versions.py > software_versions_mqc.yaml + fastqc --quiet --threads $task.cpus $reads """ } /* - * STEP - MultiQC + * STEP 2 - MultiQC */ - process multiqc { - label 'small' publishDir "${params.outdir}/MultiQC", mode: params.publish_dir_mode input: file (multiqc_config) from ch_multiqc_config file (mqc_custom_config) from ch_multiqc_custom_config.collect().ifEmpty([]) - //file prokka_logs from prokka_logs_ch.collect().ifEmpty([]) - file ('quast_logs/*') from quast_logs_ch.collect().ifEmpty([]) - // NOTE unicycler and kraken not supported + // TODO nf-core: Add in log files from your new processes for MultiQC to find! file ('fastqc/*') from ch_fastqc_results.collect().ifEmpty([]) - file ('software_versions/*') from software_versions_yaml.collect() + file ('software_versions/*') from ch_software_versions_yaml.collect() file workflow_summary from ch_workflow_summary.collectFile(name: "workflow_summary_mqc.yaml") output: @@ -723,6 +245,7 @@ process multiqc { rtitle = custom_runName ? "--title \"$custom_runName\"" : '' rfilename = custom_runName ? "--filename " + custom_runName.replaceAll('\\W','_').replaceAll('_+','_') + "_multiqc_report" : '' custom_config_file = params.multiqc_config ? "--config $mqc_custom_config" : '' + // TODO nf-core: Specify which MultiQC modules to use with -m for a faster run time """ multiqc -f $rtitle $rfilename $custom_config_file . """ @@ -780,6 +303,7 @@ workflow.onComplete { email_fields['summary']['Nextflow Build'] = workflow.nextflow.build email_fields['summary']['Nextflow Compile Timestamp'] = workflow.nextflow.timestamp + // TODO nf-core: If not using MultiQC, strip out this code (including params.max_multiqc_email_size) // On success try attach the multiqc report def mqc_report = null try { @@ -802,18 +326,18 @@ workflow.onComplete { // Render the TXT template def engine = new groovy.text.GStringTemplateEngine() - def tf = new File("$baseDir/assets/email_template.txt") + def tf = new File("$projectDir/assets/email_template.txt") def txt_template = engine.createTemplate(tf).make(email_fields) def email_txt = txt_template.toString() // Render the HTML template - def hf = new File("$baseDir/assets/email_template.html") + def hf = new File("$projectDir/assets/email_template.html") def html_template = engine.createTemplate(hf).make(email_fields) def email_html = html_template.toString() // Render the sendmail template - def smail_fields = [ email: params.email, subject: subject, email_txt: email_txt, email_html: email_html, baseDir: "$baseDir", mqcFile: mqc_report, mqcMaxSize: params.max_multiqc_email_size.toBytes() ] - def sf = new File("$baseDir/assets/sendmail_template.txt") + def smail_fields = [ email: email_address, subject: subject, email_txt: email_txt, email_html: email_html, baseDir: "$projectDir", mqcFile: mqc_report, mqcMaxSize: params.max_multiqc_email_size.toBytes() ] + def sf = new File("$projectDir/assets/sendmail_template.txt") def sendmail_template = engine.createTemplate(sf).make(smail_fields) def sendmail_html = sendmail_template.toString() @@ -909,13 +433,3 @@ def checkHostname() { } } } - -// Return file if it exists, if NA is found this gets treated as a String information -static def returnFile(it) { - if(it == 'NA') { - return 'NA' - } else { - if (!file(it).exists()) exit 1, "Warning: Missing file in CSV file: ${it}, see --help for more information" - return file(it) - } -} diff --git a/nextflow.config b/nextflow.config index 765a1aa6..0a0a6c48 100644 --- a/nextflow.config +++ b/nextflow.config @@ -7,37 +7,27 @@ // Global default params, used in configs params { - input = '' + // Workflow flags + // TODO nf-core: Specify your pipeline's command line flags + genome = false + input = "data/*{1,2}.fastq.gz" + single_end = false outdir = './results' - skip_kraken2 = false - kraken2db = "" - unicycler_args = "" - prokka_args = "" - assembler = 'unicycler' //allowed are unicycler, canu, miniasm - //Short, Long or Hybrid assembly? - assembly_type = 'short' //allowed are short, long, hybrid (hybrid works only with Unicycler) - annotation_tool = 'prokka' //Default - canu_args = '' //Default no extra options, can be adjusted by the user - dfast_config = "$baseDir/assets/test_config_dfast.py" - polish_method = 'medaka' - //Skipping parts - skip_pycoqc = false - skip_annotation = false - skip_polish = false + publish_dir_mode = 'copy' // Boilerplate options name = false multiqc_config = false email = false + email_on_fail = false max_multiqc_email_size = 25.MB plaintext_email = false monochrome_logs = false help = false - publish_dir_mode = 'copy' igenomes_base = 's3://ngi-igenomes/igenomes/' - igenomes_ignore = true tracedir = "${params.outdir}/pipeline_info" + igenomes_ignore = false custom_config_version = 'master' custom_config_base = "https://raw.githubusercontent.com/nf-core/configs/${params.custom_config_version}" hostnames = false @@ -54,7 +44,7 @@ params { // Container slug. Stable releases should specify release tag! // Developmental code should specify :dev -process.container = 'nfcore/bacass:1.1.1' +process.container = 'nfcore/bacass:dev' // Load base.config by default for all pipelines includeConfig 'conf/base.config' @@ -67,7 +57,7 @@ try { } profiles { - conda { process.conda = "$baseDir/environment.yml" } + conda { process.conda = "$projectDir/environment.yml" } debug { process.beforeScript = 'echo $HOSTNAME' } docker { docker.enabled = true @@ -85,10 +75,7 @@ profiles { podman.enabled = true } test { includeConfig 'conf/test.config' } - test_dfast {includeConfig 'conf/test_dfast.config'} - test_long { includeConfig 'conf/test_long.config' } - test_long_miniasm { includeConfig 'conf/test_long_miniasm.config' } - test_hybrid { includeConfig 'conf/test_hybrid.config' } + test_full { includeConfig 'conf/test_full.config' } } // Load igenomes.config if required @@ -129,7 +116,7 @@ manifest { homePage = 'https://github.com/nf-core/bacass' description = 'Simple bacterial assembly and annotation pipeline.' mainScript = 'main.nf' - nextflowVersion = '>=19.10.0' + nextflowVersion = '>=20.04.0' version = '1.1.1' } diff --git a/nextflow_schema.json b/nextflow_schema.json index e1029c3e..b1191d1f 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -17,8 +17,14 @@ "input": { "type": "string", "fa_icon": "fas fa-dna", - "description": "The input design file for the pipeline.", - "help_text": "\nUse this to specify the location of your input design file. For example:\n\n```\n--input 'design_hybrid.tsv'\n```\n\nAn example of properly formatted input files can be found at the [nf-core/testData](https://github.com/nf-core/test-datasets/tree/bacass). Exemplarily, this is the input used for a hybrid assembly in testing:\n\n```\nID R1 R2 LongFastQ Fast5 GenomeSize\nERR044595 https://github.com/nf-core/test-datasets/raw/bacass/ERR044595_1M_1.fastq.gz https://github.com/nf-core/test-datasets/raw/bacass/ERR044595_1M_2.fastq.gz https://github.com/nf-core/test-datasets/raw/bacass/nanopore/subset15000.fq.gz NA 2.8m\n```\n\n* `ID` The identifier to use for handling the dataset e.g. sample name\n* `R1` The forward reads in case of available short-read data\n* `R2` The reverse reads in case of available short-read data\n* `LongFastQ` The long*read FastQ file with reads in FASTQ format\n* `Fast5` The folder containing the basecalled FAST5 files\n* `GenomeSize` The expected genome size of the assembly. Only used by the canu assembler.\n\nMissing values (e.g. FAST5 folder in case of short reads) can be omitted by using a `NA` in the TSV file. The pipeline will handle such cases appropriately then." + "description": "Input FastQ files.", + "help_text": "Use this to specify the location of your input FastQ files. For example:\n\n```bash\n--input 'path/to/data/sample_*_{1,2}.fastq'\n```\n\nPlease note the following requirements:\n\n1. The path must be enclosed in quotes\n2. The path must have at least one `*` wildcard character\n3. When using the pipeline with paired end data, the path must use `{1,2}` notation to specify read pairs.\n\nIf left unspecified, a default pattern is used: `data/*{1,2}.fastq.gz`" + }, + "single_end": { + "type": "boolean", + "description": "Specifies that the input is single-end reads.", + "fa_icon": "fas fa-align-center", + "help_text": "By default, the pipeline expects paired-end data. If you have single-end data, you need to specify `--single_end` on the command line when you launch the pipeline. A normal glob pattern, enclosed in quotation marks, can then be used for `--input`. For example:\n\n```bash\n--single_end --input '*.fastq'\n```\n\nIt is not possible to run a mixture of single-end and paired-end files in one run." }, "outdir": { "type": "string", @@ -35,6 +41,40 @@ } } }, + "reference_genome_options": { + "title": "Reference genome options", + "type": "object", + "fa_icon": "fas fa-dna", + "description": "Options for the reference genome indices used to align reads.", + "properties": { + "genome": { + "type": "string", + "description": "Name of iGenomes reference.", + "fa_icon": "fas fa-book", + "help_text": "If using a reference genome configured in the pipeline using iGenomes, use this parameter to give the ID for the reference. This is then used to build the full paths for all required reference genome files e.g. `--genome GRCh38`.\n\nSee the [nf-core website docs](https://nf-co.re/usage/reference_genomes) for more details." + }, + "fasta": { + "type": "string", + "fa_icon": "fas fa-font", + "description": "Path to FASTA genome file.", + "help_text": "If you have no genome reference available, the pipeline can build one using a FASTA file. This requires additional time and resources, so it's better to use a pre-build index if possible." + }, + "igenomes_base": { + "type": "string", + "description": "Directory / URL base for iGenomes references.", + "default": "s3://ngi-igenomes/igenomes/", + "fa_icon": "fas fa-cloud-download-alt", + "hidden": true + }, + "igenomes_ignore": { + "type": "boolean", + "description": "Do not load the iGenomes reference config.", + "fa_icon": "fas fa-ban", + "hidden": true, + "help_text": "Do not load `igenomes.config` when running the pipeline. You may choose this option if you observe clashes between custom parameters and those supplied in `igenomes.config`." + } + } + }, "generic_options": { "title": "Generic options", "type": "object", @@ -71,6 +111,14 @@ "hidden": true, "help_text": "A custom name for the pipeline run. Unlike the core nextflow `-name` option with one hyphen this parameter can be reused multiple times, for example if using `-resume`. Passed through to steps such as MultiQC and used for things like report filenames and titles." }, + "email_on_fail": { + "type": "string", + "description": "Email address for completion summary, only when pipeline fails.", + "fa_icon": "fas fa-exclamation-triangle", + "pattern": "^([a-zA-Z0-9_\\-\\.]+)@([a-zA-Z0-9_\\-\\.]+)\\.([a-zA-Z]{2,5})$", + "hidden": true, + "help_text": "This works exactly as with `--email`, except emails are only sent if the workflow is not successful." + }, "plaintext_email": { "type": "boolean", "description": "Send plain-text email instead of HTML.", @@ -105,14 +153,6 @@ "default": "${params.outdir}/pipeline_info", "fa_icon": "fas fa-cogs", "hidden": true - }, - "igenomes_base": { - "type": "string", - "default": "s3://ngi-igenomes/igenomes/" - }, - "igenomes_ignore": { - "type": "string", - "default": "true" } } }, @@ -197,129 +237,15 @@ "fa_icon": "fas fa-users-cog" } } - }, - "contamination_screening": { - "title": "Contamination Screening", - "type": "object", - "description": "", - "default": "", - "fa_icon": "fas fa-box", - "properties": { - "kraken2db": { - "type": "string", - "fa_icon": "fab fa-gitkraken", - "help_text": "See [Kraken2 homepage](https://benlangmead.github.io/aws-indexes/k2) for download\nlinks. Minikraken2 8GB is a reasonable choice, since we run Kraken here mainly just to check for\nsample purity.", - "description": "Path to Kraken2 database." - } - } - }, - "assembly_parameters": { - "title": "Assembly parameters", - "type": "object", - "description": "Parameters for the assembly", - "default": "", - "fa_icon": "fas fa-puzzle-piece", - "properties": { - "assembler": { - "type": "string", - "default": "unicycler", - "fa_icon": "fas fa-puzzle-piece", - "description": "The assembler to use for assembly. Available options are `Unicycler`, `Canu`, `Miniasm`. The latter two are only available for long-read data, whereas Unicycler can be used for short or hybrid assembly projects." - }, - "assembly_type": { - "type": "string", - "default": "short", - "fa_icon": "fas fa-fingerprint", - "help_text": "This adjusts the type of assembly done with the input data and can be any of `long`, `short` or `hybrid`. Short & Hybrid assembly will always run Unicycler, whereas long-read assembly can be configured separately using the `--assembler` parameter.", - "description": "Which type of assembly to perform." - }, - "unicycler_args": { - "type": "string", - "fa_icon": "fas fa-bicycle", - "description": "Extra arguments for Unicycler", - "help_text": "This advanced option allows you to pass extra arguments to Unicycler (e.g. `\"--mode conservative\"` or `\"--no_correct\"`). For this to work you need to quote the arguments and add at least one space." - }, - "canu_args": { - "type": "string", - "fa_icon": "fas fa-ship", - "description": "This can be used to supply [extra options](https://canu.readthedocs.io/en/latest/quick-start.html) to the Canu assembler. Will be ignored when other assemblers are used." - } - } - }, - "annotation": { - "title": "Annotation", - "type": "object", - "description": "", - "default": "", - "fa_icon": "fas fa-align-left", - "properties": { - "annotation_tool": { - "type": "string", - "default": "prokka", - "description": "The annotation method to annotate the final assembly. Default choice is `prokka`, but the `dfast` tool is also available. For the latter, make sure to create your specific config if you're not happy with the default one provided. See [#dfast_config](#dfastconfig) to find out how." - }, - "prokka_args": { - "type": "string", - "description": "Extra arguments for prokka annotation tool.", - "help_text": "This advanced option allows you to pass extra arguments to Prokka (e.g. `\" --rfam\"` or `\" --genus name\"`). For this to work you need to quote the arguments and add at least one space between the arguments. Example:\n\n```bash\n--prokka_args `--rfam --genus Escherichia Coli`\n```\n" - }, - "dfast_config": { - "type": "string", - "default": "/Users/alexanderpeltzer/IDEA/nf-core/bacass/assets/test_config_dfast.py", - "description": "Specifies a configuration file for the [DFAST](https://github.com/nigyta/dfast_core) annotation method. This can be used instead of PROKKA if required to specify a specific config file for annotation. If you want to know how to create your config file, please refer to the [DFAST](https://github.com/nigyta/dfast_core) readme on how to create one. > The default config is just included for testing, so if you want to annotate using DFAST, you have to create a config!" - } - } - }, - "assembly_polishing": { - "title": "Assembly Polishing", - "type": "object", - "description": "", - "default": "", - "fa_icon": "fas fa-user-astronaut", - "properties": { - "polish_method": { - "type": "string", - "default": "medaka", - "fa_icon": "fas fa-hotdog", - "description": "Which assembly polishing method to use.", - "help_text": "Can be used to define which polishing method is used by default for long reads. Default is `medaka`, available options are `nanopolish` or `medaka`." - } - } - }, - "skipping_options": { - "title": "Skipping Options", - "type": "object", - "description": "", - "default": "", - "fa_icon": "fas fa-forward", - "properties": { - "skip_kraken2": { - "type": "string", - "fa_icon": "fas fa-forward", - "description": "Skip running Kraken2 classifier on reads." - }, - "skip_annotation": { - "type": "string", - "fa_icon": "fas fa-forward", - "description": "Skip annotating the assembly with Prokka /DFAST." - }, - "skip_pycoqc": { - "type": "string", - "fa_icon": "fas fa-forward", - "description": "Skip running `PycoQC` on long read input." - }, - "skip_polish": { - "type": "string", - "fa_icon": "fas fa-forward", - "description": "Skip polishing the long-read assembly with FAST5 input. Will not affect short/hybrid assemblies." - } - } } }, "allOf": [ { "$ref": "#/definitions/input_output_options" }, + { + "$ref": "#/definitions/reference_genome_options" + }, { "$ref": "#/definitions/generic_options" }, @@ -328,21 +254,6 @@ }, { "$ref": "#/definitions/institutional_config_options" - }, - { - "$ref": "#/definitions/contamination_screening" - }, - { - "$ref": "#/definitions/assembly_parameters" - }, - { - "$ref": "#/definitions/annotation" - }, - { - "$ref": "#/definitions/assembly_polishing" - }, - { - "$ref": "#/definitions/skipping_options" } ] -} \ No newline at end of file +} From 2e7540d47217ee7a994684a9a97efe4efb71cfdf Mon Sep 17 00:00:00 2001 From: nf-core-bot Date: Thu, 3 Dec 2020 19:17:30 +0000 Subject: [PATCH 02/81] Template update for nf-core/tools version 1.12.1 --- .github/CONTRIBUTING.md | 79 +++++++++++++++++++++++++-- .github/ISSUE_TEMPLATE/bug_report.md | 14 +++++ .github/PULL_REQUEST_TEMPLATE.md | 14 +++-- .github/markdownlint.yml | 9 ++- Dockerfile | 2 +- README.md | 23 +++++++- assets/nf-core-bacass_logo.png | Bin 12605 -> 107638 bytes conf/igenomes.config | 12 ++-- docs/images/nf-core-bacass_logo.png | Bin 428 -> 107638 bytes main.nf | 2 +- 10 files changed, 137 insertions(+), 18 deletions(-) diff --git a/.github/CONTRIBUTING.md b/.github/CONTRIBUTING.md index 58cb32e4..0a6e2e1c 100644 --- a/.github/CONTRIBUTING.md +++ b/.github/CONTRIBUTING.md @@ -18,8 +18,9 @@ If you'd like to write some code for nf-core/bacass, the standard workflow is as 1. Check that there isn't already an issue about your idea in the [nf-core/bacass issues](https://github.com/nf-core/bacass/issues) to avoid duplicating work * If there isn't one already, please create one so that others know you're working on this 2. [Fork](https://help.github.com/en/github/getting-started-with-github/fork-a-repo) the [nf-core/bacass repository](https://github.com/nf-core/bacass) to your GitHub account -3. Make the necessary changes / additions within your forked repository -4. Submit a Pull Request against the `dev` branch and wait for the code to be reviewed and merged +3. Make the necessary changes / additions within your forked repository following [Pipeline conventions](#pipeline-contribution-conventions) +4. Use `nf-core schema build .` and add any new parameters to the pipeline JSON schema (requires [nf-core tools](https://github.com/nf-core/tools) >= 1.10). +5. Submit a Pull Request against the `dev` branch and wait for the code to be reviewed and merged If you're not used to this workflow with git, you can start with some [docs from GitHub](https://help.github.com/en/github/collaborating-with-issues-and-pull-requests) or even their [excellent `git` resources](https://try.github.io/). @@ -30,14 +31,14 @@ Typically, pull-requests are only fully reviewed when these tests are passing, t There are typically two types of tests that run: -### Lint Tests +### Lint tests `nf-core` has a [set of guidelines](https://nf-co.re/developers/guidelines) which all pipelines must adhere to. To enforce these and ensure that all pipelines stay in sync, we have developed a helper tool which runs checks on the pipeline code. This is in the [nf-core/tools repository](https://github.com/nf-core/tools) and once installed can be run locally with the `nf-core lint ` command. If any failures or warnings are encountered, please follow the listed URL for more documentation. -### Pipeline Tests +### Pipeline tests Each `nf-core` pipeline should be set up with a minimal set of test-data. `GitHub Actions` then runs the pipeline on this data to ensure that it exits successfully. @@ -55,3 +56,73 @@ These tests are run both with the latest available version of `Nextflow` and als ## Getting help For further information/help, please consult the [nf-core/bacass documentation](https://nf-co.re/bacass/usage) and don't hesitate to get in touch on the nf-core Slack [#bacass](https://nfcore.slack.com/channels/bacass) channel ([join our Slack here](https://nf-co.re/join/slack)). + +## Pipeline contribution conventions + +To make the nf-core/bacass code and processing logic more understandable for new contributors and to ensure quality, we semi-standardise the way the code and other contributions are written. + +### Adding a new step + +If you wish to contribute a new step, please use the following coding standards: + +1. Define the corresponding input channel into your new process from the expected previous process channel +2. Write the process block (see below). +3. Define the output channel if needed (see below). +4. Add any new flags/options to `nextflow.config` with a default (see below). +5. Add any new flags/options to `nextflow_schema.json` with help text (with `nf-core schema build .`) +6. Add any new flags/options to the help message (for integer/text parameters, print to help the corresponding `nextflow.config` parameter). +7. Add sanity checks for all relevant parameters. +8. Add any new software to the `scrape_software_versions.py` script in `bin/` and the version command to the `scrape_software_versions` process in `main.nf`. +9. Do local tests that the new code works properly and as expected. +10. Add a new test command in `.github/workflow/ci.yaml`. +11. If applicable add a [MultiQC](https://https://multiqc.info/) module. +12. Update MultiQC config `assets/multiqc_config.yaml` so relevant suffixes, name clean up, General Statistics Table column order, and module figures are in the right order. +13. Optional: Add any descriptions of MultiQC report sections and output files to `docs/output.md`. + +### Default values + +Parameters should be initialised / defined with default values in `nextflow.config` under the `params` scope. + +Once there, use `nf-core schema build .` to add to `nextflow_schema.json`. + +### Default processes resource requirements + +Sensible defaults for process resource requirements (CPUs / memory / time) for a process should be defined in `conf/base.config`. These should generally be specified generic with `withLabel:` selectors so they can be shared across multiple processes/steps of the pipeline. A nf-core standard set of labels that should be followed where possible can be seen in the [nf-core pipeline template](https://github.com/nf-core/tools/blob/master/nf_core/pipeline-template/%7B%7Bcookiecutter.name_noslash%7D%7D/conf/base.config), which has the default process as a single core-process, and then different levels of multi-core configurations for increasingly large memory requirements defined with standardised labels. + +The process resources can be passed on to the tool dynamically within the process with the `${task.cpu}` and `${task.memory}` variables in the `script:` block. + +### Naming schemes + +Please use the following naming schemes, to make it easy to understand what is going where. + +* initial process channel: `ch_output_from_` +* intermediate and terminal channels: `ch__for_` + +### Nextflow version bumping + +If you are using a new feature from core Nextflow, you may bump the minimum required version of nextflow in the pipeline with: `nf-core bump-version --nextflow . [min-nf-version]` + +### Software version reporting + +If you add a new tool to the pipeline, please ensure you add the information of the tool to the `get_software_version` process. + +Add to the script block of the process, something like the following: + +```bash + --version &> v_.txt 2>&1 || true +``` + +or + +```bash + --help | head -n 1 &> v_.txt 2>&1 || true +``` + +You then need to edit the script `bin/scrape_software_versions.py` to: + +1. Add a Python regex for your tool's `--version` output (as in stored in the `v_.txt` file), to ensure the version is reported as a `v` and the version number e.g. `v2.1.1` +2. Add a HTML entry to the `OrderedDict` for formatting in MultiQC. + +### Images and figures + +For overview images and other documents we follow the nf-core [style guidelines and examples](https://nf-co.re/developers/design_guidelines). diff --git a/.github/ISSUE_TEMPLATE/bug_report.md b/.github/ISSUE_TEMPLATE/bug_report.md index 5a3ee9d4..47667bb5 100644 --- a/.github/ISSUE_TEMPLATE/bug_report.md +++ b/.github/ISSUE_TEMPLATE/bug_report.md @@ -13,6 +13,13 @@ Thanks for telling us about a problem with the pipeline. Please delete this text and anything that's not relevant from the template below: --> +## Check Documentation + +I have checked the following places for your error: + +- [ ] [nf-core website: troubleshooting](https://nf-co.re/usage/troubleshooting) +- [ ] [nf-core/bacass pipeline documentation](https://nf-co.re/nf-core/bacass/usage) + ## Description of the bug @@ -28,6 +35,13 @@ Steps to reproduce the behaviour: +## Log files + +Have you provided the following extra information/files: + +- [ ] The command used to run the pipeline +- [ ] The `.nextflow.log` file + ## System - Hardware: diff --git a/.github/PULL_REQUEST_TEMPLATE.md b/.github/PULL_REQUEST_TEMPLATE.md index 24d60366..b9c264cb 100644 --- a/.github/PULL_REQUEST_TEMPLATE.md +++ b/.github/PULL_REQUEST_TEMPLATE.md @@ -13,8 +13,14 @@ Learn more about contributing: [CONTRIBUTING.md](https://github.com/nf-core/baca ## PR checklist -- [ ] This comment contains a description of changes (with reason) -- [ ] `CHANGELOG.md` is updated +- [ ] This comment contains a description of changes (with reason). - [ ] If you've fixed a bug or added code that should be tested, add tests! -- [ ] Documentation in `docs` is updated -- [ ] If necessary, also make a PR on the [nf-core/bacass branch on the nf-core/test-datasets repo](https://github.com/nf-core/test-datasets/pull/new/nf-core/bacass) + - [ ] If you've added a new tool - add to the software_versions process and a regex to `scrape_software_versions.py` + - [ ] If you've added a new tool - have you followed the pipeline conventions in the [contribution docs](https://github.com/nf-core/bacass/tree/master/.github/CONTRIBUTING.md) + - [ ] If necessary, also make a PR on the nf-core/bacass _branch_ on the [nf-core/test-datasets](https://github.com/nf-core/test-datasets) repository. +- [ ] Make sure your code lints (`nf-core lint .`). +- [ ] Ensure the test suite passes (`nextflow run . -profile test,docker`). +- [ ] Usage Documentation in `docs/usage.md` is updated. +- [ ] Output Documentation in `docs/output.md` is updated. +- [ ] `CHANGELOG.md` is updated. +- [ ] `README.md` is updated (including new tool citations and authors/contributors). diff --git a/.github/markdownlint.yml b/.github/markdownlint.yml index 96b12a70..8d7eb53b 100644 --- a/.github/markdownlint.yml +++ b/.github/markdownlint.yml @@ -1,5 +1,12 @@ # Markdownlint configuration file -default: true, +default: true line-length: false no-duplicate-header: siblings_only: true +no-inline-html: + allowed_elements: + - img + - p + - kbd + - details + - summary diff --git a/Dockerfile b/Dockerfile index 88f669a0..89018ab3 100644 --- a/Dockerfile +++ b/Dockerfile @@ -1,4 +1,4 @@ -FROM nfcore/base:1.12 +FROM nfcore/base:1.12.1 LABEL authors="Andreas Wilm, Alexander Peltzer" \ description="Docker image containing all software requirements for the nf-core/bacass pipeline" diff --git a/README.md b/README.md index aed58100..b0835331 100644 --- a/README.md +++ b/README.md @@ -12,6 +12,9 @@ ## Introduction + +**nf-core/bacass** is a bioinformatics best-practise analysis pipeline for + The pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool to run tasks across multiple compute infrastructures in a very portable manner. It comes with docker containers making installation trivial and results highly reproducible. ## Quick Start @@ -38,6 +41,15 @@ The pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool See [usage docs](https://nf-co.re/bacass/usage) for all of the available options when running the pipeline. +## Pipeline Summary + +By default, the pipeline currently performs the following: + + + +* Sequencing quality control (`FastQC`) +* Overall pipeline run summaries (`MultiQC`) + ## Documentation The nf-core/bacass pipeline comes with documentation about the pipeline: [usage](https://nf-co.re/bacass/usage) and [output](https://nf-co.re/bacass/output). @@ -48,13 +60,18 @@ The nf-core/bacass pipeline comes with documentation about the pipeline: [usage] nf-core/bacass was originally written by Andreas Wilm, Alexander Peltzer. +We thank the following people for their extensive assistance in the development +of this pipeline: + + + ## Contributions and Support If you would like to contribute to this pipeline, please see the [contributing guidelines](.github/CONTRIBUTING.md). For further information or help, don't hesitate to get in touch on the [Slack `#bacass` channel](https://nfcore.slack.com/channels/bacass) (you can join with [this invite](https://nf-co.re/join/slack)). -## Citation +## Citations @@ -67,3 +84,7 @@ You can cite the `nf-core` publication as follows: > > _Nat Biotechnol._ 2020 Feb 13. doi: [10.1038/s41587-020-0439-x](https://dx.doi.org/10.1038/s41587-020-0439-x). > ReadCube: [Full Access Link](https://rdcu.be/b1GjZ) + +In addition, references of tools and data used in this pipeline are as follows: + + diff --git a/assets/nf-core-bacass_logo.png b/assets/nf-core-bacass_logo.png index 9cfbc372f820ce36db669a78b5980948e3214194..37e3ec62a04a57ea7e86e5a2e4dcec1b220cb034 100644 GIT binary patch literal 107638 zcmeFXhgZ{C_dOiI5tXK*BGP7%BGP+J7)KNX0tzY}M4I#_EtKG(AW|X;y{a@30#ZT` zMMfZ0r6lwsozP1Hge30=N9Xzd5AXM`Wm&qox%ZrX_SyT~MBUTZW;=7`3oJc83c=9X{HRe8B&y@}tlE6%`$=cv5>836b}!x*FMFwuPp85=C9ZN__~I|+w-J&> 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J-waT~81OO72jb}RoLJM9sUyE8jGGwP=P zT({r52Hx8DE$sF$+szslcT#Se_EpNdtuM&KawTQ+v8aUV!(c1b6Y4w!6D(Cvt4H`E zKADbS@9>1i;=z)V7g#7c)78Q2R21&3KiPRN)L6AW!IagA!AuZn7(F8!n;^O(3&GEL z`IdZS!SoYp3ZUbR4QN~-hc4sVpItBmfoJG&#Lk2yM)p}|n3k}!gi}7^1bED6U@;Ic zl^ka$&sv*cyhCf#Eg<}pQxJ33KjQ@CIv?|QCJpD_6tkR+I~LC|e?kXMA=kc(1G!N4 VJvv>8IrcTl)qtG)%H@^D#V=*Sk$eCE diff --git a/main.nf b/main.nf index 10359131..106e395f 100644 --- a/main.nf +++ b/main.nf @@ -336,7 +336,7 @@ workflow.onComplete { def email_html = html_template.toString() // Render the sendmail template - def smail_fields = [ email: email_address, subject: subject, email_txt: email_txt, email_html: email_html, baseDir: "$projectDir", mqcFile: mqc_report, mqcMaxSize: params.max_multiqc_email_size.toBytes() ] + def smail_fields = [ email: email_address, subject: subject, email_txt: email_txt, email_html: email_html, projectDir: "$projectDir", mqcFile: mqc_report, mqcMaxSize: params.max_multiqc_email_size.toBytes() ] def sf = new File("$projectDir/assets/sendmail_template.txt") def sendmail_template = engine.createTemplate(sf).make(smail_fields) def sendmail_html = sendmail_template.toString() From 225a827dbe98b0b267afea6885f7cdc237039346 Mon Sep 17 00:00:00 2001 From: nf-core-bot Date: Thu, 18 Mar 2021 12:05:50 +0000 Subject: [PATCH 03/81] Template update for nf-core/tools version 1.13 --- .github/.dockstore.yml | 1 + .github/CONTRIBUTING.md | 4 +- .github/ISSUE_TEMPLATE/bug_report.md | 2 +- .github/ISSUE_TEMPLATE/feature_request.md | 2 +- .github/PULL_REQUEST_TEMPLATE.md | 6 +- .github/workflows/awsfulltest.yml | 17 +- .github/workflows/awstest.yml | 17 +- .github/workflows/branch.yml | 7 +- .github/workflows/linting.yml | 2 +- CODE_OF_CONDUCT.md | 117 ++++- Dockerfile | 6 +- README.md | 7 +- assets/email_template.html | 1 - assets/nf-core-bacass_logo.png | Bin 107638 -> 18186 bytes bin/markdown_to_html.py | 0 bin/scrape_software_versions.py | 0 conf/base.config | 2 +- conf/test.config | 2 + conf/test_full.config | 2 + docs/images/nf-core-bacass_logo.png | Bin 107638 -> 34789 bytes docs/output.md | 4 - docs/usage.md | 12 +- lib/Headers.groovy | 43 ++ lib/NfcoreSchema.groovy | 571 ++++++++++++++++++++++ lib/nfcore_external_java_deps.jar | Bin 0 -> 2291171 bytes main.nf | 143 ++---- nextflow.config | 42 +- nextflow_schema.json | 27 +- 28 files changed, 866 insertions(+), 171 deletions(-) mode change 100755 => 100644 bin/markdown_to_html.py mode change 100755 => 100644 bin/scrape_software_versions.py create mode 100644 lib/Headers.groovy create mode 100644 lib/NfcoreSchema.groovy create mode 100644 lib/nfcore_external_java_deps.jar diff --git a/.github/.dockstore.yml b/.github/.dockstore.yml index 030138a0..191fabd2 100644 --- a/.github/.dockstore.yml +++ b/.github/.dockstore.yml @@ -3,3 +3,4 @@ version: 1.2 workflows: - subclass: nfl primaryDescriptorPath: /nextflow.config + publish: True diff --git a/.github/CONTRIBUTING.md b/.github/CONTRIBUTING.md index 0a6e2e1c..73d2e7b0 100644 --- a/.github/CONTRIBUTING.md +++ b/.github/CONTRIBUTING.md @@ -69,7 +69,7 @@ If you wish to contribute a new step, please use the following coding standards: 2. Write the process block (see below). 3. Define the output channel if needed (see below). 4. Add any new flags/options to `nextflow.config` with a default (see below). -5. Add any new flags/options to `nextflow_schema.json` with help text (with `nf-core schema build .`) +5. Add any new flags/options to `nextflow_schema.json` with help text (with `nf-core schema build .`). 6. Add any new flags/options to the help message (for integer/text parameters, print to help the corresponding `nextflow.config` parameter). 7. Add sanity checks for all relevant parameters. 8. Add any new software to the `scrape_software_versions.py` script in `bin/` and the version command to the `scrape_software_versions` process in `main.nf`. @@ -87,7 +87,7 @@ Once there, use `nf-core schema build .` to add to `nextflow_schema.json`. ### Default processes resource requirements -Sensible defaults for process resource requirements (CPUs / memory / time) for a process should be defined in `conf/base.config`. These should generally be specified generic with `withLabel:` selectors so they can be shared across multiple processes/steps of the pipeline. A nf-core standard set of labels that should be followed where possible can be seen in the [nf-core pipeline template](https://github.com/nf-core/tools/blob/master/nf_core/pipeline-template/%7B%7Bcookiecutter.name_noslash%7D%7D/conf/base.config), which has the default process as a single core-process, and then different levels of multi-core configurations for increasingly large memory requirements defined with standardised labels. +Sensible defaults for process resource requirements (CPUs / memory / time) for a process should be defined in `conf/base.config`. These should generally be specified generic with `withLabel:` selectors so they can be shared across multiple processes/steps of the pipeline. A nf-core standard set of labels that should be followed where possible can be seen in the [nf-core pipeline template](https://github.com/nf-core/tools/blob/master/nf_core/pipeline-template/conf/base.config), which has the default process as a single core-process, and then different levels of multi-core configurations for increasingly large memory requirements defined with standardised labels. The process resources can be passed on to the tool dynamically within the process with the `${task.cpu}` and `${task.memory}` variables in the `script:` block. diff --git a/.github/ISSUE_TEMPLATE/bug_report.md b/.github/ISSUE_TEMPLATE/bug_report.md index 47667bb5..d6c86ac5 100644 --- a/.github/ISSUE_TEMPLATE/bug_report.md +++ b/.github/ISSUE_TEMPLATE/bug_report.md @@ -55,7 +55,7 @@ Have you provided the following extra information/files: ## Container engine -- Engine: +- Engine: - version: - Image tag: diff --git a/.github/ISSUE_TEMPLATE/feature_request.md b/.github/ISSUE_TEMPLATE/feature_request.md index 327248ec..29121dbd 100644 --- a/.github/ISSUE_TEMPLATE/feature_request.md +++ b/.github/ISSUE_TEMPLATE/feature_request.md @@ -1,6 +1,6 @@ --- name: Feature request -about: Suggest an idea for the nf-core website +about: Suggest an idea for the nf-core/bacass pipeline labels: enhancement --- diff --git a/.github/PULL_REQUEST_TEMPLATE.md b/.github/PULL_REQUEST_TEMPLATE.md index b9c264cb..e8ed6a41 100644 --- a/.github/PULL_REQUEST_TEMPLATE.md +++ b/.github/PULL_REQUEST_TEMPLATE.md @@ -15,9 +15,9 @@ Learn more about contributing: [CONTRIBUTING.md](https://github.com/nf-core/baca - [ ] This comment contains a description of changes (with reason). - [ ] If you've fixed a bug or added code that should be tested, add tests! - - [ ] If you've added a new tool - add to the software_versions process and a regex to `scrape_software_versions.py` - - [ ] If you've added a new tool - have you followed the pipeline conventions in the [contribution docs](https://github.com/nf-core/bacass/tree/master/.github/CONTRIBUTING.md) - - [ ] If necessary, also make a PR on the nf-core/bacass _branch_ on the [nf-core/test-datasets](https://github.com/nf-core/test-datasets) repository. + - [ ] If you've added a new tool - add to the software_versions process and a regex to `scrape_software_versions.py` + - [ ] If you've added a new tool - have you followed the pipeline conventions in the [contribution docs](https://github.com/nf-core/bacass/tree/master/.github/CONTRIBUTING.md) + - [ ] If necessary, also make a PR on the nf-core/bacass _branch_ on the [nf-core/test-datasets](https://github.com/nf-core/test-datasets) repository. - [ ] Make sure your code lints (`nf-core lint .`). - [ ] Ensure the test suite passes (`nextflow run . -profile test,docker`). - [ ] Usage Documentation in `docs/usage.md` is updated. diff --git a/.github/workflows/awsfulltest.yml b/.github/workflows/awsfulltest.yml index b2edaf4a..3314a099 100644 --- a/.github/workflows/awsfulltest.yml +++ b/.github/workflows/awsfulltest.yml @@ -9,6 +9,16 @@ on: types: [completed] workflow_dispatch: + +env: + AWS_ACCESS_KEY_ID: ${{ secrets.AWS_ACCESS_KEY_ID }} + AWS_SECRET_ACCESS_KEY: ${{ secrets.AWS_SECRET_ACCESS_KEY }} + TOWER_ACCESS_TOKEN: ${{ secrets.AWS_TOWER_TOKEN }} + AWS_JOB_DEFINITION: ${{ secrets.AWS_JOB_DEFINITION }} + AWS_JOB_QUEUE: ${{ secrets.AWS_JOB_QUEUE }} + AWS_S3_BUCKET: ${{ secrets.AWS_S3_BUCKET }} + + jobs: run-awstest: name: Run AWS full tests @@ -27,13 +37,6 @@ jobs: # Add full size test data (but still relatively small datasets for few samples) # on the `test_full.config` test runs with only one set of parameters # Then specify `-profile test_full` instead of `-profile test` on the AWS batch command - env: - AWS_ACCESS_KEY_ID: ${{ secrets.AWS_ACCESS_KEY_ID }} - AWS_SECRET_ACCESS_KEY: ${{ secrets.AWS_SECRET_ACCESS_KEY }} - TOWER_ACCESS_TOKEN: ${{ secrets.AWS_TOWER_TOKEN }} - AWS_JOB_DEFINITION: ${{ secrets.AWS_JOB_DEFINITION }} - AWS_JOB_QUEUE: ${{ secrets.AWS_JOB_QUEUE }} - AWS_S3_BUCKET: ${{ secrets.AWS_S3_BUCKET }} run: | aws batch submit-job \ --region eu-west-1 \ diff --git a/.github/workflows/awstest.yml b/.github/workflows/awstest.yml index 8e5bdbb0..c61b647f 100644 --- a/.github/workflows/awstest.yml +++ b/.github/workflows/awstest.yml @@ -6,6 +6,16 @@ name: nf-core AWS test on: workflow_dispatch: + +env: + AWS_ACCESS_KEY_ID: ${{ secrets.AWS_ACCESS_KEY_ID }} + AWS_SECRET_ACCESS_KEY: ${{ secrets.AWS_SECRET_ACCESS_KEY }} + TOWER_ACCESS_TOKEN: ${{ secrets.AWS_TOWER_TOKEN }} + AWS_JOB_DEFINITION: ${{ secrets.AWS_JOB_DEFINITION }} + AWS_JOB_QUEUE: ${{ secrets.AWS_JOB_QUEUE }} + AWS_S3_BUCKET: ${{ secrets.AWS_S3_BUCKET }} + + jobs: run-awstest: name: Run AWS tests @@ -23,13 +33,6 @@ jobs: # TODO nf-core: You can customise CI pipeline run tests as required # For example: adding multiple test runs with different parameters # Remember that you can parallelise this by using strategy.matrix - env: - AWS_ACCESS_KEY_ID: ${{ secrets.AWS_ACCESS_KEY_ID }} - AWS_SECRET_ACCESS_KEY: ${{ secrets.AWS_SECRET_ACCESS_KEY }} - TOWER_ACCESS_TOKEN: ${{ secrets.AWS_TOWER_TOKEN }} - AWS_JOB_DEFINITION: ${{ secrets.AWS_JOB_DEFINITION }} - AWS_JOB_QUEUE: ${{ secrets.AWS_JOB_QUEUE }} - AWS_S3_BUCKET: ${{ secrets.AWS_S3_BUCKET }} run: | aws batch submit-job \ --region eu-west-1 \ diff --git a/.github/workflows/branch.yml b/.github/workflows/branch.yml index 6b74a395..3f0e2153 100644 --- a/.github/workflows/branch.yml +++ b/.github/workflows/branch.yml @@ -13,7 +13,7 @@ jobs: - name: Check PRs if: github.repository == 'nf-core/bacass' run: | - { [[ ${{github.event.pull_request.head.repo.full_name}} == nf-core/bacass ]] && [[ $GITHUB_HEAD_REF = "dev" ]]; } || [[ $GITHUB_HEAD_REF == "patch" ]] + { [[ ${{github.event.pull_request.head.repo.full_name }} == nf-core/bacass ]] && [[ $GITHUB_HEAD_REF = "dev" ]]; } || [[ $GITHUB_HEAD_REF == "patch" ]] # If the above check failed, post a comment on the PR explaining the failure @@ -25,11 +25,12 @@ jobs: message: | Hi @${{ github.event.pull_request.user.login }}, - It looks like this pull-request is has been made against the ${{github.event.pull_request.head.repo.full_name}} `master` branch. + It looks like this pull-request is has been made against the ${{github.event.pull_request.base.repo.full_name }} `master` branch. The `master` branch on nf-core repositories should always contain code from the latest release. - Because of this, PRs to `master` are only allowed if they come from the ${{github.event.pull_request.head.repo.full_name}} `dev` branch. + Because of this, PRs to `master` are only allowed if they come from the ${{github.event.pull_request.base.repo.full_name }} `dev` branch. You do not need to close this PR, you can change the target branch to `dev` by clicking the _"Edit"_ button at the top of this page. + Note that even after this, the test will continue to show as failing until you push a new commit. Thanks again for your contribution! repo-token: ${{ secrets.GITHUB_TOKEN }} diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml index bef81e61..58e281df 100644 --- a/.github/workflows/linting.yml +++ b/.github/workflows/linting.yml @@ -69,7 +69,7 @@ jobs: if: ${{ always() }} uses: actions/upload-artifact@v2 with: - name: linting-log-file + name: linting-logs path: | lint_log.txt lint_results.md diff --git a/CODE_OF_CONDUCT.md b/CODE_OF_CONDUCT.md index 405fb1bf..f4fd052f 100644 --- a/CODE_OF_CONDUCT.md +++ b/CODE_OF_CONDUCT.md @@ -1,46 +1,111 @@ -# Contributor Covenant Code of Conduct +# Code of Conduct at nf-core (v1.0) ## Our Pledge -In the interest of fostering an open and welcoming environment, we as contributors and maintainers pledge to making participation in our project and our community a harassment-free experience for everyone, regardless of age, body size, disability, ethnicity, gender identity and expression, level of experience, nationality, personal appearance, race, religion, or sexual identity and orientation. +In the interest of fostering an open, collaborative, and welcoming environment, we as contributors and maintainers of nf-core, pledge to making participation in our projects and community a harassment-free experience for everyone, regardless of: -## Our Standards +- Age +- Body size +- Familial status +- Gender identity and expression +- Geographical location +- Level of experience +- Nationality and national origins +- Native language +- Physical and neurological ability +- Race or ethnicity +- Religion +- Sexual identity and orientation +- Socioeconomic status -Examples of behavior that contributes to creating a positive environment include: +Please note that the list above is alphabetised and is therefore not ranked in any order of preference or importance. -* Using welcoming and inclusive language -* Being respectful of differing viewpoints and experiences -* Gracefully accepting constructive criticism -* Focusing on what is best for the community -* Showing empathy towards other community members +## Preamble -Examples of unacceptable behavior by participants include: +> Note: This Code of Conduct (CoC) has been drafted by the nf-core Safety Officer and been edited after input from members of the nf-core team and others. "We", in this document, refers to the Safety Officer and members of the nf-core core team, both of whom are deemed to be members of the nf-core community and are therefore required to abide by this Code of Conduct. This document will amended periodically to keep it up-to-date, and in case of any dispute, the most current version will apply. -* The use of sexualized language or imagery and unwelcome sexual attention or advances -* Trolling, insulting/derogatory comments, and personal or political attacks -* Public or private harassment -* Publishing others' private information, such as a physical or electronic address, without explicit permission -* Other conduct which could reasonably be considered inappropriate in a professional setting +An up-to-date list of members of the nf-core core team can be found [here](https://nf-co.re/about). Our current safety officer is Renuka Kudva. + +nf-core is a young and growing community that welcomes contributions from anyone with a shared vision for [Open Science Policies](https://www.fosteropenscience.eu/taxonomy/term/8). Open science policies encompass inclusive behaviours and we strive to build and maintain a safe and inclusive environment for all individuals. + +We have therefore adopted this code of conduct (CoC), which we require all members of our community and attendees in nf-core events to adhere to in all our workspaces at all times. Workspaces include but are not limited to Slack, meetings on Zoom, Jitsi, YouTube live etc. + +Our CoC will be strictly enforced and the nf-core team reserve the right to exclude participants who do not comply with our guidelines from our workspaces and future nf-core activities. + +We ask all members of our community to help maintain a supportive and productive workspace and to avoid behaviours that can make individuals feel unsafe or unwelcome. Please help us maintain and uphold this CoC. + +Questions, concerns or ideas on what we can include? Contact safety [at] nf-co [dot] re ## Our Responsibilities -Project maintainers are responsible for clarifying the standards of acceptable behavior and are expected to take appropriate and fair corrective action in response to any instances of unacceptable behavior. +The safety officer is responsible for clarifying the standards of acceptable behavior and are expected to take appropriate and fair corrective action in response to any instances of unacceptable behaviour. + +The safety officer in consultation with the nf-core core team have the right and responsibility to remove, edit, or reject comments, commits, code, wiki edits, issues, and other contributions that are not aligned to this Code of Conduct, or to ban temporarily or permanently any contributor for other behaviors that they deem inappropriate, threatening, offensive, or harmful. + +Members of the core team or the safety officer who violate the CoC will be required to recuse themselves pending investigation. They will not have access to any reports of the violations and be subject to the same actions as others in violation of the CoC. + +## When are where does this Code of Conduct apply? + +Participation in the nf-core community is contingent on following these guidelines in all our workspaces and events. This includes but is not limited to the following listed alphabetically and therefore in no order of preference: + +- Communicating with an official project email address. +- Communicating with community members within the nf-core Slack channel. +- Participating in hackathons organised by nf-core (both online and in-person events). +- Participating in collaborative work on GitHub, Google Suite, community calls, mentorship meetings, email correspondence. +- Participating in workshops, training, and seminar series organised by nf-core (both online and in-person events). This applies to events hosted on web-based platforms such as Zoom, Jitsi, YouTube live etc. +- Representing nf-core on social media. This includes both official and personal accounts. + +## nf-core cares 😊 + +nf-core's CoC and expectations of respectful behaviours for all participants (including organisers and the nf-core team) include but are not limited to the following (listed in alphabetical order): + +- Ask for consent before sharing another community member’s personal information (including photographs) on social media. +- Be respectful of differing viewpoints and experiences. We are all here to learn from one another and a difference in opinion can present a good learning opportunity. +- Celebrate your accomplishments at events! (Get creative with your use of emojis 🎉 🥳 💯 🙌 !) +- Demonstrate empathy towards other community members. (We don’t all have the same amount of time to dedicate to nf-core. If tasks are pending, don’t hesitate to gently remind members of your team. If you are leading a task, ask for help if you feel overwhelmed.) +- Engage with and enquire after others. (This is especially important given the geographically remote nature of the nf-core community, so let’s do this the best we can) +- Focus on what is best for the team and the community. (When in doubt, ask) +- Graciously accept constructive criticism, yet be unafraid to question, deliberate, and learn. +- Introduce yourself to members of the community. (We’ve all been outsiders and we know that talking to strangers can be hard for some, but remember we’re interested in getting to know you and your visions for open science!) +- Show appreciation and **provide clear feedback**. (This is especially important because we don’t see each other in person and it can be harder to interpret subtleties. Also remember that not everyone understands a certain language to the same extent as you do, so **be clear in your communications to be kind.**) +- Take breaks when you feel like you need them. +- Using welcoming and inclusive language. (Participants are encouraged to display their chosen pronouns on Zoom or in communication on Slack.) + +## nf-core frowns on 😕 + +The following behaviours from any participants within the nf-core community (including the organisers) will be considered unacceptable under this code of conduct. Engaging or advocating for any of the following could result in expulsion from nf-core workspaces. + +- Deliberate intimidation, stalking or following and sustained disruption of communication among participants of the community. This includes hijacking shared screens through actions such as using the annotate tool in conferencing software such as Zoom. +- “Doxing” i.e. posting (or threatening to post) another person’s personal identifying information online. +- Spamming or trolling of individuals on social media. +- Use of sexual or discriminatory imagery, comments, or jokes and unwelcome sexual attention. +- Verbal and text comments that reinforce social structures of domination related to gender, gender identity and expression, sexual orientation, ability, physical appearance, body size, race, age, religion or work experience. + +### Online Trolling + +The majority of nf-core interactions and events are held online. Unfortunately, holding events online comes with the added issue of online trolling. This is unacceptable, reports of such behaviour will be taken very seriously, and perpetrators will be excluded from activities immediately. + +All community members are required to ask members of the group they are working within for explicit consent prior to taking screenshots of individuals during video calls. + +## Procedures for Reporting CoC violations -Project maintainers have the right and responsibility to remove, edit, or reject comments, commits, code, wiki edits, issues, and other contributions that are not aligned to this Code of Conduct, or to ban temporarily or permanently any contributor for other behaviors that they deem inappropriate, threatening, offensive, or harmful. +If someone makes you feel uncomfortable through their behaviours or actions, report it as soon as possible. -## Scope +You can reach out to members of the [nf-core core team](https://nf-co.re/about) and they will forward your concerns to the safety officer(s). -This Code of Conduct applies both within project spaces and in public spaces when an individual is representing the project or its community. Examples of representing a project or community include using an official project e-mail address, posting via an official social media account, or acting as an appointed representative at an online or offline event. Representation of a project may be further defined and clarified by project maintainers. +Issues directly concerning members of the core team will be dealt with by other members of the core team and the safety manager, and possible conflicts of interest will be taken into account. nf-core is also in discussions about having an ombudsperson, and details will be shared in due course. -## Enforcement +All reports will be handled with utmost discretion and confidentially. -Instances of abusive, harassing, or otherwise unacceptable behavior may be reported by contacting the project team on [Slack](https://nf-co.re/join/slack). The project team will review and investigate all complaints, and will respond in a way that it deems appropriate to the circumstances. The project team is obligated to maintain confidentiality with regard to the reporter of an incident. Further details of specific enforcement policies may be posted separately. +## Attribution and Acknowledgements -Project maintainers who do not follow or enforce the Code of Conduct in good faith may face temporary or permanent repercussions as determined by other members of the project's leadership. +- The [Contributor Covenant, version 1.4](http://contributor-covenant.org/version/1/4) +- The [OpenCon 2017 Code of Conduct](http://www.opencon2017.org/code_of_conduct) (CC BY 4.0 OpenCon organisers, SPARC and Right to Research Coalition) +- The [eLife innovation sprint 2020 Code of Conduct](https://sprint.elifesciences.org/code-of-conduct/) +- The [Mozilla Community Participation Guidelines v3.1](https://www.mozilla.org/en-US/about/governance/policies/participation/) (version 3.1, CC BY-SA 3.0 Mozilla) -## Attribution +## Changelog -This Code of Conduct is adapted from the [Contributor Covenant][homepage], version 1.4, available at [https://www.contributor-covenant.org/version/1/4/code-of-conduct/][version] +### v1.0 - March 12th, 2021 -[homepage]: https://contributor-covenant.org -[version]: https://www.contributor-covenant.org/version/1/4/code-of-conduct/ +- Complete rewrite from original [Contributor Covenant](http://contributor-covenant.org/) CoC. diff --git a/Dockerfile b/Dockerfile index 89018ab3..ea598a79 100644 --- a/Dockerfile +++ b/Dockerfile @@ -1,4 +1,4 @@ -FROM nfcore/base:1.12.1 +FROM nfcore/base:1.13 LABEL authors="Andreas Wilm, Alexander Peltzer" \ description="Docker image containing all software requirements for the nf-core/bacass pipeline" @@ -11,7 +11,3 @@ ENV PATH /opt/conda/envs/nf-core-bacass-1.1.1/bin:$PATH # Dump the details of the installed packages to a file for posterity RUN conda env export --name nf-core-bacass-1.1.1 > nf-core-bacass-1.1.1.yml - -# Instruct R processes to use these empty files instead of clashing with a local version -RUN touch .Rprofile -RUN touch .Renviron diff --git a/README.md b/README.md index b0835331..3b807be6 100644 --- a/README.md +++ b/README.md @@ -21,12 +21,12 @@ The pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool 1. Install [`nextflow`](https://nf-co.re/usage/installation) -2. Install any of [`Docker`](https://docs.docker.com/engine/installation/), [`Singularity`](https://www.sylabs.io/guides/3.0/user-guide/) or [`Podman`](https://podman.io/) for full pipeline reproducibility _(please only use [`Conda`](https://conda.io/miniconda.html) as a last resort; see [docs](https://nf-co.re/usage/configuration#basic-configuration-profiles))_ +2. Install any of [`Docker`](https://docs.docker.com/engine/installation/), [`Singularity`](https://www.sylabs.io/guides/3.0/user-guide/), [`Podman`](https://podman.io/), [`Shifter`](https://nersc.gitlab.io/development/shifter/how-to-use/) or [`Charliecloud`](https://hpc.github.io/charliecloud/) for full pipeline reproducibility _(please only use [`Conda`](https://conda.io/miniconda.html) as a last resort; see [docs](https://nf-co.re/usage/configuration#basic-configuration-profiles))_ 3. Download the pipeline and test it on a minimal dataset with a single command: ```bash - nextflow run nf-core/bacass -profile test, + nextflow run nf-core/bacass -profile test, ``` > Please check [nf-core/configs](https://github.com/nf-core/configs#documentation) to see if a custom config file to run nf-core pipelines already exists for your Institute. If so, you can simply use `-profile ` in your command. This will enable either `docker` or `singularity` and set the appropriate execution settings for your local compute environment. @@ -36,7 +36,7 @@ The pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool ```bash - nextflow run nf-core/bacass -profile --input '*_R{1,2}.fastq.gz' --genome GRCh37 + nextflow run nf-core/bacass -profile --input '*_R{1,2}.fastq.gz' --genome GRCh37 ``` See [usage docs](https://nf-co.re/bacass/usage) for all of the available options when running the pipeline. @@ -83,7 +83,6 @@ You can cite the `nf-core` publication as follows: > Philip Ewels, Alexander Peltzer, Sven Fillinger, Harshil Patel, Johannes Alneberg, Andreas Wilm, Maxime Ulysse Garcia, Paolo Di Tommaso & Sven Nahnsen. > > _Nat Biotechnol._ 2020 Feb 13. doi: [10.1038/s41587-020-0439-x](https://dx.doi.org/10.1038/s41587-020-0439-x). -> ReadCube: [Full Access Link](https://rdcu.be/b1GjZ) In addition, references of tools and data used in this pipeline are as follows: diff --git 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z`+ai{bQf1Cm@`50_UwCFy@BFTYBrq}ad# zhYmO*^KvMa{-G6QXJ(gP)Ze7Kerr;mYpS3E{KM`4{`>zg11BymC _Documentation of pipeline parameters is generated automatically from the pipeline schema and can no longer be found in markdown files._ - ## Introduction This document describes the output produced by the pipeline. Most of the plots are taken from the MultiQC report, which summarises results at the end of the pipeline. diff --git a/docs/usage.md b/docs/usage.md index 9cfcb7d2..38d77cb0 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -51,7 +51,7 @@ This version number will be logged in reports when you run the pipeline, so that Use this parameter to choose a configuration profile. Profiles can give configuration presets for different compute environments. -Several generic profiles are bundled with the pipeline which instruct the pipeline to use software packaged using different methods (Docker, Singularity, Podman, Conda) - see below. +Several generic profiles are bundled with the pipeline which instruct the pipeline to use software packaged using different methods (Docker, Singularity, Podman, Shifter, Charliecloud, Conda) - see below. > We highly recommend the use of Docker or Singularity containers for full pipeline reproducibility, however when this is not possible, Conda is also supported. @@ -71,8 +71,14 @@ If `-profile` is not specified, the pipeline will run locally and expect all sof * `podman` * A generic configuration profile to be used with [Podman](https://podman.io/) * Pulls software from Docker Hub: [`nfcore/bacass`](https://hub.docker.com/r/nfcore/bacass/) +* `shifter` + * A generic configuration profile to be used with [Shifter](https://nersc.gitlab.io/development/shifter/how-to-use/) + * Pulls software from Docker Hub: [`nfcore/bacass`](https://hub.docker.com/r/nfcore/bacass/) +* `charliecloud` + * A generic configuration profile to be used with [Charliecloud](https://hpc.github.io/charliecloud/) + * Pulls software from Docker Hub: [`nfcore/bacass`](https://hub.docker.com/r/nfcore/bacass/) * `conda` - * Please only use Conda as a last resort i.e. when it's not possible to run the pipeline with Docker, Singularity or Podman. + * Please only use Conda as a last resort i.e. when it's not possible to run the pipeline with Docker, Singularity, Podman, Shifter or Charliecloud. * A generic configuration profile to be used with [Conda](https://conda.io/docs/) * Pulls most software from [Bioconda](https://bioconda.github.io/) * `test` @@ -103,6 +109,8 @@ process { } ``` +To find the exact name of a process you wish to modify the compute resources, check the live-status of a nextflow run displayed on your terminal or check the nextflow error for a line like so: `Error executing process > 'bwa'`. In this case the name to specify in the custom config file is `bwa`. + See the main [Nextflow documentation](https://www.nextflow.io/docs/latest/config.html) for more information. If you are likely to be running `nf-core` pipelines regularly it may be a good idea to request that your custom config file is uploaded to the `nf-core/configs` git repository. Before you do this please can you test that the config file works with your pipeline of choice using the `-c` parameter (see definition above). You can then create a pull request to the `nf-core/configs` repository with the addition of your config file, associated documentation file (see examples in [`nf-core/configs/docs`](https://github.com/nf-core/configs/tree/master/docs)), and amending [`nfcore_custom.config`](https://github.com/nf-core/configs/blob/master/nfcore_custom.config) to include your custom profile. diff --git a/lib/Headers.groovy b/lib/Headers.groovy new file mode 100644 index 00000000..15d1d388 --- /dev/null +++ b/lib/Headers.groovy @@ -0,0 +1,43 @@ +/* + * This file holds several functions used to render the nf-core ANSI header. + */ + +class Headers { + + private static Map log_colours(Boolean monochrome_logs) { + Map colorcodes = [:] + colorcodes['reset'] = monochrome_logs ? '' : "\033[0m" + colorcodes['dim'] = monochrome_logs ? '' : "\033[2m" + colorcodes['black'] = monochrome_logs ? '' : "\033[0;30m" + colorcodes['green'] = monochrome_logs ? '' : "\033[0;32m" + colorcodes['yellow'] = monochrome_logs ? '' : "\033[0;33m" + colorcodes['yellow_bold'] = monochrome_logs ? '' : "\033[1;93m" + colorcodes['blue'] = monochrome_logs ? '' : "\033[0;34m" + colorcodes['purple'] = monochrome_logs ? '' : "\033[0;35m" + colorcodes['cyan'] = monochrome_logs ? '' : "\033[0;36m" + colorcodes['white'] = monochrome_logs ? '' : "\033[0;37m" + colorcodes['red'] = monochrome_logs ? '' : "\033[1;91m" + return colorcodes + } + + static String dashed_line(monochrome_logs) { + Map colors = log_colours(monochrome_logs) + return "-${colors.dim}----------------------------------------------------${colors.reset}-" + } + + static String nf_core(workflow, monochrome_logs) { + Map colors = log_colours(monochrome_logs) + String.format( + """\n + ${dashed_line(monochrome_logs)} + ${colors.green},--.${colors.black}/${colors.green},-.${colors.reset} + ${colors.blue} ___ __ __ __ ___ ${colors.green}/,-._.--~\'${colors.reset} + ${colors.blue} |\\ | |__ __ / ` / \\ |__) |__ ${colors.yellow}} {${colors.reset} + ${colors.blue} | \\| | \\__, \\__/ | \\ |___ ${colors.green}\\`-._,-`-,${colors.reset} + ${colors.green}`._,._,\'${colors.reset} + ${colors.purple} ${workflow.manifest.name} v${workflow.manifest.version}${colors.reset} + ${dashed_line(monochrome_logs)} + """.stripIndent() + ) + } +} diff --git a/lib/NfcoreSchema.groovy b/lib/NfcoreSchema.groovy new file mode 100644 index 00000000..78e8c65d --- /dev/null +++ b/lib/NfcoreSchema.groovy @@ -0,0 +1,571 @@ +/* + * This file holds several functions used to perform JSON parameter validation, help and summary rendering for the nf-core pipeline template. + */ + +import org.everit.json.schema.Schema +import org.everit.json.schema.loader.SchemaLoader +import org.everit.json.schema.ValidationException +import org.json.JSONObject +import org.json.JSONTokener +import org.json.JSONArray +import groovy.json.JsonSlurper +import groovy.json.JsonBuilder + +class NfcoreSchema { + + /* + * Function to loop over all parameters defined in schema and check + * whether the given paremeters adhere to the specificiations + */ + /* groovylint-disable-next-line UnusedPrivateMethodParameter */ + private static void validateParameters(params, jsonSchema, log) { + def has_error = false + //=====================================================================// + // Check for nextflow core params and unexpected params + def json = new File(jsonSchema).text + def Map schemaParams = (Map) new JsonSlurper().parseText(json).get('definitions') + def nf_params = [ + // Options for base `nextflow` command + 'bg', + 'c', + 'C', + 'config', + 'd', + 'D', + 'dockerize', + 'h', + 'log', + 'q', + 'quiet', + 'syslog', + 'v', + 'version', + + // Options for `nextflow run` command + 'ansi', + 'ansi-log', + 'bg', + 'bucket-dir', + 'c', + 'cache', + 'config', + 'dsl2', + 'dump-channels', + 'dump-hashes', + 'E', + 'entry', + 'latest', + 'lib', + 'main-script', + 'N', + 'name', + 'offline', + 'params-file', + 'pi', + 'plugins', + 'poll-interval', + 'pool-size', + 'profile', + 'ps', + 'qs', + 'queue-size', + 'r', + 'resume', + 'revision', + 'stdin', + 'stub', + 'stub-run', + 'test', + 'w', + 'with-charliecloud', + 'with-conda', + 'with-dag', + 'with-docker', + 'with-mpi', + 'with-notification', + 'with-podman', + 'with-report', + 'with-singularity', + 'with-timeline', + 'with-tower', + 'with-trace', + 'with-weblog', + 'without-docker', + 'without-podman', + 'work-dir' + ] + def unexpectedParams = [] + + // Collect expected parameters from the schema + def expectedParams = [] + for (group in schemaParams) { + for (p in group.value['properties']) { + expectedParams.push(p.key) + } + } + + for (specifiedParam in params.keySet()) { + // nextflow params + if (nf_params.contains(specifiedParam)) { + log.error "ERROR: You used a core Nextflow option with two hyphens: '--${specifiedParam}'. Please resubmit with '-${specifiedParam}'" + has_error = true + } + // unexpected params + def params_ignore = params.schema_ignore_params.split(',') + 'schema_ignore_params' + if (!expectedParams.contains(specifiedParam) && !params_ignore.contains(specifiedParam)) { + unexpectedParams.push(specifiedParam) + } + } + + //=====================================================================// + // Validate parameters against the schema + InputStream inputStream = new File(jsonSchema).newInputStream() + JSONObject rawSchema = new JSONObject(new JSONTokener(inputStream)) + + // Remove anything that's in params.schema_ignore_params + rawSchema = removeIgnoredParams(rawSchema, params) + + Schema schema = SchemaLoader.load(rawSchema) + + // Clean the parameters + def cleanedParams = cleanParameters(params) + + // Convert to JSONObject + def jsonParams = new JsonBuilder(cleanedParams) + JSONObject paramsJSON = new JSONObject(jsonParams.toString()) + + // Validate + try { + schema.validate(paramsJSON) + } catch (ValidationException e) { + println '' + log.error 'ERROR: Validation of pipeline parameters failed!' + JSONObject exceptionJSON = e.toJSON() + printExceptions(exceptionJSON, paramsJSON, log) + println '' + has_error = true + } + + // Check for unexpected parameters + if (unexpectedParams.size() > 0) { + Map colors = log_colours(params.monochrome_logs) + println '' + def warn_msg = 'Found unexpected parameters:' + for (unexpectedParam in unexpectedParams) { + warn_msg = warn_msg + "\n* --${unexpectedParam}: ${params[unexpectedParam].toString()}" + } + log.warn warn_msg + log.info "- ${colors.dim}Ignore this warning: params.schema_ignore_params = \"${unexpectedParams.join(',')}\" ${colors.reset}" + println '' + } + + if (has_error) { + System.exit(1) + } + } + + // Loop over nested exceptions and print the causingException + private static void printExceptions(exJSON, paramsJSON, log) { + def causingExceptions = exJSON['causingExceptions'] + if (causingExceptions.length() == 0) { + def m = exJSON['message'] =~ /required key \[([^\]]+)\] not found/ + // Missing required param + if (m.matches()) { + log.error "* Missing required parameter: --${m[0][1]}" + } + // Other base-level error + else if (exJSON['pointerToViolation'] == '#') { + log.error "* ${exJSON['message']}" + } + // Error with specific param + else { + def param = exJSON['pointerToViolation'] - ~/^#\// + def param_val = paramsJSON[param].toString() + log.error "* --${param}: ${exJSON['message']} (${param_val})" + } + } + for (ex in causingExceptions) { + printExceptions(ex, paramsJSON, log) + } + } + + // Remove an element from a JSONArray + private static JSONArray removeElement(jsonArray, element){ + def list = [] + int len = jsonArray.length() + for (int i=0;i + if(rawSchema.keySet().contains('definitions')){ + rawSchema.definitions.each { definition -> + for (key in definition.keySet()){ + if (definition[key].get("properties").keySet().contains(ignore_param)){ + // Remove the param to ignore + definition[key].get("properties").remove(ignore_param) + // If the param was required, change this + if (definition[key].has("required")) { + def cleaned_required = removeElement(definition[key].required, ignore_param) + definition[key].put("required", cleaned_required) + } + } + } + } + } + if(rawSchema.keySet().contains('properties') && rawSchema.get('properties').containsKey(ignore_param)) { + rawSchema.get("properties").remove(ignore_param) + } + if(rawSchema.keySet().contains('required') && rawSchema.required.contains(ignore_param)) { + def cleaned_required = removeElement(rawSchema.required, ignore_param) + rawSchema.put("required", cleaned_required) + } + } + return rawSchema + } + + private static Map cleanParameters(params) { + def new_params = params.getClass().newInstance(params) + for (p in params) { + // remove anything evaluating to false + if (!p['value']) { + new_params.remove(p.key) + } + // Cast MemoryUnit to String + if (p['value'].getClass() == nextflow.util.MemoryUnit) { + new_params.replace(p.key, p['value'].toString()) + } + // Cast Duration to String + if (p['value'].getClass() == nextflow.util.Duration) { + new_params.replace(p.key, p['value'].toString()) + } + // Cast LinkedHashMap to String + if (p['value'].getClass() == LinkedHashMap) { + new_params.replace(p.key, p['value'].toString()) + } + } + return new_params + } + + /* + * This method tries to read a JSON params file + */ + private static LinkedHashMap params_load(String json_schema) { + def params_map = new LinkedHashMap() + try { + params_map = params_read(json_schema) + } catch (Exception e) { + println "Could not read parameters settings from JSON. $e" + params_map = new LinkedHashMap() + } + return params_map + } + + private static Map log_colours(Boolean monochrome_logs) { + Map colorcodes = [:] + + // Reset / Meta + colorcodes['reset'] = monochrome_logs ? '' : "\033[0m" + colorcodes['bold'] = monochrome_logs ? '' : "\033[1m" + colorcodes['dim'] = monochrome_logs ? '' : "\033[2m" + colorcodes['underlined'] = monochrome_logs ? '' : "\033[4m" + colorcodes['blink'] = monochrome_logs ? '' : "\033[5m" + colorcodes['reverse'] = monochrome_logs ? '' : "\033[7m" + colorcodes['hidden'] = monochrome_logs ? '' : "\033[8m" + + // Regular Colors + colorcodes['black'] = monochrome_logs ? '' : "\033[0;30m" + colorcodes['red'] = monochrome_logs ? '' : "\033[0;31m" + colorcodes['green'] = monochrome_logs ? '' : "\033[0;32m" + colorcodes['yellow'] = monochrome_logs ? '' : "\033[0;33m" + colorcodes['blue'] = monochrome_logs ? '' : "\033[0;34m" + colorcodes['purple'] = monochrome_logs ? '' : "\033[0;35m" + colorcodes['cyan'] = monochrome_logs ? '' : "\033[0;36m" + colorcodes['white'] = monochrome_logs ? '' : "\033[0;37m" + + // Bold + colorcodes['bblack'] = monochrome_logs ? '' : "\033[1;30m" + colorcodes['bred'] = monochrome_logs ? '' : "\033[1;31m" + colorcodes['bgreen'] = monochrome_logs ? '' : "\033[1;32m" + colorcodes['byellow'] = monochrome_logs ? '' : "\033[1;33m" + colorcodes['bblue'] = monochrome_logs ? '' : "\033[1;34m" + colorcodes['bpurple'] = monochrome_logs ? '' : "\033[1;35m" + colorcodes['bcyan'] = monochrome_logs ? '' : "\033[1;36m" + colorcodes['bwhite'] = monochrome_logs ? '' : "\033[1;37m" + + // Underline + colorcodes['ublack'] = monochrome_logs ? '' : "\033[4;30m" + colorcodes['ured'] = monochrome_logs ? '' : "\033[4;31m" + colorcodes['ugreen'] = monochrome_logs ? '' : "\033[4;32m" + colorcodes['uyellow'] = monochrome_logs ? '' : "\033[4;33m" + colorcodes['ublue'] = monochrome_logs ? '' : "\033[4;34m" + colorcodes['upurple'] = monochrome_logs ? '' : "\033[4;35m" + colorcodes['ucyan'] = monochrome_logs ? '' : "\033[4;36m" + colorcodes['uwhite'] = monochrome_logs ? '' : "\033[4;37m" + + // High Intensity + colorcodes['iblack'] = monochrome_logs ? '' : "\033[0;90m" + colorcodes['ired'] = monochrome_logs ? '' : "\033[0;91m" + colorcodes['igreen'] = monochrome_logs ? '' : "\033[0;92m" + colorcodes['iyellow'] = monochrome_logs ? '' : "\033[0;93m" + colorcodes['iblue'] = monochrome_logs ? '' : "\033[0;94m" + colorcodes['ipurple'] = monochrome_logs ? '' : "\033[0;95m" + colorcodes['icyan'] = monochrome_logs ? '' : "\033[0;96m" + colorcodes['iwhite'] = monochrome_logs ? '' : "\033[0;97m" + + // Bold High Intensity + colorcodes['biblack'] = monochrome_logs ? '' : "\033[1;90m" + colorcodes['bired'] = monochrome_logs ? '' : "\033[1;91m" + colorcodes['bigreen'] = monochrome_logs ? '' : "\033[1;92m" + colorcodes['biyellow'] = monochrome_logs ? '' : "\033[1;93m" + colorcodes['biblue'] = monochrome_logs ? '' : "\033[1;94m" + colorcodes['bipurple'] = monochrome_logs ? '' : "\033[1;95m" + colorcodes['bicyan'] = monochrome_logs ? '' : "\033[1;96m" + colorcodes['biwhite'] = monochrome_logs ? '' : "\033[1;97m" + + return colorcodes + } + + static String dashed_line(monochrome_logs) { + Map colors = log_colours(monochrome_logs) + return "-${colors.dim}----------------------------------------------------${colors.reset}-" + } + + /* + Method to actually read in JSON file using Groovy. + Group (as Key), values are all parameters + - Parameter1 as Key, Description as Value + - Parameter2 as Key, Description as Value + .... + Group + - + */ + private static LinkedHashMap params_read(String json_schema) throws Exception { + def json = new File(json_schema).text + def Map schema_definitions = (Map) new JsonSlurper().parseText(json).get('definitions') + def Map schema_properties = (Map) new JsonSlurper().parseText(json).get('properties') + /* Tree looks like this in nf-core schema + * definitions <- this is what the first get('definitions') gets us + group 1 + title + description + properties + parameter 1 + type + description + parameter 2 + type + description + group 2 + title + description + properties + parameter 1 + type + description + * properties <- parameters can also be ungrouped, outside of definitions + parameter 1 + type + description + */ + + // Grouped params + def params_map = new LinkedHashMap() + schema_definitions.each { key, val -> + def Map group = schema_definitions."$key".properties // Gets the property object of the group + def title = schema_definitions."$key".title + def sub_params = new LinkedHashMap() + group.each { innerkey, value -> + sub_params.put(innerkey, value) + } + params_map.put(title, sub_params) + } + + // Ungrouped params + def ungrouped_params = new LinkedHashMap() + schema_properties.each { innerkey, value -> + ungrouped_params.put(innerkey, value) + } + params_map.put("Other parameters", ungrouped_params) + + return params_map + } + + /* + * Get maximum number of characters across all parameter names + */ + private static Integer params_max_chars(params_map) { + Integer max_chars = 0 + for (group in params_map.keySet()) { + def group_params = params_map.get(group) // This gets the parameters of that particular group + for (param in group_params.keySet()) { + if (param.size() > max_chars) { + max_chars = param.size() + } + } + } + return max_chars + } + + /* + * Beautify parameters for --help + */ + private static String params_help(workflow, params, json_schema, command) { + Map colors = log_colours(params.monochrome_logs) + Integer num_hidden = 0 + String output = '' + output += 'Typical pipeline command:\n\n' + output += " ${colors.cyan}${command}${colors.reset}\n\n" + Map params_map = params_load(json_schema) + Integer max_chars = params_max_chars(params_map) + 1 + Integer desc_indent = max_chars + 14 + Integer dec_linewidth = 160 - desc_indent + for (group in params_map.keySet()) { + Integer num_params = 0 + String group_output = colors.underlined + colors.bold + group + colors.reset + '\n' + def group_params = params_map.get(group) // This gets the parameters of that particular group + for (param in group_params.keySet()) { + if (group_params.get(param).hidden && !params.show_hidden_params) { + num_hidden += 1 + continue; + } + def type = '[' + group_params.get(param).type + ']' + def description = group_params.get(param).description + def defaultValue = group_params.get(param).default ? " [default: " + group_params.get(param).default.toString() + "]" : '' + def description_default = description + colors.dim + defaultValue + colors.reset + // Wrap long description texts + // Loosely based on https://dzone.com/articles/groovy-plain-text-word-wrap + if (description_default.length() > dec_linewidth){ + List olines = [] + String oline = "" // " " * indent + description_default.split(" ").each() { wrd -> + if ((oline.size() + wrd.size()) <= dec_linewidth) { + oline += wrd + " " + } else { + olines += oline + oline = wrd + " " + } + } + olines += oline + description_default = olines.join("\n" + " " * desc_indent) + } + group_output += " --" + param.padRight(max_chars) + colors.dim + type.padRight(10) + colors.reset + description_default + '\n' + num_params += 1 + } + group_output += '\n' + if (num_params > 0){ + output += group_output + } + } + output += dashed_line(params.monochrome_logs) + if (num_hidden > 0){ + output += colors.dim + "\n Hiding $num_hidden params, use --show_hidden_params to show.\n" + colors.reset + output += dashed_line(params.monochrome_logs) + } + return output + } + + /* + * Groovy Map summarising parameters/workflow options used by the pipeline + */ + private static LinkedHashMap params_summary_map(workflow, params, json_schema) { + // Get a selection of core Nextflow workflow options + def Map workflow_summary = [:] + if (workflow.revision) { + workflow_summary['revision'] = workflow.revision + } + workflow_summary['runName'] = workflow.runName + if (workflow.containerEngine) { + workflow_summary['containerEngine'] = "$workflow.containerEngine" + } + if (workflow.container) { + workflow_summary['container'] = "$workflow.container" + } + workflow_summary['launchDir'] = workflow.launchDir + workflow_summary['workDir'] = workflow.workDir + workflow_summary['projectDir'] = workflow.projectDir + workflow_summary['userName'] = workflow.userName + workflow_summary['profile'] = workflow.profile + workflow_summary['configFiles'] = workflow.configFiles.join(', ') + + // Get pipeline parameters defined in JSON Schema + def Map params_summary = [:] + def blacklist = ['hostnames'] + def params_map = params_load(json_schema) + for (group in params_map.keySet()) { + def sub_params = new LinkedHashMap() + def group_params = params_map.get(group) // This gets the parameters of that particular group + for (param in group_params.keySet()) { + if (params.containsKey(param) && !blacklist.contains(param)) { + def params_value = params.get(param) + def schema_value = group_params.get(param).default + def param_type = group_params.get(param).type + if (schema_value == null) { + if (param_type == 'boolean') { + schema_value = false + } + if (param_type == 'string') { + schema_value = '' + } + if (param_type == 'integer') { + schema_value = 0 + } + } else { + if (param_type == 'string') { + if (schema_value.contains('$projectDir') || schema_value.contains('${projectDir}')) { + def sub_string = schema_value.replace('\$projectDir', '') + sub_string = sub_string.replace('\${projectDir}', '') + if (params_value.contains(sub_string)) { + schema_value = params_value + } + } + if (schema_value.contains('$params.outdir') || schema_value.contains('${params.outdir}')) { + def sub_string = schema_value.replace('\$params.outdir', '') + sub_string = sub_string.replace('\${params.outdir}', '') + if ("${params.outdir}${sub_string}" == params_value) { + schema_value = params_value + } + } + } + } + + if (params_value != schema_value) { + sub_params.put("$param", params_value) + } + } + } + params_summary.put(group, sub_params) + } + return [ 'Core Nextflow options' : workflow_summary ] << params_summary + } + + /* + * Beautify parameters for summary and return as string + */ + private static String params_summary_log(workflow, params, json_schema) { + String output = '' + def params_map = params_summary_map(workflow, params, json_schema) + def max_chars = params_max_chars(params_map) + for (group in params_map.keySet()) { + def group_params = params_map.get(group) // This gets the parameters of that particular group + if (group_params) { + output += group + '\n' + for (param in group_params.keySet()) { + output += " \u001B[1m" + param.padRight(max_chars) + ": \u001B[1m" + group_params.get(param) + '\n' + } + output += '\n' + } + } + output += "[Only displaying parameters that differ from pipeline default]\n" + output += dashed_line(params.monochrome_logs) + output += '\n\n' + dashed_line(params.monochrome_logs) + return output + } + +} diff --git a/lib/nfcore_external_java_deps.jar b/lib/nfcore_external_java_deps.jar new file mode 100644 index 0000000000000000000000000000000000000000..805c8bb5e4fd43a12a5891eea5a68788309629b0 GIT binary patch literal 2291171 zcma%i1CS<7mu}m(-Ea4_?dhK8v~AnAZQHhO+qP}n-E-&Lf8*Yb{bM(7MpZ;s){{|L znGpxiIY(9k6buar3JMCSNkdW|=)W{*ATS^)VMTsgF=-L{?{Od?P#{?eNT`1hApZ%I z{U0Zz{YCuW$x{5%Vj{u{igZ#U4^op8k`lCZbMO+hRMV3)4e|_&%)3YSbmCGpVp4NX 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command for running the pipeline is as follows: - - nextflow run nf-core/bacass --input '*_R{1,2}.fastq.gz' -profile docker - - Mandatory arguments: - --input [file] Path to input data (must be surrounded with quotes) - -profile [str] Configuration profile to use. Can use multiple (comma separated) - Available: conda, docker, singularity, test, awsbatch, and more - - Options: - --genome [str] Name of iGenomes reference - --single_end [bool] Specifies that the input is single-end reads - - References If not specified in the configuration file or you wish to overwrite any of the references - --fasta [file] Path to fasta reference - - Other options: - --outdir [file] The output directory where the results will be saved - --publish_dir_mode [str] Mode for publishing results in the output directory. Available: symlink, rellink, link, copy, copyNoFollow, move (Default: copy) - --email [email] Set this parameter to your e-mail address to get a summary e-mail with details of the run sent to you when the workflow exits - --email_on_fail [email] Same as --email, except only send mail if the workflow is not successful - --max_multiqc_email_size [str] Threshold size for MultiQC report to be attached in notification email. If file generated by pipeline exceeds the threshold, it will not be attached (Default: 25MB) - -name [str] Name for the pipeline run. If not specified, Nextflow will automatically generate a random mnemonic - - AWSBatch options: - --awsqueue [str] The AWSBatch JobQueue that needs to be set when running on AWSBatch - --awsregion [str] The AWS Region for your AWS Batch job to run on - --awscli [str] Path to the AWS CLI tool - """.stripIndent() -} +log.info Headers.nf_core(workflow, params.monochrome_logs) -// Show help message +//////////////////////////////////////////////////// +/* -- PRINT HELP -- */ +////////////////////////////////////////////////////+ +def json_schema = "$projectDir/nextflow_schema.json" if (params.help) { - helpMessage() + def command = "nextflow run nf-core/bacass --input '*_R{1,2}.fastq.gz' -profile docker" + log.info NfcoreSchema.params_help(workflow, params, json_schema, command) exit 0 } -/* - * SET UP CONFIGURATION VARIABLES - */ +//////////////////////////////////////////////////// +/* -- VALIDATE PARAMETERS -- */ +////////////////////////////////////////////////////+ +if (params.validate_params) { + NfcoreSchema.validateParameters(params, json_schema, log) +} + +//////////////////////////////////////////////////// +/* -- Collect configuration parameters -- */ +//////////////////////////////////////////////////// // Check if genome exists in the config file if (params.genomes && params.genome && !params.genomes.containsKey(params.genome)) { - exit 1, "The provided genome '${params.genome}' is not available in the iGenomes file. Currently the available genomes are ${params.genomes.keySet().join(", ")}" + exit 1, "The provided genome '${params.genome}' is not available in the iGenomes file. Currently the available genomes are ${params.genomes.keySet().join(', ')}" } // TODO nf-core: Add any reference files that are needed @@ -73,22 +48,15 @@ if (params.genomes && params.genome && !params.genomes.containsKey(params.genome params.fasta = params.genome ? params.genomes[ params.genome ].fasta ?: false : false if (params.fasta) { ch_fasta = file(params.fasta, checkIfExists: true) } -// Has the run name been specified by the user? -// this has the bonus effect of catching both -name and --name -custom_runName = params.name -if (!(workflow.runName ==~ /[a-z]+_[a-z]+/)) { - custom_runName = workflow.runName -} - // Check AWS batch settings if (workflow.profile.contains('awsbatch')) { // AWSBatch sanity checking - if (!params.awsqueue || !params.awsregion) exit 1, "Specify correct --awsqueue and --awsregion parameters on AWSBatch!" + if (!params.awsqueue || !params.awsregion) exit 1, 'Specify correct --awsqueue and --awsregion parameters on AWSBatch!' // Check outdir paths to be S3 buckets if running on AWSBatch // related: https://github.com/nextflow-io/nextflow/issues/813 - if (!params.outdir.startsWith('s3:')) exit 1, "Outdir not on S3 - specify S3 Bucket to run on AWSBatch!" + if (!params.outdir.startsWith('s3:')) exit 1, 'Outdir not on S3 - specify S3 Bucket to run on AWSBatch!' // Prevent trace files to be stored on S3 since S3 does not support rolling files. - if (params.tracedir.startsWith('s3:')) exit 1, "Specify a local tracedir or run without trace! S3 cannot be used for tracefiles." + if (params.tracedir.startsWith('s3:')) exit 1, 'Specify a local tracedir or run without trace! S3 cannot be used for tracefiles.' } // Stage config files @@ -105,13 +73,13 @@ if (params.input_paths) { Channel .from(params.input_paths) .map { row -> [ row[0], [ file(row[1][0], checkIfExists: true) ] ] } - .ifEmpty { exit 1, "params.input_paths was empty - no input files supplied" } + .ifEmpty { exit 1, 'params.input_paths was empty - no input files supplied' } .into { ch_read_files_fastqc; ch_read_files_trimming } } else { Channel .from(params.input_paths) .map { row -> [ row[0], [ file(row[1][0], checkIfExists: true), file(row[1][1], checkIfExists: true) ] ] } - .ifEmpty { exit 1, "params.input_paths was empty - no input files supplied" } + .ifEmpty { exit 1, 'params.input_paths was empty - no input files supplied' } .into { ch_read_files_fastqc; ch_read_files_trimming } } } else { @@ -121,11 +89,15 @@ if (params.input_paths) { .into { ch_read_files_fastqc; ch_read_files_trimming } } +//////////////////////////////////////////////////// +/* -- PRINT PARAMETER SUMMARY -- */ +//////////////////////////////////////////////////// +log.info NfcoreSchema.params_summary_log(workflow, params, json_schema) + // Header log info -log.info nfcoreHeader() def summary = [:] if (workflow.revision) summary['Pipeline Release'] = workflow.revision -summary['Run Name'] = custom_runName ?: workflow.runName +summary['Run Name'] = workflow.runName // TODO nf-core: Report custom parameters here summary['Input'] = params.input summary['Fasta Ref'] = params.fasta @@ -152,8 +124,6 @@ if (params.email || params.email_on_fail) { summary['E-mail on failure'] = params.email_on_fail summary['MultiQC maxsize'] = params.max_multiqc_email_size } -log.info summary.collect { k,v -> "${k.padRight(18)}: $v" }.join("\n") -log.info "-\033[2m--------------------------------------------------\033[0m-" // Check the hostnames against configured profiles checkHostname() @@ -180,13 +150,13 @@ Channel.from(summary.collect{ [it.key, it.value] }) process get_software_versions { publishDir "${params.outdir}/pipeline_info", mode: params.publish_dir_mode, saveAs: { filename -> - if (filename.indexOf(".csv") > 0) filename + if (filename.indexOf('.csv') > 0) filename else null - } + } output: file 'software_versions_mqc.yaml' into ch_software_versions_yaml - file "software_versions.csv" + file 'software_versions.csv' script: // TODO nf-core: Get all tools to print their version number here @@ -207,14 +177,14 @@ process fastqc { label 'process_medium' publishDir "${params.outdir}/fastqc", mode: params.publish_dir_mode, saveAs: { filename -> - filename.indexOf(".zip") > 0 ? "zips/$filename" : "$filename" - } + filename.indexOf('.zip') > 0 ? "zips/$filename" : "$filename" + } input: set val(name), file(reads) from ch_read_files_fastqc output: - file "*_fastqc.{zip,html}" into ch_fastqc_results + file '*_fastqc.{zip,html}' into ch_fastqc_results script: """ @@ -242,8 +212,12 @@ process multiqc { file "multiqc_plots" script: - rtitle = custom_runName ? "--title \"$custom_runName\"" : '' - rfilename = custom_runName ? "--filename " + custom_runName.replaceAll('\\W','_').replaceAll('_+','_') + "_multiqc_report" : '' + rtitle = '' + rfilename = '' + if (!(workflow.runName ==~ /[a-z]+_[a-z]+/)) { + rtitle = "--title \"${workflow.runName}\"" + rfilename = "--filename " + workflow.runName.replaceAll('\\W','_').replaceAll('_+','_') + "_multiqc_report" + } custom_config_file = params.multiqc_config ? "--config $mqc_custom_config" : '' // TODO nf-core: Specify which MultiQC modules to use with -m for a faster run time """ @@ -262,7 +236,7 @@ process output_documentation { file images from ch_output_docs_images output: - file "results_description.html" + file 'results_description.html' script: """ @@ -282,7 +256,7 @@ workflow.onComplete { } def email_fields = [:] email_fields['version'] = workflow.manifest.version - email_fields['runName'] = custom_runName ?: workflow.runName + email_fields['runName'] = workflow.runName email_fields['success'] = workflow.success email_fields['dateComplete'] = workflow.complete email_fields['duration'] = workflow.duration @@ -389,28 +363,9 @@ workflow.onComplete { } - -def nfcoreHeader() { - // Log colors ANSI codes - c_black = params.monochrome_logs ? '' : "\033[0;30m"; - c_blue = params.monochrome_logs ? '' : "\033[0;34m"; - c_cyan = params.monochrome_logs ? '' : "\033[0;36m"; - c_dim = params.monochrome_logs ? '' : "\033[2m"; - c_green = params.monochrome_logs ? '' : "\033[0;32m"; - c_purple = params.monochrome_logs ? '' : "\033[0;35m"; - c_reset = params.monochrome_logs ? '' : "\033[0m"; - c_white = params.monochrome_logs ? '' : "\033[0;37m"; - c_yellow = params.monochrome_logs ? '' : "\033[0;33m"; - - return """ -${c_dim}--------------------------------------------------${c_reset}- - ${c_green},--.${c_black}/${c_green},-.${c_reset} - ${c_blue} ___ __ __ __ ___ ${c_green}/,-._.--~\'${c_reset} - ${c_blue} |\\ | |__ __ / ` / \\ |__) |__ ${c_yellow}} {${c_reset} - ${c_blue} | \\| | \\__, \\__/ | \\ |___ ${c_green}\\`-._,-`-,${c_reset} - ${c_green}`._,._,\'${c_reset} - ${c_purple} nf-core/bacass v${workflow.manifest.version}${c_reset} - -${c_dim}--------------------------------------------------${c_reset}- - """.stripIndent() +workflow.onError { + // Print unexpected parameters - easiest is to just rerun validation + NfcoreSchema.validateParameters(params, json_schema, log) } def checkHostname() { @@ -419,15 +374,15 @@ def checkHostname() { def c_red = params.monochrome_logs ? '' : "\033[1;91m" def c_yellow_bold = params.monochrome_logs ? '' : "\033[1;93m" if (params.hostnames) { - def hostname = "hostname".execute().text.trim() + def hostname = 'hostname'.execute().text.trim() params.hostnames.each { prof, hnames -> hnames.each { hname -> if (hostname.contains(hname) && !workflow.profile.contains(prof)) { - log.error "====================================================\n" + + log.error '====================================================\n' + " ${c_red}WARNING!${c_reset} You are running with `-profile $workflow.profile`\n" + " but your machine hostname is ${c_white}'$hostname'${c_reset}\n" + " ${c_yellow_bold}It's highly recommended that you use `-profile $prof${c_reset}`\n" + - "============================================================" + '============================================================' } } } diff --git a/nextflow.config b/nextflow.config index 0a0a6c48..a3cb4f30 100644 --- a/nextflow.config +++ b/nextflow.config @@ -11,13 +11,13 @@ params { // Workflow flags // TODO nf-core: Specify your pipeline's command line flags genome = false - input = "data/*{1,2}.fastq.gz" + input = null + input_paths = null single_end = false outdir = './results' publish_dir_mode = 'copy' // Boilerplate options - name = false multiqc_config = false email = false email_on_fail = false @@ -34,6 +34,9 @@ params { config_profile_description = false config_profile_contact = false config_profile_url = false + validate_params = true + show_hidden_params = false + schema_ignore_params = 'genomes,input_paths' // Defaults only, expecting to be overwritten max_memory = 128.GB @@ -57,10 +60,21 @@ try { } profiles { - conda { process.conda = "$projectDir/environment.yml" } + conda { + docker.enabled = false + singularity.enabled = false + podman.enabled = false + shifter.enabled = false + charliecloud = false + process.conda = "$projectDir/environment.yml" + } debug { process.beforeScript = 'echo $HOSTNAME' } docker { docker.enabled = true + singularity.enabled = false + podman.enabled = false + shifter.enabled = false + charliecloud.enabled = false // Avoid this error: // WARNING: Your kernel does not support swap limit capabilities or the cgroup is not mounted. Memory limited without swap. // Testing this in nf-core after discussion here https://github.com/nf-core/tools/pull/351 @@ -68,11 +82,33 @@ profiles { docker.runOptions = '-u \$(id -u):\$(id -g)' } singularity { + docker.enabled = false singularity.enabled = true + podman.enabled = false + shifter.enabled = false + charliecloud.enabled = false singularity.autoMounts = true } podman { + singularity.enabled = false + docker.enabled = false podman.enabled = true + shifter.enabled = false + charliecloud = false + } + shifter { + singularity.enabled = false + docker.enabled = false + podman.enabled = false + shifter.enabled = true + charliecloud.enabled = false + } + charliecloud { + singularity.enabled = false + docker.enabled = false + podman.enabled = false + shifter.enabled = false + charliecloud.enabled = true } test { includeConfig 'conf/test.config' } test_full { includeConfig 'conf/test_full.config' } diff --git a/nextflow_schema.json b/nextflow_schema.json index b1191d1f..d043e321 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -104,12 +104,12 @@ "move" ] }, - "name": { - "type": "string", - "description": "Workflow name.", - "fa_icon": "fas fa-fingerprint", - "hidden": true, - "help_text": "A custom name for the pipeline run. Unlike the core nextflow `-name` option with one hyphen this parameter can be reused multiple times, for example if using `-resume`. Passed through to steps such as MultiQC and used for things like report filenames and titles." + "validate_params": { + "type": "boolean", + "description": "Boolean whether to validate parameters against the schema at runtime", + "default": true, + "fa_icon": "fas fa-check-square", + "hidden": true }, "email_on_fail": { "type": "string", @@ -153,6 +153,13 @@ "default": "${params.outdir}/pipeline_info", "fa_icon": "fas fa-cogs", "hidden": true + }, + "show_hidden_params": { + "type": "boolean", + "fa_icon": "far fa-eye-slash", + "description": "Show all params when using `--help`", + "hidden": true, + "help_text": "By default, parameters set as _hidden_ in the schema are not shown on the command line when a user runs with `--help`. Specifying this option will tell the pipeline to show all parameters." } } }, @@ -176,6 +183,7 @@ "description": "Maximum amount of memory that can be requested for any single job.", "default": "128.GB", "fa_icon": "fas fa-memory", + "pattern": "^[\\d\\.]+\\s*.(K|M|G|T)?B$", "hidden": true, "help_text": "Use to set an upper-limit for the memory requirement for each process. Should be a string in the format integer-unit e.g. `--max_memory '8.GB'`" }, @@ -184,6 +192,7 @@ "description": "Maximum amount of time that can be requested for any single job.", "default": "240.h", "fa_icon": "far fa-clock", + "pattern": "^[\\d\\.]+\\.*(s|m|h|d)$", "hidden": true, "help_text": "Use to set an upper-limit for the time requirement for each process. Should be a string in the format integer-unit e.g. `--max_time '2.h'`" } @@ -218,6 +227,12 @@ "hidden": true, "fa_icon": "fas fa-users-cog" }, + "config_profile_name": { + "type": "string", + "description": "Institutional config name.", + "hidden": true, + "fa_icon": "fas fa-users-cog" + }, "config_profile_description": { "type": "string", "description": "Institutional config description.", From be7bbe11cee060ea4d40e19ad974569bfe89e44b Mon Sep 17 00:00:00 2001 From: nf-core-bot Date: Fri, 19 Mar 2021 13:07:30 +0000 Subject: [PATCH 04/81] Template update for nf-core/tools version 1.13.1 --- .github/workflows/branch.yml | 12 ++++++++++-- Dockerfile | 2 +- 2 files changed, 11 insertions(+), 3 deletions(-) diff --git a/.github/workflows/branch.yml b/.github/workflows/branch.yml index 3f0e2153..453a14f4 100644 --- a/.github/workflows/branch.yml +++ b/.github/workflows/branch.yml @@ -23,11 +23,19 @@ jobs: uses: mshick/add-pr-comment@v1 with: message: | + ## This PR is against the `master` branch :x: + + * Do not close this PR + * Click _Edit_ and change the `base` to `dev` + * This CI test will remain failed until you push a new commit + + --- + Hi @${{ github.event.pull_request.user.login }}, - It looks like this pull-request is has been made against the ${{github.event.pull_request.base.repo.full_name }} `master` branch. + It looks like this pull-request is has been made against the [${{github.event.pull_request.head.repo.full_name }}](https://github.com/${{github.event.pull_request.head.repo.full_name }}) `master` branch. The `master` branch on nf-core repositories should always contain code from the latest release. - Because of this, PRs to `master` are only allowed if they come from the ${{github.event.pull_request.base.repo.full_name }} `dev` branch. + Because of this, PRs to `master` are only allowed if they come from the [${{github.event.pull_request.head.repo.full_name }}](https://github.com/${{github.event.pull_request.head.repo.full_name }}) `dev` branch. You do not need to close this PR, you can change the target branch to `dev` by clicking the _"Edit"_ button at the top of this page. Note that even after this, the test will continue to show as failing until you push a new commit. diff --git a/Dockerfile b/Dockerfile index ea598a79..c244648f 100644 --- a/Dockerfile +++ b/Dockerfile @@ -1,4 +1,4 @@ -FROM nfcore/base:1.13 +FROM nfcore/base:1.13.1 LABEL authors="Andreas Wilm, Alexander Peltzer" \ description="Docker image containing all software requirements for the nf-core/bacass pipeline" From dc2b066af6a5b864f731122ff733b38fc867d12c Mon Sep 17 00:00:00 2001 From: Ramon Rivera Date: Mon, 22 Mar 2021 21:47:40 -0700 Subject: [PATCH 05/81] Update README.md --- README.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/README.md b/README.md index d3415746..7dc8873a 100644 --- a/README.md +++ b/README.md @@ -39,7 +39,7 @@ The pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool The nf-core/bacass pipeline comes with documentation about the pipeline, found in the `docs/` directory: -The nf-core/bacass pipeline comes with documentation about the pipeline which you can read at [https://nf-core/bacass/docs](https://nf-core/bacass/docs) or find in the [`docs/` directory](docs). +The nf-core/bacass pipeline comes with documentation about the pipeline which you can read at [https://nf-co.re/bacass/usage](https://nf-co.re/bacass/usage) or find in the [`docs/` directory](docs). ## Credits From ceebac0341841490314443746659bce56a6cca26 Mon Sep 17 00:00:00 2001 From: nf-core-bot Date: Tue, 23 Mar 2021 15:21:13 +0000 Subject: [PATCH 06/81] Template update for nf-core/tools version 1.13.2 --- .github/workflows/ci.yml | 2 +- .github/workflows/linting.yml | 57 ++++++++++++++++++++++++++++++++- Dockerfile | 2 +- bin/markdown_to_html.py | 0 bin/scrape_software_versions.py | 0 lib/NfcoreSchema.groovy | 2 +- 6 files changed, 59 insertions(+), 4 deletions(-) mode change 100644 => 100755 bin/markdown_to_html.py mode change 100644 => 100755 bin/scrape_software_versions.py diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index cc30f960..9abb167d 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -20,7 +20,7 @@ jobs: strategy: matrix: # Nextflow versions: check pipeline minimum and current latest - nxf_ver: ['20.04.0', ''] + nxf_ver: ['20.04.0', '21.03.0-edge'] steps: - name: Check out pipeline code uses: actions/checkout@v2 diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml index 58e281df..fcde400c 100644 --- a/.github/workflows/linting.yml +++ b/.github/workflows/linting.yml @@ -19,6 +19,34 @@ jobs: run: npm install -g markdownlint-cli - name: Run Markdownlint run: markdownlint ${GITHUB_WORKSPACE} -c ${GITHUB_WORKSPACE}/.github/markdownlint.yml + + # If the above check failed, post a comment on the PR explaining the failure + - name: Post PR comment + if: failure() + uses: mshick/add-pr-comment@v1 + with: + message: | + ## Markdown linting is failing + + To keep the code consistent with lots of contributors, we run automated code consistency checks. + To fix this CI test, please run: + + * Install `markdownlint-cli` + * On Mac: `brew install markdownlint-cli` + * Everything else: [Install `npm`](https://www.npmjs.com/get-npm) then [install `markdownlint-cli`](https://www.npmjs.com/package/markdownlint-cli) (`npm install -g markdownlint-cli`) + * Fix the markdown errors + * Automatically: `markdownlint . --config .github/markdownlint.yml --fix` + * Manually resolve anything left from `markdownlint . --config .github/markdownlint.yml` + + Once you push these changes the test should pass, and you can hide this comment :+1: + + We highly recommend setting up markdownlint in your code editor so that this formatting is done automatically on save. Ask about it on Slack for help! + + Thanks again for your contribution! + repo-token: ${{ secrets.GITHUB_TOKEN }} + allow-repeats: false + + YAML: runs-on: ubuntu-latest steps: @@ -29,7 +57,34 @@ jobs: - name: Install yaml-lint run: npm install -g yaml-lint - name: Run yaml-lint - run: yamllint $(find ${GITHUB_WORKSPACE} -type f -name "*.yml") + run: yamllint $(find ${GITHUB_WORKSPACE} -type f -name "*.yml" -o -name "*.yaml") + + # If the above check failed, post a comment on the PR explaining the failure + - name: Post PR comment + if: failure() + uses: mshick/add-pr-comment@v1 + with: + message: | + ## YAML linting is failing + + To keep the code consistent with lots of contributors, we run automated code consistency checks. + To fix this CI test, please run: + + * Install `yaml-lint` + * [Install `npm`](https://www.npmjs.com/get-npm) then [install `yaml-lint`](https://www.npmjs.com/package/yaml-lint) (`npm install -g yaml-lint`) + * Fix the markdown errors + * Run the test locally: `yamllint $(find . -type f -name "*.yml" -o -name "*.yaml")` + * Fix any reported errors in your YAML files + + Once you push these changes the test should pass, and you can hide this comment :+1: + + We highly recommend setting up yaml-lint in your code editor so that this formatting is done automatically on save. Ask about it on Slack for help! + + Thanks again for your contribution! + repo-token: ${{ secrets.GITHUB_TOKEN }} + allow-repeats: false + + nf-core: runs-on: ubuntu-latest steps: diff --git a/Dockerfile b/Dockerfile index c244648f..9b54f7f3 100644 --- a/Dockerfile +++ b/Dockerfile @@ -1,4 +1,4 @@ -FROM nfcore/base:1.13.1 +FROM nfcore/base:1.13.2 LABEL authors="Andreas Wilm, Alexander Peltzer" \ description="Docker image containing all software requirements for the nf-core/bacass pipeline" diff --git a/bin/markdown_to_html.py b/bin/markdown_to_html.py old mode 100644 new mode 100755 diff --git a/bin/scrape_software_versions.py b/bin/scrape_software_versions.py old mode 100644 new mode 100755 diff --git a/lib/NfcoreSchema.groovy b/lib/NfcoreSchema.groovy index 78e8c65d..54935ec8 100644 --- a/lib/NfcoreSchema.groovy +++ b/lib/NfcoreSchema.groovy @@ -219,7 +219,7 @@ class NfcoreSchema { } } } - if(rawSchema.keySet().contains('properties') && rawSchema.get('properties').containsKey(ignore_param)) { + if(rawSchema.keySet().contains('properties') && rawSchema.get('properties').keySet().contains(ignore_param)) { rawSchema.get("properties").remove(ignore_param) } if(rawSchema.keySet().contains('required') && rawSchema.required.contains(ignore_param)) { From 0f9ba31efca5d86e952a2cb601c830e117e37830 Mon Sep 17 00:00:00 2001 From: nf-core-bot Date: Thu, 25 Mar 2021 18:17:15 +0000 Subject: [PATCH 07/81] Template update for nf-core/tools version 1.13.3 --- Dockerfile | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/Dockerfile b/Dockerfile index 9b54f7f3..e8f9e174 100644 --- a/Dockerfile +++ b/Dockerfile @@ -1,4 +1,4 @@ -FROM nfcore/base:1.13.2 +FROM nfcore/base:1.13.3 LABEL authors="Andreas Wilm, Alexander Peltzer" \ description="Docker image containing all software requirements for the nf-core/bacass pipeline" From 87f2e2523c01a56da82e77509b7ba51f0e651086 Mon Sep 17 00:00:00 2001 From: MaxUlysse Date: Mon, 26 Apr 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z&J6DVwG3b@`q|5}i4#pC83Mw9CF-(R;JJ{~ffIi_fg=#9z#x2Hd4|El7npp22bdYW zSiY+rIKL|DBP}i{Sa`0Dn?c-a9`Hb3FtCsS`w7IBLEJ5dxVY>fa6SN{k{z)dc~qeT zBuoyB1`qH6H!v7YJxmUekQhxAqa_9CVpt$37%fPNtv>$AclDWEdXt;}78G!vu6{1- HoD!M - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - image/svg+xml - - - - - - - Simple bacterial assembly and annotation pipeline - bacass - - - - - - - - - - - - - - - - - - - - - - - - - From b4b1d3a70a93e0e873c343c9197a491733020087 Mon Sep 17 00:00:00 2001 From: nf-core-bot Date: Tue, 11 May 2021 11:09:51 +0000 Subject: [PATCH 09/81] Template update for nf-core/tools version 1.14 --- .github/PULL_REQUEST_TEMPLATE.md | 7 +++-- .github/workflows/ci.yml | 5 ++- Dockerfile | 2 +- README.md | 2 +- lib/NfcoreSchema.groovy | 54 +++++++++++++++++--------------- main.nf | 4 +-- nextflow.config | 16 +++++----- nextflow_schema.json | 6 ++-- 8 files changed, 52 insertions(+), 44 deletions(-) diff --git a/.github/PULL_REQUEST_TEMPLATE.md b/.github/PULL_REQUEST_TEMPLATE.md index e8ed6a41..bac96797 100644 --- a/.github/PULL_REQUEST_TEMPLATE.md +++ b/.github/PULL_REQUEST_TEMPLATE.md @@ -10,14 +10,15 @@ Remember that PRs should be made against the dev branch, unless you're preparing Learn more about contributing: [CONTRIBUTING.md](https://github.com/nf-core/bacass/tree/master/.github/CONTRIBUTING.md) --> + ## PR checklist - [ ] This comment contains a description of changes (with reason). - [ ] If you've fixed a bug or added code that should be tested, add tests! - - [ ] If you've added a new tool - add to the software_versions process and a regex to `scrape_software_versions.py` - - [ ] If you've added a new tool - have you followed the pipeline conventions in the [contribution docs](https://github.com/nf-core/bacass/tree/master/.github/CONTRIBUTING.md) - - [ ] If necessary, also make a PR on the nf-core/bacass _branch_ on the [nf-core/test-datasets](https://github.com/nf-core/test-datasets) repository. + - [ ] If you've added a new tool - add to the software_versions process and a regex to `scrape_software_versions.py` + - [ ] If you've added a new tool - have you followed the pipeline conventions in the [contribution docs](nf-core/bacass/tree/master/.github/CONTRIBUTING.md) + - [ ] If necessary, also make a PR on the nf-core/bacass _branch_ on the [nf-core/test-datasets](https://github.com/nf-core/test-datasets) repository. - [ ] Make sure your code lints (`nf-core lint .`). - [ ] Ensure the test suite passes (`nextflow run . -profile test,docker`). - [ ] Usage Documentation in `docs/usage.md` is updated. diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index 9abb167d..e364cf00 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -8,6 +8,9 @@ on: release: types: [published] +# Uncomment if we need an edge release of Nextflow again +# env: NXF_EDGE: 1 + jobs: test: name: Run workflow tests @@ -20,7 +23,7 @@ jobs: strategy: matrix: # Nextflow versions: check pipeline minimum and current latest - nxf_ver: ['20.04.0', '21.03.0-edge'] + nxf_ver: ['20.04.0', ''] steps: - name: Check out pipeline code uses: actions/checkout@v2 diff --git a/Dockerfile b/Dockerfile index e8f9e174..14b3a320 100644 --- a/Dockerfile +++ b/Dockerfile @@ -1,4 +1,4 @@ -FROM nfcore/base:1.13.3 +FROM nfcore/base:1.14 LABEL authors="Andreas Wilm, Alexander Peltzer" \ description="Docker image containing all software requirements for the nf-core/bacass pipeline" diff --git a/README.md b/README.md index 3b807be6..fa619ede 100644 --- a/README.md +++ b/README.md @@ -19,7 +19,7 @@ The pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool ## Quick Start -1. Install [`nextflow`](https://nf-co.re/usage/installation) +1. Install [`nextflow`](https://nf-co.re/usage/installation) (`>=20.04.0`) 2. Install any of [`Docker`](https://docs.docker.com/engine/installation/), [`Singularity`](https://www.sylabs.io/guides/3.0/user-guide/), [`Podman`](https://podman.io/), [`Shifter`](https://nersc.gitlab.io/development/shifter/how-to-use/) or [`Charliecloud`](https://hpc.github.io/charliecloud/) for full pipeline reproducibility _(please only use [`Conda`](https://conda.io/miniconda.html) as a last resort; see [docs](https://nf-co.re/usage/configuration#basic-configuration-profiles))_ diff --git a/lib/NfcoreSchema.groovy b/lib/NfcoreSchema.groovy index 54935ec8..52ee7304 100644 --- a/lib/NfcoreSchema.groovy +++ b/lib/NfcoreSchema.groovy @@ -112,8 +112,14 @@ class NfcoreSchema { } // unexpected params def params_ignore = params.schema_ignore_params.split(',') + 'schema_ignore_params' - if (!expectedParams.contains(specifiedParam) && !params_ignore.contains(specifiedParam)) { - unexpectedParams.push(specifiedParam) + def expectedParamsLowerCase = expectedParams.collect{ it.replace("-", "").toLowerCase() } + def specifiedParamLowerCase = specifiedParam.replace("-", "").toLowerCase() + if (!expectedParams.contains(specifiedParam) && !params_ignore.contains(specifiedParam) && !expectedParamsLowerCase.contains(specifiedParamLowerCase)) { + // Temporarily remove camelCase/camel-case params #1035 + def unexpectedParamsLowerCase = unexpectedParams.collect{ it.replace("-", "").toLowerCase()} + if (!unexpectedParamsLowerCase.contains(specifiedParamLowerCase)){ + unexpectedParams.push(specifiedParam) + } } } @@ -191,11 +197,11 @@ class NfcoreSchema { // Remove an element from a JSONArray private static JSONArray removeElement(jsonArray, element){ - def list = [] + def list = [] int len = jsonArray.length() - for (int i=0;i hnames.each { hname -> if (hostname.contains(hname) && !workflow.profile.contains(prof)) { - log.error '====================================================\n' + + log.error "${c_red}====================================================${c_reset}\n" + " ${c_red}WARNING!${c_reset} You are running with `-profile $workflow.profile`\n" + " but your machine hostname is ${c_white}'$hostname'${c_reset}\n" + " ${c_yellow_bold}It's highly recommended that you use `-profile $prof${c_reset}`\n" + - '============================================================' + "${c_red}====================================================${c_reset}\n" } } } diff --git a/nextflow.config b/nextflow.config index a3cb4f30..d3d97210 100644 --- a/nextflow.config +++ b/nextflow.config @@ -25,12 +25,13 @@ params { plaintext_email = false monochrome_logs = false help = false - igenomes_base = 's3://ngi-igenomes/igenomes/' + igenomes_base = 's3://ngi-igenomes/igenomes' tracedir = "${params.outdir}/pipeline_info" igenomes_ignore = false custom_config_version = 'master' custom_config_base = "https://raw.githubusercontent.com/nf-core/configs/${params.custom_config_version}" hostnames = false + config_profile_name = null config_profile_description = false config_profile_contact = false config_profile_url = false @@ -65,7 +66,7 @@ profiles { singularity.enabled = false podman.enabled = false shifter.enabled = false - charliecloud = false + charliecloud.enabled = false process.conda = "$projectDir/environment.yml" } debug { process.beforeScript = 'echo $HOSTNAME' } @@ -94,7 +95,7 @@ profiles { docker.enabled = false podman.enabled = true shifter.enabled = false - charliecloud = false + charliecloud.enabled = false } shifter { singularity.enabled = false @@ -129,21 +130,22 @@ env { // Capture exit codes from upstream processes when piping process.shell = ['/bin/bash', '-euo', 'pipefail'] +def trace_timestamp = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss') timeline { enabled = true - file = "${params.tracedir}/execution_timeline.html" + file = "${params.tracedir}/execution_timeline_${trace_timestamp}.html" } report { enabled = true - file = "${params.tracedir}/execution_report.html" + file = "${params.tracedir}/execution_report_${trace_timestamp}.html" } trace { enabled = true - file = "${params.tracedir}/execution_trace.txt" + file = "${params.tracedir}/execution_trace_${trace_timestamp}.txt" } dag { enabled = true - file = "${params.tracedir}/pipeline_dag.svg" + file = "${params.tracedir}/pipeline_dag_${trace_timestamp}.svg" } manifest { diff --git a/nextflow_schema.json b/nextflow_schema.json index d043e321..6b345737 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -62,7 +62,7 @@ "igenomes_base": { "type": "string", "description": "Directory / URL base for iGenomes references.", - "default": "s3://ngi-igenomes/igenomes/", + "default": "s3://ngi-igenomes/igenomes", "fa_icon": "fas fa-cloud-download-alt", "hidden": true }, @@ -183,7 +183,7 @@ "description": "Maximum amount of memory that can be requested for any single job.", "default": "128.GB", "fa_icon": "fas fa-memory", - "pattern": "^[\\d\\.]+\\s*.(K|M|G|T)?B$", + "pattern": "^\\d+(\\.\\d+)?\\.?\\s*(K|M|G|T)?B$", "hidden": true, "help_text": "Use to set an upper-limit for the memory requirement for each process. Should be a string in the format integer-unit e.g. `--max_memory '8.GB'`" }, @@ -192,7 +192,7 @@ "description": "Maximum amount of time that can be requested for any single job.", "default": "240.h", "fa_icon": "far fa-clock", - "pattern": "^[\\d\\.]+\\.*(s|m|h|d)$", + "pattern": "^(\\d+\\.?\\s*(s|m|h|day)\\s*)+$", "hidden": true, "help_text": "Use to set an upper-limit for the time requirement for each process. Should be a string in the format integer-unit e.g. `--max_time '2.h'`" } From fc85b5d9fb19c35a5721cacb9e74c16eb177f073 Mon Sep 17 00:00:00 2001 From: kevinmenden Date: Tue, 13 Jul 2021 10:37:14 +0200 Subject: [PATCH 10/81] Template update for nf-core/tools version 2.0 --- .editorconfig | 24 + .github/CONTRIBUTING.md | 2 +- .github/ISSUE_TEMPLATE/bug_report.md | 5 +- .github/PULL_REQUEST_TEMPLATE.md | 3 +- .github/workflows/awsfulltest.yml | 52 +- .github/workflows/awstest.yml | 50 +- .github/workflows/ci.yml | 19 +- .github/workflows/linting.yml | 21 +- .github/workflows/push_dockerhub_dev.yml | 28 - .github/workflows/push_dockerhub_release.yml | 29 - .gitignore | 1 - .github/markdownlint.yml => .markdownlint.yml | 2 + CITATIONS.md | 32 + Dockerfile | 13 - README.md | 71 +- assets/samplesheet.csv | 3 + assets/schema_input.json | 39 + assets/sendmail_template.txt | 36 +- bin/check_samplesheet.py | 146 +++ bin/markdown_to_html.py | 91 -- bin/scrape_software_versions.py | 44 +- conf/base.config | 94 +- conf/igenomes.config | 843 +++++++++--------- conf/modules.config | 32 + conf/test.config | 47 +- conf/test_full.config | 38 +- docs/README.md | 4 +- docs/images/mqc_fastqc_adapter.png | Bin 0 -> 23458 bytes docs/images/mqc_fastqc_counts.png | Bin 0 -> 33918 bytes docs/images/mqc_fastqc_quality.png | Bin 0 -> 55769 bytes docs/output.md | 63 +- docs/usage.md | 214 ++++- environment.yml | 15 - lib/Headers.groovy | 43 - lib/NfcoreSchema.groovy | 528 +++++------ lib/NfcoreTemplate.groovy | 266 ++++++ lib/Utils.groovy | 47 + lib/WorkflowBacass.groovy | 59 ++ lib/WorkflowMain.groovy | 94 ++ main.nf | 405 +-------- modules.json | 14 + modules/local/functions.nf | 68 ++ modules/local/get_software_versions.nf | 33 + modules/local/samplesheet_check.nf | 31 + modules/nf-core/modules/fastqc/functions.nf | 68 ++ modules/nf-core/modules/fastqc/main.nf | 47 + modules/nf-core/modules/fastqc/meta.yml | 51 ++ modules/nf-core/modules/multiqc/functions.nf | 68 ++ modules/nf-core/modules/multiqc/main.nf | 35 + modules/nf-core/modules/multiqc/meta.yml | 39 + nextflow.config | 300 ++++--- nextflow_schema.json | 255 +++--- subworkflows/local/input_check.nf | 42 + workflows/bacass.nf | 141 +++ 54 files changed, 2811 insertions(+), 1884 deletions(-) create mode 100644 .editorconfig delete mode 100644 .github/workflows/push_dockerhub_dev.yml delete mode 100644 .github/workflows/push_dockerhub_release.yml rename .github/markdownlint.yml => .markdownlint.yml (90%) create mode 100644 CITATIONS.md delete mode 100644 Dockerfile create mode 100644 assets/samplesheet.csv create mode 100644 assets/schema_input.json create mode 100755 bin/check_samplesheet.py delete mode 100755 bin/markdown_to_html.py create mode 100644 conf/modules.config create mode 100755 docs/images/mqc_fastqc_adapter.png create mode 100755 docs/images/mqc_fastqc_counts.png create mode 100755 docs/images/mqc_fastqc_quality.png delete mode 100644 environment.yml delete mode 100644 lib/Headers.groovy mode change 100644 => 100755 lib/NfcoreSchema.groovy create mode 100755 lib/NfcoreTemplate.groovy create mode 100755 lib/Utils.groovy create mode 100755 lib/WorkflowBacass.groovy create mode 100755 lib/WorkflowMain.groovy create mode 100644 modules.json create mode 100644 modules/local/functions.nf create mode 100644 modules/local/get_software_versions.nf create mode 100644 modules/local/samplesheet_check.nf create mode 100644 modules/nf-core/modules/fastqc/functions.nf create mode 100644 modules/nf-core/modules/fastqc/main.nf create mode 100644 modules/nf-core/modules/fastqc/meta.yml create mode 100644 modules/nf-core/modules/multiqc/functions.nf create mode 100644 modules/nf-core/modules/multiqc/main.nf create mode 100644 modules/nf-core/modules/multiqc/meta.yml create mode 100644 subworkflows/local/input_check.nf create mode 100644 workflows/bacass.nf diff --git a/.editorconfig b/.editorconfig new file mode 100644 index 00000000..afb20bb1 --- /dev/null +++ b/.editorconfig @@ -0,0 +1,24 @@ +root = true + +[*] +charset = utf-8 +end_of_line = lf +insert_final_newline = true +trim_trailing_whitespace = true +indent_size = 4 +indent_style = space + +[*.{yml,yaml}] +indent_size = 2 + +# These files are edited and tested upstream in nf-core/modules +[/modules/nf-core/**] +charset = unset +end_of_line = unset +insert_final_newline = unset +trim_trailing_whitespace = unset +indent_style = unset +indent_size = unset + +[/assets/email*] +indent_size = unset diff --git a/.github/CONTRIBUTING.md b/.github/CONTRIBUTING.md index 73d2e7b0..af1c3c5b 100644 --- a/.github/CONTRIBUTING.md +++ b/.github/CONTRIBUTING.md @@ -74,7 +74,7 @@ If you wish to contribute a new step, please use the following coding standards: 7. Add sanity checks for all relevant parameters. 8. Add any new software to the `scrape_software_versions.py` script in `bin/` and the version command to the `scrape_software_versions` process in `main.nf`. 9. Do local tests that the new code works properly and as expected. -10. Add a new test command in `.github/workflow/ci.yaml`. +10. Add a new test command in `.github/workflow/ci.yml`. 11. If applicable add a [MultiQC](https://https://multiqc.info/) module. 12. Update MultiQC config `assets/multiqc_config.yaml` so relevant suffixes, name clean up, General Statistics Table column order, and module figures are in the right order. 13. Optional: Add any descriptions of MultiQC report sections and output files to `docs/output.md`. diff --git a/.github/ISSUE_TEMPLATE/bug_report.md b/.github/ISSUE_TEMPLATE/bug_report.md index d6c86ac5..8e0a6155 100644 --- a/.github/ISSUE_TEMPLATE/bug_report.md +++ b/.github/ISSUE_TEMPLATE/bug_report.md @@ -18,7 +18,7 @@ Please delete this text and anything that's not relevant from the template below I have checked the following places for your error: - [ ] [nf-core website: troubleshooting](https://nf-co.re/usage/troubleshooting) -- [ ] [nf-core/bacass pipeline documentation](https://nf-co.re/nf-core/bacass/usage) +- [ ] [nf-core/bacass pipeline documentation](https://nf-co.re/bacass/usage) ## Description of the bug @@ -51,13 +51,12 @@ Have you provided the following extra information/files: ## Nextflow Installation -- Version: +- Version: ## Container engine - Engine: - version: -- Image tag: ## Additional context diff --git a/.github/PULL_REQUEST_TEMPLATE.md b/.github/PULL_REQUEST_TEMPLATE.md index bac96797..dc75e978 100644 --- a/.github/PULL_REQUEST_TEMPLATE.md +++ b/.github/PULL_REQUEST_TEMPLATE.md @@ -16,8 +16,7 @@ Learn more about contributing: [CONTRIBUTING.md](https://github.com/nf-core/baca - [ ] This comment contains a description of changes (with reason). - [ ] If you've fixed a bug or added code that should be tested, add tests! - - [ ] If you've added a new tool - add to the software_versions process and a regex to `scrape_software_versions.py` - - [ ] If you've added a new tool - have you followed the pipeline conventions in the [contribution docs](nf-core/bacass/tree/master/.github/CONTRIBUTING.md) + - [ ] If you've added a new tool - have you followed the pipeline conventions in the [contribution docs](https://github.com/nf-core/bacass/tree/master/.github/CONTRIBUTING.md) - [ ] If necessary, also make a PR on the nf-core/bacass _branch_ on the [nf-core/test-datasets](https://github.com/nf-core/test-datasets) repository. - [ ] Make sure your code lints (`nf-core lint .`). - [ ] Ensure the test suite passes (`nextflow run . -profile test,docker`). diff --git a/.github/workflows/awsfulltest.yml b/.github/workflows/awsfulltest.yml index 3314a099..dac0a97d 100644 --- a/.github/workflows/awsfulltest.yml +++ b/.github/workflows/awsfulltest.yml @@ -1,46 +1,34 @@ name: nf-core AWS full size tests # This workflow is triggered on published releases. -# It can be additionally triggered manually with GitHub actions workflow dispatch. +# It can be additionally triggered manually with GitHub actions workflow dispatch button. # It runs the -profile 'test_full' on AWS batch on: - workflow_run: - workflows: ["nf-core Docker push (release)"] - types: [completed] + release: + types: [published] workflow_dispatch: - - -env: - AWS_ACCESS_KEY_ID: ${{ secrets.AWS_ACCESS_KEY_ID }} - AWS_SECRET_ACCESS_KEY: ${{ secrets.AWS_SECRET_ACCESS_KEY }} - TOWER_ACCESS_TOKEN: ${{ secrets.AWS_TOWER_TOKEN }} - AWS_JOB_DEFINITION: ${{ secrets.AWS_JOB_DEFINITION }} - AWS_JOB_QUEUE: ${{ secrets.AWS_JOB_QUEUE }} - AWS_S3_BUCKET: ${{ secrets.AWS_S3_BUCKET }} - - jobs: - run-awstest: + run-tower: name: Run AWS full tests if: github.repository == 'nf-core/bacass' runs-on: ubuntu-latest steps: - - name: Setup Miniconda - uses: conda-incubator/setup-miniconda@v2 - with: - auto-update-conda: true - python-version: 3.7 - - name: Install awscli - run: conda install -c conda-forge awscli - - name: Start AWS batch job + - name: Launch workflow via tower + uses: nf-core/tower-action@master # TODO nf-core: You can customise AWS full pipeline tests as required # Add full size test data (but still relatively small datasets for few samples) # on the `test_full.config` test runs with only one set of parameters - # Then specify `-profile test_full` instead of `-profile test` on the AWS batch command - run: | - aws batch submit-job \ - --region eu-west-1 \ - --job-name nf-core-bacass \ - --job-queue $AWS_JOB_QUEUE \ - --job-definition $AWS_JOB_DEFINITION \ - --container-overrides '{"command": ["nf-core/bacass", "-r '"${GITHUB_SHA}"' -profile test --outdir s3://'"${AWS_S3_BUCKET}"'/bacass/results-'"${GITHUB_SHA}"' -w s3://'"${AWS_S3_BUCKET}"'/bacass/work-'"${GITHUB_SHA}"' -with-tower"], "environment": [{"name": "TOWER_ACCESS_TOKEN", "value": "'"$TOWER_ACCESS_TOKEN"'"}]}' + + with: + workspace_id: ${{ secrets.TOWER_WORKSPACE_ID }} + bearer_token: ${{ secrets.TOWER_BEARER_TOKEN }} + compute_env: ${{ secrets.TOWER_COMPUTE_ENV }} + pipeline: ${{ github.repository }} + revision: ${{ github.sha }} + workdir: s3://${{ secrets.AWS_S3_BUCKET }}/work/bacass/work-${{ github.sha }} + parameters: | + { + "outdir": "s3://${{ secrets.AWS_S3_BUCKET }}/bacass/results-${{ github.sha }}" + } + profiles: '[ "test_full", "aws_tower" ]' + diff --git a/.github/workflows/awstest.yml b/.github/workflows/awstest.yml index c61b647f..56b6ad8a 100644 --- a/.github/workflows/awstest.yml +++ b/.github/workflows/awstest.yml @@ -1,42 +1,28 @@ name: nf-core AWS test -# This workflow is triggered on push to the master branch. -# It can be additionally triggered manually with GitHub actions workflow dispatch. -# It runs the -profile 'test' on AWS batch. +# This workflow can be triggered manually with the GitHub actions workflow dispatch button. +# It runs the -profile 'test' on AWS batch on: workflow_dispatch: - - -env: - AWS_ACCESS_KEY_ID: ${{ secrets.AWS_ACCESS_KEY_ID }} - AWS_SECRET_ACCESS_KEY: ${{ secrets.AWS_SECRET_ACCESS_KEY }} - TOWER_ACCESS_TOKEN: ${{ secrets.AWS_TOWER_TOKEN }} - AWS_JOB_DEFINITION: ${{ secrets.AWS_JOB_DEFINITION }} - AWS_JOB_QUEUE: ${{ secrets.AWS_JOB_QUEUE }} - AWS_S3_BUCKET: ${{ secrets.AWS_S3_BUCKET }} - - jobs: - run-awstest: + run-tower: name: Run AWS tests if: github.repository == 'nf-core/bacass' runs-on: ubuntu-latest steps: - - name: Setup Miniconda - uses: conda-incubator/setup-miniconda@v2 + - name: Launch workflow via tower + uses: nf-core/tower-action@master + with: - auto-update-conda: true - python-version: 3.7 - - name: Install awscli - run: conda install -c conda-forge awscli - - name: Start AWS batch job - # TODO nf-core: You can customise CI pipeline run tests as required - # For example: adding multiple test runs with different parameters - # Remember that you can parallelise this by using strategy.matrix - run: | - aws batch submit-job \ - --region eu-west-1 \ - --job-name nf-core-bacass \ - --job-queue $AWS_JOB_QUEUE \ - --job-definition $AWS_JOB_DEFINITION \ - --container-overrides '{"command": ["nf-core/bacass", "-r '"${GITHUB_SHA}"' -profile test --outdir s3://'"${AWS_S3_BUCKET}"'/bacass/results-'"${GITHUB_SHA}"' -w s3://'"${AWS_S3_BUCKET}"'/bacass/work-'"${GITHUB_SHA}"' -with-tower"], "environment": [{"name": "TOWER_ACCESS_TOKEN", "value": "'"$TOWER_ACCESS_TOKEN"'"}]}' + workspace_id: ${{ secrets.TOWER_WORKSPACE_ID }} + bearer_token: ${{ secrets.TOWER_BEARER_TOKEN }} + compute_env: ${{ secrets.TOWER_COMPUTE_ENV }} + pipeline: ${{ github.repository }} + revision: ${{ github.sha }} + workdir: s3://${{ secrets.AWS_S3_BUCKET }}/work/bacass/work-${{ github.sha }} + parameters: | + { + "outdir": "s3://${{ secrets.AWS_S3_BUCKET }}/bacass/results-${{ github.sha }}" + } + profiles: '[ "test", "aws_tower" ]' + diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index e364cf00..ef7bf59f 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -23,28 +23,11 @@ jobs: strategy: matrix: # Nextflow versions: check pipeline minimum and current latest - nxf_ver: ['20.04.0', ''] + nxf_ver: ['21.04.0', ''] steps: - name: Check out pipeline code uses: actions/checkout@v2 - - name: Check if Dockerfile or Conda environment changed - uses: technote-space/get-diff-action@v4 - with: - FILES: | - Dockerfile - environment.yml - - - name: Build new docker image - if: env.MATCHED_FILES - run: docker build --no-cache . -t nfcore/bacass:dev - - - name: Pull docker image - if: ${{ !env.MATCHED_FILES }} - run: | - docker pull nfcore/bacass:dev - docker tag nfcore/bacass:dev nfcore/bacass:dev - - name: Install Nextflow env: CAPSULE_LOG: none diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml index fcde400c..13b4fc81 100644 --- a/.github/workflows/linting.yml +++ b/.github/workflows/linting.yml @@ -18,7 +18,7 @@ jobs: - name: Install markdownlint run: npm install -g markdownlint-cli - name: Run Markdownlint - run: markdownlint ${GITHUB_WORKSPACE} -c ${GITHUB_WORKSPACE}/.github/markdownlint.yml + run: markdownlint . # If the above check failed, post a comment on the PR explaining the failure - name: Post PR comment @@ -35,8 +35,8 @@ jobs: * On Mac: `brew install markdownlint-cli` * Everything else: [Install `npm`](https://www.npmjs.com/get-npm) then [install `markdownlint-cli`](https://www.npmjs.com/package/markdownlint-cli) (`npm install -g markdownlint-cli`) * Fix the markdown errors - * Automatically: `markdownlint . --config .github/markdownlint.yml --fix` - * Manually resolve anything left from `markdownlint . --config .github/markdownlint.yml` + * Automatically: `markdownlint . --fix` + * Manually resolve anything left from `markdownlint .` Once you push these changes the test should pass, and you can hide this comment :+1: @@ -46,6 +46,20 @@ jobs: repo-token: ${{ secrets.GITHUB_TOKEN }} allow-repeats: false + EditorConfig: + runs-on: ubuntu-latest + steps: + - uses: actions/checkout@v2 + + - uses: actions/setup-node@v1 + with: + node-version: "10" + + - name: Install editorconfig-checker + run: npm install -g editorconfig-checker + + - name: Run ECLint check + run: editorconfig-checker -exclude README.md $(git ls-files | grep -v test) YAML: runs-on: ubuntu-latest @@ -84,7 +98,6 @@ jobs: repo-token: ${{ secrets.GITHUB_TOKEN }} allow-repeats: false - nf-core: runs-on: ubuntu-latest steps: diff --git a/.github/workflows/push_dockerhub_dev.yml b/.github/workflows/push_dockerhub_dev.yml deleted file mode 100644 index 4403af5d..00000000 --- a/.github/workflows/push_dockerhub_dev.yml +++ /dev/null @@ -1,28 +0,0 @@ -name: nf-core Docker push (dev) -# This builds the docker image and pushes it to DockerHub -# Runs on nf-core repo releases and push event to 'dev' branch (PR merges) -on: - push: - branches: - - dev - -jobs: - push_dockerhub: - name: Push new Docker image to Docker Hub (dev) - runs-on: ubuntu-latest - # Only run for the nf-core repo, for releases and merged PRs - if: ${{ github.repository == 'nf-core/bacass' }} - env: - DOCKERHUB_USERNAME: ${{ secrets.DOCKERHUB_USERNAME }} - DOCKERHUB_PASS: ${{ secrets.DOCKERHUB_PASS }} - steps: - - name: Check out pipeline code - uses: actions/checkout@v2 - - - name: Build new docker image - run: docker build --no-cache . -t nfcore/bacass:dev - - - name: Push Docker image to DockerHub (dev) - run: | - echo "$DOCKERHUB_PASS" | docker login -u "$DOCKERHUB_USERNAME" --password-stdin - docker push nfcore/bacass:dev diff --git a/.github/workflows/push_dockerhub_release.yml b/.github/workflows/push_dockerhub_release.yml deleted file mode 100644 index 79a064c8..00000000 --- a/.github/workflows/push_dockerhub_release.yml +++ /dev/null @@ -1,29 +0,0 @@ -name: nf-core Docker push (release) -# This builds the docker image and pushes it to DockerHub -# Runs on nf-core repo releases and push event to 'dev' branch (PR merges) -on: - release: - types: [published] - -jobs: - push_dockerhub: - name: Push new Docker image to Docker Hub (release) - runs-on: ubuntu-latest - # Only run for the nf-core repo, for releases and merged PRs - if: ${{ github.repository == 'nf-core/bacass' }} - env: - DOCKERHUB_USERNAME: ${{ secrets.DOCKERHUB_USERNAME }} - DOCKERHUB_PASS: ${{ secrets.DOCKERHUB_PASS }} - steps: - - name: Check out pipeline code - uses: actions/checkout@v2 - - - name: Build new docker image - run: docker build --no-cache . -t nfcore/bacass:latest - - - name: Push Docker image to DockerHub (release) - run: | - echo "$DOCKERHUB_PASS" | docker login -u "$DOCKERHUB_USERNAME" --password-stdin - docker push nfcore/bacass:latest - docker tag nfcore/bacass:latest nfcore/bacass:${{ github.event.release.tag_name }} - docker push nfcore/bacass:${{ github.event.release.tag_name }} diff --git a/.gitignore b/.gitignore index aa4bb5b3..5124c9ac 100644 --- a/.gitignore +++ b/.gitignore @@ -3,7 +3,6 @@ work/ data/ results/ .DS_Store -tests/ testing/ testing* *.pyc diff --git a/.github/markdownlint.yml b/.markdownlint.yml similarity index 90% rename from .github/markdownlint.yml rename to .markdownlint.yml index 8d7eb53b..9e605fcf 100644 --- a/.github/markdownlint.yml +++ b/.markdownlint.yml @@ -1,6 +1,8 @@ # Markdownlint configuration file default: true line-length: false +ul-indent: + indent: 4 no-duplicate-header: siblings_only: true no-inline-html: diff --git a/CITATIONS.md b/CITATIONS.md new file mode 100644 index 00000000..6f36eb1e --- /dev/null +++ b/CITATIONS.md @@ -0,0 +1,32 @@ +# nf-core/bacass: Citations + +## [nf-core](https://pubmed.ncbi.nlm.nih.gov/32055031/) + +> Ewels PA, Peltzer A, Fillinger S, Patel H, Alneberg J, Wilm A, Garcia MU, Di Tommaso P, Nahnsen S. The nf-core framework for community-curated bioinformatics pipelines. Nat Biotechnol. 2020 Mar;38(3):276-278. doi: 10.1038/s41587-020-0439-x. PubMed PMID: 32055031. + +## [Nextflow](https://pubmed.ncbi.nlm.nih.gov/28398311/) + +> Di Tommaso P, Chatzou M, Floden EW, Barja PP, Palumbo E, Notredame C. Nextflow enables reproducible computational workflows. Nat Biotechnol. 2017 Apr 11;35(4):316-319. doi: 10.1038/nbt.3820. PubMed PMID: 28398311. + +## Pipeline tools + +* [FastQC](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/) + +* [MultiQC](https://www.ncbi.nlm.nih.gov/pubmed/27312411/) + > Ewels P, Magnusson M, Lundin S, Käller M. MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics. 2016 Oct 1;32(19):3047-8. doi: 10.1093/bioinformatics/btw354. Epub 2016 Jun 16. PubMed PMID: 27312411; PubMed Central PMCID: PMC5039924. + +## Software packaging/containerisation tools + +* [Anaconda](https://anaconda.com) + > Anaconda Software Distribution. Computer software. Vers. 2-2.4.0. Anaconda, Nov. 2016. Web. + +* [Bioconda](https://pubmed.ncbi.nlm.nih.gov/29967506/) + > Grüning B, Dale R, Sjödin A, Chapman BA, Rowe J, Tomkins-Tinch CH, Valieris R, Köster J; Bioconda Team. Bioconda: sustainable and comprehensive software distribution for the life sciences. Nat Methods. 2018 Jul;15(7):475-476. doi: 10.1038/s41592-018-0046-7. PubMed PMID: 29967506. + +* [BioContainers](https://pubmed.ncbi.nlm.nih.gov/28379341/) + > da Veiga Leprevost F, Grüning B, Aflitos SA, Röst HL, Uszkoreit J, Barsnes H, Vaudel M, Moreno P, Gatto L, Weber J, Bai M, Jimenez RC, Sachsenberg T, Pfeuffer J, Alvarez RV, Griss J, Nesvizhskii AI, Perez-Riverol Y. BioContainers: an open-source and community-driven framework for software standardization. Bioinformatics. 2017 Aug 15;33(16):2580-2582. doi: 10.1093/bioinformatics/btx192. PubMed PMID: 28379341; PubMed Central PMCID: PMC5870671. + +* [Docker](https://dl.acm.org/doi/10.5555/2600239.2600241) + +* [Singularity](https://pubmed.ncbi.nlm.nih.gov/28494014/) + > Kurtzer GM, Sochat V, Bauer MW. Singularity: Scientific containers for mobility of compute. PLoS One. 2017 May 11;12(5):e0177459. doi: 10.1371/journal.pone.0177459. eCollection 2017. PubMed PMID: 28494014; PubMed Central PMCID: PMC5426675. diff --git a/Dockerfile b/Dockerfile deleted file mode 100644 index 14b3a320..00000000 --- a/Dockerfile +++ /dev/null @@ -1,13 +0,0 @@ -FROM nfcore/base:1.14 -LABEL authors="Andreas Wilm, Alexander Peltzer" \ - description="Docker image containing all software requirements for the nf-core/bacass pipeline" - -# Install the conda environment -COPY environment.yml / -RUN conda env create --quiet -f /environment.yml && conda clean -a - -# Add conda installation dir to PATH (instead of doing 'conda activate') -ENV PATH /opt/conda/envs/nf-core-bacass-1.1.1/bin:$PATH - -# Dump the details of the installed packages to a file for posterity -RUN conda env export --name nf-core-bacass-1.1.1 > nf-core-bacass-1.1.1.yml diff --git a/README.md b/README.md index fa619ede..53353e7f 100644 --- a/README.md +++ b/README.md @@ -1,67 +1,69 @@ # ![nf-core/bacass](docs/images/nf-core-bacass_logo.png) -**Simple bacterial assembly and annotation pipeline.**. +[![GitHub Actions CI Status](https://github.com/nf-core/bacass/workflows/nf-core%20CI/badge.svg)](https://github.com/nf-core/bacass/actions?query=workflow%3A%22nf-core+CI%22) +[![GitHub Actions Linting Status](https://github.com/nf-core/bacass/workflows/nf-core%20linting/badge.svg)](https://github.com/nf-core/bacass/actions?query=workflow%3A%22nf-core+linting%22) +[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/bacass/results) +[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX) -[![GitHub Actions CI Status](https://github.com/nf-core/bacass/workflows/nf-core%20CI/badge.svg)](https://github.com/nf-core/bacass/actions) -[![GitHub Actions Linting Status](https://github.com/nf-core/bacass/workflows/nf-core%20linting/badge.svg)](https://github.com/nf-core/bacass/actions) -[![Nextflow](https://img.shields.io/badge/nextflow-%E2%89%A520.04.0-brightgreen.svg)](https://www.nextflow.io/) +[![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A521.04.0-23aa62.svg?labelColor=000000)](https://www.nextflow.io/) +[![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/) +[![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/) +[![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/) -[![install with bioconda](https://img.shields.io/badge/install%20with-bioconda-brightgreen.svg)](https://bioconda.github.io/) -[![Docker](https://img.shields.io/docker/automated/nfcore/bacass.svg)](https://hub.docker.com/r/nfcore/bacass) -[![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23bacass-4A154B?logo=slack)](https://nfcore.slack.com/channels/bacass) +[![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23bacass-4A154B?labelColor=000000&logo=slack)](https://nfcore.slack.com/channels/bacass) +[![Follow on Twitter](http://img.shields.io/badge/twitter-%40nf__core-1DA1F2?labelColor=000000&logo=twitter)](https://twitter.com/nf_core) +[![Watch on YouTube](http://img.shields.io/badge/youtube-nf--core-FF0000?labelColor=000000&logo=youtube)](https://www.youtube.com/c/nf-core) ## Introduction -**nf-core/bacass** is a bioinformatics best-practise analysis pipeline for +**nf-core/bacass** is a bioinformatics best-practice analysis pipeline for Simple bacterial assembly and annotation pipeline.. -The pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool to run tasks across multiple compute infrastructures in a very portable manner. It comes with docker containers making installation trivial and results highly reproducible. +The pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool to run tasks across multiple compute infrastructures in a very portable manner. It uses Docker/Singularity containers making installation trivial and results highly reproducible. The [Nextflow DSL2](https://www.nextflow.io/docs/latest/dsl2.html) implementation of this pipeline uses one container per process which makes it much easier to maintain and update software dependencies. Where possible, these processes have been submitted to and installed from [nf-core/modules](https://github.com/nf-core/modules) in order to make them available to all nf-core pipelines, and to everyone within the Nextflow community! + + +On release, automated continuous integration tests run the pipeline on a full-sized dataset on the AWS cloud infrastructure. This ensures that the pipeline runs on AWS, has sensible resource allocation defaults set to run on real-world datasets, and permits the persistent storage of results to benchmark between pipeline releases and other analysis sources. The results obtained from the full-sized test can be viewed on the [nf-core website](https://nf-co.re/bacass/results). + +## Pipeline summary + + + +1. Read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/)) +2. Present QC for raw reads ([`MultiQC`](http://multiqc.info/)) ## Quick Start -1. Install [`nextflow`](https://nf-co.re/usage/installation) (`>=20.04.0`) +1. Install [`Nextflow`](https://nf-co.re/usage/installation) (`>=21.04.0`) 2. Install any of [`Docker`](https://docs.docker.com/engine/installation/), [`Singularity`](https://www.sylabs.io/guides/3.0/user-guide/), [`Podman`](https://podman.io/), [`Shifter`](https://nersc.gitlab.io/development/shifter/how-to-use/) or [`Charliecloud`](https://hpc.github.io/charliecloud/) for full pipeline reproducibility _(please only use [`Conda`](https://conda.io/miniconda.html) as a last resort; see [docs](https://nf-co.re/usage/configuration#basic-configuration-profiles))_ 3. Download the pipeline and test it on a minimal dataset with a single command: - ```bash + ```console nextflow run nf-core/bacass -profile test, ``` - > Please check [nf-core/configs](https://github.com/nf-core/configs#documentation) to see if a custom config file to run nf-core pipelines already exists for your Institute. If so, you can simply use `-profile ` in your command. This will enable either `docker` or `singularity` and set the appropriate execution settings for your local compute environment. + > * Please check [nf-core/configs](https://github.com/nf-core/configs#documentation) to see if a custom config file to run nf-core pipelines already exists for your Institute. If so, you can simply use `-profile ` in your command. This will enable either `docker` or `singularity` and set the appropriate execution settings for your local compute environment. + > * If you are using `singularity` then the pipeline will auto-detect this and attempt to download the Singularity images directly as opposed to performing a conversion from Docker images. If you are persistently observing issues downloading Singularity images directly due to timeout or network issues then please use the `--singularity_pull_docker_container` parameter to pull and convert the Docker image instead. Alternatively, it is highly recommended to use the [`nf-core download`](https://nf-co.re/tools/#downloading-pipelines-for-offline-use) command to pre-download all of the required containers before running the pipeline and to set the [`NXF_SINGULARITY_CACHEDIR` or `singularity.cacheDir`](https://www.nextflow.io/docs/latest/singularity.html?#singularity-docker-hub) Nextflow options to be able to store and re-use the images from a central location for future pipeline runs. + > * If you are using `conda`, it is highly recommended to use the [`NXF_CONDA_CACHEDIR` or `conda.cacheDir`](https://www.nextflow.io/docs/latest/conda.html) settings to store the environments in a central location for future pipeline runs. 4. Start running your own analysis! - ```bash - nextflow run nf-core/bacass -profile --input '*_R{1,2}.fastq.gz' --genome GRCh37 + ```console + nextflow run nf-core/bacass -profile --input samplesheet.csv --genome GRCh37 ``` -See [usage docs](https://nf-co.re/bacass/usage) for all of the available options when running the pipeline. - -## Pipeline Summary - -By default, the pipeline currently performs the following: - - - -* Sequencing quality control (`FastQC`) -* Overall pipeline run summaries (`MultiQC`) - ## Documentation -The nf-core/bacass pipeline comes with documentation about the pipeline: [usage](https://nf-co.re/bacass/usage) and [output](https://nf-co.re/bacass/output). - - +The nf-core/bacass pipeline comes with documentation about the pipeline [usage](https://nf-co.re/bacass/usage), [parameters](https://nf-co.re/bacass/parameters) and [output](https://nf-co.re/bacass/output). ## Credits nf-core/bacass was originally written by Andreas Wilm, Alexander Peltzer. -We thank the following people for their extensive assistance in the development -of this pipeline: +We thank the following people for their extensive assistance in the development of this pipeline: @@ -73,9 +75,12 @@ For further information or help, don't hesitate to get in touch on the [Slack `# ## Citations - + + +An extensive list of references for the tools used by the pipeline can be found in the [`CITATIONS.md`](CITATIONS.md) file. + You can cite the `nf-core` publication as follows: > **The nf-core framework for community-curated bioinformatics pipelines.** @@ -83,7 +88,3 @@ You can cite the `nf-core` publication as follows: > Philip Ewels, Alexander Peltzer, Sven Fillinger, Harshil Patel, Johannes Alneberg, Andreas Wilm, Maxime Ulysse Garcia, Paolo Di Tommaso & Sven Nahnsen. > > _Nat Biotechnol._ 2020 Feb 13. doi: [10.1038/s41587-020-0439-x](https://dx.doi.org/10.1038/s41587-020-0439-x). - -In addition, references of tools and data used in this pipeline are as follows: - - diff --git a/assets/samplesheet.csv b/assets/samplesheet.csv new file mode 100644 index 00000000..5f653ab7 --- /dev/null +++ b/assets/samplesheet.csv @@ -0,0 +1,3 @@ +sample,fastq_1,fastq_2 +SAMPLE_PAIRED_END,/path/to/fastq/files/AEG588A1_S1_L002_R1_001.fastq.gz,/path/to/fastq/files/AEG588A1_S1_L002_R2_001.fastq.gz +SAMPLE_SINGLE_END,/path/to/fastq/files/AEG588A4_S4_L003_R1_001.fastq.gz, diff --git a/assets/schema_input.json b/assets/schema_input.json new file mode 100644 index 00000000..9d286b07 --- /dev/null +++ b/assets/schema_input.json @@ -0,0 +1,39 @@ +{ + "$schema": "http://json-schema.org/draft-07/schema", + "$id": "https://raw.githubusercontent.com/nf-core/bacass/master/assets/schema_input.json", + "title": "nf-core/bacass pipeline - params.input schema", + "description": "Schema for the file provided with params.input", + "type": "array", + "items": { + "type": "object", + "properties": { + "sample": { + "type": "string", + "pattern": "^\\S+$", + "errorMessage": "Sample name must be provided and cannot contain spaces" + }, + "fastq_1": { + "type": "string", + "pattern": "^\\S+\\.f(ast)?q\\.gz$", + "errorMessage": "FastQ file for reads 1 must be provided, cannot contain spaces and must have extension '.fq.gz' or '.fastq.gz'" + }, + "fastq_2": { + "errorMessage": "FastQ file for reads 2 cannot contain spaces and must have extension '.fq.gz' or '.fastq.gz'", + "anyOf": [ + { + "type": "string", + "pattern": "^\\S+\\.f(ast)?q\\.gz$" + }, + { + "type": "string", + "maxLength": 0 + } + ] + } + }, + "required": [ + "sample", + "fastq_1" + ] + } +} diff --git a/assets/sendmail_template.txt b/assets/sendmail_template.txt index 71d5412e..8f0cfd4c 100644 --- a/assets/sendmail_template.txt +++ b/assets/sendmail_template.txt @@ -15,15 +15,15 @@ Content-ID: Content-Disposition: inline; filename="nf-core-bacass_logo.png" <% out << new File("$projectDir/assets/nf-core-bacass_logo.png"). - bytes. - encodeBase64(). - toString(). - tokenize( '\n' )*. - toList()*. - collate( 76 )*. - collect { it.join() }. - flatten(). - join( '\n' ) %> + bytes. + encodeBase64(). + toString(). + tokenize( '\n' )*. + toList()*. + collate( 76 )*. + collect { it.join() }. + flatten(). + join( '\n' ) %> <% if (mqcFile){ @@ -37,15 +37,15 @@ Content-ID: Content-Disposition: attachment; filename=\"${mqcFileObj.getName()}\" ${mqcFileObj. - bytes. - encodeBase64(). - toString(). - tokenize( '\n' )*. - toList()*. - collate( 76 )*. - collect { it.join() }. - flatten(). - join( '\n' )} + bytes. + encodeBase64(). + toString(). + tokenize( '\n' )*. + toList()*. + collate( 76 )*. + collect { it.join() }. + flatten(). + join( '\n' )} """ }} %> diff --git a/bin/check_samplesheet.py b/bin/check_samplesheet.py new file mode 100755 index 00000000..cef3e4fe --- /dev/null +++ b/bin/check_samplesheet.py @@ -0,0 +1,146 @@ +#!/usr/bin/env python + +# TODO nf-core: Update the script to check the samplesheet +# This script is based on the example at: https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv + +import os +import sys +import errno +import argparse + + +def parse_args(args=None): + Description = "Reformat nf-core/bacass samplesheet file and check its contents." + Epilog = "Example usage: python check_samplesheet.py " + + parser = argparse.ArgumentParser(description=Description, epilog=Epilog) + parser.add_argument("FILE_IN", help="Input samplesheet file.") + parser.add_argument("FILE_OUT", help="Output file.") + return parser.parse_args(args) + + +def make_dir(path): + if len(path) > 0: + try: + os.makedirs(path) + except OSError as exception: + if exception.errno != errno.EEXIST: + raise exception + + +def print_error(error, context="Line", context_str=""): + error_str = "ERROR: Please check samplesheet -> {}".format(error) + if context != "" and context_str != "": + error_str = "ERROR: Please check samplesheet -> {}\n{}: '{}'".format( + error, context.strip(), context_str.strip() + ) + print(error_str) + sys.exit(1) + + +# TODO nf-core: Update the check_samplesheet function +def check_samplesheet(file_in, file_out): + """ + This function checks that the samplesheet follows the following structure: + + sample,fastq_1,fastq_2 + SAMPLE_PE,SAMPLE_PE_RUN1_1.fastq.gz,SAMPLE_PE_RUN1_2.fastq.gz + SAMPLE_PE,SAMPLE_PE_RUN2_1.fastq.gz,SAMPLE_PE_RUN2_2.fastq.gz + SAMPLE_SE,SAMPLE_SE_RUN1_1.fastq.gz, + + For an example see: + https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv + """ + + sample_mapping_dict = {} + with open(file_in, "r") as fin: + + ## Check header + MIN_COLS = 2 + # TODO nf-core: Update the column names for the input samplesheet + HEADER = ["sample", "fastq_1", "fastq_2"] + header = [x.strip('"') for x in fin.readline().strip().split(",")] + if header[: len(HEADER)] != HEADER: + print("ERROR: Please check samplesheet header -> {} != {}".format(",".join(header), ",".join(HEADER))) + sys.exit(1) + + ## Check sample entries + for line in fin: + lspl = [x.strip().strip('"') for x in line.strip().split(",")] + + # Check valid number of columns per row + if len(lspl) < len(HEADER): + print_error( + "Invalid number of columns (minimum = {})!".format(len(HEADER)), + "Line", + line, + ) + num_cols = len([x for x in lspl if x]) + if num_cols < MIN_COLS: + print_error( + "Invalid number of populated columns (minimum = {})!".format(MIN_COLS), + "Line", + line, + ) + + ## Check sample name entries + sample, fastq_1, fastq_2 = lspl[: len(HEADER)] + sample = sample.replace(" ", "_") + if not sample: + print_error("Sample entry has not been specified!", "Line", line) + + ## Check FastQ file extension + for fastq in [fastq_1, fastq_2]: + if fastq: + if fastq.find(" ") != -1: + print_error("FastQ file contains spaces!", "Line", line) + if not fastq.endswith(".fastq.gz") and not fastq.endswith(".fq.gz"): + print_error( + "FastQ file does not have extension '.fastq.gz' or '.fq.gz'!", + "Line", + line, + ) + + ## Auto-detect paired-end/single-end + sample_info = [] ## [single_end, fastq_1, fastq_2] + if sample and fastq_1 and fastq_2: ## Paired-end short reads + sample_info = ["0", fastq_1, fastq_2] + elif sample and fastq_1 and not fastq_2: ## Single-end short reads + sample_info = ["1", fastq_1, fastq_2] + else: + print_error("Invalid combination of columns provided!", "Line", line) + + ## Create sample mapping dictionary = { sample: [ single_end, fastq_1, fastq_2 ] } + if sample not in sample_mapping_dict: + sample_mapping_dict[sample] = [sample_info] + else: + if sample_info in sample_mapping_dict[sample]: + print_error("Samplesheet contains duplicate rows!", "Line", line) + else: + sample_mapping_dict[sample].append(sample_info) + + ## Write validated samplesheet with appropriate columns + if len(sample_mapping_dict) > 0: + out_dir = os.path.dirname(file_out) + make_dir(out_dir) + with open(file_out, "w") as fout: + fout.write(",".join(["sample", "single_end", "fastq_1", "fastq_2"]) + "\n") + for sample in sorted(sample_mapping_dict.keys()): + + ## Check that multiple runs of the same sample are of the same datatype + if not all(x[0] == sample_mapping_dict[sample][0][0] for x in sample_mapping_dict[sample]): + print_error("Multiple runs of a sample must be of the same datatype!", "Sample: {}".format(sample)) + + for idx, val in enumerate(sample_mapping_dict[sample]): + fout.write(",".join(["{}_T{}".format(sample, idx + 1)] + val) + "\n") + else: + print_error("No entries to process!", "Samplesheet: {}".format(file_in)) + + +def main(args=None): + args = parse_args(args) + check_samplesheet(args.FILE_IN, args.FILE_OUT) + + +if __name__ == "__main__": + sys.exit(main()) diff --git a/bin/markdown_to_html.py b/bin/markdown_to_html.py deleted file mode 100755 index a26d1ff5..00000000 --- a/bin/markdown_to_html.py +++ /dev/null @@ -1,91 +0,0 @@ -#!/usr/bin/env python -from __future__ import print_function -import argparse -import markdown -import os -import sys -import io - - -def convert_markdown(in_fn): - input_md = io.open(in_fn, mode="r", encoding="utf-8").read() - html = markdown.markdown( - "[TOC]\n" + input_md, - extensions=["pymdownx.extra", "pymdownx.b64", "pymdownx.highlight", "pymdownx.emoji", "pymdownx.tilde", "toc"], - extension_configs={ - "pymdownx.b64": {"base_path": os.path.dirname(in_fn)}, - "pymdownx.highlight": {"noclasses": True}, - "toc": {"title": "Table of Contents"}, - }, - ) - return html - - -def wrap_html(contents): - header = """ - - - - - -

- - - """ - return header + contents + footer - - -def parse_args(args=None): - parser = argparse.ArgumentParser() - parser.add_argument("mdfile", type=argparse.FileType("r"), nargs="?", help="File to convert. Defaults to stdin.") - parser.add_argument( - "-o", "--out", type=argparse.FileType("w"), default=sys.stdout, help="Output file name. Defaults to stdout." - ) - return parser.parse_args(args) - - -def main(args=None): - args = parse_args(args) - converted_md = convert_markdown(args.mdfile.name) - html = wrap_html(converted_md) - args.out.write(html) - - -if __name__ == "__main__": - sys.exit(main()) diff --git a/bin/scrape_software_versions.py b/bin/scrape_software_versions.py index 1ea01f65..b4dc6516 100755 --- a/bin/scrape_software_versions.py +++ b/bin/scrape_software_versions.py @@ -1,36 +1,18 @@ #!/usr/bin/env python from __future__ import print_function -from collections import OrderedDict -import re +import os -# TODO nf-core: Add additional regexes for new tools in process get_software_versions -regexes = { - "nf-core/bacass": ["v_pipeline.txt", r"(\S+)"], - "Nextflow": ["v_nextflow.txt", r"(\S+)"], - "FastQC": ["v_fastqc.txt", r"FastQC v(\S+)"], - "MultiQC": ["v_multiqc.txt", r"multiqc, version (\S+)"], -} -results = OrderedDict() -results["nf-core/bacass"] = 'N/A' -results["Nextflow"] = 'N/A' -results["FastQC"] = 'N/A' -results["MultiQC"] = 'N/A' +results = {} +version_files = [x for x in os.listdir(".") if x.endswith(".version.txt")] +for version_file in version_files: -# Search each file using its regex -for k, v in regexes.items(): - try: - with open(v[0]) as x: - versions = x.read() - match = re.search(v[1], versions) - if match: - results[k] = "v{}".format(match.group(1)) - except IOError: - results[k] = False + software = version_file.replace(".version.txt", "") + if software == "pipeline": + software = "nf-core/bacass" -# Remove software set to false in results -for k in list(results): - if not results[k]: - del results[k] + with open(version_file) as fin: + version = fin.read().strip() + results[software] = version # Dump to YAML print( @@ -44,11 +26,11 @@
""" ) -for k, v in results.items(): +for k, v in sorted(results.items()): print("
{}
{}
".format(k, v)) print("
") # Write out regexes as csv file: -with open("software_versions.csv", "w") as f: - for k, v in results.items(): +with open("software_versions.tsv", "w") as f: + for k, v in sorted(results.items()): f.write("{}\t{}\n".format(k, v)) diff --git a/conf/base.config b/conf/base.config index 79d3c26f..afa50340 100644 --- a/conf/base.config +++ b/conf/base.config @@ -1,51 +1,57 @@ /* - * ------------------------------------------------- - * nf-core/bacass Nextflow base config file - * ------------------------------------------------- - * A 'blank slate' config file, appropriate for general - * use on most high performace compute environments. - * Assumes that all software is installed and available - * on the PATH. Runs in `local` mode - all jobs will be - * run on the logged in environment. - */ +======================================================================================== + nf-core/bacass Nextflow base config file +======================================================================================== + A 'blank slate' config file, appropriate for general use on most high performance + compute environments. Assumes that all software is installed and available on + the PATH. Runs in `local` mode - all jobs will be run on the logged in environment. +---------------------------------------------------------------------------------------- +*/ process { - // TODO nf-core: Check the defaults for all processes - cpus = { check_max( 1 * task.attempt, 'cpus' ) } - memory = { check_max( 7.GB * task.attempt, 'memory' ) } - time = { check_max( 4.h * task.attempt, 'time' ) } + // TODO nf-core: Check the defaults for all processes + cpus = { check_max( 1 * task.attempt, 'cpus' ) } + memory = { check_max( 6.GB * task.attempt, 'memory' ) } + time = { check_max( 4.h * task.attempt, 'time' ) } - errorStrategy = { task.exitStatus in [143,137,104,134,139] ? 'retry' : 'finish' } - maxRetries = 1 - maxErrors = '-1' - - // Process-specific resource requirements - // NOTE - Only one of the labels below are used in the fastqc process in the main script. - // If possible, it would be nice to keep the same label naming convention when - // adding in your processes. - // TODO nf-core: Customise requirements for specific processes. - // See https://www.nextflow.io/docs/latest/config.html#config-process-selectors - withLabel:process_low { - cpus = { check_max( 2 * task.attempt, 'cpus' ) } - memory = { check_max( 14.GB * task.attempt, 'memory' ) } - time = { check_max( 6.h * task.attempt, 'time' ) } - } - withLabel:process_medium { - cpus = { check_max( 6 * task.attempt, 'cpus' ) } - memory = { check_max( 42.GB * task.attempt, 'memory' ) } - time = { check_max( 8.h * task.attempt, 'time' ) } - } - withLabel:process_high { - cpus = { check_max( 12 * task.attempt, 'cpus' ) } - memory = { check_max( 84.GB * task.attempt, 'memory' ) } - time = { check_max( 10.h * task.attempt, 'time' ) } - } - withLabel:process_long { - time = { check_max( 20.h * task.attempt, 'time' ) } - } - withName:get_software_versions { - cache = false - } + errorStrategy = { task.exitStatus in [143,137,104,134,139] ? 'retry' : 'finish' } + maxRetries = 1 + maxErrors = '-1' + // Process-specific resource requirements + // NOTE - Please try and re-use the labels below as much as possible. + // These labels are used and recognised by default in DSL2 files hosted on nf-core/modules. + // If possible, it would be nice to keep the same label naming convention when + // adding in your local modules too. + // TODO nf-core: Customise requirements for specific processes. + // See https://www.nextflow.io/docs/latest/config.html#config-process-selectors + withLabel:process_low { + cpus = { check_max( 2 * task.attempt, 'cpus' ) } + memory = { check_max( 12.GB * task.attempt, 'memory' ) } + time = { check_max( 4.h * task.attempt, 'time' ) } + } + withLabel:process_medium { + cpus = { check_max( 6 * task.attempt, 'cpus' ) } + memory = { check_max( 36.GB * task.attempt, 'memory' ) } + time = { check_max( 8.h * task.attempt, 'time' ) } + } + withLabel:process_high { + cpus = { check_max( 12 * task.attempt, 'cpus' ) } + memory = { check_max( 72.GB * task.attempt, 'memory' ) } + time = { check_max( 16.h * task.attempt, 'time' ) } + } + withLabel:process_long { + time = { check_max( 20.h * task.attempt, 'time' ) } + } + withLabel:process_high_memory { + memory = { check_max( 200.GB * task.attempt, 'memory' ) } + } + withLabel:error_ignore { + errorStrategy = 'ignore' + } + withLabel:error_retry { + errorStrategy = 'retry' + maxRetries = 2 + } } diff --git a/conf/igenomes.config b/conf/igenomes.config index 31b7ee61..855948de 100644 --- a/conf/igenomes.config +++ b/conf/igenomes.config @@ -1,421 +1,432 @@ /* - * ------------------------------------------------- - * Nextflow config file for iGenomes paths - * ------------------------------------------------- - * Defines reference genomes, using iGenome paths - * Can be used by any config that customises the base - * path using $params.igenomes_base / --igenomes_base - */ +======================================================================================== + Nextflow config file for iGenomes paths +======================================================================================== + Defines reference genomes using iGenome paths. + Can be used by any config that customises the base path using: + $params.igenomes_base / --igenomes_base +---------------------------------------------------------------------------------------- +*/ params { - // illumina iGenomes reference file paths - genomes { - 'GRCh37' { - fasta = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Annotation/README.txt" - mito_name = "MT" - macs_gsize = "2.7e9" - blacklist = "${projectDir}/assets/blacklists/GRCh37-blacklist.bed" + // illumina iGenomes reference file paths + genomes { + 'GRCh37' { + fasta = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Annotation/README.txt" + mito_name = "MT" + macs_gsize = "2.7e9" + blacklist = "${projectDir}/assets/blacklists/GRCh37-blacklist.bed" + } + 'GRCh38' { + fasta = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Annotation/Genes/genes.bed" + mito_name = "chrM" + macs_gsize = "2.7e9" + blacklist = "${projectDir}/assets/blacklists/hg38-blacklist.bed" + } + 'GRCm38' { + fasta = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Annotation/README.txt" + mito_name = "MT" + macs_gsize = "1.87e9" + blacklist = "${projectDir}/assets/blacklists/GRCm38-blacklist.bed" + } + 'TAIR10' { + fasta = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Annotation/README.txt" + mito_name = "Mt" + } + 'EB2' { + fasta = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Annotation/README.txt" + } + 'UMD3.1' { + fasta = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Annotation/README.txt" + mito_name = "MT" + } + 'WBcel235' { + fasta = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Annotation/Genes/genes.bed" + mito_name = "MtDNA" + macs_gsize = "9e7" + } + 'CanFam3.1' { + fasta = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Annotation/README.txt" + mito_name = "MT" + } + 'GRCz10' { + fasta = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Annotation/Genes/genes.bed" + mito_name = "MT" + } + 'BDGP6' { + fasta = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Annotation/Genes/genes.bed" + mito_name = "M" + macs_gsize = "1.2e8" + } + 'EquCab2' { + fasta = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Annotation/README.txt" + mito_name = "MT" + } + 'EB1' { + fasta = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Annotation/README.txt" + } + 'Galgal4' { + fasta = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Annotation/Genes/genes.bed" + mito_name = "MT" + } + 'Gm01' { + fasta = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Annotation/README.txt" + } + 'Mmul_1' { + fasta = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Annotation/README.txt" + mito_name = "MT" + } + 'IRGSP-1.0' { + fasta = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Annotation/Genes/genes.bed" + mito_name = "Mt" + } + 'CHIMP2.1.4' { + fasta = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Annotation/README.txt" + mito_name = "MT" + } + 'Rnor_5.0' { + fasta = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Annotation/Genes/genes.bed" + mito_name = "MT" + } + 'Rnor_6.0' { + fasta = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Annotation/Genes/genes.bed" + mito_name = "MT" + } + 'R64-1-1' { + fasta = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Annotation/Genes/genes.bed" + mito_name = "MT" + macs_gsize = "1.2e7" + } + 'EF2' { + fasta = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Annotation/README.txt" + mito_name = "MT" + macs_gsize = "1.21e7" + } + 'Sbi1' { + fasta = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Annotation/README.txt" + } + 'Sscrofa10.2' { + fasta = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Annotation/README.txt" + mito_name = "MT" + } + 'AGPv3' { + fasta = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Annotation/Genes/genes.bed" + mito_name = "Mt" + } + 'hg38' { + fasta = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Annotation/Genes/genes.bed" + mito_name = "chrM" + macs_gsize = "2.7e9" + blacklist = "${projectDir}/assets/blacklists/hg38-blacklist.bed" + } + 'hg19' { + fasta = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Annotation/README.txt" + mito_name = "chrM" + macs_gsize = "2.7e9" + blacklist = "${projectDir}/assets/blacklists/hg19-blacklist.bed" + } + 'mm10' { + fasta = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Annotation/README.txt" + mito_name = "chrM" + macs_gsize = "1.87e9" + blacklist = "${projectDir}/assets/blacklists/mm10-blacklist.bed" + } + 'bosTau8' { + fasta = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Annotation/Genes/genes.bed" + mito_name = "chrM" + } + 'ce10' { + fasta = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Annotation/README.txt" + mito_name = "chrM" + macs_gsize = "9e7" + } + 'canFam3' { + fasta = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Annotation/README.txt" + mito_name = "chrM" + } + 'danRer10' { + fasta = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Annotation/Genes/genes.bed" + mito_name = "chrM" + macs_gsize = "1.37e9" + } + 'dm6' { + fasta = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Annotation/Genes/genes.bed" + mito_name = "chrM" + macs_gsize = "1.2e8" + } + 'equCab2' { + fasta = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Annotation/README.txt" + mito_name = "chrM" + } + 'galGal4' { + fasta = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Annotation/README.txt" + mito_name = "chrM" + } + 'panTro4' { + fasta = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Annotation/README.txt" + mito_name = "chrM" + } + 'rn6' { + fasta = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Annotation/Genes/genes.bed" + mito_name = "chrM" + } + 'sacCer3' { + fasta = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/BismarkIndex/" + readme = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Annotation/README.txt" + mito_name = "chrM" + macs_gsize = "1.2e7" + } + 'susScr3' { + fasta = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Annotation/README.txt" + mito_name = "chrM" + } } - 'GRCh38' { - fasta = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Annotation/Genes/genes.bed" - mito_name = "chrM" - macs_gsize = "2.7e9" - blacklist = "${projectDir}/assets/blacklists/hg38-blacklist.bed" - } - 'GRCm38' { - fasta = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Annotation/README.txt" - mito_name = "MT" - macs_gsize = "1.87e9" - blacklist = "${projectDir}/assets/blacklists/GRCm38-blacklist.bed" - } - 'TAIR10' { - fasta = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Annotation/README.txt" - mito_name = "Mt" - } - 'EB2' { - fasta = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Annotation/README.txt" - } - 'UMD3.1' { - fasta = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Annotation/README.txt" - mito_name = "MT" - } - 'WBcel235' { - fasta = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Annotation/Genes/genes.bed" - mito_name = "MtDNA" - macs_gsize = "9e7" - } - 'CanFam3.1' { - fasta = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Annotation/README.txt" - mito_name = "MT" - } - 'GRCz10' { - fasta = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Annotation/Genes/genes.bed" - mito_name = "MT" - } - 'BDGP6' { - fasta = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Annotation/Genes/genes.bed" - mito_name = "M" - macs_gsize = "1.2e8" - } - 'EquCab2' { - fasta = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Annotation/README.txt" - mito_name = "MT" - } - 'EB1' { - fasta = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Annotation/README.txt" - } - 'Galgal4' { - fasta = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Annotation/Genes/genes.bed" - mito_name = "MT" - } - 'Gm01' { - fasta = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Annotation/README.txt" - } - 'Mmul_1' { - fasta = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Annotation/README.txt" - mito_name = "MT" - } - 'IRGSP-1.0' { - fasta = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Annotation/Genes/genes.bed" - mito_name = "Mt" - } - 'CHIMP2.1.4' { - fasta = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Annotation/README.txt" - mito_name = "MT" - } - 'Rnor_6.0' { - fasta = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Annotation/Genes/genes.bed" - mito_name = "MT" - } - 'R64-1-1' { - fasta = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Annotation/Genes/genes.bed" - mito_name = "MT" - macs_gsize = "1.2e7" - } - 'EF2' { - fasta = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Annotation/README.txt" - mito_name = "MT" - macs_gsize = "1.21e7" - } - 'Sbi1' { - fasta = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Annotation/README.txt" - } - 'Sscrofa10.2' { - fasta = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Annotation/README.txt" - mito_name = "MT" - } - 'AGPv3' { - fasta = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Annotation/Genes/genes.bed" - mito_name = "Mt" - } - 'hg38' { - fasta = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Annotation/Genes/genes.bed" - mito_name = "chrM" - macs_gsize = "2.7e9" - blacklist = "${projectDir}/assets/blacklists/hg38-blacklist.bed" - } - 'hg19' { - fasta = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Annotation/README.txt" - mito_name = "chrM" - macs_gsize = "2.7e9" - blacklist = "${projectDir}/assets/blacklists/hg19-blacklist.bed" - } - 'mm10' { - fasta = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Annotation/README.txt" - mito_name = "chrM" - macs_gsize = "1.87e9" - blacklist = "${projectDir}/assets/blacklists/mm10-blacklist.bed" - } - 'bosTau8' { - fasta = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Annotation/Genes/genes.bed" - mito_name = "chrM" - } - 'ce10' { - fasta = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Annotation/README.txt" - mito_name = "chrM" - macs_gsize = "9e7" - } - 'canFam3' { - fasta = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Annotation/README.txt" - mito_name = "chrM" - } - 'danRer10' { - fasta = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Annotation/Genes/genes.bed" - mito_name = "chrM" - macs_gsize = "1.37e9" - } - 'dm6' { - fasta = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Annotation/Genes/genes.bed" - mito_name = "chrM" - macs_gsize = "1.2e8" - } - 'equCab2' { - fasta = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Annotation/README.txt" - mito_name = "chrM" - } - 'galGal4' { - fasta = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Annotation/README.txt" - mito_name = "chrM" - } - 'panTro4' { - fasta = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Annotation/README.txt" - mito_name = "chrM" - } - 'rn6' { - fasta = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Annotation/Genes/genes.bed" - mito_name = "chrM" - } - 'sacCer3' { - fasta = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/BismarkIndex/" - readme = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Annotation/README.txt" - mito_name = "chrM" - macs_gsize = "1.2e7" - } - 'susScr3' { - fasta = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Annotation/README.txt" - mito_name = "chrM" - } - } } diff --git a/conf/modules.config b/conf/modules.config new file mode 100644 index 00000000..0b1bfdec --- /dev/null +++ b/conf/modules.config @@ -0,0 +1,32 @@ +/* +======================================================================================== + Config file for defining DSL2 per module options +======================================================================================== + Available keys to override module options: + args = Additional arguments appended to command in module. + args2 = Second set of arguments appended to command in module (multi-tool modules). + args3 = Third set of arguments appended to command in module (multi-tool modules). + publish_dir = Directory to publish results. + publish_by_meta = Groovy list of keys available in meta map to append as directories to "publish_dir" path + If publish_by_meta = true - Value of ${meta['id']} is appended as a directory to "publish_dir" path + If publish_by_meta = ['id', 'custompath'] - If "id" is in meta map and "custompath" isn't then "${meta['id']}/custompath/" + is appended as a directory to "publish_dir" path + If publish_by_meta = false / null - No directories are appended to "publish_dir" path + publish_files = Groovy map where key = "file_ext" and value = "directory" to publish results for that file extension + The value of "directory" is appended to the standard "publish_dir" path as defined above. + If publish_files = null (unspecified) - All files are published. + If publish_files = false - No files are published. + suffix = File name suffix for output files. +---------------------------------------------------------------------------------------- +*/ + +params { + modules { + 'fastqc' { + args = "--quiet" + } + 'multiqc' { + args = "" + } + } +} diff --git a/conf/test.config b/conf/test.config index 25379203..0d7c32bf 100644 --- a/conf/test.config +++ b/conf/test.config @@ -1,28 +1,29 @@ /* - * ------------------------------------------------- - * Nextflow config file for running tests - * ------------------------------------------------- - * Defines bundled input files and everything required - * to run a fast and simple test. Use as follows: - * nextflow run nf-core/bacass -profile test, - */ +======================================================================================== + Nextflow config file for running minimal tests +======================================================================================== + Defines input files and everything required to run a fast and simple pipeline test. + + Use as follows: + nextflow run nf-core/bacass -profile test, + +---------------------------------------------------------------------------------------- +*/ params { - config_profile_name = 'Test profile' - config_profile_description = 'Minimal test dataset to check pipeline function' - // Limit resources so that this can run on GitHub Actions - max_cpus = 2 - max_memory = 6.GB - max_time = 48.h + config_profile_name = 'Test profile' + config_profile_description = 'Minimal test dataset to check pipeline function' + + // Limit resources so that this can run on GitHub Actions + max_cpus = 2 + max_memory = 6.GB + max_time = 6.h + + // Input data + // TODO nf-core: Specify the paths to your test data on nf-core/test-datasets + // TODO nf-core: Give any required params for the test so that command line flags are not needed + input = 'https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv' - // Input data - // TODO nf-core: Specify the paths to your test data on nf-core/test-datasets - // TODO nf-core: Give any required params for the test so that command line flags are not needed - single_end = false - input_paths = [ - ['Testdata', ['https://github.com/nf-core/test-datasets/raw/exoseq/testdata/Testdata_R1.tiny.fastq.gz', 'https://github.com/nf-core/test-datasets/raw/exoseq/testdata/Testdata_R2.tiny.fastq.gz']], - ['SRR389222', ['https://github.com/nf-core/test-datasets/raw/methylseq/testdata/SRR389222_sub1.fastq.gz', 'https://github.com/nf-core/test-datasets/raw/methylseq/testdata/SRR389222_sub2.fastq.gz']] - ] - // Ignore `--input` as otherwise the parameter validation will throw an error - schema_ignore_params = 'genomes,input_paths,input' + // Genome references + genome = 'R64-1-1' } diff --git a/conf/test_full.config b/conf/test_full.config index 9c41bbdf..1bac8cd3 100644 --- a/conf/test_full.config +++ b/conf/test_full.config @@ -1,24 +1,24 @@ /* - * ------------------------------------------------- - * Nextflow config file for running full-size tests - * ------------------------------------------------- - * Defines bundled input files and everything required - * to run a full size pipeline test. Use as follows: - * nextflow run nf-core/bacass -profile test_full, - */ +======================================================================================== + Nextflow config file for running full-size tests +======================================================================================== + Defines input files and everything required to run a full size pipeline test. + + Use as follows: + nextflow run nf-core/bacass -profile test_full, + +---------------------------------------------------------------------------------------- +*/ params { - config_profile_name = 'Full test profile' - config_profile_description = 'Full test dataset to check pipeline function' + config_profile_name = 'Full test profile' + config_profile_description = 'Full test dataset to check pipeline function' + + // Input data for full size test + // TODO nf-core: Specify the paths to your full test data ( on nf-core/test-datasets or directly in repositories, e.g. SRA) + // TODO nf-core: Give any required params for the test so that command line flags are not needed + input = 'https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_full_illumina_amplicon.csv' - // Input data for full size test - // TODO nf-core: Specify the paths to your full test data ( on nf-core/test-datasets or directly in repositories, e.g. SRA) - // TODO nf-core: Give any required params for the test so that command line flags are not needed - single_end = false - input_paths = [ - ['Testdata', ['https://github.com/nf-core/test-datasets/raw/exoseq/testdata/Testdata_R1.tiny.fastq.gz', 'https://github.com/nf-core/test-datasets/raw/exoseq/testdata/Testdata_R2.tiny.fastq.gz']], - ['SRR389222', ['https://github.com/nf-core/test-datasets/raw/methylseq/testdata/SRR389222_sub1.fastq.gz', 'https://github.com/nf-core/test-datasets/raw/methylseq/testdata/SRR389222_sub2.fastq.gz']] - ] - // Ignore `--input` as otherwise the parameter validation will throw an error - schema_ignore_params = 'genomes,input_paths,input' + // Genome references + genome = 'R64-1-1' } diff --git a/docs/README.md b/docs/README.md index fc6b898b..5fbf9904 100644 --- a/docs/README.md +++ b/docs/README.md @@ -3,8 +3,8 @@ The nf-core/bacass documentation is split into the following pages: * [Usage](usage.md) - * An overview of how the pipeline works, how to run it and a description of all of the different command-line flags. + * An overview of how the pipeline works, how to run it and a description of all of the different command-line flags. * [Output](output.md) - * An overview of the different results produced by the pipeline and how to interpret them. + * An overview of the different results produced by the pipeline and how to interpret them. You can find a lot more documentation about installing, configuring and running nf-core pipelines on the website: [https://nf-co.re](https://nf-co.re) diff --git a/docs/images/mqc_fastqc_adapter.png b/docs/images/mqc_fastqc_adapter.png new file mode 100755 index 0000000000000000000000000000000000000000..361d0e47acfb424dea1f326590d1eb2f6dfa26b5 GIT binary patch literal 23458 zcmeFZ2UJtryD!S#x<#o93es(Ww4k)maRbte0-+a?-g^xY-3myTE`8G_KvA54)F1tn})nJ5u%TA4Y;^!^{48eL_}p#q-Umo0M|F1 z74+PQh^X8N|9_jcWbq~ zzn+tZC9B75nKdz=gQ8wo9GJ$P{D~3knlI_`-PRhCw34f1oYDLr^;oEbgxa#A^J%*2 z>FfDE*(~JzKFs$t_oeLz))qDU?s}%Q?7b~3Y;lUi^Oy-2@3g?joA4Wkgb6-2=ih*jub)~7yZ`T=L=Z`B`{1jhkB-iSjea94&Eo9A zxN59pv1p_}RO1>EC^q}Z2)ZI;b7JV_x4lMr=Bker2+EK;8~!;JO7re*@ZkDmoV878S*N^yX(F@U1yqt?Is3nnV>7}#(5pk`V3C) zWhB8;CwWIwsVIjH+`<9=YA(j&3DgQdFOOGU~*`36wNC&QDv8> zr?h2PQgnHkp&t^S)q^K!68h~`$PjZW&-Wns;Zlw$M2sc z1xR!u{m|Kih*|Hht#M@eOMM#8O*={^6b9k5B5^eBsrnhVHD7XZ5BWO&F?q(>Y=QFl z`f>yQ9NCoxZCH-1F{#mz_j{QeyY~4h*VeyYZ#S@Z(Pnb7G=ud!RW)5svqM*&GI_za 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the following steps: +The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes data using the following steps: -* [FastQC](#fastqc) - Read quality control -* [MultiQC](#multiqc) - Aggregate report describing results from the whole pipeline +* [FastQC](#fastqc) - Raw read QC +* [MultiQC](#multiqc) - Aggregate report describing results and QC from the whole pipeline * [Pipeline information](#pipeline-information) - Report metrics generated during the workflow execution -## FastQC +### FastQC -[FastQC](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/) gives general quality metrics about your sequenced reads. It provides information about the quality score distribution across your reads, per base sequence content (%A/T/G/C), adapter contamination and overrepresented sequences. +
+Output files -For further reading and documentation see the [FastQC help pages](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/Help/). +* `fastqc/` + * `*_fastqc.html`: FastQC report containing quality metrics. + * `*_fastqc.zip`: Zip archive containing the FastQC report, tab-delimited data file and plot images. -**Output files:** +
-* `fastqc/` - * `*_fastqc.html`: FastQC report containing quality metrics for your untrimmed raw fastq files. -* `fastqc/zips/` - * `*_fastqc.zip`: Zip archive containing the FastQC report, tab-delimited data file and plot images. +[FastQC](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/) gives general quality metrics about your sequenced reads. It provides information about the quality score distribution across your reads, per base sequence content (%A/T/G/C), adapter contamination and overrepresented sequences. For further reading and documentation see the [FastQC help pages](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/Help/). -> **NB:** The FastQC plots displayed in the MultiQC report shows _untrimmed_ reads. They may contain adapter sequence and potentially regions with low quality. +![MultiQC - FastQC sequence counts plot](images/mqc_fastqc_counts.png) -## MultiQC +![MultiQC - FastQC mean quality scores plot](images/mqc_fastqc_quality.png) -[MultiQC](http://multiqc.info) is a visualization tool that generates a single HTML report summarizing all samples in your project. Most of the pipeline QC results are visualised in the report and further statistics are available in the report data directory. +![MultiQC - FastQC adapter content plot](images/mqc_fastqc_adapter.png) -The pipeline has special steps which also allow the software versions to be reported in the MultiQC output for future traceability. +> **NB:** The FastQC plots displayed in the MultiQC report shows _untrimmed_ reads. They may contain adapter sequence and potentially regions with low quality. -For more information about how to use MultiQC reports, see [https://multiqc.info](https://multiqc.info). +### MultiQC -**Output files:** +
+Output files * `multiqc/` - * `multiqc_report.html`: a standalone HTML file that can be viewed in your web browser. - * `multiqc_data/`: directory containing parsed statistics from the different tools used in the pipeline. - * `multiqc_plots/`: directory containing static images from the report in various formats. + * `multiqc_report.html`: a standalone HTML file that can be viewed in your web browser. + * `multiqc_data/`: directory containing parsed statistics from the different tools used in the pipeline. + * `multiqc_plots/`: directory containing static images from the report in various formats. -## Pipeline information +
-[Nextflow](https://www.nextflow.io/docs/latest/tracing.html) provides excellent functionality for generating various reports relevant to the running and execution of the pipeline. This will allow you to troubleshoot errors with the running of the pipeline, and also provide you with other information such as launch commands, run times and resource usage. +[MultiQC](http://multiqc.info) is a visualization tool that generates a single HTML report summarising all samples in your project. Most of the pipeline QC results are visualised in the report and further statistics are available in the report data directory. -**Output files:** +Results generated by MultiQC collate pipeline QC from supported tools e.g. FastQC. The pipeline has special steps which also allow the software versions to be reported in the MultiQC output for future traceability. For more information about how to use MultiQC reports, see . + +### Pipeline information + +
+Output files * `pipeline_info/` - * Reports generated by Nextflow: `execution_report.html`, `execution_timeline.html`, `execution_trace.txt` and `pipeline_dag.dot`/`pipeline_dag.svg`. - * Reports generated by the pipeline: `pipeline_report.html`, `pipeline_report.txt` and `software_versions.csv`. - * Documentation for interpretation of results in HTML format: `results_description.html`. + * Reports generated by Nextflow: `execution_report.html`, `execution_timeline.html`, `execution_trace.txt` and `pipeline_dag.dot`/`pipeline_dag.svg`. + * Reports generated by the pipeline: `pipeline_report.html`, `pipeline_report.txt` and `software_versions.tsv`. + * Reformatted samplesheet files used as input to the pipeline: `samplesheet.valid.csv`. + +
+ +[Nextflow](https://www.nextflow.io/docs/latest/tracing.html) provides excellent functionality for generating various reports relevant to the running and execution of the pipeline. This will allow you to troubleshoot errors with the running of the pipeline, and also provide you with other information such as launch commands, run times and resource usage. diff --git a/docs/usage.md b/docs/usage.md index 38d77cb0..b50bca2f 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -8,19 +8,63 @@ +## Samplesheet input + +You will need to create a samplesheet with information about the samples you would like to analyse before running the pipeline. Use this parameter to specify its location. It has to be a comma-separated file with 3 columns, and a header row as shown in the examples below. + +```console +--input '[path to samplesheet file]' +``` + +### Multiple runs of the same sample + +The `sample` identifiers have to be the same when you have re-sequenced the same sample more than once e.g. to increase sequencing depth. The pipeline will concatenate the raw reads before performing any downstream analysis. Below is an example for the same sample sequenced across 3 lanes: + +```console +sample,fastq_1,fastq_2 +CONTROL_REP1,AEG588A1_S1_L002_R1_001.fastq.gz,AEG588A1_S1_L002_R2_001.fastq.gz +CONTROL_REP1,AEG588A1_S1_L003_R1_001.fastq.gz,AEG588A1_S1_L003_R2_001.fastq.gz +CONTROL_REP1,AEG588A1_S1_L004_R1_001.fastq.gz,AEG588A1_S1_L004_R2_001.fastq.gz +``` + +### Full samplesheet + +The pipeline will auto-detect whether a sample is single- or paired-end using the information provided in the samplesheet. The samplesheet can have as many columns as you desire, however, there is a strict requirement for the first 3 columns to match those defined in the table below. + +A final samplesheet file consisting of both single- and paired-end data may look something like the one below. This is for 6 samples, where `TREATMENT_REP3` has been sequenced twice. + +```console +sample,fastq_1,fastq_2 +CONTROL_REP1,AEG588A1_S1_L002_R1_001.fastq.gz,AEG588A1_S1_L002_R2_001.fastq.gz +CONTROL_REP2,AEG588A2_S2_L002_R1_001.fastq.gz,AEG588A2_S2_L002_R2_001.fastq.gz +CONTROL_REP3,AEG588A3_S3_L002_R1_001.fastq.gz,AEG588A3_S3_L002_R2_001.fastq.gz +TREATMENT_REP1,AEG588A4_S4_L003_R1_001.fastq.gz, +TREATMENT_REP2,AEG588A5_S5_L003_R1_001.fastq.gz, +TREATMENT_REP3,AEG588A6_S6_L003_R1_001.fastq.gz, +TREATMENT_REP3,AEG588A6_S6_L004_R1_001.fastq.gz, +``` + +| Column | Description | +|----------------|----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------| +| `sample` | Custom sample name. This entry will be identical for multiple sequencing libraries/runs from the same sample. Spaces in sample names are automatically converted to underscores (`_`). | +| `fastq_1` | Full path to FastQ file for Illumina short reads 1. File has to be gzipped and have the extension ".fastq.gz" or ".fq.gz". | +| `fastq_2` | Full path to FastQ file for Illumina short reads 2. File has to be gzipped and have the extension ".fastq.gz" or ".fq.gz". | + +An [example samplesheet](../assets/samplesheet.csv) has been provided with the pipeline. + ## Running the pipeline The typical command for running the pipeline is as follows: -```bash -nextflow run nf-core/bacass --input '*_R{1,2}.fastq.gz' -profile docker +```console +nextflow run nf-core/bacass --input samplesheet.csv --genome GRCh37 -profile docker ``` This will launch the pipeline with the `docker` configuration profile. See below for more information about profiles. Note that the pipeline will create the following files in your working directory: -```bash +```console work # Directory containing the nextflow working files results # Finished results (configurable, see below) .nextflow_log # Log file from Nextflow @@ -31,13 +75,13 @@ results # Finished results (configurable, see below) When you run the above command, Nextflow automatically pulls the pipeline code from GitHub and stores it as a cached version. When running the pipeline after this, it will always use the cached version if available - even if the pipeline has been updated since. To make sure that you're running the latest version of the pipeline, make sure that you regularly update the cached version of the pipeline: -```bash +```console nextflow pull nf-core/bacass ``` ### Reproducibility -It's a good idea to specify a pipeline version when running the pipeline on your data. This ensures that a specific version of the pipeline code and software are used when you run your pipeline. If you keep using the same tag, you'll be running the same version of the pipeline, even if there have been changes to the code since. +It is a good idea to specify a pipeline version when running the pipeline on your data. This ensures that a specific version of the pipeline code and software are used when you run your pipeline. If you keep using the same tag, you'll be running the same version of the pipeline, even if there have been changes to the code since. First, go to the [nf-core/bacass releases page](https://github.com/nf-core/bacass/releases) and find the latest version number - numeric only (eg. `1.3.1`). Then specify this when running the pipeline with `-r` (one hyphen) - eg. `-r 1.3.1`. @@ -51,7 +95,7 @@ This version number will be logged in reports when you run the pipeline, so that Use this parameter to choose a configuration profile. Profiles can give configuration presets for different compute environments. -Several generic profiles are bundled with the pipeline which instruct the pipeline to use software packaged using different methods (Docker, Singularity, Podman, Shifter, Charliecloud, Conda) - see below. +Several generic profiles are bundled with the pipeline which instruct the pipeline to use software packaged using different methods (Docker, Singularity, Podman, Shifter, Charliecloud, Conda) - see below. When using Biocontainers, most of these software packaging methods pull Docker containers from quay.io e.g [FastQC](https://quay.io/repository/biocontainers/fastqc) except for Singularity which directly downloads Singularity images via https hosted by the [Galaxy project](https://depot.galaxyproject.org/singularity/) and Conda which downloads and installs software locally from [Bioconda](https://bioconda.github.io/). > We highly recommend the use of Docker or Singularity containers for full pipeline reproducibility, however when this is not possible, Conda is also supported. @@ -63,27 +107,20 @@ They are loaded in sequence, so later profiles can overwrite earlier profiles. If `-profile` is not specified, the pipeline will run locally and expect all software to be installed and available on the `PATH`. This is _not_ recommended. * `docker` - * A generic configuration profile to be used with [Docker](https://docker.com/) - * Pulls software from Docker Hub: [`nfcore/bacass`](https://hub.docker.com/r/nfcore/bacass/) + * A generic configuration profile to be used with [Docker](https://docker.com/) * `singularity` - * A generic configuration profile to be used with [Singularity](https://sylabs.io/docs/) - * Pulls software from Docker Hub: [`nfcore/bacass`](https://hub.docker.com/r/nfcore/bacass/) + * A generic configuration profile to be used with [Singularity](https://sylabs.io/docs/) * `podman` - * A generic configuration profile to be used with [Podman](https://podman.io/) - * Pulls software from Docker Hub: [`nfcore/bacass`](https://hub.docker.com/r/nfcore/bacass/) + * A generic configuration profile to be used with [Podman](https://podman.io/) * `shifter` - * A generic configuration profile to be used with [Shifter](https://nersc.gitlab.io/development/shifter/how-to-use/) - * Pulls software from Docker Hub: [`nfcore/bacass`](https://hub.docker.com/r/nfcore/bacass/) + * A generic configuration profile to be used with [Shifter](https://nersc.gitlab.io/development/shifter/how-to-use/) * `charliecloud` - * A generic configuration profile to be used with [Charliecloud](https://hpc.github.io/charliecloud/) - * Pulls software from Docker Hub: [`nfcore/bacass`](https://hub.docker.com/r/nfcore/bacass/) + * A generic configuration profile to be used with [Charliecloud](https://hpc.github.io/charliecloud/) * `conda` - * Please only use Conda as a last resort i.e. when it's not possible to run the pipeline with Docker, Singularity, Podman, Shifter or Charliecloud. - * A generic configuration profile to be used with [Conda](https://conda.io/docs/) - * Pulls most software from [Bioconda](https://bioconda.github.io/) + * A generic configuration profile to be used with [Conda](https://conda.io/docs/). Please only use Conda as a last resort i.e. when it's not possible to run the pipeline with Docker, Singularity, Podman, Shifter or Charliecloud. * `test` - * A profile with a complete configuration for automated testing - * Includes links to test data so needs no other parameters + * A profile with a complete configuration for automated testing + * Includes links to test data so needs no other parameters ### `-resume` @@ -95,29 +132,140 @@ You can also supply a run name to resume a specific run: `-resume [run-name]`. U Specify the path to a specific config file (this is a core Nextflow command). See the [nf-core website documentation](https://nf-co.re/usage/configuration) for more information. -#### Custom resource requests +## Custom configuration + +### Resource requests + +Whilst the default requirements set within the pipeline will hopefully work for most people and with most input data, you may find that you want to customise the compute resources that the pipeline requests. Each step in the pipeline has a default set of requirements for number of CPUs, memory and time. For most of the steps in the pipeline, if the job exits with any of the error codes specified [here](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/conf/base.config#L18) it will automatically be resubmitted with higher requests (2 x original, then 3 x original). If it still fails after the third attempt then the pipeline execution is stopped. + +For example, if the nf-core/rnaseq pipeline is failing after multiple re-submissions of the `STAR_ALIGN` process due to an exit code of `137` this would indicate that there is an out of memory issue: + +```console +[62/149eb0] NOTE: Process `RNASEQ:ALIGN_STAR:STAR_ALIGN (WT_REP1)` terminated with an error exit status (137) -- Execution is retried (1) +Error executing process > 'RNASEQ:ALIGN_STAR:STAR_ALIGN (WT_REP1)' + +Caused by: + Process `RNASEQ:ALIGN_STAR:STAR_ALIGN (WT_REP1)` terminated with an error exit status (137) -Each step in the pipeline has a default set of requirements for number of CPUs, memory and time. For most of the steps in the pipeline, if the job exits with an error code of `143` (exceeded requested resources) it will automatically resubmit with higher requests (2 x original, then 3 x original). If it still fails after three times then the pipeline is stopped. +Command executed: + STAR \ + --genomeDir star \ + --readFilesIn WT_REP1_trimmed.fq.gz \ + --runThreadN 2 \ + --outFileNamePrefix WT_REP1. \ + -Whilst these default requirements will hopefully work for most people with most data, you may find that you want to customise the compute resources that the pipeline requests. You can do this by creating a custom config file. For example, to give the workflow process `star` 32GB of memory, you could use the following config: +Command exit status: + 137 + +Command output: + (empty) + +Command error: + .command.sh: line 9: 30 Killed STAR --genomeDir star --readFilesIn WT_REP1_trimmed.fq.gz --runThreadN 2 --outFileNamePrefix WT_REP1. +Work dir: + /home/pipelinetest/work/9d/172ca5881234073e8d76f2a19c88fb + +Tip: you can replicate the issue by changing to the process work dir and entering the command `bash .command.run` +``` + +To bypass this error you would need to find exactly which resources are set by the `STAR_ALIGN` process. The quickest way is to search for `process STAR_ALIGN` in the [nf-core/rnaseq Github repo](https://github.com/nf-core/rnaseq/search?q=process+STAR_ALIGN). We have standardised the structure of Nextflow DSL2 pipelines such that all module files will be present in the `modules/` directory and so based on the search results the file we want is `modules/nf-core/software/star/align/main.nf`. If you click on the link to that file you will notice that there is a `label` directive at the top of the module that is set to [`label process_high`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/modules/nf-core/software/star/align/main.nf#L9). The [Nextflow `label`](https://www.nextflow.io/docs/latest/process.html#label) directive allows us to organise workflow processes in separate groups which can be referenced in a configuration file to select and configure subset of processes having similar computing requirements. The default values for the `process_high` label are set in the pipeline's [`base.config`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/conf/base.config#L33-L37) which in this case is defined as 72GB. Providing you haven't set any other standard nf-core parameters to __cap__ the [maximum resources](https://nf-co.re/usage/configuration#max-resources) used by the pipeline then we can try and bypass the `STAR_ALIGN` process failure by creating a custom config file that sets at least 72GB of memory, in this case increased to 100GB. The custom config below can then be provided to the pipeline via the [`-c`](#-c) parameter as highlighted in previous sections. ```nextflow process { - withName: star { - memory = 32.GB - } + withName: STAR_ALIGN { + memory = 100.GB + } } ``` -To find the exact name of a process you wish to modify the compute resources, check the live-status of a nextflow run displayed on your terminal or check the nextflow error for a line like so: `Error executing process > 'bwa'`. In this case the name to specify in the custom config file is `bwa`. +> **NB:** We specify just the process name i.e. `STAR_ALIGN` in the config file and not the full task name string that is printed to screen in the error message or on the terminal whilst the pipeline is running i.e. `RNASEQ:ALIGN_STAR:STAR_ALIGN`. You may get a warning suggesting that the process selector isn't recognised but you can ignore that if the process name has been specified correctly. This is something that needs to be fixed upstream in core Nextflow. + +### Tool-specific options + +For the ultimate flexibility, we have implemented and are using Nextflow DSL2 modules in a way where it is possible for both developers and users to change tool-specific command-line arguments (e.g. providing an additional command-line argument to the `STAR_ALIGN` process) as well as publishing options (e.g. saving files produced by the `STAR_ALIGN` process that aren't saved by default by the pipeline). In the majority of instances, as a user you won't have to change the default options set by the pipeline developer(s), however, there may be edge cases where creating a simple custom config file can improve the behaviour of the pipeline if for example it is failing due to a weird error that requires setting a tool-specific parameter to deal with smaller / larger genomes. + +The command-line arguments passed to STAR in the `STAR_ALIGN` module are a combination of: + +* Mandatory arguments or those that need to be evaluated within the scope of the module, as supplied in the [`script`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/modules/nf-core/software/star/align/main.nf#L49-L55) section of the module file. + +* An [`options.args`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/modules/nf-core/software/star/align/main.nf#L56) string of non-mandatory parameters that is set to be empty by default in the module but can be overwritten when including the module in the sub-workflow / workflow context via the `addParams` Nextflow option. + +The nf-core/rnaseq pipeline has a sub-workflow (see [terminology](https://github.com/nf-core/modules#terminology)) specifically to align reads with STAR and to sort, index and generate some basic stats on the resulting BAM files using SAMtools. At the top of this file we import the `STAR_ALIGN` module via the Nextflow [`include`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/subworkflows/nf-core/align_star.nf#L10) keyword and by default the options passed to the module via the `addParams` option are set as an empty Groovy map [here](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/subworkflows/nf-core/align_star.nf#L5); this in turn means `options.args` will be set to empty by default in the module file too. This is an intentional design choice and allows us to implement well-written sub-workflows composed of a chain of tools that by default run with the bare minimum parameter set for any given tool in order to make it much easier to share across pipelines and to provide the flexibility for users and developers to customise any non-mandatory arguments. + +When including the sub-workflow above in the main pipeline workflow we use the same `include` statement, however, we now have the ability to overwrite options for each of the tools in the sub-workflow including the [`align_options`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/workflows/rnaseq.nf#L225) variable that will be used specifically to overwrite the optional arguments passed to the `STAR_ALIGN` module. In this case, the options to be provided to `STAR_ALIGN` have been assigned sensible defaults by the developer(s) in the pipeline's [`modules.config`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/conf/modules.config#L70-L74) and can be accessed and customised in the [workflow context](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/workflows/rnaseq.nf#L201-L204) too before eventually passing them to the sub-workflow as a Groovy map called `star_align_options`. These options will then be propagated from `workflow -> sub-workflow -> module`. + +As mentioned at the beginning of this section it may also be necessary for users to overwrite the options passed to modules to be able to customise specific aspects of the way in which a particular tool is executed by the pipeline. Given that all of the default module options are stored in the pipeline's `modules.config` as a [`params` variable](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/conf/modules.config#L24-L25) it is also possible to overwrite any of these options via a custom config file. + +Say for example we want to append an additional, non-mandatory parameter (i.e. `--outFilterMismatchNmax 16`) to the arguments passed to the `STAR_ALIGN` module. Firstly, we need to copy across the default `args` specified in the [`modules.config`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/conf/modules.config#L71) and create a custom config file that is a composite of the default `args` as well as the additional options you would like to provide. This is very important because Nextflow will overwrite the default value of `args` that you provide via the custom config. + +As you will see in the example below, we have: + +* appended `--outFilterMismatchNmax 16` to the default `args` used by the module. +* changed the default `publish_dir` value to where the files will eventually be published in the main results directory. +* appended `'bam':''` to the default value of `publish_files` so that the BAM files generated by the process will also be saved in the top-level results directory for the module. Note: `'out':'log'` means any file/directory ending in `out` will now be saved in a separate directory called `my_star_directory/log/`. + +```nextflow +params { + modules { + 'star_align' { + args = "--quantMode TranscriptomeSAM --twopassMode Basic --outSAMtype BAM Unsorted --readFilesCommand zcat --runRNGseed 0 --outFilterMultimapNmax 20 --alignSJDBoverhangMin 1 --outSAMattributes NH HI AS NM MD --quantTranscriptomeBan Singleend --outFilterMismatchNmax 16" + publish_dir = "my_star_directory" + publish_files = ['out':'log', 'tab':'log', 'bam':''] + } + } +} +``` + +### Updating containers + +The [Nextflow DSL2](https://www.nextflow.io/docs/latest/dsl2.html) implementation of this pipeline uses one container per process which makes it much easier to maintain and update software dependencies. If for some reason you need to use a different version of a particular tool with the pipeline then you just need to identify the `process` name and override the Nextflow `container` definition for that process using the `withName` declaration. For example, in the [nf-core/viralrecon](https://nf-co.re/viralrecon) pipeline a tool called [Pangolin](https://github.com/cov-lineages/pangolin) has been used during the COVID-19 pandemic to assign lineages to SARS-CoV-2 genome sequenced samples. Given that the lineage assignments change quite frequently it doesn't make sense to re-release the nf-core/viralrecon everytime a new version of Pangolin has been released. However, you can override the default container used by the pipeline by creating a custom config file and passing it as a command-line argument via `-c custom.config`. + +1. Check the default version used by the pipeline in the module file for [Pangolin](https://github.com/nf-core/viralrecon/blob/a85d5969f9025409e3618d6c280ef15ce417df65/modules/nf-core/software/pangolin/main.nf#L14-L19) +2. Find the latest version of the Biocontainer available on [Quay.io](https://quay.io/repository/biocontainers/pangolin?tag=latest&tab=tags) +3. Create the custom config accordingly: + + * For Docker: + + ```nextflow + process { + withName: PANGOLIN { + container = 'quay.io/biocontainers/pangolin:3.0.5--pyhdfd78af_0' + } + } + ``` + + * For Singularity: + + ```nextflow + process { + withName: PANGOLIN { + container = 'https://depot.galaxyproject.org/singularity/pangolin:3.0.5--pyhdfd78af_0' + } + } + ``` + + * For Conda: + + ```nextflow + process { + withName: PANGOLIN { + conda = 'bioconda::pangolin=3.0.5' + } + } + ``` + +> **NB:** If you wish to periodically update individual tool-specific results (e.g. Pangolin) generated by the pipeline then you must ensure to keep the `work/` directory otherwise the `-resume` ability of the pipeline will be compromised and it will restart from scratch. + +### nf-core/configs -See the main [Nextflow documentation](https://www.nextflow.io/docs/latest/config.html) for more information. +In most cases, you will only need to create a custom config as a one-off but if you and others within your organisation are likely to be running nf-core pipelines regularly and need to use the same settings regularly it may be a good idea to request that your custom config file is uploaded to the `nf-core/configs` git repository. Before you do this please can you test that the config file works with your pipeline of choice using the `-c` parameter. You can then create a pull request to the `nf-core/configs` repository with the addition of your config file, associated documentation file (see examples in [`nf-core/configs/docs`](https://github.com/nf-core/configs/tree/master/docs)), and amending [`nfcore_custom.config`](https://github.com/nf-core/configs/blob/master/nfcore_custom.config) to include your custom profile. -If you are likely to be running `nf-core` pipelines regularly it may be a good idea to request that your custom config file is uploaded to the `nf-core/configs` git repository. Before you do this please can you test that the config file works with your pipeline of choice using the `-c` parameter (see definition above). You can then create a pull request to the `nf-core/configs` repository with the addition of your config file, associated documentation file (see examples in [`nf-core/configs/docs`](https://github.com/nf-core/configs/tree/master/docs)), and amending [`nfcore_custom.config`](https://github.com/nf-core/configs/blob/master/nfcore_custom.config) to include your custom profile. +See the main [Nextflow documentation](https://www.nextflow.io/docs/latest/config.html) for more information about creating your own configuration files. If you have any questions or issues please send us a message on [Slack](https://nf-co.re/join/slack) on the [`#configs` channel](https://nfcore.slack.com/channels/configs). -### Running in the background +## Running in the background Nextflow handles job submissions and supervises the running jobs. The Nextflow process must run until the pipeline is finished. @@ -126,11 +274,11 @@ The Nextflow `-bg` flag launches Nextflow in the background, detached from your Alternatively, you can use `screen` / `tmux` or similar tool to create a detached session which you can log back into at a later time. Some HPC setups also allow you to run nextflow within a cluster job submitted your job scheduler (from where it submits more jobs). -#### Nextflow memory requirements +## Nextflow memory requirements In some cases, the Nextflow Java virtual machines can start to request a large amount of memory. We recommend adding the following line to your environment to limit this (typically in `~/.bashrc` or `~./bash_profile`): -```bash +```console NXF_OPTS='-Xms1g -Xmx4g' ``` diff --git a/environment.yml b/environment.yml deleted file mode 100644 index 0e316597..00000000 --- a/environment.yml +++ /dev/null @@ -1,15 +0,0 @@ -# You can use this file to create a conda environment for this pipeline: -# conda env create -f environment.yml -name: nf-core-bacass-1.1.1 -channels: - - conda-forge - - bioconda - - defaults -dependencies: - - conda-forge::python=3.7.3 - - conda-forge::markdown=3.1.1 - - conda-forge::pymdown-extensions=6.0 - - conda-forge::pygments=2.5.2 - # TODO nf-core: Add required software dependencies here - - bioconda::fastqc=0.11.8 - - bioconda::multiqc=1.7 diff --git a/lib/Headers.groovy b/lib/Headers.groovy deleted file mode 100644 index 15d1d388..00000000 --- a/lib/Headers.groovy +++ /dev/null @@ -1,43 +0,0 @@ -/* - * This file holds several functions used to render the nf-core ANSI header. - */ - -class Headers { - - private static Map log_colours(Boolean monochrome_logs) { - Map colorcodes = [:] - colorcodes['reset'] = monochrome_logs ? '' : "\033[0m" - colorcodes['dim'] = monochrome_logs ? '' : "\033[2m" - colorcodes['black'] = monochrome_logs ? '' : "\033[0;30m" - colorcodes['green'] = monochrome_logs ? '' : "\033[0;32m" - colorcodes['yellow'] = monochrome_logs ? '' : "\033[0;33m" - colorcodes['yellow_bold'] = monochrome_logs ? '' : "\033[1;93m" - colorcodes['blue'] = monochrome_logs ? '' : "\033[0;34m" - colorcodes['purple'] = monochrome_logs ? '' : "\033[0;35m" - colorcodes['cyan'] = monochrome_logs ? '' : "\033[0;36m" - colorcodes['white'] = monochrome_logs ? '' : "\033[0;37m" - colorcodes['red'] = monochrome_logs ? '' : "\033[1;91m" - return colorcodes - } - - static String dashed_line(monochrome_logs) { - Map colors = log_colours(monochrome_logs) - return "-${colors.dim}----------------------------------------------------${colors.reset}-" - } - - static String nf_core(workflow, monochrome_logs) { - Map colors = log_colours(monochrome_logs) - String.format( - """\n - ${dashed_line(monochrome_logs)} - ${colors.green},--.${colors.black}/${colors.green},-.${colors.reset} - ${colors.blue} ___ __ __ __ ___ ${colors.green}/,-._.--~\'${colors.reset} - ${colors.blue} |\\ | |__ __ / ` / \\ |__) |__ ${colors.yellow}} {${colors.reset} - ${colors.blue} | \\| | \\__, \\__/ | \\ |___ ${colors.green}\\`-._,-`-,${colors.reset} - ${colors.green}`._,._,\'${colors.reset} - ${colors.purple} ${workflow.manifest.name} v${workflow.manifest.version}${colors.reset} - ${dashed_line(monochrome_logs)} - """.stripIndent() - ) - } -} diff --git a/lib/NfcoreSchema.groovy b/lib/NfcoreSchema.groovy old mode 100644 new mode 100755 index 52ee7304..8d6920dd --- a/lib/NfcoreSchema.groovy +++ b/lib/NfcoreSchema.groovy @@ -1,6 +1,6 @@ -/* - * This file holds several functions used to perform JSON parameter validation, help and summary rendering for the nf-core pipeline template. - */ +// +// This file holds several functions used to perform JSON parameter validation, help and summary rendering for the nf-core pipeline template. +// import org.everit.json.schema.Schema import org.everit.json.schema.loader.SchemaLoader @@ -13,16 +13,23 @@ import groovy.json.JsonBuilder class NfcoreSchema { - /* - * Function to loop over all parameters defined in schema and check - * whether the given paremeters adhere to the specificiations - */ + // + // Resolve Schema path relative to main workflow directory + // + public static String getSchemaPath(workflow, schema_filename='nextflow_schema.json') { + return "${workflow.projectDir}/${schema_filename}" + } + + // + // Function to loop over all parameters defined in schema and check + // whether the given parameters adhere to the specifications + // /* groovylint-disable-next-line UnusedPrivateMethodParameter */ - private static void validateParameters(params, jsonSchema, log) { + public static void validateParameters(workflow, params, log, schema_filename='nextflow_schema.json') { def has_error = false //=====================================================================// // Check for nextflow core params and unexpected params - def json = new File(jsonSchema).text + def json = new File(getSchemaPath(workflow, schema_filename=schema_filename)).text def Map schemaParams = (Map) new JsonSlurper().parseText(json).get('definitions') def nf_params = [ // Options for base `nextflow` command @@ -114,7 +121,8 @@ class NfcoreSchema { def params_ignore = params.schema_ignore_params.split(',') + 'schema_ignore_params' def expectedParamsLowerCase = expectedParams.collect{ it.replace("-", "").toLowerCase() } def specifiedParamLowerCase = specifiedParam.replace("-", "").toLowerCase() - if (!expectedParams.contains(specifiedParam) && !params_ignore.contains(specifiedParam) && !expectedParamsLowerCase.contains(specifiedParamLowerCase)) { + def isCamelCaseBug = (specifiedParam.contains("-") && !expectedParams.contains(specifiedParam) && expectedParamsLowerCase.contains(specifiedParamLowerCase)) + if (!expectedParams.contains(specifiedParam) && !params_ignore.contains(specifiedParam) && !isCamelCaseBug) { // Temporarily remove camelCase/camel-case params #1035 def unexpectedParamsLowerCase = unexpectedParams.collect{ it.replace("-", "").toLowerCase()} if (!unexpectedParamsLowerCase.contains(specifiedParamLowerCase)){ @@ -125,36 +133,36 @@ class NfcoreSchema { //=====================================================================// // Validate parameters against the schema - InputStream inputStream = new File(jsonSchema).newInputStream() - JSONObject rawSchema = new JSONObject(new JSONTokener(inputStream)) + InputStream input_stream = new File(getSchemaPath(workflow, schema_filename=schema_filename)).newInputStream() + JSONObject raw_schema = new JSONObject(new JSONTokener(input_stream)) // Remove anything that's in params.schema_ignore_params - rawSchema = removeIgnoredParams(rawSchema, params) + raw_schema = removeIgnoredParams(raw_schema, params) - Schema schema = SchemaLoader.load(rawSchema) + Schema schema = SchemaLoader.load(raw_schema) // Clean the parameters def cleanedParams = cleanParameters(params) // Convert to JSONObject def jsonParams = new JsonBuilder(cleanedParams) - JSONObject paramsJSON = new JSONObject(jsonParams.toString()) + JSONObject params_json = new JSONObject(jsonParams.toString()) // Validate try { - schema.validate(paramsJSON) + schema.validate(params_json) } catch (ValidationException e) { println '' log.error 'ERROR: Validation of pipeline parameters failed!' JSONObject exceptionJSON = e.toJSON() - printExceptions(exceptionJSON, paramsJSON, log) + printExceptions(exceptionJSON, params_json, log) println '' has_error = true } // Check for unexpected parameters if (unexpectedParams.size() > 0) { - Map colors = log_colours(params.monochrome_logs) + Map colors = NfcoreTemplate.logColours(params.monochrome_logs) println '' def warn_msg = 'Found unexpected parameters:' for (unexpectedParam in unexpectedParams) { @@ -170,266 +178,17 @@ class NfcoreSchema { } } - // Loop over nested exceptions and print the causingException - private static void printExceptions(exJSON, paramsJSON, log) { - def causingExceptions = exJSON['causingExceptions'] - if (causingExceptions.length() == 0) { - def m = exJSON['message'] =~ /required key \[([^\]]+)\] not found/ - // Missing required param - if (m.matches()) { - log.error "* Missing required parameter: --${m[0][1]}" - } - // Other base-level error - else if (exJSON['pointerToViolation'] == '#') { - log.error "* ${exJSON['message']}" - } - // Error with specific param - else { - def param = exJSON['pointerToViolation'] - ~/^#\// - def param_val = paramsJSON[param].toString() - log.error "* --${param}: ${exJSON['message']} (${param_val})" - } - } - for (ex in causingExceptions) { - printExceptions(ex, paramsJSON, log) - } - } - - // Remove an element from a JSONArray - private static JSONArray removeElement(jsonArray, element){ - def list = [] - int len = jsonArray.length() - for (int i=0;i - if(rawSchema.keySet().contains('definitions')){ - rawSchema.definitions.each { definition -> - for (key in definition.keySet()){ - if (definition[key].get("properties").keySet().contains(ignore_param)){ - // Remove the param to ignore - definition[key].get("properties").remove(ignore_param) - // If the param was required, change this - if (definition[key].has("required")) { - def cleaned_required = removeElement(definition[key].required, ignore_param) - definition[key].put("required", cleaned_required) - } - } - } - } - } - if(rawSchema.keySet().contains('properties') && rawSchema.get('properties').keySet().contains(ignore_param)) { - rawSchema.get("properties").remove(ignore_param) - } - if(rawSchema.keySet().contains('required') && rawSchema.required.contains(ignore_param)) { - def cleaned_required = removeElement(rawSchema.required, ignore_param) - rawSchema.put("required", cleaned_required) - } - } - return rawSchema - } - - private static Map cleanParameters(params) { - def new_params = params.getClass().newInstance(params) - for (p in params) { - // remove anything evaluating to false - if (!p['value']) { - new_params.remove(p.key) - } - // Cast MemoryUnit to String - if (p['value'].getClass() == nextflow.util.MemoryUnit) { - new_params.replace(p.key, p['value'].toString()) - } - // Cast Duration to String - if (p['value'].getClass() == nextflow.util.Duration) { - new_params.replace(p.key, p['value'].toString().replaceFirst(/d(?!\S)/, "day")) - } - // Cast LinkedHashMap to String - if (p['value'].getClass() == LinkedHashMap) { - new_params.replace(p.key, p['value'].toString()) - } - } - return new_params - } - - /* - * This method tries to read a JSON params file - */ - private static LinkedHashMap params_load(String json_schema) { - def params_map = new LinkedHashMap() - try { - params_map = params_read(json_schema) - } catch (Exception e) { - println "Could not read parameters settings from JSON. $e" - params_map = new LinkedHashMap() - } - return params_map - } - - private static Map log_colours(Boolean monochrome_logs) { - Map colorcodes = [:] - - // Reset / Meta - colorcodes['reset'] = monochrome_logs ? '' : "\033[0m" - colorcodes['bold'] = monochrome_logs ? '' : "\033[1m" - colorcodes['dim'] = monochrome_logs ? '' : "\033[2m" - colorcodes['underlined'] = monochrome_logs ? '' : "\033[4m" - colorcodes['blink'] = monochrome_logs ? '' : "\033[5m" - colorcodes['reverse'] = monochrome_logs ? '' : "\033[7m" - colorcodes['hidden'] = monochrome_logs ? '' : "\033[8m" - - // Regular Colors - colorcodes['black'] = monochrome_logs ? '' : "\033[0;30m" - colorcodes['red'] = monochrome_logs ? '' : "\033[0;31m" - colorcodes['green'] = monochrome_logs ? '' : "\033[0;32m" - colorcodes['yellow'] = monochrome_logs ? '' : "\033[0;33m" - colorcodes['blue'] = monochrome_logs ? '' : "\033[0;34m" - colorcodes['purple'] = monochrome_logs ? '' : "\033[0;35m" - colorcodes['cyan'] = monochrome_logs ? '' : "\033[0;36m" - colorcodes['white'] = monochrome_logs ? '' : "\033[0;37m" - - // Bold - colorcodes['bblack'] = monochrome_logs ? '' : "\033[1;30m" - colorcodes['bred'] = monochrome_logs ? '' : "\033[1;31m" - colorcodes['bgreen'] = monochrome_logs ? '' : "\033[1;32m" - colorcodes['byellow'] = monochrome_logs ? '' : "\033[1;33m" - colorcodes['bblue'] = monochrome_logs ? '' : "\033[1;34m" - colorcodes['bpurple'] = monochrome_logs ? '' : "\033[1;35m" - colorcodes['bcyan'] = monochrome_logs ? '' : "\033[1;36m" - colorcodes['bwhite'] = monochrome_logs ? '' : "\033[1;37m" - - // Underline - colorcodes['ublack'] = monochrome_logs ? '' : "\033[4;30m" - colorcodes['ured'] = monochrome_logs ? '' : "\033[4;31m" - colorcodes['ugreen'] = monochrome_logs ? '' : "\033[4;32m" - colorcodes['uyellow'] = monochrome_logs ? '' : "\033[4;33m" - colorcodes['ublue'] = monochrome_logs ? '' : "\033[4;34m" - colorcodes['upurple'] = monochrome_logs ? '' : "\033[4;35m" - colorcodes['ucyan'] = monochrome_logs ? '' : "\033[4;36m" - colorcodes['uwhite'] = monochrome_logs ? '' : "\033[4;37m" - - // High Intensity - colorcodes['iblack'] = monochrome_logs ? '' : "\033[0;90m" - colorcodes['ired'] = monochrome_logs ? '' : "\033[0;91m" - colorcodes['igreen'] = monochrome_logs ? '' : "\033[0;92m" - colorcodes['iyellow'] = monochrome_logs ? '' : "\033[0;93m" - colorcodes['iblue'] = monochrome_logs ? '' : "\033[0;94m" - colorcodes['ipurple'] = monochrome_logs ? '' : "\033[0;95m" - colorcodes['icyan'] = monochrome_logs ? '' : "\033[0;96m" - colorcodes['iwhite'] = monochrome_logs ? '' : "\033[0;97m" - - // Bold High Intensity - colorcodes['biblack'] = monochrome_logs ? '' : "\033[1;90m" - colorcodes['bired'] = monochrome_logs ? '' : "\033[1;91m" - colorcodes['bigreen'] = monochrome_logs ? '' : "\033[1;92m" - colorcodes['biyellow'] = monochrome_logs ? '' : "\033[1;93m" - colorcodes['biblue'] = monochrome_logs ? '' : "\033[1;94m" - colorcodes['bipurple'] = monochrome_logs ? '' : "\033[1;95m" - colorcodes['bicyan'] = monochrome_logs ? '' : "\033[1;96m" - colorcodes['biwhite'] = monochrome_logs ? '' : "\033[1;97m" - - return colorcodes - } - - static String dashed_line(monochrome_logs) { - Map colors = log_colours(monochrome_logs) - return "-${colors.dim}----------------------------------------------------${colors.reset}-" - } - - /* - Method to actually read in JSON file using Groovy. - Group (as Key), values are all parameters - - Parameter1 as Key, Description as Value - - Parameter2 as Key, Description as Value - .... - Group - - - */ - private static LinkedHashMap params_read(String json_schema) throws Exception { - def json = new File(json_schema).text - def Map schema_definitions = (Map) new JsonSlurper().parseText(json).get('definitions') - def Map schema_properties = (Map) new JsonSlurper().parseText(json).get('properties') - /* Tree looks like this in nf-core schema - * definitions <- this is what the first get('definitions') gets us - group 1 - title - description - properties - parameter 1 - type - description - parameter 2 - type - description - group 2 - title - description - properties - parameter 1 - type - description - * properties <- parameters can also be ungrouped, outside of definitions - parameter 1 - type - description - */ - - // Grouped params - def params_map = new LinkedHashMap() - schema_definitions.each { key, val -> - def Map group = schema_definitions."$key".properties // Gets the property object of the group - def title = schema_definitions."$key".title - def sub_params = new LinkedHashMap() - group.each { innerkey, value -> - sub_params.put(innerkey, value) - } - params_map.put(title, sub_params) - } - - // Ungrouped params - def ungrouped_params = new LinkedHashMap() - schema_properties.each { innerkey, value -> - ungrouped_params.put(innerkey, value) - } - params_map.put("Other parameters", ungrouped_params) - - return params_map - } - - /* - * Get maximum number of characters across all parameter names - */ - private static Integer params_max_chars(params_map) { - Integer max_chars = 0 - for (group in params_map.keySet()) { - def group_params = params_map.get(group) // This gets the parameters of that particular group - for (param in group_params.keySet()) { - if (param.size() > max_chars) { - max_chars = param.size() - } - } - } - return max_chars - } - - /* - * Beautify parameters for --help - */ - private static String params_help(workflow, params, json_schema, command) { - Map colors = log_colours(params.monochrome_logs) + // + // Beautify parameters for --help + // + public static String paramsHelp(workflow, params, command, schema_filename='nextflow_schema.json') { + Map colors = NfcoreTemplate.logColours(params.monochrome_logs) Integer num_hidden = 0 String output = '' output += 'Typical pipeline command:\n\n' output += " ${colors.cyan}${command}${colors.reset}\n\n" - Map params_map = params_load(json_schema) - Integer max_chars = params_max_chars(params_map) + 1 + Map params_map = paramsLoad(getSchemaPath(workflow, schema_filename=schema_filename)) + Integer max_chars = paramsMaxChars(params_map) + 1 Integer desc_indent = max_chars + 14 Integer dec_linewidth = 160 - desc_indent for (group in params_map.keySet()) { @@ -469,18 +228,17 @@ class NfcoreSchema { output += group_output } } - output += dashed_line(params.monochrome_logs) if (num_hidden > 0){ - output += colors.dim + "\n Hiding $num_hidden params, use --show_hidden_params to show.\n" + colors.reset - output += dashed_line(params.monochrome_logs) + output += colors.dim + "!! Hiding $num_hidden params, use --show_hidden_params to show them !!\n" + colors.reset } + output += NfcoreTemplate.dashedLine(params.monochrome_logs) return output } - /* - * Groovy Map summarising parameters/workflow options used by the pipeline - */ - private static LinkedHashMap params_summary_map(workflow, params, json_schema) { + // + // Groovy Map summarising parameters/workflow options used by the pipeline + // + public static LinkedHashMap paramsSummaryMap(workflow, params, schema_filename='nextflow_schema.json') { // Get a selection of core Nextflow workflow options def Map workflow_summary = [:] if (workflow.revision) { @@ -503,7 +261,7 @@ class NfcoreSchema { // Get pipeline parameters defined in JSON Schema def Map params_summary = [:] def blacklist = ['hostnames'] - def params_map = params_load(json_schema) + def params_map = paramsLoad(getSchemaPath(workflow, schema_filename=schema_filename)) for (group in params_map.keySet()) { def sub_params = new LinkedHashMap() def group_params = params_map.get(group) // This gets the parameters of that particular group @@ -546,14 +304,14 @@ class NfcoreSchema { return [ 'Core Nextflow options' : workflow_summary ] << params_summary } - /* - * Beautify parameters for summary and return as string - */ - private static String params_summary_log(workflow, params, json_schema) { - Map colors = log_colours(params.monochrome_logs) + // + // Beautify parameters for summary and return as string + // + public static String paramsSummaryLog(workflow, params) { + Map colors = NfcoreTemplate.logColours(params.monochrome_logs) String output = '' - def params_map = params_summary_map(workflow, params, json_schema) - def max_chars = params_max_chars(params_map) + def params_map = paramsSummaryMap(workflow, params) + def max_chars = paramsMaxChars(params_map) for (group in params_map.keySet()) { def group_params = params_map.get(group) // This gets the parameters of that particular group if (group_params) { @@ -564,10 +322,196 @@ class NfcoreSchema { output += '\n' } } - output += dashed_line(params.monochrome_logs) - output += colors.dim + "\n Only displaying parameters that differ from defaults.\n" + colors.reset - output += dashed_line(params.monochrome_logs) + output += "!! Only displaying parameters that differ from the pipeline defaults !!\n" + output += NfcoreTemplate.dashedLine(params.monochrome_logs) return output } + // + // Loop over nested exceptions and print the causingException + // + private static void printExceptions(ex_json, params_json, log) { + def causingExceptions = ex_json['causingExceptions'] + if (causingExceptions.length() == 0) { + def m = ex_json['message'] =~ /required key \[([^\]]+)\] not found/ + // Missing required param + if (m.matches()) { + log.error "* Missing required parameter: --${m[0][1]}" + } + // Other base-level error + else if (ex_json['pointerToViolation'] == '#') { + log.error "* ${ex_json['message']}" + } + // Error with specific param + else { + def param = ex_json['pointerToViolation'] - ~/^#\// + def param_val = params_json[param].toString() + log.error "* --${param}: ${ex_json['message']} (${param_val})" + } + } + for (ex in causingExceptions) { + printExceptions(ex, params_json, log) + } + } + + // + // Remove an element from a JSONArray + // + private static JSONArray removeElement(json_array, element) { + def list = [] + int len = json_array.length() + for (int i=0;i + if(raw_schema.keySet().contains('definitions')){ + raw_schema.definitions.each { definition -> + for (key in definition.keySet()){ + if (definition[key].get("properties").keySet().contains(ignore_param)){ + // Remove the param to ignore + definition[key].get("properties").remove(ignore_param) + // If the param was required, change this + if (definition[key].has("required")) { + def cleaned_required = removeElement(definition[key].required, ignore_param) + definition[key].put("required", cleaned_required) + } + } + } + } + } + if(raw_schema.keySet().contains('properties') && raw_schema.get('properties').keySet().contains(ignore_param)) { + raw_schema.get("properties").remove(ignore_param) + } + if(raw_schema.keySet().contains('required') && raw_schema.required.contains(ignore_param)) { + def cleaned_required = removeElement(raw_schema.required, ignore_param) + raw_schema.put("required", cleaned_required) + } + } + return raw_schema + } + + // + // Clean and check parameters relative to Nextflow native classes + // + private static Map cleanParameters(params) { + def new_params = params.getClass().newInstance(params) + for (p in params) { + // remove anything evaluating to false + if (!p['value']) { + new_params.remove(p.key) + } + // Cast MemoryUnit to String + if (p['value'].getClass() == nextflow.util.MemoryUnit) { + new_params.replace(p.key, p['value'].toString()) + } + // Cast Duration to String + if (p['value'].getClass() == nextflow.util.Duration) { + new_params.replace(p.key, p['value'].toString().replaceFirst(/d(?!\S)/, "day")) + } + // Cast LinkedHashMap to String + if (p['value'].getClass() == LinkedHashMap) { + new_params.replace(p.key, p['value'].toString()) + } + } + return new_params + } + + // + // This function tries to read a JSON params file + // + private static LinkedHashMap paramsLoad(String json_schema) { + def params_map = new LinkedHashMap() + try { + params_map = paramsRead(json_schema) + } catch (Exception e) { + println "Could not read parameters settings from JSON. $e" + params_map = new LinkedHashMap() + } + return params_map + } + + // + // Method to actually read in JSON file using Groovy. + // Group (as Key), values are all parameters + // - Parameter1 as Key, Description as Value + // - Parameter2 as Key, Description as Value + // .... + // Group + // - + private static LinkedHashMap paramsRead(String json_schema) throws Exception { + def json = new File(json_schema).text + def Map schema_definitions = (Map) new JsonSlurper().parseText(json).get('definitions') + def Map schema_properties = (Map) new JsonSlurper().parseText(json).get('properties') + /* Tree looks like this in nf-core schema + * definitions <- this is what the first get('definitions') gets us + group 1 + title + description + properties + parameter 1 + type + description + parameter 2 + type + description + group 2 + title + description + properties + parameter 1 + type + description + * properties <- parameters can also be ungrouped, outside of definitions + parameter 1 + type + description + */ + + // Grouped params + def params_map = new LinkedHashMap() + schema_definitions.each { key, val -> + def Map group = schema_definitions."$key".properties // Gets the property object of the group + def title = schema_definitions."$key".title + def sub_params = new LinkedHashMap() + group.each { innerkey, value -> + sub_params.put(innerkey, value) + } + params_map.put(title, sub_params) + } + + // Ungrouped params + def ungrouped_params = new LinkedHashMap() + schema_properties.each { innerkey, value -> + ungrouped_params.put(innerkey, value) + } + params_map.put("Other parameters", ungrouped_params) + + return params_map + } + + // + // Get maximum number of characters across all parameter names + // + private static Integer paramsMaxChars(params_map) { + Integer max_chars = 0 + for (group in params_map.keySet()) { + def group_params = params_map.get(group) // This gets the parameters of that particular group + for (param in group_params.keySet()) { + if (param.size() > max_chars) { + max_chars = param.size() + } + } + } + return max_chars + } } diff --git a/lib/NfcoreTemplate.groovy b/lib/NfcoreTemplate.groovy new file mode 100755 index 00000000..b6e689ec --- /dev/null +++ b/lib/NfcoreTemplate.groovy @@ -0,0 +1,266 @@ +// +// This file holds several functions used within the nf-core pipeline template. +// + +import org.yaml.snakeyaml.Yaml + +class NfcoreTemplate { + + // + // Check AWS Batch related parameters have been specified correctly + // + public static void awsBatch(workflow, params) { + if (workflow.profile.contains('awsbatch')) { + // Check params.awsqueue and params.awsregion have been set if running on AWSBatch + assert (params.awsqueue && params.awsregion) : "Specify correct --awsqueue and --awsregion parameters on AWSBatch!" + // Check outdir paths to be S3 buckets if running on AWSBatch + assert params.outdir.startsWith('s3:') : "Outdir not on S3 - specify S3 Bucket to run on AWSBatch!" + } + } + + // + // Check params.hostnames + // + public static void hostName(workflow, params, log) { + Map colors = logColours(params.monochrome_logs) + if (params.hostnames) { + def hostname = "hostname".execute().text.trim() + params.hostnames.each { prof, hnames -> + hnames.each { hname -> + if (hostname.contains(hname) && !workflow.profile.contains(prof)) { + log.info "=${colors.yellow}====================================================${colors.reset}=\n" + + "${colors.yellow}WARN: You are running with `-profile $workflow.profile`\n" + + " but your machine hostname is ${colors.white}'$hostname'${colors.reset}.\n" + + " ${colors.yellow_bold}Please use `-profile $prof${colors.reset}`\n" + + "=${colors.yellow}====================================================${colors.reset}=" + } + } + } + } + } + + // + // Construct and send completion email + // + public static void email(workflow, params, summary_params, projectDir, log, multiqc_report=[]) { + + // Set up the e-mail variables + def subject = "[$workflow.manifest.name] Successful: $workflow.runName" + if (!workflow.success) { + subject = "[$workflow.manifest.name] FAILED: $workflow.runName" + } + + def summary = [:] + for (group in summary_params.keySet()) { + summary << summary_params[group] + } + + def misc_fields = [:] + misc_fields['Date Started'] = workflow.start + misc_fields['Date Completed'] = workflow.complete + misc_fields['Pipeline script file path'] = workflow.scriptFile + misc_fields['Pipeline script hash ID'] = workflow.scriptId + if (workflow.repository) misc_fields['Pipeline repository Git URL'] = workflow.repository + if (workflow.commitId) misc_fields['Pipeline repository Git Commit'] = workflow.commitId + if (workflow.revision) misc_fields['Pipeline Git branch/tag'] = workflow.revision + misc_fields['Nextflow Version'] = workflow.nextflow.version + misc_fields['Nextflow Build'] = workflow.nextflow.build + misc_fields['Nextflow Compile Timestamp'] = workflow.nextflow.timestamp + + def email_fields = [:] + email_fields['version'] = workflow.manifest.version + email_fields['runName'] = workflow.runName + email_fields['success'] = workflow.success + email_fields['dateComplete'] = workflow.complete + email_fields['duration'] = workflow.duration + email_fields['exitStatus'] = workflow.exitStatus + email_fields['errorMessage'] = (workflow.errorMessage ?: 'None') + email_fields['errorReport'] = (workflow.errorReport ?: 'None') + email_fields['commandLine'] = workflow.commandLine + email_fields['projectDir'] = workflow.projectDir + email_fields['summary'] = summary << misc_fields + + // On success try attach the multiqc report + def mqc_report = null + try { + if (workflow.success) { + mqc_report = multiqc_report.getVal() + if (mqc_report.getClass() == ArrayList && mqc_report.size() >= 1) { + if (mqc_report.size() > 1) { + log.warn "[$workflow.manifest.name] Found multiple reports from process 'MULTIQC', will use only one" + } + mqc_report = mqc_report[0] + } + } + } catch (all) { + if (multiqc_report) { + log.warn "[$workflow.manifest.name] Could not attach MultiQC report to summary email" + } + } + + // Check if we are only sending emails on failure + def email_address = params.email + if (!params.email && params.email_on_fail && !workflow.success) { + email_address = params.email_on_fail + } + + // Render the TXT template + def engine = new groovy.text.GStringTemplateEngine() + def tf = new File("$projectDir/assets/email_template.txt") + def txt_template = engine.createTemplate(tf).make(email_fields) + def email_txt = txt_template.toString() + + // Render the HTML template + def hf = new File("$projectDir/assets/email_template.html") + def html_template = engine.createTemplate(hf).make(email_fields) + def email_html = html_template.toString() + + // Render the sendmail template + def max_multiqc_email_size = params.max_multiqc_email_size as nextflow.util.MemoryUnit + def smail_fields = [ email: email_address, subject: subject, email_txt: email_txt, email_html: email_html, projectDir: "$projectDir", mqcFile: mqc_report, mqcMaxSize: max_multiqc_email_size.toBytes() ] + def sf = new File("$projectDir/assets/sendmail_template.txt") + def sendmail_template = engine.createTemplate(sf).make(smail_fields) + def sendmail_html = sendmail_template.toString() + + // Send the HTML e-mail + Map colors = logColours(params.monochrome_logs) + if (email_address) { + try { + if (params.plaintext_email) { throw GroovyException('Send plaintext e-mail, not HTML') } + // Try to send HTML e-mail using sendmail + [ 'sendmail', '-t' ].execute() << sendmail_html + log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (sendmail)-" + } catch (all) { + // Catch failures and try with plaintext + def mail_cmd = [ 'mail', '-s', subject, '--content-type=text/html', email_address ] + if ( mqc_report.size() <= max_multiqc_email_size.toBytes() ) { + mail_cmd += [ '-A', mqc_report ] + } + mail_cmd.execute() << email_html + log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (mail)-" + } + } + + // Write summary e-mail HTML to a file + def output_d = new File("${params.outdir}/pipeline_info/") + if (!output_d.exists()) { + output_d.mkdirs() + } + def output_hf = new File(output_d, "pipeline_report.html") + output_hf.withWriter { w -> w << email_html } + def output_tf = new File(output_d, "pipeline_report.txt") + output_tf.withWriter { w -> w << email_txt } + } + + // + // Print pipeline summary on completion + // + public static void summary(workflow, params, log) { + Map colors = logColours(params.monochrome_logs) + if (workflow.success) { + if (workflow.stats.ignoredCount == 0) { + log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Pipeline completed successfully${colors.reset}-" + } else { + log.info "-${colors.purple}[$workflow.manifest.name]${colors.red} Pipeline completed successfully, but with errored process(es) ${colors.reset}-" + } + } else { + hostName(workflow, params, log) + log.info "-${colors.purple}[$workflow.manifest.name]${colors.red} Pipeline completed with errors${colors.reset}-" + } + } + + // + // ANSII Colours used for terminal logging + // + public static Map logColours(Boolean monochrome_logs) { + Map colorcodes = [:] + + // Reset / Meta + colorcodes['reset'] = monochrome_logs ? '' : "\033[0m" + colorcodes['bold'] = monochrome_logs ? '' : "\033[1m" + colorcodes['dim'] = monochrome_logs ? '' : "\033[2m" + colorcodes['underlined'] = monochrome_logs ? '' : "\033[4m" + colorcodes['blink'] = monochrome_logs ? '' : "\033[5m" + colorcodes['reverse'] = monochrome_logs ? '' : "\033[7m" + colorcodes['hidden'] = monochrome_logs ? '' : "\033[8m" + + // Regular Colors + colorcodes['black'] = monochrome_logs ? '' : "\033[0;30m" + colorcodes['red'] = monochrome_logs ? '' : "\033[0;31m" + colorcodes['green'] = monochrome_logs ? '' : "\033[0;32m" + colorcodes['yellow'] = monochrome_logs ? '' : "\033[0;33m" + colorcodes['blue'] = monochrome_logs ? '' : "\033[0;34m" + colorcodes['purple'] = monochrome_logs ? '' : "\033[0;35m" + colorcodes['cyan'] = monochrome_logs ? '' : "\033[0;36m" + colorcodes['white'] = monochrome_logs ? '' : "\033[0;37m" + + // Bold + colorcodes['bblack'] = monochrome_logs ? '' : "\033[1;30m" + colorcodes['bred'] = monochrome_logs ? '' : "\033[1;31m" + colorcodes['bgreen'] = monochrome_logs ? '' : "\033[1;32m" + colorcodes['byellow'] = monochrome_logs ? '' : "\033[1;33m" + colorcodes['bblue'] = monochrome_logs ? '' : "\033[1;34m" + colorcodes['bpurple'] = monochrome_logs ? '' : "\033[1;35m" + colorcodes['bcyan'] = monochrome_logs ? '' : "\033[1;36m" + colorcodes['bwhite'] = monochrome_logs ? '' : "\033[1;37m" + + // Underline + colorcodes['ublack'] = monochrome_logs ? '' : "\033[4;30m" + colorcodes['ured'] = monochrome_logs ? '' : "\033[4;31m" + colorcodes['ugreen'] = monochrome_logs ? '' : "\033[4;32m" + colorcodes['uyellow'] = monochrome_logs ? '' : "\033[4;33m" + colorcodes['ublue'] = monochrome_logs ? '' : "\033[4;34m" + colorcodes['upurple'] = monochrome_logs ? '' : "\033[4;35m" + colorcodes['ucyan'] = monochrome_logs ? '' : "\033[4;36m" + colorcodes['uwhite'] = monochrome_logs ? '' : "\033[4;37m" + + // High Intensity + colorcodes['iblack'] = monochrome_logs ? '' : "\033[0;90m" + colorcodes['ired'] = monochrome_logs ? '' : "\033[0;91m" + colorcodes['igreen'] = monochrome_logs ? '' : "\033[0;92m" + colorcodes['iyellow'] = monochrome_logs ? '' : "\033[0;93m" + colorcodes['iblue'] = monochrome_logs ? '' : "\033[0;94m" + colorcodes['ipurple'] = monochrome_logs ? '' : "\033[0;95m" + colorcodes['icyan'] = monochrome_logs ? '' : "\033[0;96m" + colorcodes['iwhite'] = monochrome_logs ? '' : "\033[0;97m" + + // Bold High Intensity + colorcodes['biblack'] = monochrome_logs ? '' : "\033[1;90m" + colorcodes['bired'] = monochrome_logs ? '' : "\033[1;91m" + colorcodes['bigreen'] = monochrome_logs ? '' : "\033[1;92m" + colorcodes['biyellow'] = monochrome_logs ? '' : "\033[1;93m" + colorcodes['biblue'] = monochrome_logs ? '' : "\033[1;94m" + colorcodes['bipurple'] = monochrome_logs ? '' : "\033[1;95m" + colorcodes['bicyan'] = monochrome_logs ? '' : "\033[1;96m" + colorcodes['biwhite'] = monochrome_logs ? '' : "\033[1;97m" + + return colorcodes + } + + // + // Does what is says on the tin + // + public static String dashedLine(monochrome_logs) { + Map colors = logColours(monochrome_logs) + return "-${colors.dim}----------------------------------------------------${colors.reset}-" + } + + // + // nf-core logo + // + public static String logo(workflow, monochrome_logs) { + Map colors = logColours(monochrome_logs) + String.format( + """\n + ${dashedLine(monochrome_logs)} + ${colors.green},--.${colors.black}/${colors.green},-.${colors.reset} + ${colors.blue} ___ __ __ __ ___ ${colors.green}/,-._.--~\'${colors.reset} + ${colors.blue} |\\ | |__ __ / ` / \\ |__) |__ ${colors.yellow}} {${colors.reset} + ${colors.blue} | \\| | \\__, \\__/ | \\ |___ ${colors.green}\\`-._,-`-,${colors.reset} + ${colors.green}`._,._,\'${colors.reset} + ${colors.purple} ${workflow.manifest.name} v${workflow.manifest.version}${colors.reset} + ${dashedLine(monochrome_logs)} + """.stripIndent() + ) + } +} diff --git a/lib/Utils.groovy b/lib/Utils.groovy new file mode 100755 index 00000000..18173e98 --- /dev/null +++ b/lib/Utils.groovy @@ -0,0 +1,47 @@ +// +// This file holds several Groovy functions that could be useful for any Nextflow pipeline +// + +import org.yaml.snakeyaml.Yaml + +class Utils { + + // + // When running with -profile conda, warn if channels have not been set-up appropriately + // + public static void checkCondaChannels(log) { + Yaml parser = new Yaml() + def channels = [] + try { + def config = parser.load("conda config --show channels".execute().text) + channels = config.channels + } catch(NullPointerException | IOException e) { + log.warn "Could not verify conda channel configuration." + return + } + + // Check that all channels are present + def required_channels = ['conda-forge', 'bioconda', 'defaults'] + def conda_check_failed = !required_channels.every { ch -> ch in channels } + + // Check that they are in the right order + conda_check_failed |= !(channels.indexOf('conda-forge') < channels.indexOf('bioconda')) + conda_check_failed |= !(channels.indexOf('bioconda') < channels.indexOf('defaults')) + + if (conda_check_failed) { + log.warn "=============================================================================\n" + + " There is a problem with your Conda configuration!\n\n" + + " You will need to set-up the conda-forge and bioconda channels correctly.\n" + + " Please refer to https://bioconda.github.io/user/install.html#set-up-channels\n" + + " NB: The order of the channels matters!\n" + + "===================================================================================" + } + } + + // + // Join module args with appropriate spacing + // + public static String joinModuleArgs(args_list) { + return ' ' + args_list.join(' ') + } +} diff --git a/lib/WorkflowBacass.groovy b/lib/WorkflowBacass.groovy new file mode 100755 index 00000000..94f7e96d --- /dev/null +++ b/lib/WorkflowBacass.groovy @@ -0,0 +1,59 @@ +// +// This file holds several functions specific to the workflow/bacass.nf in the nf-core/bacass pipeline +// + +class WorkflowBacass { + + // + // Check and validate parameters + // + public static void initialise(params, log) { + genomeExistsError(params, log) + + if (!params.fasta) { + log.error "Genome fasta file not specified with e.g. '--fasta genome.fa' or via a detectable config file." + System.exit(1) + } + } + + // + // Get workflow summary for MultiQC + // + public static String paramsSummaryMultiqc(workflow, summary) { + String summary_section = '' + for (group in summary.keySet()) { + def group_params = summary.get(group) // This gets the parameters of that particular group + if (group_params) { + summary_section += "

$group

\n" + summary_section += "
\n" + } + } + + String yaml_file_text = "id: '${workflow.manifest.name.replace('/','-')}-summary'\n" + yaml_file_text += "description: ' - this information is collected when the pipeline is started.'\n" + yaml_file_text += "section_name: '${workflow.manifest.name} Workflow Summary'\n" + yaml_file_text += "section_href: 'https://github.com/${workflow.manifest.name}'\n" + yaml_file_text += "plot_type: 'html'\n" + yaml_file_text += "data: |\n" + yaml_file_text += "${summary_section}" + return yaml_file_text + } + + // + // Exit pipeline if incorrect --genome key provided + // + private static void genomeExistsError(params, log) { + if (params.genomes && params.genome && !params.genomes.containsKey(params.genome)) { + log.error "=============================================================================\n" + + " Genome '${params.genome}' not found in any config files provided to the pipeline.\n" + + " Currently, the available genome keys are:\n" + + " ${params.genomes.keySet().join(", ")}\n" + + "===================================================================================" + System.exit(1) + } + } +} diff --git a/lib/WorkflowMain.groovy b/lib/WorkflowMain.groovy new file mode 100755 index 00000000..6e61e6a5 --- /dev/null +++ b/lib/WorkflowMain.groovy @@ -0,0 +1,94 @@ +// +// This file holds several functions specific to the main.nf workflow in the nf-core/bacass pipeline +// + +class WorkflowMain { + + // + // Citation string for pipeline + // + public static String citation(workflow) { + return "If you use ${workflow.manifest.name} for your analysis please cite:\n\n" + + // TODO nf-core: Add Zenodo DOI for pipeline after first release + //"* The pipeline\n" + + //" https://doi.org/10.5281/zenodo.XXXXXXX\n\n" + + "* The nf-core framework\n" + + " https://doi.org/10.1038/s41587-020-0439-x\n\n" + + "* Software dependencies\n" + + " https://github.com/${workflow.manifest.name}/blob/master/CITATIONS.md" + } + + // + // Print help to screen if required + // + public static String help(workflow, params, log) { + def command = "nextflow run ${workflow.manifest.name} --input samplesheet.csv --genome GRCh37 -profile docker" + def help_string = '' + help_string += NfcoreTemplate.logo(workflow, params.monochrome_logs) + help_string += NfcoreSchema.paramsHelp(workflow, params, command) + help_string += '\n' + citation(workflow) + '\n' + help_string += NfcoreTemplate.dashedLine(params.monochrome_logs) + return help_string + } + + // + // Print parameter summary log to screen + // + public static String paramsSummaryLog(workflow, params, log) { + def summary_log = '' + summary_log += NfcoreTemplate.logo(workflow, params.monochrome_logs) + summary_log += NfcoreSchema.paramsSummaryLog(workflow, params) + summary_log += '\n' + citation(workflow) + '\n' + summary_log += NfcoreTemplate.dashedLine(params.monochrome_logs) + return summary_log + } + + // + // Validate parameters and print summary to screen + // + public static void initialise(workflow, params, log) { + // Print help to screen if required + if (params.help) { + log.info help(workflow, params, log) + System.exit(0) + } + + // Validate workflow parameters via the JSON schema + if (params.validate_params) { + NfcoreSchema.validateParameters(workflow, params, log) + } + + // Print parameter summary log to screen + log.info paramsSummaryLog(workflow, params, log) + + // Check that conda channels are set-up correctly + if (params.enable_conda) { + Utils.checkCondaChannels(log) + } + + // Check AWS batch settings + NfcoreTemplate.awsBatch(workflow, params) + + // Check the hostnames against configured profiles + NfcoreTemplate.hostName(workflow, params, log) + + // Check input has been provided + if (!params.input) { + log.error "Please provide an input samplesheet to the pipeline e.g. '--input samplesheet.csv'" + System.exit(1) + } + } + + // + // Get attribute from genome config file e.g. fasta + // + public static String getGenomeAttribute(params, attribute) { + def val = '' + if (params.genomes && params.genome && params.genomes.containsKey(params.genome)) { + if (params.genomes[ params.genome ].containsKey(attribute)) { + val = params.genomes[ params.genome ][ attribute ] + } + } + return val + } +} diff --git a/main.nf b/main.nf index e15f2b3a..09ddaab9 100644 --- a/main.nf +++ b/main.nf @@ -1,390 +1,63 @@ #!/usr/bin/env nextflow /* ======================================================================================== - nf-core/bacass + nf-core/bacass ======================================================================================== - nf-core/bacass Analysis Pipeline. - #### Homepage / Documentation - https://github.com/nf-core/bacass + Github : https://github.com/nf-core/bacass + Website: https://nf-co.re/bacass + Slack : https://nfcore.slack.com/channels/bacass ---------------------------------------------------------------------------------------- */ -log.info Headers.nf_core(workflow, params.monochrome_logs) - -//////////////////////////////////////////////////// -/* -- PRINT HELP -- */ -////////////////////////////////////////////////////+ -def json_schema = "$projectDir/nextflow_schema.json" -if (params.help) { - def command = "nextflow run nf-core/bacass --input '*_R{1,2}.fastq.gz' -profile docker" - log.info NfcoreSchema.params_help(workflow, params, json_schema, command) - exit 0 -} - -//////////////////////////////////////////////////// -/* -- VALIDATE PARAMETERS -- */ -////////////////////////////////////////////////////+ -if (params.validate_params) { - NfcoreSchema.validateParameters(params, json_schema, log) -} - -//////////////////////////////////////////////////// -/* -- Collect configuration parameters -- */ -//////////////////////////////////////////////////// - -// Check if genome exists in the config file -if (params.genomes && params.genome && !params.genomes.containsKey(params.genome)) { - exit 1, "The provided genome '${params.genome}' is not available in the iGenomes file. Currently the available genomes are ${params.genomes.keySet().join(', ')}" -} - -// TODO nf-core: Add any reference files that are needed -// Configurable reference genomes -// -// NOTE - THIS IS NOT USED IN THIS PIPELINE, EXAMPLE ONLY -// If you want to use the channel below in a process, define the following: -// input: -// file fasta from ch_fasta -// -params.fasta = params.genome ? params.genomes[ params.genome ].fasta ?: false : false -if (params.fasta) { ch_fasta = file(params.fasta, checkIfExists: true) } - -// Check AWS batch settings -if (workflow.profile.contains('awsbatch')) { - // AWSBatch sanity checking - if (!params.awsqueue || !params.awsregion) exit 1, 'Specify correct --awsqueue and --awsregion parameters on AWSBatch!' - // Check outdir paths to be S3 buckets if running on AWSBatch - // related: https://github.com/nextflow-io/nextflow/issues/813 - if (!params.outdir.startsWith('s3:')) exit 1, 'Outdir not on S3 - specify S3 Bucket to run on AWSBatch!' - // Prevent trace files to be stored on S3 since S3 does not support rolling files. - if (params.tracedir.startsWith('s3:')) exit 1, 'Specify a local tracedir or run without trace! S3 cannot be used for tracefiles.' -} - -// Stage config files -ch_multiqc_config = file("$projectDir/assets/multiqc_config.yaml", checkIfExists: true) -ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath(params.multiqc_config, checkIfExists: true) : Channel.empty() -ch_output_docs = file("$projectDir/docs/output.md", checkIfExists: true) -ch_output_docs_images = file("$projectDir/docs/images/", checkIfExists: true) +nextflow.enable.dsl = 2 /* - * Create a channel for input read files - */ -if (params.input_paths) { - if (params.single_end) { - Channel - .from(params.input_paths) - .map { row -> [ row[0], [ file(row[1][0], checkIfExists: true) ] ] } - .ifEmpty { exit 1, 'params.input_paths was empty - no input files supplied' } - .into { ch_read_files_fastqc; ch_read_files_trimming } - } else { - Channel - .from(params.input_paths) - .map { row -> [ row[0], [ file(row[1][0], checkIfExists: true), file(row[1][1], checkIfExists: true) ] ] } - .ifEmpty { exit 1, 'params.input_paths was empty - no input files supplied' } - .into { ch_read_files_fastqc; ch_read_files_trimming } - } -} else { - Channel - .fromFilePairs(params.input, size: params.single_end ? 1 : 2) - .ifEmpty { exit 1, "Cannot find any reads matching: ${params.input}\nNB: Path needs to be enclosed in quotes!\nIf this is single-end data, please specify --single_end on the command line." } - .into { ch_read_files_fastqc; ch_read_files_trimming } -} - -//////////////////////////////////////////////////// -/* -- PRINT PARAMETER SUMMARY -- */ -//////////////////////////////////////////////////// -log.info NfcoreSchema.params_summary_log(workflow, params, json_schema) - -// Header log info -def summary = [:] -if (workflow.revision) summary['Pipeline Release'] = workflow.revision -summary['Run Name'] = workflow.runName -// TODO nf-core: Report custom parameters here -summary['Input'] = params.input -summary['Fasta Ref'] = params.fasta -summary['Data Type'] = params.single_end ? 'Single-End' : 'Paired-End' -summary['Max Resources'] = "$params.max_memory memory, $params.max_cpus cpus, $params.max_time time per job" -if (workflow.containerEngine) summary['Container'] = "$workflow.containerEngine - $workflow.container" -summary['Output dir'] = params.outdir -summary['Launch dir'] = workflow.launchDir -summary['Working dir'] = workflow.workDir -summary['Script dir'] = workflow.projectDir -summary['User'] = workflow.userName -if (workflow.profile.contains('awsbatch')) { - summary['AWS Region'] = params.awsregion - summary['AWS Queue'] = params.awsqueue - summary['AWS CLI'] = params.awscli -} -summary['Config Profile'] = workflow.profile -if (params.config_profile_description) summary['Config Profile Description'] = params.config_profile_description -if (params.config_profile_contact) summary['Config Profile Contact'] = params.config_profile_contact -if (params.config_profile_url) summary['Config Profile URL'] = params.config_profile_url -summary['Config Files'] = workflow.configFiles.join(', ') -if (params.email || params.email_on_fail) { - summary['E-mail Address'] = params.email - summary['E-mail on failure'] = params.email_on_fail - summary['MultiQC maxsize'] = params.max_multiqc_email_size -} - -// Check the hostnames against configured profiles -checkHostname() - -Channel.from(summary.collect{ [it.key, it.value] }) - .map { k,v -> "
$k
${v ?: 'N/A'}
" } - .reduce { a, b -> return [a, b].join("\n ") } - .map { x -> """ - id: 'nf-core-bacass-summary' - description: " - this information is collected when the pipeline is started." - section_name: 'nf-core/bacass Workflow Summary' - section_href: 'https://github.com/nf-core/bacass' - plot_type: 'html' - data: | -
- $x -
- """.stripIndent() } - .set { ch_workflow_summary } - -/* - * Parse software version numbers - */ -process get_software_versions { - publishDir "${params.outdir}/pipeline_info", mode: params.publish_dir_mode, - saveAs: { filename -> - if (filename.indexOf('.csv') > 0) filename - else null - } - - output: - file 'software_versions_mqc.yaml' into ch_software_versions_yaml - file 'software_versions.csv' +======================================================================================== + GENOME PARAMETER VALUES +======================================================================================== +*/ - script: - // TODO nf-core: Get all tools to print their version number here - """ - echo $workflow.manifest.version > v_pipeline.txt - echo $workflow.nextflow.version > v_nextflow.txt - fastqc --version > v_fastqc.txt - multiqc --version > v_multiqc.txt - scrape_software_versions.py &> software_versions_mqc.yaml - """ -} +params.fasta = WorkflowMain.getGenomeAttribute(params, 'fasta') /* - * STEP 1 - FastQC - */ -process fastqc { - tag "$name" - label 'process_medium' - publishDir "${params.outdir}/fastqc", mode: params.publish_dir_mode, - saveAs: { filename -> - filename.indexOf('.zip') > 0 ? "zips/$filename" : "$filename" - } - - input: - set val(name), file(reads) from ch_read_files_fastqc - - output: - file '*_fastqc.{zip,html}' into ch_fastqc_results +======================================================================================== + VALIDATE & PRINT PARAMETER SUMMARY +======================================================================================== +*/ - script: - """ - fastqc --quiet --threads $task.cpus $reads - """ -} +WorkflowMain.initialise(workflow, params, log) /* - * STEP 2 - MultiQC - */ -process multiqc { - publishDir "${params.outdir}/MultiQC", mode: params.publish_dir_mode - - input: - file (multiqc_config) from ch_multiqc_config - file (mqc_custom_config) from ch_multiqc_custom_config.collect().ifEmpty([]) - // TODO nf-core: Add in log files from your new processes for MultiQC to find! - file ('fastqc/*') from ch_fastqc_results.collect().ifEmpty([]) - file ('software_versions/*') from ch_software_versions_yaml.collect() - file workflow_summary from ch_workflow_summary.collectFile(name: "workflow_summary_mqc.yaml") +======================================================================================== + NAMED WORKFLOW FOR PIPELINE +======================================================================================== +*/ - output: - file "*multiqc_report.html" into ch_multiqc_report - file "*_data" - file "multiqc_plots" +include { BACASS } from './workflows/bacass' - script: - rtitle = '' - rfilename = '' - if (!(workflow.runName ==~ /[a-z]+_[a-z]+/)) { - rtitle = "--title \"${workflow.runName}\"" - rfilename = "--filename " + workflow.runName.replaceAll('\\W','_').replaceAll('_+','_') + "_multiqc_report" - } - custom_config_file = params.multiqc_config ? "--config $mqc_custom_config" : '' - // TODO nf-core: Specify which MultiQC modules to use with -m for a faster run time - """ - multiqc -f $rtitle $rfilename $custom_config_file . - """ +// +// WORKFLOW: Run main nf-core/bacass analysis pipeline +// +workflow NFCORE_BACASS { + BACASS () } /* - * STEP 3 - Output Description HTML - */ -process output_documentation { - publishDir "${params.outdir}/pipeline_info", mode: params.publish_dir_mode - - input: - file output_docs from ch_output_docs - file images from ch_output_docs_images - - output: - file 'results_description.html' +======================================================================================== + RUN ALL WORKFLOWS +======================================================================================== +*/ - script: - """ - markdown_to_html.py $output_docs -o results_description.html - """ +// +// WORKFLOW: Execute a single named workflow for the pipeline +// See: https://github.com/nf-core/rnaseq/issues/619 +// +workflow { + NFCORE_BACASS () } /* - * Completion e-mail notification - */ -workflow.onComplete { - - // Set up the e-mail variables - def subject = "[nf-core/bacass] Successful: $workflow.runName" - if (!workflow.success) { - subject = "[nf-core/bacass] FAILED: $workflow.runName" - } - def email_fields = [:] - email_fields['version'] = workflow.manifest.version - email_fields['runName'] = workflow.runName - email_fields['success'] = workflow.success - email_fields['dateComplete'] = workflow.complete - email_fields['duration'] = workflow.duration - email_fields['exitStatus'] = workflow.exitStatus - email_fields['errorMessage'] = (workflow.errorMessage ?: 'None') - email_fields['errorReport'] = (workflow.errorReport ?: 'None') - email_fields['commandLine'] = workflow.commandLine - email_fields['projectDir'] = workflow.projectDir - email_fields['summary'] = summary - email_fields['summary']['Date Started'] = workflow.start - email_fields['summary']['Date Completed'] = workflow.complete - email_fields['summary']['Pipeline script file path'] = workflow.scriptFile - email_fields['summary']['Pipeline script hash ID'] = workflow.scriptId - if (workflow.repository) email_fields['summary']['Pipeline repository Git URL'] = workflow.repository - if (workflow.commitId) email_fields['summary']['Pipeline repository Git Commit'] = workflow.commitId - if (workflow.revision) email_fields['summary']['Pipeline Git branch/tag'] = workflow.revision - email_fields['summary']['Nextflow Version'] = workflow.nextflow.version - email_fields['summary']['Nextflow Build'] = workflow.nextflow.build - email_fields['summary']['Nextflow Compile Timestamp'] = workflow.nextflow.timestamp - - // TODO nf-core: If not using MultiQC, strip out this code (including params.max_multiqc_email_size) - // On success try attach the multiqc report - def mqc_report = null - try { - if (workflow.success) { - mqc_report = ch_multiqc_report.getVal() - if (mqc_report.getClass() == ArrayList) { - log.warn "[nf-core/bacass] Found multiple reports from process 'multiqc', will use only one" - mqc_report = mqc_report[0] - } - } - } catch (all) { - log.warn "[nf-core/bacass] Could not attach MultiQC report to summary email" - } - - // Check if we are only sending emails on failure - email_address = params.email - if (!params.email && params.email_on_fail && !workflow.success) { - email_address = params.email_on_fail - } - - // Render the TXT template - def engine = new groovy.text.GStringTemplateEngine() - def tf = new File("$projectDir/assets/email_template.txt") - def txt_template = engine.createTemplate(tf).make(email_fields) - def email_txt = txt_template.toString() - - // Render the HTML template - def hf = new File("$projectDir/assets/email_template.html") - def html_template = engine.createTemplate(hf).make(email_fields) - def email_html = html_template.toString() - - // Render the sendmail template - def smail_fields = [ email: email_address, subject: subject, email_txt: email_txt, email_html: email_html, projectDir: "$projectDir", mqcFile: mqc_report, mqcMaxSize: params.max_multiqc_email_size.toBytes() ] - def sf = new File("$projectDir/assets/sendmail_template.txt") - def sendmail_template = engine.createTemplate(sf).make(smail_fields) - def sendmail_html = sendmail_template.toString() - - // Send the HTML e-mail - if (email_address) { - try { - if (params.plaintext_email) { throw GroovyException('Send plaintext e-mail, not HTML') } - // Try to send HTML e-mail using sendmail - [ 'sendmail', '-t' ].execute() << sendmail_html - log.info "[nf-core/bacass] Sent summary e-mail to $email_address (sendmail)" - } catch (all) { - // Catch failures and try with plaintext - def mail_cmd = [ 'mail', '-s', subject, '--content-type=text/html', email_address ] - if ( mqc_report.size() <= params.max_multiqc_email_size.toBytes() ) { - mail_cmd += [ '-A', mqc_report ] - } - mail_cmd.execute() << email_html - log.info "[nf-core/bacass] Sent summary e-mail to $email_address (mail)" - } - } - - // Write summary e-mail HTML to a file - def output_d = new File("${params.outdir}/pipeline_info/") - if (!output_d.exists()) { - output_d.mkdirs() - } - def output_hf = new File(output_d, "pipeline_report.html") - output_hf.withWriter { w -> w << email_html } - def output_tf = new File(output_d, "pipeline_report.txt") - output_tf.withWriter { w -> w << email_txt } - - c_green = params.monochrome_logs ? '' : "\033[0;32m"; - c_purple = params.monochrome_logs ? '' : "\033[0;35m"; - c_red = params.monochrome_logs ? '' : "\033[0;31m"; - c_reset = params.monochrome_logs ? '' : "\033[0m"; - - if (workflow.stats.ignoredCount > 0 && workflow.success) { - log.info "-${c_purple}Warning, pipeline completed, but with errored process(es) ${c_reset}-" - log.info "-${c_red}Number of ignored errored process(es) : ${workflow.stats.ignoredCount} ${c_reset}-" - log.info "-${c_green}Number of successfully ran process(es) : ${workflow.stats.succeedCount} ${c_reset}-" - } - - if (workflow.success) { - log.info "-${c_purple}[nf-core/bacass]${c_green} Pipeline completed successfully${c_reset}-" - } else { - checkHostname() - log.info "-${c_purple}[nf-core/bacass]${c_red} Pipeline completed with errors${c_reset}-" - } - -} - -workflow.onError { - // Print unexpected parameters - easiest is to just rerun validation - NfcoreSchema.validateParameters(params, json_schema, log) -} - -def checkHostname() { - def c_reset = params.monochrome_logs ? '' : "\033[0m" - def c_white = params.monochrome_logs ? '' : "\033[0;37m" - def c_red = params.monochrome_logs ? '' : "\033[1;91m" - def c_yellow_bold = params.monochrome_logs ? '' : "\033[1;93m" - if (params.hostnames) { - def hostname = 'hostname'.execute().text.trim() - params.hostnames.each { prof, hnames -> - hnames.each { hname -> - if (hostname.contains(hname) && !workflow.profile.contains(prof)) { - log.error "${c_red}====================================================${c_reset}\n" + - " ${c_red}WARNING!${c_reset} You are running with `-profile $workflow.profile`\n" + - " but your machine hostname is ${c_white}'$hostname'${c_reset}\n" + - " ${c_yellow_bold}It's highly recommended that you use `-profile $prof${c_reset}`\n" + - "${c_red}====================================================${c_reset}\n" - } - } - } - } -} +======================================================================================== + THE END +======================================================================================== +*/ diff --git a/modules.json b/modules.json new file mode 100644 index 00000000..7d548cca --- /dev/null +++ b/modules.json @@ -0,0 +1,14 @@ +{ + "name": "nf-core/bacass", + "homePage": "https://github.com/nf-core/bacass", + "repos": { + "nf-core/modules": { + "fastqc": { + "git_sha": "e937c7950af70930d1f34bb961403d9d2aa81c7d" + }, + "multiqc": { + "git_sha": "e937c7950af70930d1f34bb961403d9d2aa81c7d" + } + } + } +} diff --git a/modules/local/functions.nf b/modules/local/functions.nf new file mode 100644 index 00000000..da9da093 --- /dev/null +++ b/modules/local/functions.nf @@ -0,0 +1,68 @@ +// +// Utility functions used in nf-core DSL2 module files +// + +// +// Extract name of software tool from process name using $task.process +// +def getSoftwareName(task_process) { + return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() +} + +// +// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules +// +def initOptions(Map args) { + def Map options = [:] + options.args = args.args ?: '' + options.args2 = args.args2 ?: '' + options.args3 = args.args3 ?: '' + options.publish_by_meta = args.publish_by_meta ?: [] + options.publish_dir = args.publish_dir ?: '' + options.publish_files = args.publish_files + options.suffix = args.suffix ?: '' + return options +} + +// +// Tidy up and join elements of a list to return a path string +// +def getPathFromList(path_list) { + def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries + paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes + return paths.join('/') +} + +// +// Function to save/publish module results +// +def saveFiles(Map args) { + if (!args.filename.endsWith('.version.txt')) { + def ioptions = initOptions(args.options) + def path_list = [ ioptions.publish_dir ?: args.publish_dir ] + if (ioptions.publish_by_meta) { + def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta + for (key in key_list) { + if (args.meta && key instanceof String) { + def path = key + if (args.meta.containsKey(key)) { + path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] + } + path = path instanceof String ? path : '' + path_list.add(path) + } + } + } + if (ioptions.publish_files instanceof Map) { + for (ext in ioptions.publish_files) { + if (args.filename.endsWith(ext.key)) { + def ext_list = path_list.collect() + ext_list.add(ext.value) + return "${getPathFromList(ext_list)}/$args.filename" + } + } + } else if (ioptions.publish_files == null) { + return "${getPathFromList(path_list)}/$args.filename" + } + } +} diff --git a/modules/local/get_software_versions.nf b/modules/local/get_software_versions.nf new file mode 100644 index 00000000..d7a9a92e --- /dev/null +++ b/modules/local/get_software_versions.nf @@ -0,0 +1,33 @@ +// Import generic module functions +include { saveFiles } from './functions' + +params.options = [:] + +process GET_SOFTWARE_VERSIONS { + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:'pipeline_info', meta:[:], publish_by_meta:[]) } + + conda (params.enable_conda ? "conda-forge::python=3.8.3" : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://depot.galaxyproject.org/singularity/python:3.8.3" + } else { + container "quay.io/biocontainers/python:3.8.3" + } + + cache false + + input: + path versions + + output: + path "software_versions.tsv" , emit: tsv + path 'software_versions_mqc.yaml', emit: yaml + + script: // This script is bundled with the pipeline, in nf-core/bacass/bin/ + """ + echo $workflow.manifest.version > pipeline.version.txt + echo $workflow.nextflow.version > nextflow.version.txt + scrape_software_versions.py &> software_versions_mqc.yaml + """ +} diff --git a/modules/local/samplesheet_check.nf b/modules/local/samplesheet_check.nf new file mode 100644 index 00000000..e5ba91c2 --- /dev/null +++ b/modules/local/samplesheet_check.nf @@ -0,0 +1,31 @@ +// Import generic module functions +include { saveFiles } from './functions' + +params.options = [:] + +process SAMPLESHEET_CHECK { + tag "$samplesheet" + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:'pipeline_info', meta:[:], publish_by_meta:[]) } + + conda (params.enable_conda ? "conda-forge::python=3.8.3" : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://depot.galaxyproject.org/singularity/python:3.8.3" + } else { + container "quay.io/biocontainers/python:3.8.3" + } + + input: + path samplesheet + + output: + path '*.csv' + + script: // This script is bundled with the pipeline, in nf-core/bacass/bin/ + """ + check_samplesheet.py \\ + $samplesheet \\ + samplesheet.valid.csv + """ +} diff --git a/modules/nf-core/modules/fastqc/functions.nf b/modules/nf-core/modules/fastqc/functions.nf new file mode 100644 index 00000000..da9da093 --- /dev/null +++ b/modules/nf-core/modules/fastqc/functions.nf @@ -0,0 +1,68 @@ +// +// Utility functions used in nf-core DSL2 module files +// + +// +// Extract name of software tool from process name using $task.process +// +def getSoftwareName(task_process) { + return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() +} + +// +// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules +// +def initOptions(Map args) { + def Map options = [:] + options.args = args.args ?: '' + options.args2 = args.args2 ?: '' + options.args3 = args.args3 ?: '' + options.publish_by_meta = args.publish_by_meta ?: [] + options.publish_dir = args.publish_dir ?: '' + options.publish_files = args.publish_files + options.suffix = args.suffix ?: '' + return options +} + +// +// Tidy up and join elements of a list to return a path string +// +def getPathFromList(path_list) { + def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries + paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes + return paths.join('/') +} + +// +// Function to save/publish module results +// +def saveFiles(Map args) { + if (!args.filename.endsWith('.version.txt')) { + def ioptions = initOptions(args.options) + def path_list = [ ioptions.publish_dir ?: args.publish_dir ] + if (ioptions.publish_by_meta) { + def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta + for (key in key_list) { + if (args.meta && key instanceof String) { + def path = key + if (args.meta.containsKey(key)) { + path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] + } + path = path instanceof String ? path : '' + path_list.add(path) + } + } + } + if (ioptions.publish_files instanceof Map) { + for (ext in ioptions.publish_files) { + if (args.filename.endsWith(ext.key)) { + def ext_list = path_list.collect() + ext_list.add(ext.value) + return "${getPathFromList(ext_list)}/$args.filename" + } + } + } else if (ioptions.publish_files == null) { + return "${getPathFromList(path_list)}/$args.filename" + } + } +} diff --git a/modules/nf-core/modules/fastqc/main.nf b/modules/nf-core/modules/fastqc/main.nf new file mode 100644 index 00000000..39c327b2 --- /dev/null +++ b/modules/nf-core/modules/fastqc/main.nf @@ -0,0 +1,47 @@ +// Import generic module functions +include { initOptions; saveFiles; getSoftwareName } from './functions' + +params.options = [:] +options = initOptions(params.options) + +process FASTQC { + tag "$meta.id" + label 'process_medium' + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } + + conda (params.enable_conda ? "bioconda::fastqc=0.11.9" : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://depot.galaxyproject.org/singularity/fastqc:0.11.9--0" + } else { + container "quay.io/biocontainers/fastqc:0.11.9--0" + } + + input: + tuple val(meta), path(reads) + + output: + tuple val(meta), path("*.html"), emit: html + tuple val(meta), path("*.zip") , emit: zip + path "*.version.txt" , emit: version + + script: + // Add soft-links to original FastQs for consistent naming in pipeline + def software = getSoftwareName(task.process) + def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" + if (meta.single_end) { + """ + [ ! -f ${prefix}.fastq.gz ] && ln -s $reads ${prefix}.fastq.gz + fastqc $options.args --threads $task.cpus ${prefix}.fastq.gz + fastqc --version | sed -e "s/FastQC v//g" > ${software}.version.txt + """ + } else { + """ + [ ! -f ${prefix}_1.fastq.gz ] && ln -s ${reads[0]} ${prefix}_1.fastq.gz + [ ! -f ${prefix}_2.fastq.gz ] && ln -s ${reads[1]} ${prefix}_2.fastq.gz + fastqc $options.args --threads $task.cpus ${prefix}_1.fastq.gz ${prefix}_2.fastq.gz + fastqc --version | sed -e "s/FastQC v//g" > ${software}.version.txt + """ + } +} diff --git a/modules/nf-core/modules/fastqc/meta.yml b/modules/nf-core/modules/fastqc/meta.yml new file mode 100644 index 00000000..8eb9953d --- /dev/null +++ b/modules/nf-core/modules/fastqc/meta.yml @@ -0,0 +1,51 @@ +name: fastqc +description: Run FastQC on sequenced reads +keywords: + - quality control + - qc + - adapters + - fastq +tools: + - fastqc: + description: | + FastQC gives general quality metrics about your reads. + It provides information about the quality score distribution + across your reads, the per base sequence content (%A/C/G/T). + You get information about adapter contamination and other + overrepresented sequences. + homepage: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/ + documentation: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/Help/ +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - reads: + type: file + description: | + List of input FastQ files of size 1 and 2 for single-end and paired-end data, + respectively. +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - html: + type: file + description: FastQC report + pattern: "*_{fastqc.html}" + - zip: + type: file + description: FastQC report archive + pattern: "*_{fastqc.zip}" + - version: + type: file + description: File containing software version + pattern: "*.{version.txt}" +authors: + - "@drpatelh" + - "@grst" + - "@ewels" + - "@FelixKrueger" diff --git a/modules/nf-core/modules/multiqc/functions.nf b/modules/nf-core/modules/multiqc/functions.nf new file mode 100644 index 00000000..da9da093 --- /dev/null +++ b/modules/nf-core/modules/multiqc/functions.nf @@ -0,0 +1,68 @@ +// +// Utility functions used in nf-core DSL2 module files +// + +// +// Extract name of software tool from process name using $task.process +// +def getSoftwareName(task_process) { + return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() +} + +// +// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules +// +def initOptions(Map args) { + def Map options = [:] + options.args = args.args ?: '' + options.args2 = args.args2 ?: '' + options.args3 = args.args3 ?: '' + options.publish_by_meta = args.publish_by_meta ?: [] + options.publish_dir = args.publish_dir ?: '' + options.publish_files = args.publish_files + options.suffix = args.suffix ?: '' + return options +} + +// +// Tidy up and join elements of a list to return a path string +// +def getPathFromList(path_list) { + def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries + paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes + return paths.join('/') +} + +// +// Function to save/publish module results +// +def saveFiles(Map args) { + if (!args.filename.endsWith('.version.txt')) { + def ioptions = initOptions(args.options) + def path_list = [ ioptions.publish_dir ?: args.publish_dir ] + if (ioptions.publish_by_meta) { + def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta + for (key in key_list) { + if (args.meta && key instanceof String) { + def path = key + if (args.meta.containsKey(key)) { + path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] + } + path = path instanceof String ? path : '' + path_list.add(path) + } + } + } + if (ioptions.publish_files instanceof Map) { + for (ext in ioptions.publish_files) { + if (args.filename.endsWith(ext.key)) { + def ext_list = path_list.collect() + ext_list.add(ext.value) + return "${getPathFromList(ext_list)}/$args.filename" + } + } + } else if (ioptions.publish_files == null) { + return "${getPathFromList(path_list)}/$args.filename" + } + } +} diff --git a/modules/nf-core/modules/multiqc/main.nf b/modules/nf-core/modules/multiqc/main.nf new file mode 100644 index 00000000..da780800 --- /dev/null +++ b/modules/nf-core/modules/multiqc/main.nf @@ -0,0 +1,35 @@ +// Import generic module functions +include { initOptions; saveFiles; getSoftwareName } from './functions' + +params.options = [:] +options = initOptions(params.options) + +process MULTIQC { + label 'process_medium' + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:[:], publish_by_meta:[]) } + + conda (params.enable_conda ? "bioconda::multiqc=1.10.1" : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://depot.galaxyproject.org/singularity/multiqc:1.10.1--py_0" + } else { + container "quay.io/biocontainers/multiqc:1.10.1--py_0" + } + + input: + path multiqc_files + + output: + path "*multiqc_report.html", emit: report + path "*_data" , emit: data + path "*_plots" , optional:true, emit: plots + path "*.version.txt" , emit: version + + script: + def software = getSoftwareName(task.process) + """ + multiqc -f $options.args . + multiqc --version | sed -e "s/multiqc, version //g" > ${software}.version.txt + """ +} diff --git a/modules/nf-core/modules/multiqc/meta.yml b/modules/nf-core/modules/multiqc/meta.yml new file mode 100644 index 00000000..532a8bb1 --- /dev/null +++ b/modules/nf-core/modules/multiqc/meta.yml @@ -0,0 +1,39 @@ +name: MultiQC +description: Aggregate results from bioinformatics analyses across many samples into a single report +keywords: + - QC + - bioinformatics tools + - Beautiful stand-alone HTML report +tools: + - multiqc: + description: | + MultiQC searches a given directory for analysis logs and compiles a HTML report. + It's a general use tool, perfect for summarising the output from numerous bioinformatics tools. + homepage: https://multiqc.info/ + documentation: https://multiqc.info/docs/ +input: + - multiqc_files: + type: file + description: | + List of reports / files recognised by MultiQC, for example the html and zip output of FastQC +output: + - report: + type: file + description: MultiQC report file + pattern: "multiqc_report.html" + - data: + type: dir + description: MultiQC data dir + pattern: "multiqc_data" + - plots: + type: file + description: Plots created by MultiQC + pattern: "*_data" + - version: + type: file + description: File containing software version + pattern: "*.{version.txt}" +authors: + - "@abhi18av" + - "@bunop" + - "@drpatelh" diff --git a/nextflow.config b/nextflow.config index d3d97210..d925a1a1 100644 --- a/nextflow.config +++ b/nextflow.config @@ -1,130 +1,136 @@ /* - * ------------------------------------------------- - * nf-core/bacass Nextflow config file - * ------------------------------------------------- - * Default config options for all environments. - */ +======================================================================================== + nf-core/bacass Nextflow config file +======================================================================================== + Default config options for all compute environments +---------------------------------------------------------------------------------------- +*/ // Global default params, used in configs params { - // Workflow flags - // TODO nf-core: Specify your pipeline's command line flags - genome = false - input = null - input_paths = null - single_end = false - outdir = './results' - publish_dir_mode = 'copy' - - // Boilerplate options - multiqc_config = false - email = false - email_on_fail = false - max_multiqc_email_size = 25.MB - plaintext_email = false - monochrome_logs = false - help = false - igenomes_base = 's3://ngi-igenomes/igenomes' - tracedir = "${params.outdir}/pipeline_info" - igenomes_ignore = false - custom_config_version = 'master' - custom_config_base = "https://raw.githubusercontent.com/nf-core/configs/${params.custom_config_version}" - hostnames = false - config_profile_name = null - config_profile_description = false - config_profile_contact = false - config_profile_url = false - validate_params = true - show_hidden_params = false - schema_ignore_params = 'genomes,input_paths' - - // Defaults only, expecting to be overwritten - max_memory = 128.GB - max_cpus = 16 - max_time = 240.h + // TODO nf-core: Specify your pipeline's command line flags + // Input options + input = null -} + // References + genome = null + igenomes_base = 's3://ngi-igenomes/igenomes' + igenomes_ignore = false + + // MultiQC options + multiqc_config = null + multiqc_title = null + max_multiqc_email_size = '25.MB' + + // Boilerplate options + outdir = './results' + tracedir = "${params.outdir}/pipeline_info" + publish_dir_mode = 'copy' + email = null + email_on_fail = null + plaintext_email = false + monochrome_logs = false + help = false + validate_params = true + show_hidden_params = false + schema_ignore_params = 'genomes,modules' + enable_conda = false + singularity_pull_docker_container = false -// Container slug. Stable releases should specify release tag! -// Developmental code should specify :dev -process.container = 'nfcore/bacass:dev' + // Config options + custom_config_version = 'master' + custom_config_base = "https://raw.githubusercontent.com/nf-core/configs/${params.custom_config_version}" + hostnames = [:] + config_profile_description = null + config_profile_contact = null + config_profile_url = null + config_profile_name = null + + // Max resource options + // Defaults only, expecting to be overwritten + max_memory = '128.GB' + max_cpus = 16 + max_time = '240.h' + +} // Load base.config by default for all pipelines includeConfig 'conf/base.config' +// Load modules.config for DSL2 module specific options +includeConfig 'conf/modules.config' + // Load nf-core custom profiles from different Institutions try { - includeConfig "${params.custom_config_base}/nfcore_custom.config" + includeConfig "${params.custom_config_base}/nfcore_custom.config" } catch (Exception e) { - System.err.println("WARNING: Could not load nf-core/config profiles: ${params.custom_config_base}/nfcore_custom.config") -} - -profiles { - conda { - docker.enabled = false - singularity.enabled = false - podman.enabled = false - shifter.enabled = false - charliecloud.enabled = false - process.conda = "$projectDir/environment.yml" - } - debug { process.beforeScript = 'echo $HOSTNAME' } - docker { - docker.enabled = true - singularity.enabled = false - podman.enabled = false - shifter.enabled = false - charliecloud.enabled = false - // Avoid this error: - // WARNING: Your kernel does not support swap limit capabilities or the cgroup is not mounted. Memory limited without swap. - // Testing this in nf-core after discussion here https://github.com/nf-core/tools/pull/351 - // once this is established and works well, nextflow might implement this behavior as new default. - docker.runOptions = '-u \$(id -u):\$(id -g)' - } - singularity { - docker.enabled = false - singularity.enabled = true - podman.enabled = false - shifter.enabled = false - charliecloud.enabled = false - singularity.autoMounts = true - } - podman { - singularity.enabled = false - docker.enabled = false - podman.enabled = true - shifter.enabled = false - charliecloud.enabled = false - } - shifter { - singularity.enabled = false - docker.enabled = false - podman.enabled = false - shifter.enabled = true - charliecloud.enabled = false - } - charliecloud { - singularity.enabled = false - docker.enabled = false - podman.enabled = false - shifter.enabled = false - charliecloud.enabled = true - } - test { includeConfig 'conf/test.config' } - test_full { includeConfig 'conf/test_full.config' } + System.err.println("WARNING: Could not load nf-core/config profiles: ${params.custom_config_base}/nfcore_custom.config") } // Load igenomes.config if required if (!params.igenomes_ignore) { - includeConfig 'conf/igenomes.config' + includeConfig 'conf/igenomes.config' +} else { + params.genomes = [:] +} + +profiles { + debug { process.beforeScript = 'echo $HOSTNAME' } + conda { + params.enable_conda = true + docker.enabled = false + singularity.enabled = false + podman.enabled = false + shifter.enabled = false + charliecloud.enabled = false + } + docker { + docker.enabled = true + docker.userEmulation = true + singularity.enabled = false + podman.enabled = false + shifter.enabled = false + charliecloud.enabled = false + } + singularity { + singularity.enabled = true + singularity.autoMounts = true + docker.enabled = false + podman.enabled = false + shifter.enabled = false + charliecloud.enabled = false + } + podman { + podman.enabled = true + docker.enabled = false + singularity.enabled = false + shifter.enabled = false + charliecloud.enabled = false + } + shifter { + shifter.enabled = true + docker.enabled = false + singularity.enabled = false + podman.enabled = false + charliecloud.enabled = false + } + charliecloud { + charliecloud.enabled = true + docker.enabled = false + singularity.enabled = false + podman.enabled = false + shifter.enabled = false + } + test { includeConfig 'conf/test.config' } + test_full { includeConfig 'conf/test_full.config' } } // Export these variables to prevent local Python/R libraries from conflicting with those in the container env { - PYTHONNOUSERSITE = 1 - R_PROFILE_USER = "/.Rprofile" - R_ENVIRON_USER = "/.Renviron" + PYTHONNOUSERSITE = 1 + R_PROFILE_USER = "/.Rprofile" + R_ENVIRON_USER = "/.Renviron" } // Capture exit codes from upstream processes when piping @@ -132,61 +138,61 @@ process.shell = ['/bin/bash', '-euo', 'pipefail'] def trace_timestamp = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss') timeline { - enabled = true - file = "${params.tracedir}/execution_timeline_${trace_timestamp}.html" + enabled = true + file = "${params.tracedir}/execution_timeline_${trace_timestamp}.html" } report { - enabled = true - file = "${params.tracedir}/execution_report_${trace_timestamp}.html" + enabled = true + file = "${params.tracedir}/execution_report_${trace_timestamp}.html" } trace { - enabled = true - file = "${params.tracedir}/execution_trace_${trace_timestamp}.txt" + enabled = true + file = "${params.tracedir}/execution_trace_${trace_timestamp}.txt" } dag { - enabled = true - file = "${params.tracedir}/pipeline_dag_${trace_timestamp}.svg" + enabled = true + file = "${params.tracedir}/pipeline_dag_${trace_timestamp}.svg" } manifest { - name = 'nf-core/bacass' - author = 'Andreas Wilm, Alexander Peltzer' - homePage = 'https://github.com/nf-core/bacass' - description = 'Simple bacterial assembly and annotation pipeline.' - mainScript = 'main.nf' - nextflowVersion = '>=20.04.0' - version = '1.1.1' + name = 'nf-core/bacass' + author = 'Andreas Wilm, Alexander Peltzer' + homePage = 'https://github.com/nf-core/bacass' + description = 'Simple bacterial assembly and annotation pipeline.' + mainScript = 'main.nf' + nextflowVersion = '!>=21.04.0' + version = '1.1.1' } // Function to ensure that resource requirements don't go beyond // a maximum limit def check_max(obj, type) { - if (type == 'memory') { - try { - if (obj.compareTo(params.max_memory as nextflow.util.MemoryUnit) == 1) - return params.max_memory as nextflow.util.MemoryUnit - else - return obj - } catch (all) { - println " ### ERROR ### Max memory '${params.max_memory}' is not valid! Using default value: $obj" - return obj - } - } else if (type == 'time') { - try { - if (obj.compareTo(params.max_time as nextflow.util.Duration) == 1) - return params.max_time as nextflow.util.Duration - else - return obj - } catch (all) { - println " ### ERROR ### Max time '${params.max_time}' is not valid! Using default value: $obj" - return obj - } - } else if (type == 'cpus') { - try { - return Math.min( obj, params.max_cpus as int ) - } catch (all) { - println " ### ERROR ### Max cpus '${params.max_cpus}' is not valid! Using default value: $obj" - return obj + if (type == 'memory') { + try { + if (obj.compareTo(params.max_memory as nextflow.util.MemoryUnit) == 1) + return params.max_memory as nextflow.util.MemoryUnit + else + return obj + } catch (all) { + println " ### ERROR ### Max memory '${params.max_memory}' is not valid! Using default value: $obj" + return obj + } + } else if (type == 'time') { + try { + if (obj.compareTo(params.max_time as nextflow.util.Duration) == 1) + return params.max_time as nextflow.util.Duration + else + return obj + } catch (all) { + println " ### ERROR ### Max time '${params.max_time}' is not valid! Using default value: $obj" + return obj + } + } else if (type == 'cpus') { + try { + return Math.min( obj, params.max_cpus as int ) + } catch (all) { + println " ### ERROR ### Max cpus '${params.max_cpus}' is not valid! Using default value: $obj" + return obj + } } - } } diff --git a/nextflow_schema.json b/nextflow_schema.json index 6b345737..a69a9b41 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -16,19 +16,17 @@ "properties": { "input": { "type": "string", - "fa_icon": "fas fa-dna", - "description": "Input FastQ files.", - "help_text": "Use this to specify the location of your input FastQ files. For example:\n\n```bash\n--input 'path/to/data/sample_*_{1,2}.fastq'\n```\n\nPlease note the following requirements:\n\n1. The path must be enclosed in quotes\n2. The path must have at least one `*` wildcard character\n3. When using the pipeline with paired end data, the path must use `{1,2}` notation to specify read pairs.\n\nIf left unspecified, a default pattern is used: `data/*{1,2}.fastq.gz`" - }, - "single_end": { - "type": "boolean", - "description": "Specifies that the input is single-end reads.", - "fa_icon": "fas fa-align-center", - "help_text": "By default, the pipeline expects paired-end data. If you have single-end data, you need to specify `--single_end` on the command line when you launch the pipeline. A normal glob pattern, enclosed in quotation marks, can then be used for `--input`. For example:\n\n```bash\n--single_end --input '*.fastq'\n```\n\nIt is not possible to run a mixture of single-end and paired-end files in one run." + "format": "file-path", + "mimetype": "text/csv", + "pattern": "\\.csv$", + "schema": "assets/schema_input.json", + "description": "Path to comma-separated file containing information about the samples in the experiment.", + "help_text": "You will need to create a design file with information about the samples in your experiment before running the pipeline. Use this parameter to specify its location. It has to be a comma-separated file with 3 columns, and a header row. See [usage docs](https://nf-co.re/bacass/usage#samplesheet-input).", + "fa_icon": "fas fa-file-csv" }, "outdir": { "type": "string", - "description": "The output directory where the results will be saved.", + "description": "Path to the output directory where the results will be saved.", "default": "./results", "fa_icon": "fas fa-folder-open" }, @@ -38,6 +36,11 @@ "fa_icon": "fas fa-envelope", "help_text": "Set this parameter to your e-mail address to get a summary e-mail with details of the run sent to you when the workflow exits. If set in your user config file (`~/.nextflow/config`) then you don't need to specify this on the command line for every run.", "pattern": "^([a-zA-Z0-9_\\-\\.]+)@([a-zA-Z0-9_\\-\\.]+)\\.([a-zA-Z]{2,5})$" + }, + "multiqc_title": { + "type": "string", + "description": "MultiQC report title. Printed as page header, used for filename if not otherwise specified.", + "fa_icon": "fas fa-file-signature" } } }, @@ -45,22 +48,26 @@ "title": "Reference genome options", "type": "object", "fa_icon": "fas fa-dna", - "description": "Options for the reference genome indices used to align reads.", + "description": "Reference genome related files and options required for the workflow.", "properties": { "genome": { "type": "string", "description": "Name of iGenomes reference.", "fa_icon": "fas fa-book", - "help_text": "If using a reference genome configured in the pipeline using iGenomes, use this parameter to give the ID for the reference. This is then used to build the full paths for all required reference genome files e.g. `--genome GRCh38`.\n\nSee the [nf-core website docs](https://nf-co.re/usage/reference_genomes) for more details." + "help_text": "If using a reference genome configured in the pipeline using iGenomes, use this parameter to give the ID for the reference. This is then used to build the full paths for all required reference genome files e.g. `--genome GRCh38`. \n\nSee the [nf-core website docs](https://nf-co.re/usage/reference_genomes) for more details." }, "fasta": { "type": "string", - "fa_icon": "fas fa-font", + "format": "file-path", + "mimetype": "text/plain", + "pattern": "\\.fn?a(sta)?(\\.gz)?$", "description": "Path to FASTA genome file.", - "help_text": "If you have no genome reference available, the pipeline can build one using a FASTA file. This requires additional time and resources, so it's better to use a pre-build index if possible." + "help_text": "This parameter is *mandatory* if `--genome` is not specified. If you don't have a BWA index available this will be generated for you automatically. Combine with `--save_reference` to save BWA index for future runs.", + "fa_icon": "far fa-file-code" }, "igenomes_base": { "type": "string", + "format": "directory-path", "description": "Directory / URL base for iGenomes references.", "default": "s3://ngi-igenomes/igenomes", "fa_icon": "fas fa-cloud-download-alt", @@ -75,91 +82,57 @@ } } }, - "generic_options": { - "title": "Generic options", + "institutional_config_options": { + "title": "Institutional config options", "type": "object", - "fa_icon": "fas fa-file-import", - "description": "Less common options for the pipeline, typically set in a config file.", - "help_text": "These options are common to all nf-core pipelines and allow you to customise some of the core preferences for how the pipeline runs.\n\nTypically these options would be set in a Nextflow config file loaded for all pipeline runs, such as `~/.nextflow/config`.", + "fa_icon": "fas fa-university", + "description": "Parameters used to describe centralised config profiles. These should not be edited.", + "help_text": "The centralised nf-core configuration profiles use a handful of pipeline parameters to describe themselves. This information is then printed to the Nextflow log when you run a pipeline. You should not need to change these values when you run a pipeline.", "properties": { - "help": { - "type": "boolean", - "description": "Display help text.", - "hidden": true, - "fa_icon": "fas fa-question-circle" - }, - "publish_dir_mode": { + "custom_config_version": { "type": "string", - "default": "copy", + "description": "Git commit id for Institutional configs.", + "default": "master", "hidden": true, - "description": "Method used to save pipeline results to output directory.", - "help_text": "The Nextflow `publishDir` option specifies which intermediate files should be saved to the output directory. This option tells the pipeline what method should be used to move these files. See [Nextflow docs](https://www.nextflow.io/docs/latest/process.html#publishdir) for details.", - "fa_icon": "fas fa-copy", - "enum": [ - "symlink", - "rellink", - "link", - "copy", - "copyNoFollow", - "move" - ] - }, - "validate_params": { - "type": "boolean", - "description": "Boolean whether to validate parameters against the schema at runtime", - "default": true, - "fa_icon": "fas fa-check-square", - "hidden": true + "fa_icon": "fas fa-users-cog" }, - "email_on_fail": { + "custom_config_base": { "type": "string", - "description": "Email address for completion summary, only when pipeline fails.", - "fa_icon": "fas fa-exclamation-triangle", - "pattern": "^([a-zA-Z0-9_\\-\\.]+)@([a-zA-Z0-9_\\-\\.]+)\\.([a-zA-Z]{2,5})$", + "description": "Base directory for Institutional configs.", + "default": "https://raw.githubusercontent.com/nf-core/configs/master", "hidden": true, - "help_text": "This works exactly as with `--email`, except emails are only sent if the workflow is not successful." + "help_text": "If you're running offline, Nextflow will not be able to fetch the institutional config files from the internet. If you don't need them, then this is not a problem. If you do need them, you should download the files from the repo and tell Nextflow where to find them with this parameter.", + "fa_icon": "fas fa-users-cog" }, - "plaintext_email": { - "type": "boolean", - "description": "Send plain-text email instead of HTML.", - "fa_icon": "fas fa-remove-format", + "hostnames": { + "type": "string", + "description": "Institutional configs hostname.", "hidden": true, - "help_text": "Set to receive plain-text e-mails instead of HTML formatted." + "fa_icon": "fas fa-users-cog" }, - "max_multiqc_email_size": { + "config_profile_name": { "type": "string", - "description": "File size limit when attaching MultiQC reports to summary emails.", - "default": "25.MB", - "fa_icon": "fas fa-file-upload", + "description": "Institutional config name.", "hidden": true, - "help_text": "If file generated by pipeline exceeds the threshold, it will not be attached." + "fa_icon": "fas fa-users-cog" }, - "monochrome_logs": { - "type": "boolean", - "description": "Do not use coloured log outputs.", - "fa_icon": "fas fa-palette", + "config_profile_description": { + "type": "string", + "description": "Institutional config description.", "hidden": true, - "help_text": "Set to disable colourful command line output and live life in monochrome." + "fa_icon": "fas fa-users-cog" }, - "multiqc_config": { + "config_profile_contact": { "type": "string", - "description": "Custom config file to supply to MultiQC.", - "fa_icon": "fas fa-cog", - "hidden": true + "description": "Institutional config contact information.", + "hidden": true, + "fa_icon": "fas fa-users-cog" }, - "tracedir": { + "config_profile_url": { "type": "string", - "description": "Directory to keep pipeline Nextflow logs and reports.", - "default": "${params.outdir}/pipeline_info", - "fa_icon": "fas fa-cogs", - "hidden": true - }, - "show_hidden_params": { - "type": "boolean", - "fa_icon": "far fa-eye-slash", - "description": "Show all params when using `--help`", + "description": "Institutional config URL link.", "hidden": true, - "help_text": "By default, parameters set as _hidden_ in the schema are not shown on the command line when a user runs with `--help`. Specifying this option will tell the pipeline to show all parameters." + "fa_icon": "fas fa-users-cog" } } }, @@ -172,7 +145,7 @@ "properties": { "max_cpus": { "type": "integer", - "description": "Maximum number of CPUs that can be requested for any single job.", + "description": "Maximum number of CPUs that can be requested for any single job.", "default": 16, "fa_icon": "fas fa-microchip", "hidden": true, @@ -198,58 +171,102 @@ } } }, - "institutional_config_options": { - "title": "Institutional config options", + "generic_options": { + "title": "Generic options", "type": "object", - "fa_icon": "fas fa-university", - "description": "Parameters used to describe centralised config profiles. These should not be edited.", - "help_text": "The centralised nf-core configuration profiles use a handful of pipeline parameters to describe themselves. This information is then printed to the Nextflow log when you run a pipeline. You should not need to change these values when you run a pipeline.", + "fa_icon": "fas fa-file-import", + "description": "Less common options for the pipeline, typically set in a config file.", + "help_text": "These options are common to all nf-core pipelines and allow you to customise some of the core preferences for how the pipeline runs.\n\nTypically these options would be set in a Nextflow config file loaded for all pipeline runs, such as `~/.nextflow/config`.", "properties": { - "custom_config_version": { + "help": { + "type": "boolean", + "description": "Display help text.", + "fa_icon": "fas fa-question-circle", + "hidden": true + }, + "publish_dir_mode": { "type": "string", - "description": "Git commit id for Institutional configs.", - "default": "master", - "hidden": true, - "fa_icon": "fas fa-users-cog", - "help_text": "Provide git commit id for custom Institutional configs hosted at `nf-core/configs`. This was implemented for reproducibility purposes. Default: `master`.\n\n```bash\n## Download and use config file with following git commit id\n--custom_config_version d52db660777c4bf36546ddb188ec530c3ada1b96\n```" + "default": "copy", + "description": "Method used to save pipeline results to output directory.", + "help_text": "The Nextflow `publishDir` option specifies which intermediate files should be saved to the output directory. This option tells the pipeline what method should be used to move these files. See [Nextflow docs](https://www.nextflow.io/docs/latest/process.html#publishdir) for details.", + "fa_icon": "fas fa-copy", + "enum": [ + "symlink", + "rellink", + "link", + "copy", + "copyNoFollow", + "move" + ], + "hidden": true }, - "custom_config_base": { + "email_on_fail": { "type": "string", - "description": "Base directory for Institutional configs.", - "default": "https://raw.githubusercontent.com/nf-core/configs/master", - "hidden": true, - "help_text": "If you're running offline, nextflow will not be able to fetch the institutional config files from the internet. If you don't need them, then this is not a problem. If you do need them, you should download the files from the repo and tell nextflow where to find them with the `custom_config_base` option. For example:\n\n```bash\n## Download and unzip the config files\ncd /path/to/my/configs\nwget https://github.com/nf-core/configs/archive/master.zip\nunzip master.zip\n\n## Run the pipeline\ncd /path/to/my/data\nnextflow run /path/to/pipeline/ --custom_config_base /path/to/my/configs/configs-master/\n```\n\n> Note that the nf-core/tools helper package has a `download` command to download all required pipeline files + singularity containers + institutional configs in one go for you, to make this process easier.", - "fa_icon": "fas fa-users-cog" + "description": "Email address for completion summary, only when pipeline fails.", + "fa_icon": "fas fa-exclamation-triangle", + "pattern": "^([a-zA-Z0-9_\\-\\.]+)@([a-zA-Z0-9_\\-\\.]+)\\.([a-zA-Z]{2,5})$", + "help_text": "An email address to send a summary email to when the pipeline is completed - ONLY sent if the pipeline does not exit successfully.", + "hidden": true }, - "hostnames": { + "plaintext_email": { + "type": "boolean", + "description": "Send plain-text email instead of HTML.", + "fa_icon": "fas fa-remove-format", + "hidden": true + }, + "max_multiqc_email_size": { "type": "string", - "description": "Institutional configs hostname.", - "hidden": true, - "fa_icon": "fas fa-users-cog" + "description": "File size limit when attaching MultiQC reports to summary emails.", + "pattern": "^\\d+(\\.\\d+)?\\.?\\s*(K|M|G|T)?B$", + "default": "25.MB", + "fa_icon": "fas fa-file-upload", + "hidden": true }, - "config_profile_name": { + "monochrome_logs": { + "type": "boolean", + "description": "Do not use coloured log outputs.", + "fa_icon": "fas fa-palette", + "hidden": true + }, + "multiqc_config": { "type": "string", - "description": "Institutional config name.", - "hidden": true, - "fa_icon": "fas fa-users-cog" + "description": "Custom config file to supply to MultiQC.", + "fa_icon": "fas fa-cog", + "hidden": true }, - "config_profile_description": { + "tracedir": { "type": "string", - "description": "Institutional config description.", + "description": "Directory to keep pipeline Nextflow logs and reports.", + "default": "${params.outdir}/pipeline_info", + "fa_icon": "fas fa-cogs", + "hidden": true + }, + "validate_params": { + "type": "boolean", + "description": "Boolean whether to validate parameters against the schema at runtime", + "default": true, + "fa_icon": "fas fa-check-square", + "hidden": true + }, + "show_hidden_params": { + "type": "boolean", + "fa_icon": "far fa-eye-slash", + "description": "Show all params when using `--help`", "hidden": true, - "fa_icon": "fas fa-users-cog" + "help_text": "By default, parameters set as _hidden_ in the schema are not shown on the command line when a user runs with `--help`. Specifying this option will tell the pipeline to show all parameters." }, - "config_profile_contact": { - "type": "string", - "description": "Institutional config contact information.", + "enable_conda": { + "type": "boolean", + "description": "Run this workflow with Conda. You can also use '-profile conda' instead of providing this parameter.", "hidden": true, - "fa_icon": "fas fa-users-cog" + "fa_icon": "fas fa-bacon" }, - "config_profile_url": { - "type": "string", - "description": "Institutional config URL link.", + "singularity_pull_docker_container": { + "type": "boolean", + "description": "Instead of directly downloading Singularity images for use with Singularity, force the workflow to pull and convert Docker containers instead.", "hidden": true, - "fa_icon": "fas fa-users-cog" + "fa_icon": "fas fa-toolbox", + "help_text": "This may be useful for example if you are unable to directly pull Singularity containers to run the pipeline due to http/https proxy issues." } } } @@ -262,13 +279,13 @@ "$ref": "#/definitions/reference_genome_options" }, { - "$ref": "#/definitions/generic_options" + "$ref": "#/definitions/institutional_config_options" }, { "$ref": "#/definitions/max_job_request_options" }, { - "$ref": "#/definitions/institutional_config_options" + "$ref": "#/definitions/generic_options" } ] } diff --git a/subworkflows/local/input_check.nf b/subworkflows/local/input_check.nf new file mode 100644 index 00000000..b664bc8c --- /dev/null +++ b/subworkflows/local/input_check.nf @@ -0,0 +1,42 @@ +// +// Check input samplesheet and get read channels +// + +params.options = [:] + +include { SAMPLESHEET_CHECK } from '../../modules/local/samplesheet_check' addParams( options: params.options ) + +workflow INPUT_CHECK { + take: + samplesheet // file: /path/to/samplesheet.csv + + main: + SAMPLESHEET_CHECK ( samplesheet ) + .splitCsv ( header:true, sep:',' ) + .map { create_fastq_channels(it) } + .set { reads } + + emit: + reads // channel: [ val(meta), [ reads ] ] +} + +// Function to get list of [ meta, [ fastq_1, fastq_2 ] ] +def create_fastq_channels(LinkedHashMap row) { + def meta = [:] + meta.id = row.sample + meta.single_end = row.single_end.toBoolean() + + def array = [] + if (!file(row.fastq_1).exists()) { + exit 1, "ERROR: Please check input samplesheet -> Read 1 FastQ file does not exist!\n${row.fastq_1}" + } + if (meta.single_end) { + array = [ meta, [ file(row.fastq_1) ] ] + } else { + if (!file(row.fastq_2).exists()) { + exit 1, "ERROR: Please check input samplesheet -> Read 2 FastQ file does not exist!\n${row.fastq_2}" + } + array = [ meta, [ file(row.fastq_1), file(row.fastq_2) ] ] + } + return array +} diff --git a/workflows/bacass.nf b/workflows/bacass.nf new file mode 100644 index 00000000..a6d9db3f --- /dev/null +++ b/workflows/bacass.nf @@ -0,0 +1,141 @@ +/* +======================================================================================== + VALIDATE INPUTS +======================================================================================== +*/ + +def summary_params = NfcoreSchema.paramsSummaryMap(workflow, params) + +// Validate input parameters +WorkflowBacass.initialise(params, log) + +// TODO nf-core: Add all file path parameters for the pipeline to the list below +// Check input path parameters to see if they exist +def checkPathParamList = [ params.input, params.multiqc_config, params.fasta ] +for (param in checkPathParamList) { if (param) { file(param, checkIfExists: true) } } + +// Check mandatory parameters +if (params.input) { ch_input = file(params.input) } else { exit 1, 'Input samplesheet not specified!' } + +/* +======================================================================================== + CONFIG FILES +======================================================================================== +*/ + +ch_multiqc_config = file("$projectDir/assets/multiqc_config.yaml", checkIfExists: true) +ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath(params.multiqc_config) : Channel.empty() + +/* +======================================================================================== + IMPORT LOCAL MODULES/SUBWORKFLOWS +======================================================================================== +*/ + +// Don't overwrite global params.modules, create a copy instead and use that within the main script. +def modules = params.modules.clone() + +// +// MODULE: Local to the pipeline +// +include { GET_SOFTWARE_VERSIONS } from '../modules/local/get_software_versions' addParams( options: [publish_files : ['tsv':'']] ) + +// +// SUBWORKFLOW: Consisting of a mix of local and nf-core/modules +// +include { INPUT_CHECK } from '../subworkflows/local/input_check' addParams( options: [:] ) + +/* +======================================================================================== + IMPORT NF-CORE MODULES/SUBWORKFLOWS +======================================================================================== +*/ + +def multiqc_options = modules['multiqc'] +multiqc_options.args += params.multiqc_title ? Utils.joinModuleArgs(["--title \"$params.multiqc_title\""]) : '' + +// +// MODULE: Installed directly from nf-core/modules +// +include { FASTQC } from '../modules/nf-core/modules/fastqc/main' addParams( options: modules['fastqc'] ) +include { MULTIQC } from '../modules/nf-core/modules/multiqc/main' addParams( options: multiqc_options ) + +/* +======================================================================================== + RUN MAIN WORKFLOW +======================================================================================== +*/ + +// Info required for completion email and summary +def multiqc_report = [] + +workflow BACASS { + + ch_software_versions = Channel.empty() + + // + // SUBWORKFLOW: Read in samplesheet, validate and stage input files + // + INPUT_CHECK ( + ch_input + ) + + // + // MODULE: Run FastQC + // + FASTQC ( + INPUT_CHECK.out.reads + ) + ch_software_versions = ch_software_versions.mix(FASTQC.out.version.first().ifEmpty(null)) + + // + // MODULE: Pipeline reporting + // + ch_software_versions + .map { it -> if (it) [ it.baseName, it ] } + .groupTuple() + .map { it[1][0] } + .flatten() + .collect() + .set { ch_software_versions } + + GET_SOFTWARE_VERSIONS ( + ch_software_versions.map { it }.collect() + ) + + // + // MODULE: MultiQC + // + workflow_summary = WorkflowBacass.paramsSummaryMultiqc(workflow, summary_params) + ch_workflow_summary = Channel.value(workflow_summary) + + ch_multiqc_files = Channel.empty() + ch_multiqc_files = ch_multiqc_files.mix(Channel.from(ch_multiqc_config)) + ch_multiqc_files = ch_multiqc_files.mix(ch_multiqc_custom_config.collect().ifEmpty([])) + ch_multiqc_files = ch_multiqc_files.mix(ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml')) + ch_multiqc_files = ch_multiqc_files.mix(GET_SOFTWARE_VERSIONS.out.yaml.collect()) + ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]}.ifEmpty([])) + + MULTIQC ( + ch_multiqc_files.collect() + ) + multiqc_report = MULTIQC.out.report.toList() + ch_software_versions = ch_software_versions.mix(MULTIQC.out.version.ifEmpty(null)) +} + +/* +======================================================================================== + COMPLETION EMAIL AND SUMMARY +======================================================================================== +*/ + +workflow.onComplete { + NfcoreTemplate.email(workflow, params, summary_params, projectDir, log, multiqc_report) + NfcoreTemplate.summary(workflow, params, log) +} + +/* +======================================================================================== + THE END +======================================================================================== +*/ From db29c73fbd0c3c88256d512f7a8587cb31511f65 Mon Sep 17 00:00:00 2001 From: nf-core-bot Date: Tue, 13 Jul 2021 15:24:46 +0000 Subject: [PATCH 11/81] Template update for nf-core/tools version 2.0.1 --- .github/workflows/linting.yml | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml index 13b4fc81..fbde5cf8 100644 --- a/.github/workflows/linting.yml +++ b/.github/workflows/linting.yml @@ -127,7 +127,7 @@ jobs: GITHUB_COMMENTS_URL: ${{ github.event.pull_request.comments_url }} GITHUB_TOKEN: ${{ secrets.GITHUB_TOKEN }} GITHUB_PR_COMMIT: ${{ github.event.pull_request.head.sha }} - run: nf-core -l lint_log.txt lint ${GITHUB_WORKSPACE} --markdown lint_results.md + run: nf-core -l lint_log.txt lint --dir ${GITHUB_WORKSPACE} --markdown lint_results.md - name: Save PR number if: ${{ always() }} From e19ae659e924a628f013b2f1d88041f052d44994 Mon Sep 17 00:00:00 2001 From: nf-core-bot Date: Tue, 27 Jul 2021 15:10:40 +0000 Subject: [PATCH 12/81] Template update for nf-core/tools version 2.1 --- .editorconfig | 3 +++ .github/CONTRIBUTING.md | 6 +++--- .github/PULL_REQUEST_TEMPLATE.md | 2 +- .github/workflows/linting.yml | 2 +- README.md | 2 +- bin/scrape_software_versions.py | 2 +- lib/NfcoreTemplate.groovy | 22 +++++++++++++--------- nextflow_schema.json | 4 ++-- workflows/bacass.nf | 4 +++- 9 files changed, 28 insertions(+), 19 deletions(-) diff --git a/.editorconfig b/.editorconfig index afb20bb1..95549501 100644 --- a/.editorconfig +++ b/.editorconfig @@ -11,6 +11,9 @@ indent_style = space [*.{yml,yaml}] indent_size = 2 +[*.json] +insert_final_newline = unset + # These files are edited and tested upstream in nf-core/modules [/modules/nf-core/**] charset = unset diff --git a/.github/CONTRIBUTING.md b/.github/CONTRIBUTING.md index af1c3c5b..42b647d0 100644 --- a/.github/CONTRIBUTING.md +++ b/.github/CONTRIBUTING.md @@ -19,7 +19,7 @@ If you'd like to write some code for nf-core/bacass, the standard workflow is as * If there isn't one already, please create one so that others know you're working on this 2. [Fork](https://help.github.com/en/github/getting-started-with-github/fork-a-repo) the [nf-core/bacass repository](https://github.com/nf-core/bacass) to your GitHub account 3. Make the necessary changes / additions within your forked repository following [Pipeline conventions](#pipeline-contribution-conventions) -4. Use `nf-core schema build .` and add any new parameters to the pipeline JSON schema (requires [nf-core tools](https://github.com/nf-core/tools) >= 1.10). +4. Use `nf-core schema build` and add any new parameters to the pipeline JSON schema (requires [nf-core tools](https://github.com/nf-core/tools) >= 1.10). 5. Submit a Pull Request against the `dev` branch and wait for the code to be reviewed and merged If you're not used to this workflow with git, you can start with some [docs from GitHub](https://help.github.com/en/github/collaborating-with-issues-and-pull-requests) or even their [excellent `git` resources](https://try.github.io/). @@ -69,7 +69,7 @@ If you wish to contribute a new step, please use the following coding standards: 2. Write the process block (see below). 3. Define the output channel if needed (see below). 4. Add any new flags/options to `nextflow.config` with a default (see below). -5. Add any new flags/options to `nextflow_schema.json` with help text (with `nf-core schema build .`). +5. Add any new flags/options to `nextflow_schema.json` with help text (with `nf-core schema build`). 6. Add any new flags/options to the help message (for integer/text parameters, print to help the corresponding `nextflow.config` parameter). 7. Add sanity checks for all relevant parameters. 8. Add any new software to the `scrape_software_versions.py` script in `bin/` and the version command to the `scrape_software_versions` process in `main.nf`. @@ -83,7 +83,7 @@ If you wish to contribute a new step, please use the following coding standards: Parameters should be initialised / defined with default values in `nextflow.config` under the `params` scope. -Once there, use `nf-core schema build .` to add to `nextflow_schema.json`. +Once there, use `nf-core schema build` to add to `nextflow_schema.json`. ### Default processes resource requirements diff --git a/.github/PULL_REQUEST_TEMPLATE.md b/.github/PULL_REQUEST_TEMPLATE.md index dc75e978..dcccebd8 100644 --- a/.github/PULL_REQUEST_TEMPLATE.md +++ b/.github/PULL_REQUEST_TEMPLATE.md @@ -18,7 +18,7 @@ Learn more about contributing: [CONTRIBUTING.md](https://github.com/nf-core/baca - [ ] If you've fixed a bug or added code that should be tested, add tests! - [ ] If you've added a new tool - have you followed the pipeline conventions in the [contribution docs](https://github.com/nf-core/bacass/tree/master/.github/CONTRIBUTING.md) - [ ] If necessary, also make a PR on the nf-core/bacass _branch_ on the [nf-core/test-datasets](https://github.com/nf-core/test-datasets) repository. -- [ ] Make sure your code lints (`nf-core lint .`). +- [ ] Make sure your code lints (`nf-core lint`). - [ ] Ensure the test suite passes (`nextflow run . -profile test,docker`). - [ ] Usage Documentation in `docs/usage.md` is updated. - [ ] Output Documentation in `docs/output.md` is updated. diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml index fbde5cf8..3b448773 100644 --- a/.github/workflows/linting.yml +++ b/.github/workflows/linting.yml @@ -53,7 +53,7 @@ jobs: - uses: actions/setup-node@v1 with: - node-version: "10" + node-version: '10' - name: Install editorconfig-checker run: npm install -g editorconfig-checker diff --git a/README.md b/README.md index 53353e7f..cbdd61c0 100644 --- a/README.md +++ b/README.md @@ -33,7 +33,7 @@ On release, automated continuous integration tests run the pipeline on a full-si ## Quick Start -1. Install [`Nextflow`](https://nf-co.re/usage/installation) (`>=21.04.0`) +1. Install [`Nextflow`](https://www.nextflow.io/docs/latest/getstarted.html#installation) (`>=21.04.0`) 2. Install any of [`Docker`](https://docs.docker.com/engine/installation/), [`Singularity`](https://www.sylabs.io/guides/3.0/user-guide/), [`Podman`](https://podman.io/), [`Shifter`](https://nersc.gitlab.io/development/shifter/how-to-use/) or [`Charliecloud`](https://hpc.github.io/charliecloud/) for full pipeline reproducibility _(please only use [`Conda`](https://conda.io/miniconda.html) as a last resort; see [docs](https://nf-co.re/usage/configuration#basic-configuration-profiles))_ diff --git a/bin/scrape_software_versions.py b/bin/scrape_software_versions.py index b4dc6516..df04fa4a 100755 --- a/bin/scrape_software_versions.py +++ b/bin/scrape_software_versions.py @@ -30,7 +30,7 @@ print("
{}
{}
".format(k, v)) print(" ") -# Write out regexes as csv file: +# Write out as tsv file: with open("software_versions.tsv", "w") as f: for k, v in sorted(results.items()): f.write("{}\t{}\n".format(k, v)) diff --git a/lib/NfcoreTemplate.groovy b/lib/NfcoreTemplate.groovy index b6e689ec..44551e0a 100755 --- a/lib/NfcoreTemplate.groovy +++ b/lib/NfcoreTemplate.groovy @@ -24,17 +24,21 @@ class NfcoreTemplate { public static void hostName(workflow, params, log) { Map colors = logColours(params.monochrome_logs) if (params.hostnames) { - def hostname = "hostname".execute().text.trim() - params.hostnames.each { prof, hnames -> - hnames.each { hname -> - if (hostname.contains(hname) && !workflow.profile.contains(prof)) { - log.info "=${colors.yellow}====================================================${colors.reset}=\n" + - "${colors.yellow}WARN: You are running with `-profile $workflow.profile`\n" + - " but your machine hostname is ${colors.white}'$hostname'${colors.reset}.\n" + - " ${colors.yellow_bold}Please use `-profile $prof${colors.reset}`\n" + - "=${colors.yellow}====================================================${colors.reset}=" + try { + def hostname = "hostname".execute().text.trim() + params.hostnames.each { prof, hnames -> + hnames.each { hname -> + if (hostname.contains(hname) && !workflow.profile.contains(prof)) { + log.info "=${colors.yellow}====================================================${colors.reset}=\n" + + "${colors.yellow}WARN: You are running with `-profile $workflow.profile`\n" + + " but your machine hostname is ${colors.white}'$hostname'${colors.reset}.\n" + + " ${colors.yellow_bold}Please use `-profile $prof${colors.reset}`\n" + + "=${colors.yellow}====================================================${colors.reset}=" + } } } + } catch (Exception e) { + log.warn "[$workflow.manifest.name] Could not determine 'hostname' - skipping check. Reason: ${e.message}." } } } diff --git a/nextflow_schema.json b/nextflow_schema.json index a69a9b41..240d7591 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -18,7 +18,7 @@ "type": "string", "format": "file-path", "mimetype": "text/csv", - "pattern": "\\.csv$", + "pattern": "^\\S+\\.csv$", "schema": "assets/schema_input.json", "description": "Path to comma-separated file containing information about the samples in the experiment.", "help_text": "You will need to create a design file with information about the samples in your experiment before running the pipeline. Use this parameter to specify its location. It has to be a comma-separated file with 3 columns, and a header row. See [usage docs](https://nf-co.re/bacass/usage#samplesheet-input).", @@ -60,7 +60,7 @@ "type": "string", "format": "file-path", "mimetype": "text/plain", - "pattern": "\\.fn?a(sta)?(\\.gz)?$", + "pattern": "^\\S+\\.fn?a(sta)?(\\.gz)?$", "description": "Path to FASTA genome file.", "help_text": "This parameter is *mandatory* if `--genome` is not specified. If you don't have a BWA index available this will be generated for you automatically. Combine with `--save_reference` to save BWA index for future runs.", "fa_icon": "far fa-file-code" diff --git a/workflows/bacass.nf b/workflows/bacass.nf index a6d9db3f..b00c86a5 100644 --- a/workflows/bacass.nf +++ b/workflows/bacass.nf @@ -130,7 +130,9 @@ workflow BACASS { */ workflow.onComplete { - NfcoreTemplate.email(workflow, params, summary_params, projectDir, log, multiqc_report) + if (params.email || params.email_on_fail) { + NfcoreTemplate.email(workflow, params, summary_params, projectDir, log, multiqc_report) + } NfcoreTemplate.summary(workflow, params, log) } From e578b4fda60aa1499ecf796ab136648e2ad3c563 Mon Sep 17 00:00:00 2001 From: d4straub Date: Mon, 2 Aug 2021 16:45:26 +0200 Subject: [PATCH 13/81] fix CHANGELOG.md --- CHANGELOG.md | 38 +++++++++++++++++++------------------- 1 file changed, 19 insertions(+), 19 deletions(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 5c90a9c4..ffbf505a 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -21,32 +21,32 @@ This is basically a maintenance update that includes template updates, fixed env * Merged in nf-core/tools template v 1.10.2 * Updated dependencies - * fastqc=0.11.8, 0.11.9 - * multiqc=1.8, 1.9 - * kraken2=2.0.8_beta, 2.0.9beta - * prokka=1.14.5, 1.14.6 - * nanopolish=0.11.2, 0.13.2 - * parallel=20191122, 20200922 - * racon=1.4.10, 1.4.13 - * canu=1.9, 2.0 - * samtools=1.9, 1.11 - * nanoplot=1.28.1, 1.32.1 - * pycoqc=2.5.0.3, 2.5.0.23 + * fastqc=0.11.8, 0.11.9 + * multiqc=1.8, 1.9 + * kraken2=2.0.8_beta, 2.0.9beta + * prokka=1.14.5, 1.14.6 + * nanopolish=0.11.2, 0.13.2 + * parallel=20191122, 20200922 + * racon=1.4.10, 1.4.13 + * canu=1.9, 2.0 + * samtools=1.9, 1.11 + * nanoplot=1.28.1, 1.32.1 + * pycoqc=2.5.0.3, 2.5.0.23 * Switched out containers for many tools to make DSLv2 transition easier (escape from dependency hell) ## v1.1.0 nf-core/bacass: "Green Aluminium Shark" 2019/12/13 * Added support for hybrid assembly using Nanopore and Illumina Short Reads * Added methods for long-read Nanopore data - * Nanopolish, for polishing of Nanopore data with Illumina reads - * Medaka, as alternative assembly polishing method - * PoreChop, for quality trimming of Nanopore data - * Nanoplot, for plotting quality metrics of Nanopore data - * PycoQC, to QC Nanopore data + * Nanopolish, for polishing of Nanopore data with Illumina reads + * Medaka, as alternative assembly polishing method + * PoreChop, for quality trimming of Nanopore data + * Nanoplot, for plotting quality metrics of Nanopore data + * PycoQC, to QC Nanopore data * Added multiple tools to assemble long-reads - * Miniasm + Racon - * Canu Assembler - * Unicycler in Long read Mode + * Miniasm + Racon + * Canu Assembler + * Unicycler in Long read Mode * Add alternative assembly annotation using DFAST * Add social preview image From 239a48f442d4647bea7b8a77dfca2252685cc03a Mon Sep 17 00:00:00 2001 From: d4straub Date: Mon, 2 Aug 2021 16:46:36 +0200 Subject: [PATCH 14/81] fix linting --- assets/nf-core-bacass_logo.png 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z`+ai{bQf1Cm@`50_UwCFy@BFTYBrq}ad# zhYmO*^KvMa{-G6QXJ(gP)Ze7Kerr;mYpS3E{KM`4{`>zg11BymC Date: Mon, 2 Aug 2021 17:11:06 +0200 Subject: [PATCH 15/81] update description and README --- README.md | 22 +++++++++++------ assets/email_template.html | 2 +- modules/local/abc.nf | 50 ++++++++++++++++++++++++++++++++++++++ nextflow.config | 2 +- nextflow_schema.json | 2 +- 5 files changed, 67 insertions(+), 11 deletions(-) create mode 100644 modules/local/abc.nf diff --git a/README.md b/README.md index cbdd61c0..574aacc5 100644 --- a/README.md +++ b/README.md @@ -3,7 +3,7 @@ [![GitHub Actions CI Status](https://github.com/nf-core/bacass/workflows/nf-core%20CI/badge.svg)](https://github.com/nf-core/bacass/actions?query=workflow%3A%22nf-core+CI%22) [![GitHub Actions Linting Status](https://github.com/nf-core/bacass/workflows/nf-core%20linting/badge.svg)](https://github.com/nf-core/bacass/actions?query=workflow%3A%22nf-core+linting%22) [![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/bacass/results) -[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX) +[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.2669428-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.2669428) [![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A521.04.0-23aa62.svg?labelColor=000000)](https://www.nextflow.io/) [![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/) @@ -16,8 +16,7 @@ ## Introduction - -**nf-core/bacass** is a bioinformatics best-practice analysis pipeline for Simple bacterial assembly and annotation pipeline.. +**nf-core/bacass** is a bioinformatics best-practice analysis pipeline for simple bacterial assembly and annotation. The pipeline is able to assemble short reads, long reads, or a mixture of short and long reads (hybrid assembly). The pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool to run tasks across multiple compute infrastructures in a very portable manner. It uses Docker/Singularity containers making installation trivial and results highly reproducible. The [Nextflow DSL2](https://www.nextflow.io/docs/latest/dsl2.html) implementation of this pipeline uses one container per process which makes it much easier to maintain and update software dependencies. Where possible, these processes have been submitted to and installed from [nf-core/modules](https://github.com/nf-core/modules) in order to make them available to all nf-core pipelines, and to everyone within the Nextflow community! @@ -26,10 +25,18 @@ On release, automated continuous integration tests run the pipeline on a full-si ## Pipeline summary - +### Short Read Assembly -1. Read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/)) -2. Present QC for raw reads ([`MultiQC`](http://multiqc.info/)) +This pipeline is primarily for bacterial assembly of next-generation sequencing reads. It can be used to quality trim your reads using [Skewer](https://github.com/relipmoc/skewer) and performs basic sequencing QC using [FastQC](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/). Afterwards, the pipeline performs read assembly using [Unicycler](https://github.com/rrwick/Unicycler). Contamination of the assembly is checked using [Kraken2](https://ccb.jhu.edu/software/kraken2/) to verify sample purity. + +### Long Read Assembly + +For users that only have Nanopore data, the pipeline quality trims these using [PoreChop](https://github.com/rrwick/Porechop) and assesses basic sequencing QC utilizing [NanoPlot](https://github.com/wdecoster/NanoPlot) and [PycoQC](https://github.com/a-slide/pycoQC). +The pipeline can then perform long read assembly utilizing [Unicycler](https://github.com/rrwick/Unicycler), [Miniasm](https://github.com/lh3/miniasm) in combination with [Racon](https://github.com/isovic/racon) or [Canu](https://github.com/marbl/canu). Long reads can be polished using specified Fast5 files with [NanoPolish](https://github.com/jts/nanopolish). + +### Hybrid Assembly + +For users specifying both short read and long read (NanoPore) data, the pipeline can perform a hybrid assembly approach utilizing [Unicycler](https://github.com/rrwick/Unicycler), taking the full set of information from short reads and long reads into account. ## Quick Start @@ -75,8 +82,7 @@ For further information or help, don't hesitate to get in touch on the [Slack `# ## Citations - - +If you use nf-core/bacass for your analysis, please cite it using the following doi: [10.5281/zenodo.2669428](https://doi.org/10.5281/zenodo.2669428) An extensive list of references for the tools used by the pipeline can be found in the [`CITATIONS.md`](CITATIONS.md) file. diff --git a/assets/email_template.html b/assets/email_template.html index 332ae36b..ebf8e7a3 100644 --- a/assets/email_template.html +++ b/assets/email_template.html @@ -4,7 +4,7 @@ - + nf-core/bacass Pipeline Report diff --git a/modules/local/abc.nf b/modules/local/abc.nf new file mode 100644 index 00000000..194a1aab --- /dev/null +++ b/modules/local/abc.nf @@ -0,0 +1,50 @@ +// Import generic module functions +include { initOptions; saveFiles; getSoftwareName } from './functions' + +params.options = [:] +options = initOptions(params.options) + +process DADA2_FILTNTRIM { + tag "$meta.id" + label 'process_medium' + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), publish_id:'') } + + conda (params.enable_conda ? "bioconductor-dada2=1.18.0" : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://depot.galaxyproject.org/singularity/bioconductor-dada2:1.18.0--r40h5f743cb_0" + } else { + container "quay.io/biocontainers/bioconductor-dada2:1.18.0--r40h5f743cb_0" + } + + input: + tuple val(meta), path(reads), val(trunclenf), val(trunclenr) + + output: + tuple val(meta), path("*.filter_stats.tsv"), emit: log + tuple val(meta), path("*.filt.fastq.gz") , emit: reads + path "*.version.txt" , emit: version + path "*.args.txt" , emit: args + + script: + def software = getSoftwareName(task.process) + def in_and_out = meta.single_end ? "\"${reads}\", \"${meta.id}.filt.fastq.gz\"" : "\"${reads[0]}\", \"${meta.id}_1.filt.fastq.gz\", \"${reads[1]}\", \"${meta.id}_2.filt.fastq.gz\"" + def trunclenf = trunclenf[1].toInteger() + def trunclenr = trunclenr[1].toInteger() + def trunc_args = meta.single_end ? "truncLen = $trunclenf" : "truncLen = c($trunclenf, $trunclenr)" + """ + #!/usr/bin/env Rscript + suppressPackageStartupMessages(library(dada2)) + out <- filterAndTrim($in_and_out, + $trunc_args, + $options.args, + compress = TRUE, + multithread = $task.cpus, + verbose = TRUE) + out <- cbind(out, ID = row.names(out)) + write.table( out, file = "${meta.id}.filter_stats.tsv", sep = "\t", row.names = FALSE, quote = FALSE) + write.table(paste('filterAndTrim\t$trunc_args','$options.args',sep=","), file = "filterAndTrim.args.txt", row.names = FALSE, col.names = FALSE, quote = FALSE) + write.table(packageVersion("dada2"), file = "${software}.version.txt", row.names = FALSE, col.names = FALSE, quote = FALSE) + """ +} diff --git a/nextflow.config b/nextflow.config index d925a1a1..dba81d2f 100644 --- a/nextflow.config +++ b/nextflow.config @@ -158,7 +158,7 @@ manifest { name = 'nf-core/bacass' author = 'Andreas Wilm, Alexander Peltzer' homePage = 'https://github.com/nf-core/bacass' - description = 'Simple bacterial assembly and annotation pipeline.' + description = 'Simple bacterial assembly and annotation' mainScript = 'main.nf' nextflowVersion = '!>=21.04.0' version = '1.1.1' diff --git a/nextflow_schema.json b/nextflow_schema.json index 240d7591..b7ebdc00 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -2,7 +2,7 @@ "$schema": "http://json-schema.org/draft-07/schema", "$id": "https://raw.githubusercontent.com/nf-core/bacass/master/nextflow_schema.json", "title": "nf-core/bacass pipeline parameters", - "description": "Simple bacterial assembly and annotation pipeline.", + "description": "Simple bacterial assembly and annotation", "type": "object", "definitions": { "input_output_options": { From b6d1a8ff28cebaee66df38272b29667f9f8fec86 Mon Sep 17 00:00:00 2001 From: d4straub Date: Tue, 3 Aug 2021 13:22:56 +0200 Subject: [PATCH 16/81] update schema and version --- nextflow.config | 28 ++++++++- nextflow_schema.json | 136 ++++++++++++++++++++++++++++++++++++++++++- 2 files changed, 159 insertions(+), 5 deletions(-) diff --git a/nextflow.config b/nextflow.config index dba81d2f..1af3273c 100644 --- a/nextflow.config +++ b/nextflow.config @@ -9,10 +9,32 @@ // Global default params, used in configs params { - // TODO nf-core: Specify your pipeline's command line flags // Input options input = null + // Contamination_screening + kraken2db = "" + + // Assembly parameters + assembler = 'unicycler' //allowed are unicycler, canu, miniasm + assembly_type = 'short' //allowed are short, long, hybrid (hybrid works only with Unicycler) + unicycler_args = "" + canu_args = '' //Default no extra options, can be adjusted by the user + + // Assembly polishing + polish_method = 'medaka' + + // Annotation + annotation_tool = 'prokka' + prokka_args = "" + dfast_config = "$baseDir/assets/test_config_dfast.py" + + // Skipping options + skip_kraken2 = false + skip_pycoqc = false + skip_annotation = false + skip_polish = false + // References genome = null igenomes_base = 's3://ngi-igenomes/igenomes' @@ -161,7 +183,7 @@ manifest { description = 'Simple bacterial assembly and annotation' mainScript = 'main.nf' nextflowVersion = '!>=21.04.0' - version = '1.1.1' + version = '2.0.0dev' } // Function to ensure that resource requirements don't go beyond @@ -195,4 +217,4 @@ def check_max(obj, type) { return obj } } -} +} \ No newline at end of file diff --git a/nextflow_schema.json b/nextflow_schema.json index b7ebdc00..3f0d976d 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -21,7 +21,7 @@ "pattern": "^\\S+\\.csv$", "schema": "assets/schema_input.json", "description": "Path to comma-separated file containing information about the samples in the experiment.", - "help_text": "You will need to create a design file with information about the samples in your experiment before running the pipeline. Use this parameter to specify its location. It has to be a comma-separated file with 3 columns, and a header row. See [usage docs](https://nf-co.re/bacass/usage#samplesheet-input).", + "help_text": "You will need to create a design file with information about the samples in your experiment before running the pipeline. Use this parameter to specify its location. It has to be a comma-separated file with 6 columns, and a header row. See [usage docs](https://nf-co.re/bacass/usage#samplesheet-input).\n\nFor example:\n\n`--input 'design_hybrid.csv'`\n\nAn example of properly formatted input files can be found at the [nf-core/testData](https://github.com/nf-core/test-datasets/tree/bacass). \n\nExemplarily, this is the input used for a hybrid assembly in testing:\nID,R1,R2,LongFastQ,Fast5,GenomeSize\nERR044595,ERR044595_1M_1.fastq.gz,ERR044595_1M_2.fastq.gz,subset15000.fq.gz,NA,2.8m\n\n* `ID`: The identifier to use for handling the dataset e.g. sample name\n* `R1`: The forward reads in case of available short-read data\n* `R2`: The reverse reads in case of available short-read data\n* `LongFastQ`: The long read FastQ file with reads in FASTQ format\n* `Fast5`: The folder containing the basecalled FAST5 files\n* `GenomeSize`: The expected genome size of the assembly. Only used by the canu assembler.\n\nMissing values (e.g. FAST5 folder in case of short reads) can be omitted by using a `NA` in the CSV file. The pipeline will handle such cases appropriately then.", "fa_icon": "fas fa-file-csv" }, "outdir": { @@ -44,6 +44,123 @@ } } }, + "contamination_screening": { + "title": "Contamination Screening", + "type": "object", + "description": "", + "default": "", + "fa_icon": "fas fa-box", + "properties": { + "kraken2db": { + "type": "string", + "fa_icon": "fab fa-gitkraken", + "help_text": "See [Kraken2 homepage](https://benlangmead.github.io/aws-indexes/k2) for download\nlinks. Minikraken2 8GB is a reasonable choice, since we run Kraken here mainly just to check for\nsample purity.", + "description": "Path to Kraken2 database." + } + } + }, + "assembly_parameters": { + "title": "Assembly parameters", + "type": "object", + "description": "Parameters for the assembly", + "default": "", + "fa_icon": "fas fa-puzzle-piece", + "properties": { + "assembler": { + "type": "string", + "default": "unicycler", + "fa_icon": "fas fa-puzzle-piece", + "description": "The assembler to use for assembly. Available options are `Unicycler`, `Canu`, `Miniasm`. The latter two are only available for long-read data, whereas Unicycler can be used for short or hybrid assembly projects." + }, + "assembly_type": { + "type": "string", + "default": "short", + "fa_icon": "fas fa-fingerprint", + "help_text": "This adjusts the type of assembly done with the input data and can be any of `long`, `short` or `hybrid`. Short & Hybrid assembly will always run Unicycler, whereas long-read assembly can be configured separately using the `--assembler` parameter.", + "description": "Which type of assembly to perform." + }, + "unicycler_args": { + "type": "string", + "fa_icon": "fas fa-bicycle", + "description": "Extra arguments for Unicycler", + "help_text": "This advanced option allows you to pass extra arguments to Unicycler (e.g. `\"--mode conservative\"` or `\"--no_correct\"`). For this to work you need to quote the arguments and add at least one space." + }, + "canu_args": { + "type": "string", + "fa_icon": "fas fa-ship", + "description": "This can be used to supply [extra options](https://canu.readthedocs.io/en/latest/quick-start.html) to the Canu assembler. Will be ignored when other assemblers are used." + } + } + }, + "assembly_polishing": { + "title": "Assembly Polishing", + "type": "object", + "description": "", + "default": "", + "fa_icon": "fas fa-user-astronaut", + "properties": { + "polish_method": { + "type": "string", + "default": "medaka", + "fa_icon": "fas fa-hotdog", + "description": "Which assembly polishing method to use.", + "help_text": "Can be used to define which polishing method is used by default for long reads. Default is `medaka`, available options are `nanopolish` or `medaka`." + } + } + }, + "annotation": { + "title": "Annotation", + "type": "object", + "description": "", + "default": "", + "fa_icon": "fas fa-align-left", + "properties": { + "annotation_tool": { + "type": "string", + "default": "prokka", + "description": "The annotation method to annotate the final assembly. Default choice is `prokka`, but the `dfast` tool is also available. For the latter, make sure to create your specific config if you're not happy with the default one provided. See [#dfast_config](#dfastconfig) to find out how." + }, + "prokka_args": { + "type": "string", + "description": "Extra arguments for prokka annotation tool.", + "help_text": "This advanced option allows you to pass extra arguments to Prokka (e.g. `\" --rfam\"` or `\" --genus name\"`). For this to work you need to quote the arguments and add at least one space between the arguments. Example:\n\n```bash\n--prokka_args `--rfam --genus Escherichia Coli`\n```\n" + }, + "dfast_config": { + "type": "string", + "default": "/Users/alexanderpeltzer/IDEA/nf-core/bacass/assets/test_config_dfast.py", + "description": "Specifies a configuration file for the [DFAST](https://github.com/nigyta/dfast_core) annotation method. This can be used instead of PROKKA if required to specify a specific config file for annotation. If you want to know how to create your config file, please refer to the [DFAST](https://github.com/nigyta/dfast_core) readme on how to create one. > The default config is just included for testing, so if you want to annotate using DFAST, you have to create a config!" + } + } + }, + "skipping_options": { + "title": "Skipping Options", + "type": "object", + "description": "", + "default": "", + "fa_icon": "fas fa-forward", + "properties": { + "skip_kraken2": { + "type": "string", + "fa_icon": "fas fa-forward", + "description": "Skip running Kraken2 classifier on reads." + }, + "skip_annotation": { + "type": "string", + "fa_icon": "fas fa-forward", + "description": "Skip annotating the assembly with Prokka /DFAST." + }, + "skip_pycoqc": { + "type": "string", + "fa_icon": "fas fa-forward", + "description": "Skip running `PycoQC` on long read input." + }, + "skip_polish": { + "type": "string", + "fa_icon": "fas fa-forward", + "description": "Skip polishing the long-read assembly with FAST5 input. Will not affect short/hybrid assemblies." + } + } + }, "reference_genome_options": { "title": "Reference genome options", "type": "object", @@ -275,6 +392,21 @@ { "$ref": "#/definitions/input_output_options" }, + { + "$ref": "#/definitions/contamination_screening" + }, + { + "$ref": "#/definitions/assembly_parameters" + }, + { + "$ref": "#/definitions/assembly_polishing" + }, + { + "$ref": "#/definitions/annotation" + }, + { + "$ref": "#/definitions/skipping_options" + }, { "$ref": "#/definitions/reference_genome_options" }, @@ -288,4 +420,4 @@ "$ref": "#/definitions/generic_options" } ] -} +} \ No newline at end of file From 5afb220ff574a2432cb0c9f0b2ad52950aeaa3e9 Mon Sep 17 00:00:00 2001 From: d4straub Date: Tue, 3 Aug 2021 14:53:53 +0200 Subject: [PATCH 17/81] change samplesheet to csv and fix parsing --- .github/workflows/ci.yml | 1 - bin/check_samplesheet.py | 17 +++++++------- lib/WorkflowBacass.groovy | 4 ++-- nextflow_schema.json | 12 +++++----- subworkflows/local/input_check.nf | 39 +++++++++++++++++++++++++++---- workflows/bacass.nf | 2 +- 6 files changed, 52 insertions(+), 23 deletions(-) diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index bcbfc32c..7f5d08e6 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -36,7 +36,6 @@ jobs: sudo mv nextflow /usr/local/bin/ - name: Run pipeline with test data - # TODO nf-core: You can customise CI pipeline run tests as required # For example: adding multiple test runs with different parameters # Remember that you can parallelise this by using strategy.matrix run: | diff --git a/bin/check_samplesheet.py b/bin/check_samplesheet.py index cef3e4fe..310bdf0e 100755 --- a/bin/check_samplesheet.py +++ b/bin/check_samplesheet.py @@ -43,12 +43,12 @@ def check_samplesheet(file_in, file_out): """ This function checks that the samplesheet follows the following structure: - sample,fastq_1,fastq_2 - SAMPLE_PE,SAMPLE_PE_RUN1_1.fastq.gz,SAMPLE_PE_RUN1_2.fastq.gz - SAMPLE_PE,SAMPLE_PE_RUN2_1.fastq.gz,SAMPLE_PE_RUN2_2.fastq.gz - SAMPLE_SE,SAMPLE_SE_RUN1_1.fastq.gz, + sample,fastq_1,fastq_2,long_fastq,fast5,genome_size + SAMPLE_PE,SAMPLE_PE_1.fastq.gz,SAMPLE_PE_2.fastq.gz,SAMPLE_LONG.fastq.gz,SAMPLE_LONG.fast5,2.8m + SAMPLE_PE,SAMPLE_PE_1.fastq.gz,SAMPLE_PE_2.fastq.gz,NA,NA,2.8m For an example see: + TODO: link actual example https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv """ @@ -56,9 +56,8 @@ def check_samplesheet(file_in, file_out): with open(file_in, "r") as fin: ## Check header - MIN_COLS = 2 - # TODO nf-core: Update the column names for the input samplesheet - HEADER = ["sample", "fastq_1", "fastq_2"] + MIN_COLS = 6 + HEADER = ["sample", "fastq_1", "fastq_2", "long_fastq", "fast5", "genome_size"] header = [x.strip('"') for x in fin.readline().strip().split(",")] if header[: len(HEADER)] != HEADER: print("ERROR: Please check samplesheet header -> {} != {}".format(",".join(header), ",".join(HEADER))) @@ -84,7 +83,7 @@ def check_samplesheet(file_in, file_out): ) ## Check sample name entries - sample, fastq_1, fastq_2 = lspl[: len(HEADER)] + sample, fastq_1, fastq_2, long_fastq, fast5, genome_size = lspl[: len(HEADER)] sample = sample.replace(" ", "_") if not sample: print_error("Sample entry has not been specified!", "Line", line) @@ -124,7 +123,7 @@ def check_samplesheet(file_in, file_out): out_dir = os.path.dirname(file_out) make_dir(out_dir) with open(file_out, "w") as fout: - fout.write(",".join(["sample", "single_end", "fastq_1", "fastq_2"]) + "\n") + fout.write(",".join(["sample", "single_end", "fastq_1", "fastq_2", "long_fastq", "fast5", "genome_size"]) + "\n") for sample in sorted(sample_mapping_dict.keys()): ## Check that multiple runs of the same sample are of the same datatype diff --git a/lib/WorkflowBacass.groovy b/lib/WorkflowBacass.groovy index 94f7e96d..79d7fba0 100755 --- a/lib/WorkflowBacass.groovy +++ b/lib/WorkflowBacass.groovy @@ -10,8 +10,8 @@ class WorkflowBacass { public static void initialise(params, log) { genomeExistsError(params, log) - if (!params.fasta) { - log.error "Genome fasta file not specified with e.g. '--fasta genome.fa' or via a detectable config file." + if(("${params.assembler}" == 'canu' || "${params.assembler}" == 'miniasm') && ("${params.assembly_type}" == 'short' || "${params.assembly_type}" == 'hybrid')){ + log.error "Canu and Miniasm can only be used for long read assembly and neither for Hybrid nor Shortread assembly!" System.exit(1) } } diff --git a/nextflow_schema.json b/nextflow_schema.json index 3f0d976d..71156fa6 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -21,7 +21,7 @@ "pattern": "^\\S+\\.csv$", "schema": "assets/schema_input.json", "description": "Path to comma-separated file containing information about the samples in the experiment.", - "help_text": "You will need to create a design file with information about the samples in your experiment before running the pipeline. Use this parameter to specify its location. It has to be a comma-separated file with 6 columns, and a header row. See [usage docs](https://nf-co.re/bacass/usage#samplesheet-input).\n\nFor example:\n\n`--input 'design_hybrid.csv'`\n\nAn example of properly formatted input files can be found at the [nf-core/testData](https://github.com/nf-core/test-datasets/tree/bacass). \n\nExemplarily, this is the input used for a hybrid assembly in testing:\nID,R1,R2,LongFastQ,Fast5,GenomeSize\nERR044595,ERR044595_1M_1.fastq.gz,ERR044595_1M_2.fastq.gz,subset15000.fq.gz,NA,2.8m\n\n* `ID`: The identifier to use for handling the dataset e.g. sample name\n* `R1`: The forward reads in case of available short-read data\n* `R2`: The reverse reads in case of available short-read data\n* `LongFastQ`: The long read FastQ file with reads in FASTQ format\n* `Fast5`: The folder containing the basecalled FAST5 files\n* `GenomeSize`: The expected genome size of the assembly. Only used by the canu assembler.\n\nMissing values (e.g. FAST5 folder in case of short reads) can be omitted by using a `NA` in the CSV file. The pipeline will handle such cases appropriately then.", + "help_text": "You will need to create a design file with information about the samples in your experiment before running the pipeline. Use this parameter to specify its location. It has to be a comma-separated file with 6 columns, and a header row. See [usage docs](https://nf-co.re/bacass/usage#samplesheet-input).\n\nFor example:\n\n`--input 'design_hybrid.csv'`\n\nAn example of properly formatted input files can be found at the [nf-core/testData](https://github.com/nf-core/test-datasets/tree/bacass). \n\nExemplarily, this is the input used for a hybrid assembly in testing:\nID,R1,R2,long_fastq,fast5,genome_size\nERR044595,ERR044595_1M_1.fastq.gz,ERR044595_1M_2.fastq.gz,subset15000.fq.gz,NA,2.8m\n\n* `ID`: The identifier to use for handling the dataset e.g. sample name\n* `R1`: The forward reads in case of available short-read data\n* `R2`: The reverse reads in case of available short-read data\n* `long_fastq`: The long read FastQ file with reads in FASTQ format\n* `fast5`: The folder containing the basecalled fast5 files\n* `genome_size`: The expected genome size of the assembly. Only used by the canu assembler.\n\nMissing values (e.g. fast5 folder in case of short reads) can be omitted by using a `NA` in the CSV file. The pipeline will handle such cases appropriately then.", "fa_icon": "fas fa-file-csv" }, "outdir": { @@ -140,24 +140,24 @@ "fa_icon": "fas fa-forward", "properties": { "skip_kraken2": { - "type": "string", + "type": "boolean", "fa_icon": "fas fa-forward", "description": "Skip running Kraken2 classifier on reads." }, "skip_annotation": { - "type": "string", + "type": "boolean", "fa_icon": "fas fa-forward", "description": "Skip annotating the assembly with Prokka /DFAST." }, "skip_pycoqc": { - "type": "string", + "type": "boolean", "fa_icon": "fas fa-forward", "description": "Skip running `PycoQC` on long read input." }, "skip_polish": { - "type": "string", + "type": "boolean", "fa_icon": "fas fa-forward", - "description": "Skip polishing the long-read assembly with FAST5 input. Will not affect short/hybrid assemblies." + "description": "Skip polishing the long-read assembly with fast5 input. Will not affect short/hybrid assemblies." } } }, diff --git a/subworkflows/local/input_check.nf b/subworkflows/local/input_check.nf index b664bc8c..07431af8 100644 --- a/subworkflows/local/input_check.nf +++ b/subworkflows/local/input_check.nf @@ -16,27 +16,58 @@ workflow INPUT_CHECK { .map { create_fastq_channels(it) } .set { reads } + // reconfigure channels + reads + .map { meta, reads, long_fastq, fast5 -> [ meta, reads ] } + .filter{ meta, reads -> reads != 'NA' } // TODO: filter when PE and 'NA' + .set { shortreads } + reads + .map { meta, reads, long_fastq, fast5 -> [ meta, long_fastq, fast5 ] } + .filter{ meta, long_fastq, fast5 -> long_fastq != 'NA' && fast5 != 'NA' } + .set { longreads } + emit: - reads // channel: [ val(meta), [ reads ] ] + reads // channel: [ val(meta), [ reads ], long_fastq, fast5 ] + shortreads // channel: [ val(meta), [ reads ] ] + longreads // channel: [ val(meta), long_fastq, fast5 ] } -// Function to get list of [ meta, [ fastq_1, fastq_2 ] ] +// Function to get list of [ meta, [ fastq_1, fastq_2 ], long_fastq, fast5 ] def create_fastq_channels(LinkedHashMap row) { def meta = [:] meta.id = row.sample meta.single_end = row.single_end.toBoolean() + meta.genome_size = row.genome_size == null ? 'NA' : row.genome_size def array = [] if (!file(row.fastq_1).exists()) { exit 1, "ERROR: Please check input samplesheet -> Read 1 FastQ file does not exist!\n${row.fastq_1}" } + + // check long_fastq + if (!row.long_fastq == 'NA') { + if (!file(row.long_fastq).exists()) { + exit 1, "ERROR: Please check input samplesheet -> Long FastQ file does not exist!\n${row.fastq_1}" + } + long_fastq = file(row.long_fastq) + } else { long_fastq = 'NA' } + + // check long_fastq + if (!row.fast5 == 'NA') { + if (!file(row.fast5).exists()) { + exit 1, "ERROR: Please check input samplesheet -> Fast5 file does not exist!\n${row.fastq_1}" + } + fast5 = file(row.fast5) + } else { fast5 = 'NA' } + + // prepare output if (meta.single_end) { - array = [ meta, [ file(row.fastq_1) ] ] + array = [ meta, [ file(row.fastq_1) ], long_fastq, fast5 ] } else { if (!file(row.fastq_2).exists()) { exit 1, "ERROR: Please check input samplesheet -> Read 2 FastQ file does not exist!\n${row.fastq_2}" } - array = [ meta, [ file(row.fastq_1), file(row.fastq_2) ] ] + array = [ meta, [ file(row.fastq_1), file(row.fastq_2) ], long_fastq, fast5 ] } return array } diff --git a/workflows/bacass.nf b/workflows/bacass.nf index b00c86a5..a154917b 100644 --- a/workflows/bacass.nf +++ b/workflows/bacass.nf @@ -84,7 +84,7 @@ workflow BACASS { // MODULE: Run FastQC // FASTQC ( - INPUT_CHECK.out.reads + INPUT_CHECK.out.shortreads ) ch_software_versions = ch_software_versions.mix(FASTQC.out.version.first().ifEmpty(null)) From 2cc2e70d9d4a5a8300f4e330f2457858f7ba5731 Mon Sep 17 00:00:00 2001 From: d4straub Date: Tue, 3 Aug 2021 15:00:09 +0200 Subject: [PATCH 18/81] fix linting --- nextflow.config | 2 +- subworkflows/local/input_check.nf | 2 +- 2 files changed, 2 insertions(+), 2 deletions(-) diff --git a/nextflow.config b/nextflow.config index 1af3273c..fb2d37fb 100644 --- a/nextflow.config +++ b/nextflow.config @@ -217,4 +217,4 @@ def check_max(obj, type) { return obj } } -} \ No newline at end of file +} diff --git a/subworkflows/local/input_check.nf b/subworkflows/local/input_check.nf index 07431af8..16f36459 100644 --- a/subworkflows/local/input_check.nf +++ b/subworkflows/local/input_check.nf @@ -29,7 +29,7 @@ workflow INPUT_CHECK { emit: reads // channel: [ val(meta), [ reads ], long_fastq, fast5 ] shortreads // channel: [ val(meta), [ reads ] ] - longreads // channel: [ val(meta), long_fastq, fast5 ] + longreads // channel: [ val(meta), long_fastq, fast5 ] } // Function to get list of [ meta, [ fastq_1, fastq_2 ], long_fastq, fast5 ] From 335e17556f4f3e57d961a841a2ad66e69302f7ab Mon Sep 17 00:00:00 2001 From: d4straub Date: Tue, 3 Aug 2021 17:18:09 +0200 Subject: [PATCH 19/81] add skewer --- conf/modules.config | 5 +++++ modules/local/abc.nf | 50 ----------------------------------------- modules/local/skewer.nf | 46 +++++++++++++++++++++++++++++++++++++ workflows/bacass.nf | 9 ++++++++ 4 files changed, 60 insertions(+), 50 deletions(-) delete mode 100644 modules/local/abc.nf create mode 100644 modules/local/skewer.nf diff --git a/conf/modules.config b/conf/modules.config index 0b1bfdec..f598aca3 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -25,6 +25,11 @@ params { 'fastqc' { args = "--quiet" } + 'skewer' { + args = "-m pe -q 3 -n --quiet" + publish_dir = "./trimming/shortreads/" + publish_by_meta = true + } 'multiqc' { args = "" } diff --git a/modules/local/abc.nf b/modules/local/abc.nf deleted file mode 100644 index 194a1aab..00000000 --- a/modules/local/abc.nf +++ /dev/null @@ -1,50 +0,0 @@ -// Import generic module functions -include { initOptions; saveFiles; getSoftwareName } from './functions' - -params.options = [:] -options = initOptions(params.options) - -process DADA2_FILTNTRIM { - tag "$meta.id" - label 'process_medium' - publishDir "${params.outdir}", - mode: params.publish_dir_mode, - saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), publish_id:'') } - - conda (params.enable_conda ? "bioconductor-dada2=1.18.0" : null) - if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://depot.galaxyproject.org/singularity/bioconductor-dada2:1.18.0--r40h5f743cb_0" - } else { - container "quay.io/biocontainers/bioconductor-dada2:1.18.0--r40h5f743cb_0" - } - - input: - tuple val(meta), path(reads), val(trunclenf), val(trunclenr) - - output: - tuple val(meta), path("*.filter_stats.tsv"), emit: log - tuple val(meta), path("*.filt.fastq.gz") , emit: reads - path "*.version.txt" , emit: version - path "*.args.txt" , emit: args - - script: - def software = getSoftwareName(task.process) - def in_and_out = meta.single_end ? "\"${reads}\", \"${meta.id}.filt.fastq.gz\"" : "\"${reads[0]}\", \"${meta.id}_1.filt.fastq.gz\", \"${reads[1]}\", \"${meta.id}_2.filt.fastq.gz\"" - def trunclenf = trunclenf[1].toInteger() - def trunclenr = trunclenr[1].toInteger() - def trunc_args = meta.single_end ? "truncLen = $trunclenf" : "truncLen = c($trunclenf, $trunclenr)" - """ - #!/usr/bin/env Rscript - suppressPackageStartupMessages(library(dada2)) - out <- filterAndTrim($in_and_out, - $trunc_args, - $options.args, - compress = TRUE, - multithread = $task.cpus, - verbose = TRUE) - out <- cbind(out, ID = row.names(out)) - write.table( out, file = "${meta.id}.filter_stats.tsv", sep = "\t", row.names = FALSE, quote = FALSE) - write.table(paste('filterAndTrim\t$trunc_args','$options.args',sep=","), file = "filterAndTrim.args.txt", row.names = FALSE, col.names = FALSE, quote = FALSE) - write.table(packageVersion("dada2"), file = "${software}.version.txt", row.names = FALSE, col.names = FALSE, quote = FALSE) - """ -} diff --git a/modules/local/skewer.nf b/modules/local/skewer.nf new file mode 100644 index 00000000..659502f5 --- /dev/null +++ b/modules/local/skewer.nf @@ -0,0 +1,46 @@ +// Import generic module functions +include { initOptions; saveFiles; getSoftwareName } from './functions' + +params.options = [:] +options = initOptions(params.options) + +process SKEWER { + tag "$meta.id" + label 'process_medium' + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), publish_id:'') } + + conda (params.enable_conda ? "skewer:0.2.2-3" : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://depot.galaxyproject.org/singularity/skewer:0.2.2--hc9558a2_3" + } else { + container "quay.io/biocontainers/skewer:0.2.2--hc9558a2_3" + } + + input: + tuple val(meta), path(reads) + + output: + tuple val(meta), path("*_trm-cmb.R{1,2}.fastq.gz") , emit: reads + path("*.log"), emit: log + //path "*.version.txt" , emit: version // TODO: output version! + + script: + def software = getSoftwareName(task.process) + """ + # loop over readunits in pairs per sample + pairno=0 + echo "${reads[0]} ${reads[1]}" | xargs -n2 | while read fq1 fq2; do + skewer $options.args -t ${task.cpus} \$fq1 \$fq2; + done + + # gzip, because skewer's -z returns an error + gzip *.fastq + + cat \$(ls *trimmed-pair1.fastq.gz | sort) >> ${meta.id}_trm-cmb.R1.fastq.gz + cat \$(ls *trimmed-pair2.fastq.gz | sort) >> ${meta.id}_trm-cmb.R2.fastq.gz + + #skewer --version + """ +} diff --git a/workflows/bacass.nf b/workflows/bacass.nf index a154917b..aab8ccfc 100644 --- a/workflows/bacass.nf +++ b/workflows/bacass.nf @@ -39,6 +39,7 @@ def modules = params.modules.clone() // MODULE: Local to the pipeline // include { GET_SOFTWARE_VERSIONS } from '../modules/local/get_software_versions' addParams( options: [publish_files : ['tsv':'']] ) +include { SKEWER } from '../modules/local/skewer' addParams( options: modules['skewer'] ) // // SUBWORKFLOW: Consisting of a mix of local and nf-core/modules @@ -88,6 +89,14 @@ workflow BACASS { ) ch_software_versions = ch_software_versions.mix(FASTQC.out.version.first().ifEmpty(null)) + // + // MODULE: Run Skewer, trim and combine short read read-pairs per sample. Similar to nf-core vipr + // + SKEWER ( + INPUT_CHECK.out.shortreads + ) + //ch_software_versions = ch_software_versions.mix(SKEWER.out.version.first().ifEmpty(null)) //TODO + // // MODULE: Pipeline reporting // From b513931395dccaa0c9b0788666c25b3618911f2b Mon Sep 17 00:00:00 2001 From: d4straub Date: Tue, 3 Aug 2021 17:19:51 +0200 Subject: [PATCH 20/81] fix linting --- modules/local/skewer.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/modules/local/skewer.nf b/modules/local/skewer.nf index 659502f5..3aa0bc72 100644 --- a/modules/local/skewer.nf +++ b/modules/local/skewer.nf @@ -32,7 +32,7 @@ process SKEWER { # loop over readunits in pairs per sample pairno=0 echo "${reads[0]} ${reads[1]}" | xargs -n2 | while read fq1 fq2; do - skewer $options.args -t ${task.cpus} \$fq1 \$fq2; + skewer $options.args -t ${task.cpus} \$fq1 \$fq2; done # gzip, because skewer's -z returns an error From c64afa097ffc08223bdc22bb63880a23138a0bba Mon Sep 17 00:00:00 2001 From: d4straub Date: Wed, 4 Aug 2021 17:11:20 +0200 Subject: [PATCH 21/81] add more modules --- conf/modules.config | 20 +++++ modules.json | 14 +++- modules/local/porechop.nf | 34 ++++++++ modules/local/skewer.nf | 2 +- modules/nf-core/modules/nanoplot/functions.nf | 68 +++++++++++++++ modules/nf-core/modules/nanoplot/main.nf | 42 ++++++++++ modules/nf-core/modules/nanoplot/meta.yml | 58 +++++++++++++ modules/nf-core/modules/pycoqc/functions.nf | 68 +++++++++++++++ modules/nf-core/modules/pycoqc/main.nf | 40 +++++++++ modules/nf-core/modules/pycoqc/meta.yml | 48 +++++++++++ modules/nf-core/modules/spades/functions.nf | 68 +++++++++++++++ modules/nf-core/modules/spades/main.nf | 67 +++++++++++++++ modules/nf-core/modules/spades/meta.yml | 69 +++++++++++++++ .../nf-core/modules/unicycler/functions.nf | 68 +++++++++++++++ modules/nf-core/modules/unicycler/main.nf | 47 +++++++++++ modules/nf-core/modules/unicycler/meta.yml | 55 ++++++++++++ subworkflows/local/input_check.nf | 11 ++- workflows/bacass.nf | 84 ++++++++++++++++++- 18 files changed, 854 insertions(+), 9 deletions(-) create mode 100644 modules/local/porechop.nf create mode 100644 modules/nf-core/modules/nanoplot/functions.nf create mode 100644 modules/nf-core/modules/nanoplot/main.nf create mode 100644 modules/nf-core/modules/nanoplot/meta.yml create mode 100644 modules/nf-core/modules/pycoqc/functions.nf create mode 100644 modules/nf-core/modules/pycoqc/main.nf create mode 100644 modules/nf-core/modules/pycoqc/meta.yml create mode 100644 modules/nf-core/modules/spades/functions.nf create mode 100644 modules/nf-core/modules/spades/main.nf create mode 100644 modules/nf-core/modules/spades/meta.yml create mode 100644 modules/nf-core/modules/unicycler/functions.nf create mode 100644 modules/nf-core/modules/unicycler/main.nf create mode 100644 modules/nf-core/modules/unicycler/meta.yml diff --git a/conf/modules.config b/conf/modules.config index f598aca3..14bb820f 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -30,6 +30,26 @@ params { publish_dir = "./trimming/shortreads/" publish_by_meta = true } + 'nanoplot' { + args = "" + publish_dir = "./QC_longreads/NanoPlot" + } + 'pycoqc' { + args = "" + publish_dir = "./QC_longreads/PycoQC" + } + 'porechop' { + args = "" + publish_dir = "./trimming/longreads/" + } + 'unicycler' { + args = "" + publish_dir = "./Unicycler" + } + 'spades' { + args = "" + publish_dir = "./SPAdes" + } 'multiqc' { args = "" } diff --git a/modules.json b/modules.json index 7d548cca..051ac69c 100644 --- a/modules.json +++ b/modules.json @@ -8,7 +8,19 @@ }, "multiqc": { "git_sha": "e937c7950af70930d1f34bb961403d9d2aa81c7d" + }, + "nanoplot": { + "git_sha": "c5235a983d454787fa0c3247b02086969217163b" + }, + "pycoqc": { + "git_sha": "e937c7950af70930d1f34bb961403d9d2aa81c7d" + }, + "spades": { + "git_sha": "c5235a983d454787fa0c3247b02086969217163b" + }, + "unicycler": { + "git_sha": "e937c7950af70930d1f34bb961403d9d2aa81c7d" } } } -} +} \ No newline at end of file diff --git a/modules/local/porechop.nf b/modules/local/porechop.nf new file mode 100644 index 00000000..2ef954fb --- /dev/null +++ b/modules/local/porechop.nf @@ -0,0 +1,34 @@ +// Import generic module functions +include { initOptions; saveFiles; getSoftwareName } from './functions' + +params.options = [:] +options = initOptions(params.options) + +process PORECHOP { + tag "$meta.id" + label 'process_medium' + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), publish_id:'') } + + conda (params.enable_conda ? "porechop=0.2.4" : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://depot.galaxyproject.org/singularity/porechop:0.2.4--py38hed8969a_1" + } else { + container "quay.io/biocontainers/porechop:0.2.4--py38hed8969a_1" + } + + input: + tuple val(meta), path(reads) + + output: + tuple val(meta), path('trimmed.fastq'), emit: reads + path "*.version.txt" , emit: version + + script: + def software = getSoftwareName(task.process) + """ + porechop $options.args -i "${reads}" -t "${task.cpus}" -o trimmed.fastq + porechop --version > "${software}.version.txt" + """ +} diff --git a/modules/local/skewer.nf b/modules/local/skewer.nf index 3aa0bc72..2634806b 100644 --- a/modules/local/skewer.nf +++ b/modules/local/skewer.nf @@ -11,7 +11,7 @@ process SKEWER { mode: params.publish_dir_mode, saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), publish_id:'') } - conda (params.enable_conda ? "skewer:0.2.2-3" : null) + conda (params.enable_conda ? "skewer=0.2.2-3" : null) if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { container "https://depot.galaxyproject.org/singularity/skewer:0.2.2--hc9558a2_3" } else { diff --git a/modules/nf-core/modules/nanoplot/functions.nf b/modules/nf-core/modules/nanoplot/functions.nf new file mode 100644 index 00000000..da9da093 --- /dev/null +++ b/modules/nf-core/modules/nanoplot/functions.nf @@ -0,0 +1,68 @@ +// +// Utility functions used in nf-core DSL2 module files +// + +// +// Extract name of software tool from process name using $task.process +// +def getSoftwareName(task_process) { + return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() +} + +// +// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules +// +def initOptions(Map args) { + def Map options = [:] + options.args = args.args ?: '' + options.args2 = args.args2 ?: '' + options.args3 = args.args3 ?: '' + options.publish_by_meta = args.publish_by_meta ?: [] + options.publish_dir = args.publish_dir ?: '' + options.publish_files = args.publish_files + options.suffix = args.suffix ?: '' + return options +} + +// +// Tidy up and join elements of a list to return a path string +// +def getPathFromList(path_list) { + def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries + paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes + return paths.join('/') +} + +// +// Function to save/publish module results +// +def saveFiles(Map args) { + if (!args.filename.endsWith('.version.txt')) { + def ioptions = initOptions(args.options) + def path_list = [ ioptions.publish_dir ?: args.publish_dir ] + if (ioptions.publish_by_meta) { + def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta + for (key in key_list) { + if (args.meta && key instanceof String) { + def path = key + if (args.meta.containsKey(key)) { + path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] + } + path = path instanceof String ? path : '' + path_list.add(path) + } + } + } + if (ioptions.publish_files instanceof Map) { + for (ext in ioptions.publish_files) { + if (args.filename.endsWith(ext.key)) { + def ext_list = path_list.collect() + ext_list.add(ext.value) + return "${getPathFromList(ext_list)}/$args.filename" + } + } + } else if (ioptions.publish_files == null) { + return "${getPathFromList(path_list)}/$args.filename" + } + } +} diff --git a/modules/nf-core/modules/nanoplot/main.nf b/modules/nf-core/modules/nanoplot/main.nf new file mode 100644 index 00000000..f5fffe13 --- /dev/null +++ b/modules/nf-core/modules/nanoplot/main.nf @@ -0,0 +1,42 @@ +// Import generic module functions +include { initOptions; saveFiles; getSoftwareName } from './functions' + +params.options = [:] +options = initOptions(params.options) + +process NANOPLOT { + tag "$meta.id" + label 'process_low' + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } + + conda (params.enable_conda ? 'bioconda::nanoplot=1.38.0' : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://depot.galaxyproject.org/singularity/nanoplot:1.38.0--pyhdfd78af_0" + } else { + container "quay.io/biocontainers/nanoplot:1.38.0--pyhdfd78af_0" + } + + input: + tuple val(meta), path(ontfile) + + output: + tuple val(meta), path("*.html"), emit: html + tuple val(meta), path("*.png") , emit: png + tuple val(meta), path("*.txt") , emit: txt + tuple val(meta), path("*.log") , emit: log + path "*.version.txt" , emit: version + + script: + def software = getSoftwareName(task.process) + def input_file = ("$ontfile".endsWith(".fastq.gz")) ? "--fastq ${ontfile}" : + ("$ontfile".endsWith(".txt")) ? "--summary ${ontfile}" : '' + """ + NanoPlot \\ + $options.args \\ + -t $task.cpus \\ + $input_file + echo \$(NanoPlot --version 2>&1) | sed 's/^.*NanoPlot //; s/ .*\$//' > ${software}.version.txt + """ +} diff --git a/modules/nf-core/modules/nanoplot/meta.yml b/modules/nf-core/modules/nanoplot/meta.yml new file mode 100644 index 00000000..f1d94312 --- /dev/null +++ b/modules/nf-core/modules/nanoplot/meta.yml @@ -0,0 +1,58 @@ +name: nanoplot +description: Run NanoPlot on nanopore-sequenced reads +keywords: + - quality control + - qc + - fastq + - sequencing summary + - nanopore +tools: + - nanoplot: + description: | + NanoPlot is a tool for ploting long-read sequencing data and + alignment. + homepage: http://nanoplot.bioinf.be + documentation: https://github.com/wdecoster/NanoPlot +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - fastq: + type: file + description: | + List of input basecalled-FastQ files. + - summary_txt: + type: file + description: | + List of sequencing_summary.txt files from running basecalling. +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - html: + type: file + description: NanoPlot report + pattern: "*{.html}" + - png: + type: file + description: Plots generated by NanoPlot + pattern: "*{.png}" + - txt: + type: file + description: Stats from NanoPlot + pattern: "*{.txt}" + - log: + type: file + description: log file of NanoPlot run + pattern: "*{.log}" + - version: + type: file + description: File containing software version + pattern: "*.{version.txt}" +authors: + - "@drpatelh" + - "@yuukiiwa" diff --git a/modules/nf-core/modules/pycoqc/functions.nf b/modules/nf-core/modules/pycoqc/functions.nf new file mode 100644 index 00000000..da9da093 --- /dev/null +++ b/modules/nf-core/modules/pycoqc/functions.nf @@ -0,0 +1,68 @@ +// +// Utility functions used in nf-core DSL2 module files +// + +// +// Extract name of software tool from process name using $task.process +// +def getSoftwareName(task_process) { + return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() +} + +// +// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules +// +def initOptions(Map args) { + def Map options = [:] + options.args = args.args ?: '' + options.args2 = args.args2 ?: '' + options.args3 = args.args3 ?: '' + options.publish_by_meta = args.publish_by_meta ?: [] + options.publish_dir = args.publish_dir ?: '' + options.publish_files = args.publish_files + options.suffix = args.suffix ?: '' + return options +} + +// +// Tidy up and join elements of a list to return a path string +// +def getPathFromList(path_list) { + def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries + paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes + return paths.join('/') +} + +// +// Function to save/publish module results +// +def saveFiles(Map args) { + if (!args.filename.endsWith('.version.txt')) { + def ioptions = initOptions(args.options) + def path_list = [ ioptions.publish_dir ?: args.publish_dir ] + if (ioptions.publish_by_meta) { + def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta + for (key in key_list) { + if (args.meta && key instanceof String) { + def path = key + if (args.meta.containsKey(key)) { + path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] + } + path = path instanceof String ? path : '' + path_list.add(path) + } + } + } + if (ioptions.publish_files instanceof Map) { + for (ext in ioptions.publish_files) { + if (args.filename.endsWith(ext.key)) { + def ext_list = path_list.collect() + ext_list.add(ext.value) + return "${getPathFromList(ext_list)}/$args.filename" + } + } + } else if (ioptions.publish_files == null) { + return "${getPathFromList(path_list)}/$args.filename" + } + } +} diff --git a/modules/nf-core/modules/pycoqc/main.nf b/modules/nf-core/modules/pycoqc/main.nf new file mode 100644 index 00000000..3f010247 --- /dev/null +++ b/modules/nf-core/modules/pycoqc/main.nf @@ -0,0 +1,40 @@ +// Import generic module functions +include { initOptions; saveFiles; getSoftwareName } from './functions' + +params.options = [:] +options = initOptions(params.options) + +process PYCOQC { + tag "$summary" + label 'process_medium' + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:[:], publish_by_meta:[]) } + + conda (params.enable_conda ? "bioconda::pycoqc=2.5.2" : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://depot.galaxyproject.org/singularity/pycoqc:2.5.2--py_0" + } else { + container "quay.io/biocontainers/pycoqc:2.5.2--py_0" + } + + input: + path summary + + output: + path "*.html" , emit: html + path "*.json" , emit: json + path "*.version.txt", emit: version + + script: + def software = getSoftwareName(task.process) + """ + pycoQC \\ + $options.args \\ + -f $summary \\ + -o pycoqc.html \\ + -j pycoqc.json + + echo \$(pycoQC --version 2>&1) | sed 's/^.*pycoQC v//; s/ .*\$//' > ${software}.version.txt + """ +} diff --git a/modules/nf-core/modules/pycoqc/meta.yml b/modules/nf-core/modules/pycoqc/meta.yml new file mode 100644 index 00000000..059b2f15 --- /dev/null +++ b/modules/nf-core/modules/pycoqc/meta.yml @@ -0,0 +1,48 @@ +name: pycoqc +description: write your description here +keywords: + - qc + - quality control + - sequencing + - nanopore +tools: + - pycoqc: + description: PycoQC computes metrics and generates interactive QC plots for Oxford Nanopore technologies sequencing data + homepage: https://github.com/tleonardi/pycoQC + documentation: https://tleonardi.github.io/pycoQC/ + tool_dev_url: https://github.com/tleonardi/pycoQC + doi: "10.21105/joss.01236" + licence: ['GNU General Public v3 (GPL v3)'] + +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - summary: + type: file + description: sequencing summary file + pattern: "*.{txt}" + +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - html: + type: file + description: Results in HTML format + - json: + type: file + description: Results in JSON format + pattern: "*.{json}" + - version: + type: file + description: File containing software version + pattern: "*.{version.txt}" + +authors: + - "@joseespinosa" + - "@drpatelh" diff --git a/modules/nf-core/modules/spades/functions.nf b/modules/nf-core/modules/spades/functions.nf new file mode 100644 index 00000000..da9da093 --- /dev/null +++ b/modules/nf-core/modules/spades/functions.nf @@ -0,0 +1,68 @@ +// +// Utility functions used in nf-core DSL2 module files +// + +// +// Extract name of software tool from process name using $task.process +// +def getSoftwareName(task_process) { + return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() +} + +// +// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules +// +def initOptions(Map args) { + def Map options = [:] + options.args = args.args ?: '' + options.args2 = args.args2 ?: '' + options.args3 = args.args3 ?: '' + options.publish_by_meta = args.publish_by_meta ?: [] + options.publish_dir = args.publish_dir ?: '' + options.publish_files = args.publish_files + options.suffix = args.suffix ?: '' + return options +} + +// +// Tidy up and join elements of a list to return a path string +// +def getPathFromList(path_list) { + def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries + paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes + return paths.join('/') +} + +// +// Function to save/publish module results +// +def saveFiles(Map args) { + if (!args.filename.endsWith('.version.txt')) { + def ioptions = initOptions(args.options) + def path_list = [ ioptions.publish_dir ?: args.publish_dir ] + if (ioptions.publish_by_meta) { + def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta + for (key in key_list) { + if (args.meta && key instanceof String) { + def path = key + if (args.meta.containsKey(key)) { + path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] + } + path = path instanceof String ? path : '' + path_list.add(path) + } + } + } + if (ioptions.publish_files instanceof Map) { + for (ext in ioptions.publish_files) { + if (args.filename.endsWith(ext.key)) { + def ext_list = path_list.collect() + ext_list.add(ext.value) + return "${getPathFromList(ext_list)}/$args.filename" + } + } + } else if (ioptions.publish_files == null) { + return "${getPathFromList(path_list)}/$args.filename" + } + } +} diff --git a/modules/nf-core/modules/spades/main.nf b/modules/nf-core/modules/spades/main.nf new file mode 100644 index 00000000..58641617 --- /dev/null +++ b/modules/nf-core/modules/spades/main.nf @@ -0,0 +1,67 @@ +// Import generic module functions +include { initOptions; saveFiles; getSoftwareName } from './functions' + +params.options = [:] +options = initOptions(params.options) + +process SPADES { + //tag "$meta.id" + label 'process_high' + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } + + conda (params.enable_conda ? 'bioconda::spades=3.15.3' : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://depot.galaxyproject.org/singularity/spades:3.15.3--h95f258a_0" + } else { + container "quay.io/biocontainers/spades:3.15.3--h95f258a_0" + } + + input: + tuple val(meta), path(reads) + path hmm + + output: + tuple val(meta), path('*.scaffolds.fa') , optional:true, emit: scaffolds + tuple val(meta), path('*.contigs.fa') , optional:true, emit: contigs + tuple val(meta), path('*.transcripts.fa') , optional:true, emit: transcripts + tuple val(meta), path('*.gene_clusters.fa'), optional:true, emit: gene_clusters + tuple val(meta), path('*.assembly.gfa') , optional:true, emit: gfa + tuple val(meta), path('*.log') , emit: log + path '*.version.txt' , emit: version + + script: + def software = getSoftwareName(task.process) + def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" + def input_reads = meta.single_end ? "-s $reads" : "-1 ${reads[0]} -2 ${reads[1]}" + def custom_hmms = params.spades_hmm ? "--custom-hmms $hmm" : "" + """ + spades.py \\ + $options.args \\ + --threads $task.cpus \\ + $custom_hmms \\ + $input_reads \\ + -o ./ + mv spades.log ${prefix}.spades.log + + if [ -f scaffolds.fasta ]; then + mv scaffolds.fasta ${prefix}.scaffolds.fa + fi + if [ -f contigs.fasta ]; then + mv contigs.fasta ${prefix}.contigs.fa + fi + if [ -f transcripts.fasta ]; then + mv transcripts.fasta ${prefix}.transcripts.fa + fi + if [ -f assembly_graph_with_scaffolds.gfa ]; then + mv assembly_graph_with_scaffolds.gfa ${prefix}.assembly.gfa + fi + + if [ -f gene_clusters.fasta ]; then + mv gene_clusters.fasta ${prefix}.gene_clusters.fa + fi + + echo \$(spades.py --version 2>&1) | sed 's/^.*SPAdes genome assembler v//; s/ .*\$//' > ${software}.version.txt + """ +} diff --git a/modules/nf-core/modules/spades/meta.yml b/modules/nf-core/modules/spades/meta.yml new file mode 100644 index 00000000..5a05e5f3 --- /dev/null +++ b/modules/nf-core/modules/spades/meta.yml @@ -0,0 +1,69 @@ +name: spades +description: Assembles a small genome (bacterial, fungal, viral) +keywords: + - genome + - assembly + - genome assembler + - small genome + - de novo assembler +tools: + - spades: + description: SPAdes (St. Petersburg genome assembler) is intended for both standard isolates and single-cell MDA bacteria assemblies. + homepage: http://cab.spbu.ru/files/release3.15.0/manual.html + documentation: http://cab.spbu.ru/files/release3.15.0/manual.html + tool_dev_url: https://github.com/ablab/spades + doi: 10.1089/cmb.2012.0021 + licence: ['GPL v2'] +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - reads: + type: file + description: | + List of input FastQ files of size 1 and 2 for single-end and paired-end data, + respectively. + - hmm: + type: file + description: + File or directory with amino acid HMMs for Spades HMM-guided mode. +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - scaffolds: + type: file + description: | + Fasta file containing scaffolds + - contigs: + type: file + description: | + Fasta file containing contigs + - transcripts: + type: file + description: | + Fasta file containing transcripts + - gene_clusters: + type: file + description: | + Fasta file containing gene_clusters + - gfa: + type: file + description: | + gfa file containing assembly + - log: + type: file + description: | + Spades log file + - version: + type: file + description: File containing software version + pattern: "*.{version.txt}" + +authors: + - "@JoseEspinosa" + - "@drpatelh" diff --git a/modules/nf-core/modules/unicycler/functions.nf b/modules/nf-core/modules/unicycler/functions.nf new file mode 100644 index 00000000..da9da093 --- /dev/null +++ b/modules/nf-core/modules/unicycler/functions.nf @@ -0,0 +1,68 @@ +// +// Utility functions used in nf-core DSL2 module files +// + +// +// Extract name of software tool from process name using $task.process +// +def getSoftwareName(task_process) { + return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() +} + +// +// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules +// +def initOptions(Map args) { + def Map options = [:] + options.args = args.args ?: '' + options.args2 = args.args2 ?: '' + options.args3 = args.args3 ?: '' + options.publish_by_meta = args.publish_by_meta ?: [] + options.publish_dir = args.publish_dir ?: '' + options.publish_files = args.publish_files + options.suffix = args.suffix ?: '' + return options +} + +// +// Tidy up and join elements of a list to return a path string +// +def getPathFromList(path_list) { + def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries + paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes + return paths.join('/') +} + +// +// Function to save/publish module results +// +def saveFiles(Map args) { + if (!args.filename.endsWith('.version.txt')) { + def ioptions = initOptions(args.options) + def path_list = [ ioptions.publish_dir ?: args.publish_dir ] + if (ioptions.publish_by_meta) { + def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta + for (key in key_list) { + if (args.meta && key instanceof String) { + def path = key + if (args.meta.containsKey(key)) { + path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] + } + path = path instanceof String ? path : '' + path_list.add(path) + } + } + } + if (ioptions.publish_files instanceof Map) { + for (ext in ioptions.publish_files) { + if (args.filename.endsWith(ext.key)) { + def ext_list = path_list.collect() + ext_list.add(ext.value) + return "${getPathFromList(ext_list)}/$args.filename" + } + } + } else if (ioptions.publish_files == null) { + return "${getPathFromList(path_list)}/$args.filename" + } + } +} diff --git a/modules/nf-core/modules/unicycler/main.nf b/modules/nf-core/modules/unicycler/main.nf new file mode 100644 index 00000000..320c0f29 --- /dev/null +++ b/modules/nf-core/modules/unicycler/main.nf @@ -0,0 +1,47 @@ +// Import generic module functions +include { initOptions; saveFiles; getSoftwareName } from './functions' + +params.options = [:] +options = initOptions(params.options) + +process UNICYCLER { + tag "$meta.id" + label 'process_high' + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } + + conda (params.enable_conda ? 'bioconda::unicycler=0.4.8' : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://depot.galaxyproject.org/singularity/unicycler:0.4.8--py38h8162308_3" + } else { + container "quay.io/biocontainers/unicycler:0.4.8--py38h8162308_3" + } + + input: + tuple val(meta), path(reads) + + output: + tuple val(meta), path('*.scaffolds.fa'), emit: scaffolds + tuple val(meta), path('*.assembly.gfa'), emit: gfa + tuple val(meta), path('*.log') , emit: log + path '*.version.txt' , emit: version + + script: + def software = getSoftwareName(task.process) + def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" + def input_reads = meta.single_end ? "-s $reads" : "-1 ${reads[0]} -2 ${reads[1]}" + """ + unicycler \\ + --threads $task.cpus \\ + $options.args \\ + $input_reads \\ + --out ./ + + mv assembly.fasta ${prefix}.scaffolds.fa + mv assembly.gfa ${prefix}.assembly.gfa + mv unicycler.log ${prefix}.unicycler.log + + echo \$(unicycler --version 2>&1) | sed 's/^.*Unicycler v//; s/ .*\$//' > ${software}.version.txt + """ +} diff --git a/modules/nf-core/modules/unicycler/meta.yml b/modules/nf-core/modules/unicycler/meta.yml new file mode 100644 index 00000000..286b7f67 --- /dev/null +++ b/modules/nf-core/modules/unicycler/meta.yml @@ -0,0 +1,55 @@ +name: unicycler +description: Assembles bacterial genomes +keywords: + - genome + - assembly + - genome assembler + - small genome +tools: + - unicycler: + description: Hybrid assembly pipeline for bacterial genomes + homepage: https://github.com/rrwick/Unicycler + documentation: https://github.com/rrwick/Unicycler + tool_dev_url: https://github.com/rrwick/Unicycler + doi: 10.1371/journal.pcbi.1005595 + licence: ['GPL v3'] +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - reads: + type: file + description: | + List of input FastQ files of size 1 and 2 for single-end and paired-end data, + respectively. +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - version: + type: file + description: File containing software version + pattern: "*.{version.txt}" + - scaffolds: + type: file + description: Fasta file containing scaffolds + pattern: "*.{scaffolds.fa}" + - gfa: + type: file + description: gfa file containing assembly + pattern: "*.{assembly.gfa}" + - log: + type: file + description: unicycler log file + pattern: "*.{log}" + - version: + type: file + description: File containing software version + pattern: "*.{version.txt}" +authors: + - "@JoseEspinosa" + - "@drpatelh" diff --git a/subworkflows/local/input_check.nf b/subworkflows/local/input_check.nf index 16f36459..37c23265 100644 --- a/subworkflows/local/input_check.nf +++ b/subworkflows/local/input_check.nf @@ -22,14 +22,19 @@ workflow INPUT_CHECK { .filter{ meta, reads -> reads != 'NA' } // TODO: filter when PE and 'NA' .set { shortreads } reads - .map { meta, reads, long_fastq, fast5 -> [ meta, long_fastq, fast5 ] } - .filter{ meta, long_fastq, fast5 -> long_fastq != 'NA' && fast5 != 'NA' } + .map { meta, reads, long_fastq, fast5 -> [ meta, long_fastq ] } + .filter{ meta, long_fastq -> long_fastq != 'NA' } .set { longreads } + reads + .map { meta, reads, long_fastq, fast5 -> [ meta, fast5 ] } + .filter{ meta, fast5 -> fast5 != 'NA' } + .set { fast5 } emit: reads // channel: [ val(meta), [ reads ], long_fastq, fast5 ] shortreads // channel: [ val(meta), [ reads ] ] - longreads // channel: [ val(meta), long_fastq, fast5 ] + longreads // channel: [ val(meta), long_fastq ] + fast5 // channel: [ val(meta), fast5 ] } // Function to get list of [ meta, [ fastq_1, fastq_2 ], long_fastq, fast5 ] diff --git a/workflows/bacass.nf b/workflows/bacass.nf index aab8ccfc..866e9d56 100644 --- a/workflows/bacass.nf +++ b/workflows/bacass.nf @@ -39,7 +39,8 @@ def modules = params.modules.clone() // MODULE: Local to the pipeline // include { GET_SOFTWARE_VERSIONS } from '../modules/local/get_software_versions' addParams( options: [publish_files : ['tsv':'']] ) -include { SKEWER } from '../modules/local/skewer' addParams( options: modules['skewer'] ) +include { SKEWER } from '../modules/local/skewer' addParams( options: modules['skewer'] ) +include { PORECHOP } from '../modules/local/porechop' addParams( options: modules['porechop'] ) // // SUBWORKFLOW: Consisting of a mix of local and nf-core/modules @@ -58,8 +59,12 @@ multiqc_options.args += params.multiqc_title ? Utils.joinModuleArgs(["--title \" // // MODULE: Installed directly from nf-core/modules // -include { FASTQC } from '../modules/nf-core/modules/fastqc/main' addParams( options: modules['fastqc'] ) -include { MULTIQC } from '../modules/nf-core/modules/multiqc/main' addParams( options: multiqc_options ) +include { FASTQC } from '../modules/nf-core/modules/fastqc/main' addParams( options: modules['fastqc'] ) +include { NANOPLOT } from '../modules/nf-core/modules/nanoplot/main' addParams( options: modules['nanoplot'] ) +include { PYCOQC } from '../modules/nf-core/modules/pycoqc/main' addParams( options: modules['pycoqc'] ) +include { UNICYCLER } from '../modules/nf-core/modules/unicycler/main' addParams( options: modules['unicycler'] ) +include { SPADES } from '../modules/nf-core/modules/spades/main' addParams( options: modules['spades'] ) +include { MULTIQC } from '../modules/nf-core/modules/multiqc/main' addParams( options: multiqc_options ) /* ======================================================================================== @@ -90,13 +95,84 @@ workflow BACASS { ch_software_versions = ch_software_versions.mix(FASTQC.out.version.first().ifEmpty(null)) // - // MODULE: Run Skewer, trim and combine short read read-pairs per sample. Similar to nf-core vipr + // MODULE: Skewer, trim and combine short read read-pairs per sample. Similar to nf-core vipr // SKEWER ( INPUT_CHECK.out.shortreads ) //ch_software_versions = ch_software_versions.mix(SKEWER.out.version.first().ifEmpty(null)) //TODO + // + // MODULE: Nanoplot, quality check for nanopore reads and Quality/Length Plots + // + NANOPLOT ( + INPUT_CHECK.out.longreads + ) + ch_software_versions = ch_software_versions.mix(NANOPLOT.out.version.first().ifEmpty(null)) + + // + // MODULE: PYCOQC, quality check for nanopore reads and Quality/Length Plots + // + PYCOQC ( + INPUT_CHECK.out.fast5 + ) + ch_software_versions = ch_software_versions.mix(PYCOQC.out.version.first().ifEmpty(null)) + + // + // MODULE: PYCOQC, quality check for nanopore reads and Quality/Length Plots + // + + // TODO: if ( params.assembly_type == 'hybrid' || params.assembly_type == 'long' && !('short' in params.assembly_type) ) + PORECHOP ( + INPUT_CHECK.out.longreads + ) + ch_software_versions = ch_software_versions.mix(PORECHOP.out.version.first().ifEmpty(null)) + + // + // MODULE: Unicycler, nf-core module allows only short assembly assembly + // + // TODO: if ( params.assembler == 'unicycler' ) { + + UNICYCLER ( + SKEWER.out.reads + ) + ch_software_versions = ch_software_versions.mix(UNICYCLER.out.version.first().ifEmpty(null)) +/* + // + // MODULE: SPAdes, nf-core module allows only short read assembly + // + // TODO: if ( params.assembler == 'spades' ) { + // TODO: error: java.lang.NullPointerException: Cannot get property 'id' on null object + // TODO: probabaly: params.spades_hmm = false? + params.spades_hmm = false + SPADES ( + SKEWER.out.reads, params.spades_hmm + ) + ch_software_versions = ch_software_versions.mix(SPADES.out.version.first().ifEmpty(null)) +*/ + + // + // Join channels for unicycler, as trimming the files happens in two separate processes for paralellization of individual steps. As samples have the same sampleID, we can simply use join() to merge the channels based on this. If we only have one of the channels we insert 'NAs' which are not used in the unicycler process then subsequently, in case of short or long read only assembly. + // TODO: +/* + if(params.assembly_type == 'hybrid'){ + SKEWER.out.reads + .join(PORECHOP.out.reads) + .dump(tag: 'unicycler') + .set {ch_unicycler} + } else if(params.assembly_type == 'short'){ + SKEWER.out.reads + .map{ id,R1,R2 -> tuple(id,R1,R2,'NA') } + .dump(tag: 'unicycler') + .set {ch_unicycler} + } else if(params.assembly_type == 'long'){ + ch_long_trimmed_unicycler + .map{ id,lr -> tuple(id,'NA','NA',lr) } + .dump(tag: 'unicycler') + .set {ch_unicycler} //old channel name: ch_short_long_joint_unicycler + } +*/ + // // MODULE: Pipeline reporting // From 0941b473055f15364a818eeb2bd51ee8926d01f3 Mon Sep 17 00:00:00 2001 From: d4straub Date: Thu, 5 Aug 2021 17:25:57 +0200 Subject: [PATCH 22/81] from samplesheet csv to unicycler channels all fine --- bin/check_samplesheet.py | 17 ++-- conf/modules.config | 4 - modules.json | 9 -- .../nanoplot/main.nf => local/nanoplot.nf} | 3 +- .../unicycler/main.nf => local/unicycler.nf} | 10 ++- modules/nf-core/modules/nanoplot/functions.nf | 68 --------------- modules/nf-core/modules/nanoplot/meta.yml | 58 ------------- modules/nf-core/modules/spades/functions.nf | 68 --------------- modules/nf-core/modules/spades/main.nf | 67 -------------- modules/nf-core/modules/spades/meta.yml | 69 --------------- .../nf-core/modules/unicycler/functions.nf | 68 --------------- modules/nf-core/modules/unicycler/meta.yml | 55 ------------ subworkflows/local/input_check.nf | 44 ++++++---- workflows/bacass.nf | 87 +++++++++---------- 14 files changed, 85 insertions(+), 542 deletions(-) rename modules/{nf-core/modules/nanoplot/main.nf => local/nanoplot.nf} (90%) rename modules/{nf-core/modules/unicycler/main.nf => local/unicycler.nf} (81%) delete mode 100644 modules/nf-core/modules/nanoplot/functions.nf delete mode 100644 modules/nf-core/modules/nanoplot/meta.yml delete mode 100644 modules/nf-core/modules/spades/functions.nf delete mode 100644 modules/nf-core/modules/spades/main.nf delete mode 100644 modules/nf-core/modules/spades/meta.yml delete mode 100644 modules/nf-core/modules/unicycler/functions.nf delete mode 100644 modules/nf-core/modules/unicycler/meta.yml diff --git a/bin/check_samplesheet.py b/bin/check_samplesheet.py index 310bdf0e..cef3e4fe 100755 --- a/bin/check_samplesheet.py +++ b/bin/check_samplesheet.py @@ -43,12 +43,12 @@ def check_samplesheet(file_in, file_out): """ This function checks that the samplesheet follows the following structure: - sample,fastq_1,fastq_2,long_fastq,fast5,genome_size - SAMPLE_PE,SAMPLE_PE_1.fastq.gz,SAMPLE_PE_2.fastq.gz,SAMPLE_LONG.fastq.gz,SAMPLE_LONG.fast5,2.8m - SAMPLE_PE,SAMPLE_PE_1.fastq.gz,SAMPLE_PE_2.fastq.gz,NA,NA,2.8m + sample,fastq_1,fastq_2 + SAMPLE_PE,SAMPLE_PE_RUN1_1.fastq.gz,SAMPLE_PE_RUN1_2.fastq.gz + SAMPLE_PE,SAMPLE_PE_RUN2_1.fastq.gz,SAMPLE_PE_RUN2_2.fastq.gz + SAMPLE_SE,SAMPLE_SE_RUN1_1.fastq.gz, For an example see: - TODO: link actual example https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv """ @@ -56,8 +56,9 @@ def check_samplesheet(file_in, file_out): with open(file_in, "r") as fin: ## Check header - MIN_COLS = 6 - HEADER = ["sample", "fastq_1", "fastq_2", "long_fastq", "fast5", "genome_size"] + MIN_COLS = 2 + # TODO nf-core: Update the column names for the input samplesheet + HEADER = ["sample", "fastq_1", "fastq_2"] header = [x.strip('"') for x in fin.readline().strip().split(",")] if header[: len(HEADER)] != HEADER: print("ERROR: Please check samplesheet header -> {} != {}".format(",".join(header), ",".join(HEADER))) @@ -83,7 +84,7 @@ def check_samplesheet(file_in, file_out): ) ## Check sample name entries - sample, fastq_1, fastq_2, long_fastq, fast5, genome_size = lspl[: len(HEADER)] + sample, fastq_1, fastq_2 = lspl[: len(HEADER)] sample = sample.replace(" ", "_") if not sample: print_error("Sample entry has not been specified!", "Line", line) @@ -123,7 +124,7 @@ def check_samplesheet(file_in, file_out): out_dir = os.path.dirname(file_out) make_dir(out_dir) with open(file_out, "w") as fout: - fout.write(",".join(["sample", "single_end", "fastq_1", "fastq_2", "long_fastq", "fast5", "genome_size"]) + "\n") + fout.write(",".join(["sample", "single_end", "fastq_1", "fastq_2"]) + "\n") for sample in sorted(sample_mapping_dict.keys()): ## Check that multiple runs of the same sample are of the same datatype diff --git a/conf/modules.config b/conf/modules.config index 14bb820f..e133b9b8 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -46,10 +46,6 @@ params { args = "" publish_dir = "./Unicycler" } - 'spades' { - args = "" - publish_dir = "./SPAdes" - } 'multiqc' { args = "" } diff --git a/modules.json b/modules.json index 051ac69c..ae418c41 100644 --- a/modules.json +++ b/modules.json @@ -9,17 +9,8 @@ "multiqc": { "git_sha": "e937c7950af70930d1f34bb961403d9d2aa81c7d" }, - "nanoplot": { - "git_sha": "c5235a983d454787fa0c3247b02086969217163b" - }, "pycoqc": { "git_sha": "e937c7950af70930d1f34bb961403d9d2aa81c7d" - }, - "spades": { - "git_sha": "c5235a983d454787fa0c3247b02086969217163b" - }, - "unicycler": { - "git_sha": "e937c7950af70930d1f34bb961403d9d2aa81c7d" } } } diff --git a/modules/nf-core/modules/nanoplot/main.nf b/modules/local/nanoplot.nf similarity index 90% rename from modules/nf-core/modules/nanoplot/main.nf rename to modules/local/nanoplot.nf index f5fffe13..a0540aba 100644 --- a/modules/nf-core/modules/nanoplot/main.nf +++ b/modules/local/nanoplot.nf @@ -30,8 +30,7 @@ process NANOPLOT { script: def software = getSoftwareName(task.process) - def input_file = ("$ontfile".endsWith(".fastq.gz")) ? "--fastq ${ontfile}" : - ("$ontfile".endsWith(".txt")) ? "--summary ${ontfile}" : '' + def input_file = "--fastq ${ontfile}" """ NanoPlot \\ $options.args \\ diff --git a/modules/nf-core/modules/unicycler/main.nf b/modules/local/unicycler.nf similarity index 81% rename from modules/nf-core/modules/unicycler/main.nf rename to modules/local/unicycler.nf index 320c0f29..e2cf4cec 100644 --- a/modules/nf-core/modules/unicycler/main.nf +++ b/modules/local/unicycler.nf @@ -19,7 +19,7 @@ process UNICYCLER { } input: - tuple val(meta), path(reads) + tuple val(meta), file(reads), file(longreads) output: tuple val(meta), path('*.scaffolds.fa'), emit: scaffolds @@ -30,7 +30,13 @@ process UNICYCLER { script: def software = getSoftwareName(task.process) def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" - def input_reads = meta.single_end ? "-s $reads" : "-1 ${reads[0]} -2 ${reads[1]}" + if(params.assembly_type == 'long'){ + input_reads = "-l $longreads" + } else if (params.assembly_type == 'short'){ + input_reads = "-1 ${reads[0]} -2 ${reads[1]}" + } else if (params.assembly_type == 'hybrid'){ + input_reads = "-1 ${reads[0]} -2 ${reads[1]} -l $longreads" + } """ unicycler \\ --threads $task.cpus \\ diff --git a/modules/nf-core/modules/nanoplot/functions.nf b/modules/nf-core/modules/nanoplot/functions.nf deleted file mode 100644 index da9da093..00000000 --- a/modules/nf-core/modules/nanoplot/functions.nf +++ /dev/null @@ -1,68 +0,0 @@ -// -// Utility functions used in nf-core DSL2 module files -// - -// -// Extract name of software tool from process name using $task.process -// -def getSoftwareName(task_process) { - return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() -} - -// -// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules -// -def initOptions(Map args) { - def Map options = [:] - options.args = args.args ?: '' - options.args2 = args.args2 ?: '' - options.args3 = args.args3 ?: '' - options.publish_by_meta = args.publish_by_meta ?: [] - options.publish_dir = args.publish_dir ?: '' - options.publish_files = args.publish_files - options.suffix = args.suffix ?: '' - return options -} - -// -// Tidy up and join elements of a list to return a path string -// -def getPathFromList(path_list) { - def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries - paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes - return paths.join('/') -} - -// -// Function to save/publish module results -// -def saveFiles(Map args) { - if (!args.filename.endsWith('.version.txt')) { - def ioptions = initOptions(args.options) - def path_list = [ ioptions.publish_dir ?: args.publish_dir ] - if (ioptions.publish_by_meta) { - def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta - for (key in key_list) { - if (args.meta && key instanceof String) { - def path = key - if (args.meta.containsKey(key)) { - path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] - } - path = path instanceof String ? path : '' - path_list.add(path) - } - } - } - if (ioptions.publish_files instanceof Map) { - for (ext in ioptions.publish_files) { - if (args.filename.endsWith(ext.key)) { - def ext_list = path_list.collect() - ext_list.add(ext.value) - return "${getPathFromList(ext_list)}/$args.filename" - } - } - } else if (ioptions.publish_files == null) { - return "${getPathFromList(path_list)}/$args.filename" - } - } -} diff --git a/modules/nf-core/modules/nanoplot/meta.yml b/modules/nf-core/modules/nanoplot/meta.yml deleted file mode 100644 index f1d94312..00000000 --- a/modules/nf-core/modules/nanoplot/meta.yml +++ /dev/null @@ -1,58 +0,0 @@ -name: nanoplot -description: Run NanoPlot on nanopore-sequenced reads -keywords: - - quality control - - qc - - fastq - - sequencing summary - - nanopore -tools: - - nanoplot: - description: | - NanoPlot is a tool for ploting long-read sequencing data and - alignment. - homepage: http://nanoplot.bioinf.be - documentation: https://github.com/wdecoster/NanoPlot -input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - fastq: - type: file - description: | - List of input basecalled-FastQ files. - - summary_txt: - type: file - description: | - List of sequencing_summary.txt files from running basecalling. -output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - html: - type: file - description: NanoPlot report - pattern: "*{.html}" - - png: - type: file - description: Plots generated by NanoPlot - pattern: "*{.png}" - - txt: - type: file - description: Stats from NanoPlot - pattern: "*{.txt}" - - log: - type: file - description: log file of NanoPlot run - pattern: "*{.log}" - - version: - type: file - description: File containing software version - pattern: "*.{version.txt}" -authors: - - "@drpatelh" - - "@yuukiiwa" diff --git a/modules/nf-core/modules/spades/functions.nf b/modules/nf-core/modules/spades/functions.nf deleted file mode 100644 index da9da093..00000000 --- a/modules/nf-core/modules/spades/functions.nf +++ /dev/null @@ -1,68 +0,0 @@ -// -// Utility functions used in nf-core DSL2 module files -// - -// -// Extract name of software tool from process name using $task.process -// -def getSoftwareName(task_process) { - return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() -} - -// -// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules -// -def initOptions(Map args) { - def Map options = [:] - options.args = args.args ?: '' - options.args2 = args.args2 ?: '' - options.args3 = args.args3 ?: '' - options.publish_by_meta = args.publish_by_meta ?: [] - options.publish_dir = args.publish_dir ?: '' - options.publish_files = args.publish_files - options.suffix = args.suffix ?: '' - return options -} - -// -// Tidy up and join elements of a list to return a path string -// -def getPathFromList(path_list) { - def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries - paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes - return paths.join('/') -} - -// -// Function to save/publish module results -// -def saveFiles(Map args) { - if (!args.filename.endsWith('.version.txt')) { - def ioptions = initOptions(args.options) - def path_list = [ ioptions.publish_dir ?: args.publish_dir ] - if (ioptions.publish_by_meta) { - def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta - for (key in key_list) { - if (args.meta && key instanceof String) { - def path = key - if (args.meta.containsKey(key)) { - path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] - } - path = path instanceof String ? path : '' - path_list.add(path) - } - } - } - if (ioptions.publish_files instanceof Map) { - for (ext in ioptions.publish_files) { - if (args.filename.endsWith(ext.key)) { - def ext_list = path_list.collect() - ext_list.add(ext.value) - return "${getPathFromList(ext_list)}/$args.filename" - } - } - } else if (ioptions.publish_files == null) { - return "${getPathFromList(path_list)}/$args.filename" - } - } -} diff --git a/modules/nf-core/modules/spades/main.nf b/modules/nf-core/modules/spades/main.nf deleted file mode 100644 index 58641617..00000000 --- a/modules/nf-core/modules/spades/main.nf +++ /dev/null @@ -1,67 +0,0 @@ -// Import generic module functions -include { initOptions; saveFiles; getSoftwareName } from './functions' - -params.options = [:] -options = initOptions(params.options) - -process SPADES { - //tag "$meta.id" - label 'process_high' - publishDir "${params.outdir}", - mode: params.publish_dir_mode, - saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } - - conda (params.enable_conda ? 'bioconda::spades=3.15.3' : null) - if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://depot.galaxyproject.org/singularity/spades:3.15.3--h95f258a_0" - } else { - container "quay.io/biocontainers/spades:3.15.3--h95f258a_0" - } - - input: - tuple val(meta), path(reads) - path hmm - - output: - tuple val(meta), path('*.scaffolds.fa') , optional:true, emit: scaffolds - tuple val(meta), path('*.contigs.fa') , optional:true, emit: contigs - tuple val(meta), path('*.transcripts.fa') , optional:true, emit: transcripts - tuple val(meta), path('*.gene_clusters.fa'), optional:true, emit: gene_clusters - tuple val(meta), path('*.assembly.gfa') , optional:true, emit: gfa - tuple val(meta), path('*.log') , emit: log - path '*.version.txt' , emit: version - - script: - def software = getSoftwareName(task.process) - def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" - def input_reads = meta.single_end ? "-s $reads" : "-1 ${reads[0]} -2 ${reads[1]}" - def custom_hmms = params.spades_hmm ? "--custom-hmms $hmm" : "" - """ - spades.py \\ - $options.args \\ - --threads $task.cpus \\ - $custom_hmms \\ - $input_reads \\ - -o ./ - mv spades.log ${prefix}.spades.log - - if [ -f scaffolds.fasta ]; then - mv scaffolds.fasta ${prefix}.scaffolds.fa - fi - if [ -f contigs.fasta ]; then - mv contigs.fasta ${prefix}.contigs.fa - fi - if [ -f transcripts.fasta ]; then - mv transcripts.fasta ${prefix}.transcripts.fa - fi - if [ -f assembly_graph_with_scaffolds.gfa ]; then - mv assembly_graph_with_scaffolds.gfa ${prefix}.assembly.gfa - fi - - if [ -f gene_clusters.fasta ]; then - mv gene_clusters.fasta ${prefix}.gene_clusters.fa - fi - - echo \$(spades.py --version 2>&1) | sed 's/^.*SPAdes genome assembler v//; s/ .*\$//' > ${software}.version.txt - """ -} diff --git a/modules/nf-core/modules/spades/meta.yml b/modules/nf-core/modules/spades/meta.yml deleted file mode 100644 index 5a05e5f3..00000000 --- a/modules/nf-core/modules/spades/meta.yml +++ /dev/null @@ -1,69 +0,0 @@ -name: spades -description: Assembles a small genome (bacterial, fungal, viral) -keywords: - - genome - - assembly - - genome assembler - - small genome - - de novo assembler -tools: - - spades: - description: SPAdes (St. Petersburg genome assembler) is intended for both standard isolates and single-cell MDA bacteria assemblies. - homepage: http://cab.spbu.ru/files/release3.15.0/manual.html - documentation: http://cab.spbu.ru/files/release3.15.0/manual.html - tool_dev_url: https://github.com/ablab/spades - doi: 10.1089/cmb.2012.0021 - licence: ['GPL v2'] -input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - reads: - type: file - description: | - List of input FastQ files of size 1 and 2 for single-end and paired-end data, - respectively. - - hmm: - type: file - description: - File or directory with amino acid HMMs for Spades HMM-guided mode. -output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - scaffolds: - type: file - description: | - Fasta file containing scaffolds - - contigs: - type: file - description: | - Fasta file containing contigs - - transcripts: - type: file - description: | - Fasta file containing transcripts - - gene_clusters: - type: file - description: | - Fasta file containing gene_clusters - - gfa: - type: file - description: | - gfa file containing assembly - - log: - type: file - description: | - Spades log file - - version: - type: file - description: File containing software version - pattern: "*.{version.txt}" - -authors: - - "@JoseEspinosa" - - "@drpatelh" diff --git a/modules/nf-core/modules/unicycler/functions.nf b/modules/nf-core/modules/unicycler/functions.nf deleted file mode 100644 index da9da093..00000000 --- a/modules/nf-core/modules/unicycler/functions.nf +++ /dev/null @@ -1,68 +0,0 @@ -// -// Utility functions used in nf-core DSL2 module files -// - -// -// Extract name of software tool from process name using $task.process -// -def getSoftwareName(task_process) { - return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() -} - -// -// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules -// -def initOptions(Map args) { - def Map options = [:] - options.args = args.args ?: '' - options.args2 = args.args2 ?: '' - options.args3 = args.args3 ?: '' - options.publish_by_meta = args.publish_by_meta ?: [] - options.publish_dir = args.publish_dir ?: '' - options.publish_files = args.publish_files - options.suffix = args.suffix ?: '' - return options -} - -// -// Tidy up and join elements of a list to return a path string -// -def getPathFromList(path_list) { - def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries - paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes - return paths.join('/') -} - -// -// Function to save/publish module results -// -def saveFiles(Map args) { - if (!args.filename.endsWith('.version.txt')) { - def ioptions = initOptions(args.options) - def path_list = [ ioptions.publish_dir ?: args.publish_dir ] - if (ioptions.publish_by_meta) { - def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta - for (key in key_list) { - if (args.meta && key instanceof String) { - def path = key - if (args.meta.containsKey(key)) { - path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] - } - path = path instanceof String ? path : '' - path_list.add(path) - } - } - } - if (ioptions.publish_files instanceof Map) { - for (ext in ioptions.publish_files) { - if (args.filename.endsWith(ext.key)) { - def ext_list = path_list.collect() - ext_list.add(ext.value) - return "${getPathFromList(ext_list)}/$args.filename" - } - } - } else if (ioptions.publish_files == null) { - return "${getPathFromList(path_list)}/$args.filename" - } - } -} diff --git a/modules/nf-core/modules/unicycler/meta.yml b/modules/nf-core/modules/unicycler/meta.yml deleted file mode 100644 index 286b7f67..00000000 --- a/modules/nf-core/modules/unicycler/meta.yml +++ /dev/null @@ -1,55 +0,0 @@ -name: unicycler -description: Assembles bacterial genomes -keywords: - - genome - - assembly - - genome assembler - - small genome -tools: - - unicycler: - description: Hybrid assembly pipeline for bacterial genomes - homepage: https://github.com/rrwick/Unicycler - documentation: https://github.com/rrwick/Unicycler - tool_dev_url: https://github.com/rrwick/Unicycler - doi: 10.1371/journal.pcbi.1005595 - licence: ['GPL v3'] -input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - reads: - type: file - description: | - List of input FastQ files of size 1 and 2 for single-end and paired-end data, - respectively. -output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - version: - type: file - description: File containing software version - pattern: "*.{version.txt}" - - scaffolds: - type: file - description: Fasta file containing scaffolds - pattern: "*.{scaffolds.fa}" - - gfa: - type: file - description: gfa file containing assembly - pattern: "*.{assembly.gfa}" - - log: - type: file - description: unicycler log file - pattern: "*.{log}" - - version: - type: file - description: File containing software version - pattern: "*.{version.txt}" -authors: - - "@JoseEspinosa" - - "@drpatelh" diff --git a/subworkflows/local/input_check.nf b/subworkflows/local/input_check.nf index 37c23265..043578bb 100644 --- a/subworkflows/local/input_check.nf +++ b/subworkflows/local/input_check.nf @@ -11,7 +11,9 @@ workflow INPUT_CHECK { samplesheet // file: /path/to/samplesheet.csv main: - SAMPLESHEET_CHECK ( samplesheet ) + Channel + .fromPath( samplesheet ) + .ifEmpty {exit 1, log.info "Cannot find path file ${tsvFile}"} .splitCsv ( header:true, sep:',' ) .map { create_fastq_channels(it) } .set { reads } @@ -19,7 +21,8 @@ workflow INPUT_CHECK { // reconfigure channels reads .map { meta, reads, long_fastq, fast5 -> [ meta, reads ] } - .filter{ meta, reads -> reads != 'NA' } // TODO: filter when PE and 'NA' + .filter{ meta, reads -> reads != 'NA' } + .filter{ meta, reads -> reads[0] != 'NA' && reads[1] != 'NA' } .set { shortreads } reads .map { meta, reads, long_fastq, fast5 -> [ meta, long_fastq ] } @@ -41,38 +44,45 @@ workflow INPUT_CHECK { def create_fastq_channels(LinkedHashMap row) { def meta = [:] meta.id = row.sample - meta.single_end = row.single_end.toBoolean() + meta.single_end = false meta.genome_size = row.genome_size == null ? 'NA' : row.genome_size def array = [] - if (!file(row.fastq_1).exists()) { - exit 1, "ERROR: Please check input samplesheet -> Read 1 FastQ file does not exist!\n${row.fastq_1}" - } + // check short reads + if ( !(row.fastq_1 == 'NA') ) { + if ( !file(row.fastq_1).exists() ) { + exit 1, "ERROR: Please check input samplesheet -> Read 1 FastQ file does not exist!\n${row.fastq_1}" + } + fastq_1 = file(row.fastq_1) + } else { fastq_1 = 'NA' } + if ( !(row.fastq_2 == 'NA') ) { + if ( !file(row.fastq_2).exists() ) { + exit 1, "ERROR: Please check input samplesheet -> Read 2 FastQ file does not exist!\n${row.fastq_2}" + } + fastq_2 = file(row.fastq_2) + } else { fastq_2 = 'NA' } // check long_fastq - if (!row.long_fastq == 'NA') { - if (!file(row.long_fastq).exists()) { + if ( !(row.long_fastq == 'NA') ) { + if ( !file(row.long_fastq).exists() ) { exit 1, "ERROR: Please check input samplesheet -> Long FastQ file does not exist!\n${row.fastq_1}" } long_fastq = file(row.long_fastq) } else { long_fastq = 'NA' } // check long_fastq - if (!row.fast5 == 'NA') { - if (!file(row.fast5).exists()) { + if ( !(row.fast5 == 'NA') ) { + if ( !file(row.fast5).exists() ) { exit 1, "ERROR: Please check input samplesheet -> Fast5 file does not exist!\n${row.fastq_1}" } fast5 = file(row.fast5) } else { fast5 = 'NA' } - // prepare output - if (meta.single_end) { - array = [ meta, [ file(row.fastq_1) ], long_fastq, fast5 ] + // prepare output // currently does not allow single end data! + if ( meta.single_end ) { + array = [ meta, fastq_1 , long_fastq, fast5 ] } else { - if (!file(row.fastq_2).exists()) { - exit 1, "ERROR: Please check input samplesheet -> Read 2 FastQ file does not exist!\n${row.fastq_2}" - } - array = [ meta, [ file(row.fastq_1), file(row.fastq_2) ], long_fastq, fast5 ] + array = [ meta, [ fastq_1, fastq_2 ], long_fastq, fast5 ] } return array } diff --git a/workflows/bacass.nf b/workflows/bacass.nf index 866e9d56..aa21118a 100644 --- a/workflows/bacass.nf +++ b/workflows/bacass.nf @@ -35,12 +35,17 @@ ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath(params.multi // Don't overwrite global params.modules, create a copy instead and use that within the main script. def modules = params.modules.clone() +def unicycler_options = modules['unicycler'] +unicycler_options.args += " $params.unicycler_args" + // // MODULE: Local to the pipeline // -include { GET_SOFTWARE_VERSIONS } from '../modules/local/get_software_versions' addParams( options: [publish_files : ['tsv':'']] ) -include { SKEWER } from '../modules/local/skewer' addParams( options: modules['skewer'] ) -include { PORECHOP } from '../modules/local/porechop' addParams( options: modules['porechop'] ) +include { GET_SOFTWARE_VERSIONS } from '../modules/local/get_software_versions' addParams( options: [publish_files : ['tsv':'']] ) +include { SKEWER } from '../modules/local/skewer' addParams( options: modules['skewer'] ) +include { NANOPLOT } from '../modules/local/nanoplot' addParams( options: modules['nanoplot'] ) +include { PORECHOP } from '../modules/local/porechop' addParams( options: modules['porechop'] ) +include { UNICYCLER } from '../modules/local/unicycler' addParams( options: unicycler_options ) // // SUBWORKFLOW: Consisting of a mix of local and nf-core/modules @@ -60,10 +65,7 @@ multiqc_options.args += params.multiqc_title ? Utils.joinModuleArgs(["--title \" // MODULE: Installed directly from nf-core/modules // include { FASTQC } from '../modules/nf-core/modules/fastqc/main' addParams( options: modules['fastqc'] ) -include { NANOPLOT } from '../modules/nf-core/modules/nanoplot/main' addParams( options: modules['nanoplot'] ) include { PYCOQC } from '../modules/nf-core/modules/pycoqc/main' addParams( options: modules['pycoqc'] ) -include { UNICYCLER } from '../modules/nf-core/modules/unicycler/main' addParams( options: modules['unicycler'] ) -include { SPADES } from '../modules/nf-core/modules/spades/main' addParams( options: modules['spades'] ) include { MULTIQC } from '../modules/nf-core/modules/multiqc/main' addParams( options: multiqc_options ) /* @@ -85,7 +87,7 @@ workflow BACASS { INPUT_CHECK ( ch_input ) - + // // MODULE: Run FastQC // @@ -98,7 +100,7 @@ workflow BACASS { // MODULE: Skewer, trim and combine short read read-pairs per sample. Similar to nf-core vipr // SKEWER ( - INPUT_CHECK.out.shortreads + INPUT_CHECK.out.shortreads.dump(tag: 'shortreads') ) //ch_software_versions = ch_software_versions.mix(SKEWER.out.version.first().ifEmpty(null)) //TODO @@ -113,10 +115,12 @@ workflow BACASS { // // MODULE: PYCOQC, quality check for nanopore reads and Quality/Length Plots // - PYCOQC ( - INPUT_CHECK.out.fast5 - ) - ch_software_versions = ch_software_versions.mix(PYCOQC.out.version.first().ifEmpty(null)) + if ( !params.skip_pycoqc ) { + PYCOQC ( + INPUT_CHECK.out.fast5.dump(tag: 'fast5') + ) + ch_software_versions = ch_software_versions.mix(PYCOQC.out.version.first().ifEmpty(null)) + } // // MODULE: PYCOQC, quality check for nanopore reads and Quality/Length Plots @@ -124,54 +128,43 @@ workflow BACASS { // TODO: if ( params.assembly_type == 'hybrid' || params.assembly_type == 'long' && !('short' in params.assembly_type) ) PORECHOP ( - INPUT_CHECK.out.longreads + INPUT_CHECK.out.longreads.dump(tag: 'longreads') ) ch_software_versions = ch_software_versions.mix(PORECHOP.out.version.first().ifEmpty(null)) // - // MODULE: Unicycler, nf-core module allows only short assembly assembly - // - // TODO: if ( params.assembler == 'unicycler' ) { - - UNICYCLER ( - SKEWER.out.reads - ) - ch_software_versions = ch_software_versions.mix(UNICYCLER.out.version.first().ifEmpty(null)) -/* - // - // MODULE: SPAdes, nf-core module allows only short read assembly - // - // TODO: if ( params.assembler == 'spades' ) { - // TODO: error: java.lang.NullPointerException: Cannot get property 'id' on null object - // TODO: probabaly: params.spades_hmm = false? - params.spades_hmm = false - SPADES ( - SKEWER.out.reads, params.spades_hmm - ) - ch_software_versions = ch_software_versions.mix(SPADES.out.version.first().ifEmpty(null)) -*/ - + // Join channels for assemblers. As samples have the same meta data, we can simply use join() to merge the channels based on this. If we only have one of the channels we insert 'NAs' which are not used in the unicycler process then subsequently, in case of short or long read only assembly. // - // Join channels for unicycler, as trimming the files happens in two separate processes for paralellization of individual steps. As samples have the same sampleID, we can simply use join() to merge the channels based on this. If we only have one of the channels we insert 'NAs' which are not used in the unicycler process then subsequently, in case of short or long read only assembly. - // TODO: -/* if(params.assembly_type == 'hybrid'){ + PORECHOP.out.reads.dump(tag: 'porechop') SKEWER.out.reads + .dump(tag: 'skewer') .join(PORECHOP.out.reads) .dump(tag: 'unicycler') - .set {ch_unicycler} - } else if(params.assembly_type == 'short'){ + .set { ch_for_assembly } + } else if ( params.assembly_type == 'short' ) { SKEWER.out.reads - .map{ id,R1,R2 -> tuple(id,R1,R2,'NA') } + .dump(tag: 'skewer') + .map{ meta,reads -> tuple(meta,reads,'NA') } .dump(tag: 'unicycler') - .set {ch_unicycler} - } else if(params.assembly_type == 'long'){ - ch_long_trimmed_unicycler - .map{ id,lr -> tuple(id,'NA','NA',lr) } + .set { ch_for_assembly } + } else if ( params.assembly_type == 'long' ) { + PORECHOP.out.reads + .dump(tag: 'porechop') + .map{ meta,lr -> tuple(meta,'NA',lr) } .dump(tag: 'unicycler') - .set {ch_unicycler} //old channel name: ch_short_long_joint_unicycler + .set { ch_for_assembly } //old channel name: ch_short_long_joint_unicycler + } + + // + // MODULE: Unicycler, nf-core module allows only short assembly assembly + // + if ( params.assembler == 'unicycler' ) { + UNICYCLER ( + ch_for_assembly + ) + ch_software_versions = ch_software_versions.mix(UNICYCLER.out.version.first().ifEmpty(null)) } -*/ // // MODULE: Pipeline reporting From 4034bcf0618dc831987a26e4d9087c7c30af7980 Mon Sep 17 00:00:00 2001 From: d4straub Date: Fri, 6 Aug 2021 11:25:30 +0200 Subject: [PATCH 23/81] add skewer version --- modules/local/skewer.nf | 8 ++++---- workflows/bacass.nf | 2 +- 2 files changed, 5 insertions(+), 5 deletions(-) diff --git a/modules/local/skewer.nf b/modules/local/skewer.nf index 2634806b..d2c79c7b 100644 --- a/modules/local/skewer.nf +++ b/modules/local/skewer.nf @@ -22,9 +22,9 @@ process SKEWER { tuple val(meta), path(reads) output: - tuple val(meta), path("*_trm-cmb.R{1,2}.fastq.gz") , emit: reads - path("*.log"), emit: log - //path "*.version.txt" , emit: version // TODO: output version! + tuple val(meta), path("*_trm-cmb.R{1,2}.fastq.gz"), emit: reads + path("*.log") , emit: log + path "*.version.txt" , emit: version script: def software = getSoftwareName(task.process) @@ -41,6 +41,6 @@ process SKEWER { cat \$(ls *trimmed-pair1.fastq.gz | sort) >> ${meta.id}_trm-cmb.R1.fastq.gz cat \$(ls *trimmed-pair2.fastq.gz | sort) >> ${meta.id}_trm-cmb.R2.fastq.gz - #skewer --version + echo \$(skewer --version 2>&1) | sed 's/^.*skewer version: //; s/ .*//' > ${software}.version.txt """ } diff --git a/workflows/bacass.nf b/workflows/bacass.nf index aa21118a..ff96647b 100644 --- a/workflows/bacass.nf +++ b/workflows/bacass.nf @@ -102,7 +102,7 @@ workflow BACASS { SKEWER ( INPUT_CHECK.out.shortreads.dump(tag: 'shortreads') ) - //ch_software_versions = ch_software_versions.mix(SKEWER.out.version.first().ifEmpty(null)) //TODO + ch_software_versions = ch_software_versions.mix(SKEWER.out.version.first().ifEmpty(null)) //TODO // // MODULE: Nanoplot, quality check for nanopore reads and Quality/Length Plots From b7cee694af37a31110f3fe47fd5a93c157a81196 Mon Sep 17 00:00:00 2001 From: d4straub Date: Fri, 6 Aug 2021 14:43:42 +0200 Subject: [PATCH 24/81] add prokka, kraken2 and quast --- conf/modules.config | 9 +++ modules.json | 6 ++ modules/local/prokka.nf | 50 ++++++++++++++ .../modules/kraken2/kraken2/functions.nf | 68 ++++++++++++++++++ .../nf-core/modules/kraken2/kraken2/main.nf | 53 ++++++++++++++ .../nf-core/modules/kraken2/kraken2/meta.yml | 59 ++++++++++++++++ modules/nf-core/modules/quast/functions.nf | 68 ++++++++++++++++++ modules/nf-core/modules/quast/main.nf | 48 +++++++++++++ modules/nf-core/modules/quast/meta.yml | 46 +++++++++++++ workflows/bacass.nf | 69 +++++++++++++++++-- 10 files changed, 472 insertions(+), 4 deletions(-) create mode 100644 modules/local/prokka.nf create mode 100644 modules/nf-core/modules/kraken2/kraken2/functions.nf create mode 100644 modules/nf-core/modules/kraken2/kraken2/main.nf create mode 100644 modules/nf-core/modules/kraken2/kraken2/meta.yml create mode 100644 modules/nf-core/modules/quast/functions.nf create mode 100644 modules/nf-core/modules/quast/main.nf create mode 100644 modules/nf-core/modules/quast/meta.yml diff --git a/conf/modules.config b/conf/modules.config index e133b9b8..54124e45 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -46,6 +46,15 @@ params { args = "" publish_dir = "./Unicycler" } + 'kraken2' { + args = "" + } + 'quast' { + args = "" + } + 'prokka' { + args = "" + } 'multiqc' { args = "" } diff --git a/modules.json b/modules.json index ae418c41..12cc138b 100644 --- a/modules.json +++ b/modules.json @@ -6,11 +6,17 @@ "fastqc": { "git_sha": "e937c7950af70930d1f34bb961403d9d2aa81c7d" }, + "kraken2/kraken2": { + "git_sha": "e937c7950af70930d1f34bb961403d9d2aa81c7d" + }, "multiqc": { "git_sha": "e937c7950af70930d1f34bb961403d9d2aa81c7d" }, "pycoqc": { "git_sha": "e937c7950af70930d1f34bb961403d9d2aa81c7d" + }, + "quast": { + "git_sha": "e937c7950af70930d1f34bb961403d9d2aa81c7d" } } } diff --git a/modules/local/prokka.nf b/modules/local/prokka.nf new file mode 100644 index 00000000..bf77aba8 --- /dev/null +++ b/modules/local/prokka.nf @@ -0,0 +1,50 @@ +include { initOptions; saveFiles; getSoftwareName } from './functions' + +params.options = [:] +options = initOptions(params.options) + +process PROKKA { + tag "$meta.id" + label 'process_low' + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } + + conda (params.enable_conda ? "bioconda::prokka=1.14.6" : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://depot.galaxyproject.org/singularity/prokka:1.14.6--pl526_0" + } else { + container "quay.io/biocontainers/prokka:1.14.6--pl526_0" + } + + input: + tuple val(meta), path(fasta) + + output: + tuple val(meta), path("${prefix}/*.gff"), emit: gff + tuple val(meta), path("${prefix}/*.gbk"), emit: gbk + tuple val(meta), path("${prefix}/*.fna"), emit: fna + tuple val(meta), path("${prefix}/*.faa"), emit: faa + tuple val(meta), path("${prefix}/*.ffn"), emit: ffn + tuple val(meta), path("${prefix}/*.sqn"), emit: sqn + tuple val(meta), path("${prefix}/*.fsa"), emit: fsa + tuple val(meta), path("${prefix}/*.tbl"), emit: tbl + tuple val(meta), path("${prefix}/*.err"), emit: err + tuple val(meta), path("${prefix}/*.log"), emit: log + tuple val(meta), path("${prefix}/*.txt"), emit: txt + tuple val(meta), path("${prefix}/*.tsv"), emit: tsv + path "*.version.txt", emit: version + + script: + def software = getSoftwareName(task.process) + prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" + """ + prokka \\ + $options.args \\ + --cpus $task.cpus \\ + --prefix $prefix \\ + $fasta + + echo \$(prokka --version 2>&1) | sed 's/^.*prokka //' > ${software}.version.txt + """ +} diff --git a/modules/nf-core/modules/kraken2/kraken2/functions.nf b/modules/nf-core/modules/kraken2/kraken2/functions.nf new file mode 100644 index 00000000..da9da093 --- /dev/null +++ b/modules/nf-core/modules/kraken2/kraken2/functions.nf @@ -0,0 +1,68 @@ +// +// Utility functions used in nf-core DSL2 module files +// + +// +// Extract name of software tool from process name using $task.process +// +def getSoftwareName(task_process) { + return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() +} + +// +// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules +// +def initOptions(Map args) { + def Map options = [:] + options.args = args.args ?: '' + options.args2 = args.args2 ?: '' + options.args3 = args.args3 ?: '' + options.publish_by_meta = args.publish_by_meta ?: [] + options.publish_dir = args.publish_dir ?: '' + options.publish_files = args.publish_files + options.suffix = args.suffix ?: '' + return options +} + +// +// Tidy up and join elements of a list to return a path string +// +def getPathFromList(path_list) { + def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries + paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes + return paths.join('/') +} + +// +// Function to save/publish module results +// +def saveFiles(Map args) { + if (!args.filename.endsWith('.version.txt')) { + def ioptions = initOptions(args.options) + def path_list = [ ioptions.publish_dir ?: args.publish_dir ] + if (ioptions.publish_by_meta) { + def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta + for (key in key_list) { + if (args.meta && key instanceof String) { + def path = key + if (args.meta.containsKey(key)) { + path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] + } + path = path instanceof String ? path : '' + path_list.add(path) + } + } + } + if (ioptions.publish_files instanceof Map) { + for (ext in ioptions.publish_files) { + if (args.filename.endsWith(ext.key)) { + def ext_list = path_list.collect() + ext_list.add(ext.value) + return "${getPathFromList(ext_list)}/$args.filename" + } + } + } else if (ioptions.publish_files == null) { + return "${getPathFromList(path_list)}/$args.filename" + } + } +} diff --git a/modules/nf-core/modules/kraken2/kraken2/main.nf b/modules/nf-core/modules/kraken2/kraken2/main.nf new file mode 100644 index 00000000..ea0b72fd --- /dev/null +++ b/modules/nf-core/modules/kraken2/kraken2/main.nf @@ -0,0 +1,53 @@ +// Import generic module functions +include { initOptions; saveFiles; getSoftwareName } from './functions' + +params.options = [:] +options = initOptions(params.options) + +process KRAKEN2_KRAKEN2 { + tag "$meta.id" + label 'process_high' + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } + + conda (params.enable_conda ? 'bioconda::kraken2=2.1.1 conda-forge::pigz=2.6' : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container 'https://depot.galaxyproject.org/singularity/mulled-v2-5799ab18b5fc681e75923b2450abaa969907ec98:941789bd7fe00db16531c26de8bf3c5c985242a5-0' + } else { + container 'quay.io/biocontainers/mulled-v2-5799ab18b5fc681e75923b2450abaa969907ec98:941789bd7fe00db16531c26de8bf3c5c985242a5-0' + } + + input: + tuple val(meta), path(reads) + path db + + output: + tuple val(meta), path('*classified*') , emit: classified + tuple val(meta), path('*unclassified*'), emit: unclassified + tuple val(meta), path('*report.txt') , emit: txt + path '*.version.txt' , emit: version + + script: + def software = getSoftwareName(task.process) + def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" + def paired = meta.single_end ? "" : "--paired" + def classified = meta.single_end ? "${prefix}.classified.fastq" : "${prefix}.classified#.fastq" + def unclassified = meta.single_end ? "${prefix}.unclassified.fastq" : "${prefix}.unclassified#.fastq" + """ + kraken2 \\ + --db $db \\ + --threads $task.cpus \\ + --unclassified-out $unclassified \\ + --classified-out $classified \\ + --report ${prefix}.kraken2.report.txt \\ + --gzip-compressed \\ + $paired \\ + $options.args \\ + $reads + + pigz -p $task.cpus *.fastq + + echo \$(kraken2 --version 2>&1) | sed 's/^.*Kraken version //; s/ .*\$//' > ${software}.version.txt + """ +} diff --git a/modules/nf-core/modules/kraken2/kraken2/meta.yml b/modules/nf-core/modules/kraken2/kraken2/meta.yml new file mode 100644 index 00000000..cb1ec0de --- /dev/null +++ b/modules/nf-core/modules/kraken2/kraken2/meta.yml @@ -0,0 +1,59 @@ +name: kraken2_kraken2 +description: Classifies metagenomic sequence data +keywords: + - classify + - metagenomics + - fastq + - db +tools: + - kraken2: + description: | + Kraken2 is a taxonomic sequence classifier that assigns taxonomic labels to sequence reads + homepage: https://ccb.jhu.edu/software/kraken2/ + documentation: https://github.com/DerrickWood/kraken2/wiki/Manual + doi: 10.1186/s13059-019-1891-0 +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - reads: + type: file + description: | + List of input FastQ files of size 1 and 2 for single-end and paired-end data, + respectively. + - db: + type: directory + description: Kraken2 database +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - classified: + type: file + description: | + Reads classified to belong to any of the taxa + on the Kraken2 database. + pattern: "*{fastq.gz}" + - unclassified: + type: file + description: | + Reads not classified to belong to any of the taxa + on the Kraken2 database. + pattern: "*{fastq.gz}" + - txt: + type: file + description: | + Kraken2 report containing stats about classified + and not classifed reads. + pattern: "*.{report.txt}" + - version: + type: file + description: File containing software version + pattern: "*.{version.txt}" +authors: + - "@joseespinosa" + - "@drpatelh" diff --git a/modules/nf-core/modules/quast/functions.nf b/modules/nf-core/modules/quast/functions.nf new file mode 100644 index 00000000..da9da093 --- /dev/null +++ b/modules/nf-core/modules/quast/functions.nf @@ -0,0 +1,68 @@ +// +// Utility functions used in nf-core DSL2 module files +// + +// +// Extract name of software tool from process name using $task.process +// +def getSoftwareName(task_process) { + return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() +} + +// +// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules +// +def initOptions(Map args) { + def Map options = [:] + options.args = args.args ?: '' + options.args2 = args.args2 ?: '' + options.args3 = args.args3 ?: '' + options.publish_by_meta = args.publish_by_meta ?: [] + options.publish_dir = args.publish_dir ?: '' + options.publish_files = args.publish_files + options.suffix = args.suffix ?: '' + return options +} + +// +// Tidy up and join elements of a list to return a path string +// +def getPathFromList(path_list) { + def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries + paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes + return paths.join('/') +} + +// +// Function to save/publish module results +// +def saveFiles(Map args) { + if (!args.filename.endsWith('.version.txt')) { + def ioptions = initOptions(args.options) + def path_list = [ ioptions.publish_dir ?: args.publish_dir ] + if (ioptions.publish_by_meta) { + def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta + for (key in key_list) { + if (args.meta && key instanceof String) { + def path = key + if (args.meta.containsKey(key)) { + path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] + } + path = path instanceof String ? path : '' + path_list.add(path) + } + } + } + if (ioptions.publish_files instanceof Map) { + for (ext in ioptions.publish_files) { + if (args.filename.endsWith(ext.key)) { + def ext_list = path_list.collect() + ext_list.add(ext.value) + return "${getPathFromList(ext_list)}/$args.filename" + } + } + } else if (ioptions.publish_files == null) { + return "${getPathFromList(path_list)}/$args.filename" + } + } +} diff --git a/modules/nf-core/modules/quast/main.nf b/modules/nf-core/modules/quast/main.nf new file mode 100644 index 00000000..0b94c410 --- /dev/null +++ b/modules/nf-core/modules/quast/main.nf @@ -0,0 +1,48 @@ +// Import generic module functions +include { initOptions; saveFiles; getSoftwareName } from './functions' + +params.options = [:] +options = initOptions(params.options) + +process QUAST { + label 'process_medium' + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:[:], publish_by_meta:[]) } + + conda (params.enable_conda ? 'bioconda::quast=5.0.2' : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container 'https://depot.galaxyproject.org/singularity/quast:5.0.2--py37pl526hb5aa323_2' + } else { + container 'quay.io/biocontainers/quast:5.0.2--py37pl526hb5aa323_2' + } + + input: + path consensus + path fasta + path gff + val use_fasta + val use_gff + + output: + path "${prefix}" , emit: results + path '*.tsv' , emit: tsv + path '*.version.txt', emit: version + + script: + def software = getSoftwareName(task.process) + prefix = options.suffix ?: software + def features = use_gff ? "--features $gff" : '' + def reference = use_fasta ? "-r $fasta" : '' + """ + quast.py \\ + --output-dir $prefix \\ + $reference \\ + $features \\ + --threads $task.cpus \\ + $options.args \\ + ${consensus.join(' ')} + ln -s ${prefix}/report.tsv + echo \$(quast.py --version 2>&1) | sed 's/^.*QUAST v//; s/ .*\$//' > ${software}.version.txt + """ +} diff --git a/modules/nf-core/modules/quast/meta.yml b/modules/nf-core/modules/quast/meta.yml new file mode 100644 index 00000000..cc79486e --- /dev/null +++ b/modules/nf-core/modules/quast/meta.yml @@ -0,0 +1,46 @@ +name: quast +description: Quality Assessment Tool for Genome Assemblies +keywords: + - quast + - assembly + - quality +tools: + - quast: + description: | + QUAST calculates quality metrics for genome assemblies + homepage: http://bioinf.spbau.ru/quast + doi: +input: + - consensus: + type: file + description: | + Fasta file containing the assembly of interest + - fasta: + type: file + description: | + The genome assembly to be evaluated. Has to contain at least a non-empty string dummy value. + - use_fasta: + type: boolean + description: Whether to use the provided fasta reference genome file + - gff: + type: file + description: The genome GFF file. Has to contain at least a non-empty string dummy value. + - use_gff: + type: boolean + description: Whether to use the provided gff reference annotation file + +output: + - quast: + type: directory + description: Directory containing complete quast report + pattern: "{prefix}.lineage_report.csv" + - report: + + - version: + type: file + description: File containing software version + pattern: "*.{version.txt}" + +authors: + - "@drpatelh" + - "@kevinmenden" diff --git a/workflows/bacass.nf b/workflows/bacass.nf index ff96647b..74034119 100644 --- a/workflows/bacass.nf +++ b/workflows/bacass.nf @@ -17,6 +17,15 @@ for (param in checkPathParamList) { if (param) { file(param, checkIfExists: true // Check mandatory parameters if (params.input) { ch_input = file(params.input) } else { exit 1, 'Input samplesheet not specified!' } +// Check krakendb +if(! params.skip_kraken2){ + if(params.kraken2db){ + kraken2db = file(params.kraken2db) + } else { + exit 1, "Missing Kraken2 DB arg" + } +} + /* ======================================================================================== CONFIG FILES @@ -38,6 +47,9 @@ def modules = params.modules.clone() def unicycler_options = modules['unicycler'] unicycler_options.args += " $params.unicycler_args" +def prokka_options = modules['prokka'] +prokka_options.args += " $params.prokka_args" + // // MODULE: Local to the pipeline // @@ -46,6 +58,7 @@ include { SKEWER } from '../modules/local/skewer' include { NANOPLOT } from '../modules/local/nanoplot' addParams( options: modules['nanoplot'] ) include { PORECHOP } from '../modules/local/porechop' addParams( options: modules['porechop'] ) include { UNICYCLER } from '../modules/local/unicycler' addParams( options: unicycler_options ) +include { PROKKA } from '../modules/local/prokka' addParams( options: prokka_options ) // // SUBWORKFLOW: Consisting of a mix of local and nf-core/modules @@ -64,9 +77,11 @@ multiqc_options.args += params.multiqc_title ? Utils.joinModuleArgs(["--title \" // // MODULE: Installed directly from nf-core/modules // -include { FASTQC } from '../modules/nf-core/modules/fastqc/main' addParams( options: modules['fastqc'] ) -include { PYCOQC } from '../modules/nf-core/modules/pycoqc/main' addParams( options: modules['pycoqc'] ) -include { MULTIQC } from '../modules/nf-core/modules/multiqc/main' addParams( options: multiqc_options ) +include { FASTQC } from '../modules/nf-core/modules/fastqc/main' addParams( options: modules['fastqc'] ) +include { PYCOQC } from '../modules/nf-core/modules/pycoqc/main' addParams( options: modules['pycoqc'] ) +include { KRAKEN2_KRAKEN2 as KRAKEN2 } from '../modules/nf-core/modules/kraken2/kraken2/main' addParams( options: modules['kraken2'] ) +include { QUAST } from '../modules/nf-core/modules/quast/main' addParams( options: modules['quast'] ) +include { MULTIQC } from '../modules/nf-core/modules/multiqc/main' addParams( options: multiqc_options ) /* ======================================================================================== @@ -157,7 +172,7 @@ workflow BACASS { } // - // MODULE: Unicycler, nf-core module allows only short assembly assembly + // MODULE: Unicycler, genome assembly, nf-core module allows only short assembly // if ( params.assembler == 'unicycler' ) { UNICYCLER ( @@ -166,6 +181,52 @@ workflow BACASS { ch_software_versions = ch_software_versions.mix(UNICYCLER.out.version.first().ifEmpty(null)) } + // + // MODULE: Kraken2, QC for sample purity + // + if ( !params.skip_kraken2 ) { + //prepare channel that specifies "meta.single_end = true" for long reads + PORECHOP.out.reads + .map { info, reads -> + def meta = info + meta.single_end = true + [ meta, reads ] } + .mix(SKEWER.out.reads) + .set { ch_for_kraken2 } + KRAKEN2 ( + ch_for_kraken2.dump(tag: 'kraken2'), + kraken2db + ) + ch_software_versions = ch_software_versions.mix(KRAKEN2.out.version.first().ifEmpty(null)) + } + + // + // MODULE: QUAST, assembly QC + // + UNICYCLER.out.scaffolds + .dump(tag: 'unicycler') + .map { meta, fasta -> fasta } + .collect() + .set { ch_to_quast } + QUAST ( + ch_to_quast, + file('fasta'), + file('gff'), + false, + false + ) + ch_software_versions = ch_software_versions.mix(QUAST.out.version.ifEmpty(null)) + + // + // MODULE: PROKKA, gene annotation + // + if ( !params.skip_annotation && params.annotation_tool == 'prokka' ) { + PROKKA ( + UNICYCLER.out.scaffolds + ) + ch_software_versions = ch_software_versions.mix(PROKKA.out.version.first().ifEmpty(null)) + } + // // MODULE: Pipeline reporting // From 6c8997649bffd1822206602e4d750ac5afbc6e99 Mon Sep 17 00:00:00 2001 From: d4straub Date: Fri, 6 Aug 2021 14:46:12 +0200 Subject: [PATCH 25/81] fix linting --- workflows/bacass.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/workflows/bacass.nf b/workflows/bacass.nf index 74034119..4a383040 100644 --- a/workflows/bacass.nf +++ b/workflows/bacass.nf @@ -102,7 +102,7 @@ workflow BACASS { INPUT_CHECK ( ch_input ) - + // // MODULE: Run FastQC // From 90392bdec95b274856c333352eac7c10b10ae3c1 Mon Sep 17 00:00:00 2001 From: d4straub Date: Fri, 6 Aug 2021 15:01:32 +0200 Subject: [PATCH 26/81] samplesheet from tsv to csv again --- nextflow_schema.json | 2 +- subworkflows/local/input_check.nf | 38 +++++++++++++++---------------- 2 files changed, 20 insertions(+), 20 deletions(-) diff --git a/nextflow_schema.json b/nextflow_schema.json index 71156fa6..cf597abc 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -21,7 +21,7 @@ "pattern": "^\\S+\\.csv$", "schema": "assets/schema_input.json", "description": "Path to comma-separated file containing information about the samples in the experiment.", - "help_text": "You will need to create a design file with information about the samples in your experiment before running the pipeline. Use this parameter to specify its location. It has to be a comma-separated file with 6 columns, and a header row. See [usage docs](https://nf-co.re/bacass/usage#samplesheet-input).\n\nFor example:\n\n`--input 'design_hybrid.csv'`\n\nAn example of properly formatted input files can be found at the [nf-core/testData](https://github.com/nf-core/test-datasets/tree/bacass). \n\nExemplarily, this is the input used for a hybrid assembly in testing:\nID,R1,R2,long_fastq,fast5,genome_size\nERR044595,ERR044595_1M_1.fastq.gz,ERR044595_1M_2.fastq.gz,subset15000.fq.gz,NA,2.8m\n\n* `ID`: The identifier to use for handling the dataset e.g. sample name\n* `R1`: The forward reads in case of available short-read data\n* `R2`: The reverse reads in case of available short-read data\n* `long_fastq`: The long read FastQ file with reads in FASTQ format\n* `fast5`: The folder containing the basecalled fast5 files\n* `genome_size`: The expected genome size of the assembly. Only used by the canu assembler.\n\nMissing values (e.g. fast5 folder in case of short reads) can be omitted by using a `NA` in the CSV file. The pipeline will handle such cases appropriately then.", + "help_text": "You will need to create a design file with information about the samples in your experiment before running the pipeline. Use this parameter to specify its location. It has to be a tab-separated file with 6 columns, and a header row. See [usage docs](https://nf-co.re/bacass/usage#samplesheet-input).\n\nFor example:\n\n`--input 'design_hybrid.csv'`\n\nAn example of properly formatted input files can be found at the [nf-core/testData](https://github.com/nf-core/test-datasets/tree/bacass). \n\nExemplarily, this is the input used for a hybrid assembly in testing:\nID R1 R2 LongFastQ Fast5 GenomeSize\nERR044595 https://github.com/nf-core/test-datasets/raw/bacass/ERR044595_1M_1.fastq.gz https://github.com/nf-core/test-datasets/raw/bacass/ERR044595_1M_2.fastq.gz https://github.com/nf-core/test-datasets/raw/bacass/nanopore/subset15000.fq.gz NA 2.8m\n\n* `ID`: The identifier to use for handling the dataset e.g. sample name\n* `R1`: The forward reads in case of available short-read data\n* `R2`: The reverse reads in case of available short-read data\n* `LongFastQ`: The long read FastQ file with reads in FASTQ format\n* `Fast5`: The folder containing the basecalled fast5 files\n* `GenomeSize`: The expected genome size of the assembly. Only used by the canu assembler.\n\nMissing values (e.g. Fast5 folder in case of short reads) can be omitted by using a `NA` in the TSV file. The pipeline will handle such cases appropriately then.", "fa_icon": "fas fa-file-csv" }, "outdir": { diff --git a/subworkflows/local/input_check.nf b/subworkflows/local/input_check.nf index 043578bb..dfb05ecb 100644 --- a/subworkflows/local/input_check.nf +++ b/subworkflows/local/input_check.nf @@ -14,7 +14,7 @@ workflow INPUT_CHECK { Channel .fromPath( samplesheet ) .ifEmpty {exit 1, log.info "Cannot find path file ${tsvFile}"} - .splitCsv ( header:true, sep:',' ) + .splitCsv ( header:true, sep:'\t' ) .map { create_fastq_channels(it) } .set { reads } @@ -43,39 +43,39 @@ workflow INPUT_CHECK { // Function to get list of [ meta, [ fastq_1, fastq_2 ], long_fastq, fast5 ] def create_fastq_channels(LinkedHashMap row) { def meta = [:] - meta.id = row.sample + meta.id = row.ID meta.single_end = false - meta.genome_size = row.genome_size == null ? 'NA' : row.genome_size + meta.genome_size = row.GenomeSize == null ? 'NA' : row.GenomeSize def array = [] // check short reads - if ( !(row.fastq_1 == 'NA') ) { - if ( !file(row.fastq_1).exists() ) { - exit 1, "ERROR: Please check input samplesheet -> Read 1 FastQ file does not exist!\n${row.fastq_1}" + if ( !(row.R1 == 'NA') ) { + if ( !file(row.R1).exists() ) { + exit 1, "ERROR: Please check input samplesheet -> Read 1 FastQ file does not exist!\n${row.R1}" } - fastq_1 = file(row.fastq_1) + fastq_1 = file(row.R1) } else { fastq_1 = 'NA' } - if ( !(row.fastq_2 == 'NA') ) { - if ( !file(row.fastq_2).exists() ) { - exit 1, "ERROR: Please check input samplesheet -> Read 2 FastQ file does not exist!\n${row.fastq_2}" + if ( !(row.R2 == 'NA') ) { + if ( !file(row.R2).exists() ) { + exit 1, "ERROR: Please check input samplesheet -> Read 2 FastQ file does not exist!\n${row.R2}" } - fastq_2 = file(row.fastq_2) + fastq_2 = file(row.R2) } else { fastq_2 = 'NA' } // check long_fastq - if ( !(row.long_fastq == 'NA') ) { - if ( !file(row.long_fastq).exists() ) { - exit 1, "ERROR: Please check input samplesheet -> Long FastQ file does not exist!\n${row.fastq_1}" + if ( !(row.LongFastQ == 'NA') ) { + if ( !file(row.LongFastQ).exists() ) { + exit 1, "ERROR: Please check input samplesheet -> Long FastQ file does not exist!\n${row.R1}" } - long_fastq = file(row.long_fastq) + long_fastq = file(row.LongFastQ) } else { long_fastq = 'NA' } // check long_fastq - if ( !(row.fast5 == 'NA') ) { - if ( !file(row.fast5).exists() ) { - exit 1, "ERROR: Please check input samplesheet -> Fast5 file does not exist!\n${row.fastq_1}" + if ( !(row.Fast5 == 'NA') ) { + if ( !file(row.Fast5).exists() ) { + exit 1, "ERROR: Please check input samplesheet -> Fast5 file does not exist!\n${row.R1}" } - fast5 = file(row.fast5) + fast5 = file(row.Fast5) } else { fast5 = 'NA' } // prepare output // currently does not allow single end data! From 8f721031882ee76da4f22eee390ee5e0c9afadac Mon Sep 17 00:00:00 2001 From: d4straub Date: Fri, 6 Aug 2021 15:21:08 +0200 Subject: [PATCH 27/81] use nf-core prokka module --- modules.json | 3 + modules/nf-core/modules/prokka/functions.nf | 68 ++++++++++++++ .../modules/prokka/main.nf} | 6 ++ modules/nf-core/modules/prokka/meta.yml | 91 +++++++++++++++++++ workflows/bacass.nf | 12 ++- 5 files changed, 175 insertions(+), 5 deletions(-) create mode 100644 modules/nf-core/modules/prokka/functions.nf rename modules/{local/prokka.nf => nf-core/modules/prokka/main.nf} (89%) create mode 100644 modules/nf-core/modules/prokka/meta.yml diff --git a/modules.json b/modules.json index 12cc138b..f81715a1 100644 --- a/modules.json +++ b/modules.json @@ -12,6 +12,9 @@ "multiqc": { "git_sha": "e937c7950af70930d1f34bb961403d9d2aa81c7d" }, + "prokka": { + "git_sha": "e937c7950af70930d1f34bb961403d9d2aa81c7d" + }, "pycoqc": { "git_sha": "e937c7950af70930d1f34bb961403d9d2aa81c7d" }, diff --git a/modules/nf-core/modules/prokka/functions.nf b/modules/nf-core/modules/prokka/functions.nf new file mode 100644 index 00000000..da9da093 --- /dev/null +++ b/modules/nf-core/modules/prokka/functions.nf @@ -0,0 +1,68 @@ +// +// Utility functions used in nf-core DSL2 module files +// + +// +// Extract name of software tool from process name using $task.process +// +def getSoftwareName(task_process) { + return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() +} + +// +// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules +// +def initOptions(Map args) { + def Map options = [:] + options.args = args.args ?: '' + options.args2 = args.args2 ?: '' + options.args3 = args.args3 ?: '' + options.publish_by_meta = args.publish_by_meta ?: [] + options.publish_dir = args.publish_dir ?: '' + options.publish_files = args.publish_files + options.suffix = args.suffix ?: '' + return options +} + +// +// Tidy up and join elements of a list to return a path string +// +def getPathFromList(path_list) { + def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries + paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes + return paths.join('/') +} + +// +// Function to save/publish module results +// +def saveFiles(Map args) { + if (!args.filename.endsWith('.version.txt')) { + def ioptions = initOptions(args.options) + def path_list = [ ioptions.publish_dir ?: args.publish_dir ] + if (ioptions.publish_by_meta) { + def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta + for (key in key_list) { + if (args.meta && key instanceof String) { + def path = key + if (args.meta.containsKey(key)) { + path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] + } + path = path instanceof String ? path : '' + path_list.add(path) + } + } + } + if (ioptions.publish_files instanceof Map) { + for (ext in ioptions.publish_files) { + if (args.filename.endsWith(ext.key)) { + def ext_list = path_list.collect() + ext_list.add(ext.value) + return "${getPathFromList(ext_list)}/$args.filename" + } + } + } else if (ioptions.publish_files == null) { + return "${getPathFromList(path_list)}/$args.filename" + } + } +} diff --git a/modules/local/prokka.nf b/modules/nf-core/modules/prokka/main.nf similarity index 89% rename from modules/local/prokka.nf rename to modules/nf-core/modules/prokka/main.nf index bf77aba8..1fa3f3d9 100644 --- a/modules/local/prokka.nf +++ b/modules/nf-core/modules/prokka/main.nf @@ -19,6 +19,8 @@ process PROKKA { input: tuple val(meta), path(fasta) + path proteins + path prodigal_tf output: tuple val(meta), path("${prefix}/*.gff"), emit: gff @@ -38,11 +40,15 @@ process PROKKA { script: def software = getSoftwareName(task.process) prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" + def proteins_opt = proteins ? "--proteins ${proteins[0]}" : "" + def prodigal_opt = prodigal_tf ? "--prodigaltf ${prodigal_tf[0]}" : "" """ prokka \\ $options.args \\ --cpus $task.cpus \\ --prefix $prefix \\ + $proteins_opt \\ + $prodigal_tf \\ $fasta echo \$(prokka --version 2>&1) | sed 's/^.*prokka //' > ${software}.version.txt diff --git a/modules/nf-core/modules/prokka/meta.yml b/modules/nf-core/modules/prokka/meta.yml new file mode 100644 index 00000000..4489b2fd --- /dev/null +++ b/modules/nf-core/modules/prokka/meta.yml @@ -0,0 +1,91 @@ +name: prokka +description: Whole genome annotation of small genomes (bacterial, archeal, viral) +keywords: + - annotation + - fasta + - prokka +tools: + - prokka: + description: Rapid annotation of prokaryotic genomes + homepage: https://github.com/tseemann/prokka + doi: "10.1093/bioinformatics/btu153" + licence: ['GPL v2'] + +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - fasta: + type: file + description: | + FASTA file to be annotated. Has to contain at least a non-empty string dummy value. + - proteins: + type: file + description: FASTA file of trusted proteins to first annotate from (optional) + - prodigal_tf: + type: file + description: Training file to use for Prodigal (optional) + +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - version: + type: file + description: File containing software version + pattern: "*.{version.txt}" + - gff: + type: file + description: annotation in GFF3 format, containing both sequences and annotations + pattern: "*.{gff}" + - gbk: + type: file + description: annotation in GenBank format, containing both sequences and annotations + pattern: "*.{gbk}" + - fna: + type: file + description: nucleotide FASTA file of the input contig sequences + pattern: "*.{fna}" + - faa: + type: file + description: protein FASTA file of the translated CDS sequences + pattern: "*.{faa}" + - ffn: + type: file + description: nucleotide FASTA file of all the prediction transcripts (CDS, rRNA, tRNA, tmRNA, misc_RNA) + pattern: "*.{ffn}" + - sqn: + type: file + description: an ASN1 format "Sequin" file for submission to Genbank + pattern: "*.{sqn}" + - fsa: + type: file + description: nucleotide FASTA file of the input contig sequences, used by "tbl2asn" to create the .sqn file + pattern: "*.{fsa}" + - tbl: + type: file + description: feature Table file, used by "tbl2asn" to create the .sqn file + pattern: "*.{tbl}" + - err: + type: file + description: unacceptable annotations - the NCBI discrepancy report. + pattern: "*.{err}" + - log: + type: file + description: contains all the output that Prokka produced during its run + pattern: "*.{log}" + - txt: + type: file + description: statistics relating to the annotated features found + pattern: "*.{txt}" + - tsv: + type: file + description: tab-separated file of all features (locus_tag,ftype,len_bp,gene,EC_number,COG,product) + pattern: "*.{tsv}" + +authors: + - "@rpetit3" diff --git a/workflows/bacass.nf b/workflows/bacass.nf index 4a383040..cbf7d5a3 100644 --- a/workflows/bacass.nf +++ b/workflows/bacass.nf @@ -47,9 +47,6 @@ def modules = params.modules.clone() def unicycler_options = modules['unicycler'] unicycler_options.args += " $params.unicycler_args" -def prokka_options = modules['prokka'] -prokka_options.args += " $params.prokka_args" - // // MODULE: Local to the pipeline // @@ -58,7 +55,6 @@ include { SKEWER } from '../modules/local/skewer' include { NANOPLOT } from '../modules/local/nanoplot' addParams( options: modules['nanoplot'] ) include { PORECHOP } from '../modules/local/porechop' addParams( options: modules['porechop'] ) include { UNICYCLER } from '../modules/local/unicycler' addParams( options: unicycler_options ) -include { PROKKA } from '../modules/local/prokka' addParams( options: prokka_options ) // // SUBWORKFLOW: Consisting of a mix of local and nf-core/modules @@ -74,6 +70,9 @@ include { INPUT_CHECK } from '../subworkflows/local/input_check' addParams( opti def multiqc_options = modules['multiqc'] multiqc_options.args += params.multiqc_title ? Utils.joinModuleArgs(["--title \"$params.multiqc_title\""]) : '' +def prokka_options = modules['prokka'] +prokka_options.args += " $params.prokka_args" + // // MODULE: Installed directly from nf-core/modules // @@ -81,6 +80,7 @@ include { FASTQC } from '../modules/nf-core/modules/fastqc/main' add include { PYCOQC } from '../modules/nf-core/modules/pycoqc/main' addParams( options: modules['pycoqc'] ) include { KRAKEN2_KRAKEN2 as KRAKEN2 } from '../modules/nf-core/modules/kraken2/kraken2/main' addParams( options: modules['kraken2'] ) include { QUAST } from '../modules/nf-core/modules/quast/main' addParams( options: modules['quast'] ) +include { PROKKA } from '../modules/nf-core/modules/prokka/main' addParams( options: prokka_options ) include { MULTIQC } from '../modules/nf-core/modules/multiqc/main' addParams( options: multiqc_options ) /* @@ -222,7 +222,9 @@ workflow BACASS { // if ( !params.skip_annotation && params.annotation_tool == 'prokka' ) { PROKKA ( - UNICYCLER.out.scaffolds + UNICYCLER.out.scaffolds, + [], + [] ) ch_software_versions = ch_software_versions.mix(PROKKA.out.version.first().ifEmpty(null)) } From f72bc4147d5814827cbbf4f34ee5dd2878692f17 Mon Sep 17 00:00:00 2001 From: d4straub Date: Fri, 6 Aug 2021 16:37:30 +0200 Subject: [PATCH 28/81] add dfast --- assets/test_config_dfast.py | 284 ++++++++++++++++++++++++++++++++++++ conf/modules.config | 3 + conf/test_dfast.config | 1 + modules/local/dfast.nf | 36 +++++ nextflow.config | 10 +- workflows/bacass.nf | 13 ++ 6 files changed, 344 insertions(+), 3 deletions(-) create mode 100644 assets/test_config_dfast.py create mode 100644 modules/local/dfast.nf diff --git a/assets/test_config_dfast.py b/assets/test_config_dfast.py new file mode 100644 index 00000000..234b0bdc --- /dev/null +++ b/assets/test_config_dfast.py @@ -0,0 +1,284 @@ +#! /usr/bin/env python +# coding:utf8 +# Imported from DFAST repository to provide default config file +import multiprocessing + + +class Config: + GENOME_FASTA = "@@APP_ROOT@@/example/test.genome.fna" + WORK_DIR = "RESULT_TEST" + + CPU = 1 + FORCE_OVERWRITE = True + DEBUG = False + + GENOME_CONFIG = { + # Set 'complete' to True to annotate a complete genome. + # If 'use_original_name' is set to True, the leading non-space letters in the Fasta header line will be used as a sequence name. + # Otherwise, sequences will be renamed as 'Sequence001, Sequence002, ...'. + # If 'sort_by_length' is set to True, sequences will be sorted so that longer sequences come first. + # In a draft genome, sequences shorter than 'minimum_length' will be eliminated. + + "complete": False, + "use_original_name": False, # If set to True, the first word in the Fasta header line will be used as a sequence name. + "sort_by_length": True, + "minimum_length": 200 + } + + GENOME_SOURCE_INFORMATION = { + # These attributes are reflected in the source feature, + # and do not affect the annotation results. + + "organism": "", + "strain": "", + "seq_names": "", + "seq_types": "", + "seq_topologies": "", + "additional_modifiers": "", + } + + LOCUS_TAG_SETTINGS = { + "locus_tag_prefix": "LOCUS", + "step": 10, + "use_separate_tags": True, # If set to `True`, locus_tags are assigned separately according to feature type. + "symbols": {"CDS": "", "rRNA": "r", "tRNA": "t", "tmRNA": "tm"} + # "symbols": {"CDS": "", "rRNA": "r", "tRNA": "t", "tmRNA": "tm", "nc_rna": "nc", "misc_rna": "misc"} + } + + FEATURE_ADJUSTMENT = { + "remove_partial_features": True, # True: enabled, False: disabled + + "remove_overlapping_features": True, # True: enabled, False: disabled + "feature_type_priority": ["assembly_gap", "CRISPR", ("tmRNA", "tRNA", "rRNA"), "CDS"], + + "merge_cds": False, # True: enabled, False: disabled + "tool_type_priority": {"MGA": 0, "Prodigal": 1}, + } + + OUTPUT_RESULT = { + # output verbosity level for .gff and .gbk + "verbosity": 3 # 1: minimum, 2: standard, 3: rich + } + + DDBJ_SUBMISSION = { + "enabled": True, + "output_verbosity": 1, + "metadata_file": None + } + + GENBANK_SUBMISSION = { + "enabled": True, + "center_name": "", # Genome Center tag for GenBank submission + "output_verbosity": 1, + } + + + STRUCTURAL_ANNOTATION = [ + { + # GAP is a Gap Annotation Process that identifies gap regions (N's or n's runs) in the sequence. + "tool_name": "GAP", + "enabled": True, + "options": { + "len_cutoff": 5, # Gaps shorter than len_cutoff are ignored. + "linkage_evidence": "paired-ends", # You can change this as you like. + "gap_type": "within scaffold" # You can change this as you like. + }, + }, + { + # MetaGeneAnnotator for CDS prediction + "tool_name": "MGA", + "enabled": True, + "options": {"cmd_options": "-s"}, # -s for single species, -m for multiple species + }, + { + # Aragorn for tRNA and tmRNA prediction + "tool_name": "Aragorn", + "enabled": False, + "options": { + "gcode": "-gcbact", # -gcbact for Bacterial/Plant Chloroplast genetic code, "-gcstd" for standard genetic code. + "cmd_options": "-l", # DFAST assumes linear sequences as input. + }, + }, + { + # tRNAscan-SE for tRNA prediction. By default, this is disabled. + # Please insall tRNAscan-SE and put it in your PATH to enable this. + "tool_name": "tRNAscan", + "enabled": False, + "options": { + "model": "--bact", # --bact, --arch, --organ, --general + "cmd_options": "" + }, + }, + { + # Barrnap for rRNA prediction + "tool_name": "Barrnap", + "enabled": False, + "options": { + # Currently, Barrnap will run with default settings. + # You can set parameters such as --reject and --lencutoff to cmd_options. + # "cmd_options": "--reject 0.4 --lencutoff 0.6" + }, + }, + { + # RNAmmer for rRNA prediction. By default, this is disabled. + # Please insall RNAmmer and put it in your PATH to enable this. + "tool_name": "RNAmmer", + "enabled": False, + "options": { + "model": "bac", # arc/bac/euk + "cmd_options": "" + }, + }, + { + # CRT for CRISPR detection + # CRT runs on JavaVM. Depending on your system, it requires additional parameters to run. + # Example + # "java_options": "-Xmx256m" + "tool_name": "CRT", + "enabled": False, + "options": { + "jar_file": "@@APP_ROOT@@/bin/common/CRT1.2-CLI.jar", + "java_options": "", # options passed to java. e.g. -Xmx256m + "cmd_options": "", # cmd_options passed to CRT. + }, + }, + { + # Prodigal for CDS prediction + # By default Prodigal is disabled. To enable this, also set MGA disabled or enable merge_cds in FEATURE_ADJUSTMENT. + "tool_name": "Prodigal", + "enabled": False, + "options": { + "transl_table": 11, + "cmd_options": "", + }, + }, + ] + + FUNCTIONAL_ANNOTATION = [ + # Fucntional annotation steps will be conducted in the order specified in this list. + # You can swithch enabled/disabled, change the order, or add new steps. + + { + # OrthoSearch (All-vs-all pairwise alignment between each reference genome to assign orthologous genes) + # Normally, this should be run before other annotation steps. + # In the default workflow, it is disabled. You can enable this by using the "--references" option. + "component_name": "OrthoSearch", + "enabled": False, + "options": { + "skipAnnotatedFeatures": False, + "evalue_cutoff": 1e-6, + "qcov_cutoff": 75, + "scov_cutoff": 75, + "aligner": "ghostx", # ghostx, ghostz, or blastp + "aligner_options": {}, # Normally, leave this empty. (Current version does not use this option.) + "references": [ + ] + }, + }, + { + # By default, this is disabled. + # If you want to add your original databases to be searched in prior to default DB, + # set 'enabled' to True and specify 'database' + # The database file must be in a DFAST reference format, + # and DB indexing must be done using the 'reference_util.py' script. + "component_name": "DBsearch", + "enabled": False, + "options": { + "skipAnnotatedFeatures": True, + "evalue_cutoff": 1e-6, + "qcov_cutoff": 75, + "scov_cutoff": 75, + "aligner": "ghostx", # ghostx, ghostz or blastp + "aligner_options": {}, # Normally, leave this empty. (Current version does not use this option.) + "database": "", + }, + }, + { + # Sequence homology search against the default DB. + "component_name": "DBsearch", + "enabled": True, + "options": { + "skipAnnotatedFeatures": True, + "evalue_cutoff": 1e-6, + "qcov_cutoff": 75, + "scov_cutoff": 75, + "aligner": "ghostx", # ghostz, ghostx or blastp + "aligner_options": {}, # Normally, leave this empty. (Current version does not use this option.) + "database": "./protein/DFAST-default.ref", + }, + }, + { + # This is for homology search against a large-sized reference database, + # such as pre-formatted Blast databases like RefSeq Protein. + "component_name": "BlastSearch", + "enabled": False, + "options": { + "skipAnnotatedFeatures": False, + "evalue_cutoff": 1e-6, + "qcov_cutoff": 75, + "scov_cutoff": 75, + "aligner": "blastp", # must be blastp + "aligner_options": {}, # Normally, leave this empty. (Current version does not use this option.) + "dbtype": "auto", # Must be either of auto/ncbi/uniprot/plain + "database": "", + }, + }, + { + # Pseudo/frame-shifted genes detection based on the upstream homology search results. + # This also detects translation exceptions to selenocystein/pyrrolysine. + "component_name": "PseudoGeneDetection", + "enabled": True, + "options": { + "skipAnnotatedFeatures": False, + "extension": 300, + "scov_cutoff": 85, + }, + }, + { + # Search against a profile-HMM database using hmmscan. + # In the standard workflow, TIGRFAM HMM library will be searched. + "component_name": "HMMscan", + "enabled": False, + "options": { + "skipAnnotatedFeatures": True, + "evalue_cutoff": 1e-6, + "database": "@@APP_ROOT@@/db/hmm/TIGRFAMs_15.0_HMM.LIB", + "db_name": "TIGR", + }, + }, + { + # You can add another HMMscan, if you want. + "component_name": "HMMscan", + "enabled": False, + "options": { + "skipAnnotatedFeatures": False, + "evalue_cutoff": 1e-6, + "db_name": "", # eg 'Pfam', + "database": "" # eg '@@APP_ROOT@@/db/hmm/Pfam-A.hmm' + }, + }, + { + # Search against Conserved Domain Database (CDD) using RPS-BLAST + # In the standard workflow, COG functional categories will be assigned by this step. + "component_name": "CDDsearch", + "enabled": False, + "options": { + "skipAnnotatedFeatures": False, + "evalue_cutoff": 1e-6, + "database": "@@APP_ROOT@@/db/cdd/Cog", + "rpsbproc_data": "@@APP_ROOT@@/bin/common/rpsbproc_data", # Do not change this. + }, + }, + { + # You can add another CDDsearch, if you want. + "component_name": "CDDsearch", + "enabled": False, + "options": { + "skipAnnotatedFeatures": False, + "evalue_cutoff": 1e-6, + "database": "", # eg @@APP_ROOT@@/db/cdd/Prk + "rpsbproc_data": "@@APP_ROOT@@/bin/common/rpsbproc_data", # Do not change this. + }, + }, + + ] diff --git a/conf/modules.config b/conf/modules.config index 54124e45..7ed2b800 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -55,6 +55,9 @@ params { 'prokka' { args = "" } + 'dfast' { + args = "" + } 'multiqc' { args = "" } diff --git a/conf/test_dfast.config b/conf/test_dfast.config index 3b1f8a19..756554d9 100644 --- a/conf/test_dfast.config +++ b/conf/test_dfast.config @@ -27,4 +27,5 @@ params { annotation_tool = 'dfast' assembly_type = 'short' skip_pycoqc = true + skip_kraken2 = true } \ No newline at end of file diff --git a/modules/local/dfast.nf b/modules/local/dfast.nf new file mode 100644 index 00000000..0415ab13 --- /dev/null +++ b/modules/local/dfast.nf @@ -0,0 +1,36 @@ +// Import generic module functions +include { initOptions; saveFiles; getSoftwareName } from './functions' + +params.options = [:] +options = initOptions(params.options) + +process DFAST { + tag "$meta.id" + label 'process_medium' + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), publish_id:'') } + + conda (params.enable_conda ? "dfast=1.2.14" : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://depot.galaxyproject.org/singularity/dfast:1.2.14--h2e03b76_0" + } else { + container "quay.io/biocontainers/dfast:1.2.14--h2e03b76_0" + } + + input: + tuple val(meta), path(fasta) + file (config) + + output: + tuple val(meta), path("RESULT*"), emit: reads + path "*.version.txt" , emit: version + + script: + def software = getSoftwareName(task.process) + """ + dfast_file_downloader.py --protein dfast --dbroot . + dfast --genome ${fasta} --config $config + dfast --version | sed -e "s/DFAST ver. //g" > "${software}.version.txt" + """ +} diff --git a/nextflow.config b/nextflow.config index fb2d37fb..930aabb5 100644 --- a/nextflow.config +++ b/nextflow.config @@ -27,7 +27,7 @@ params { // Annotation annotation_tool = 'prokka' prokka_args = "" - dfast_config = "$baseDir/assets/test_config_dfast.py" + dfast_config = "$projectDir/assets/test_config_dfast.py" // Skipping options skip_kraken2 = false @@ -144,8 +144,12 @@ profiles { podman.enabled = false shifter.enabled = false } - test { includeConfig 'conf/test.config' } - test_full { includeConfig 'conf/test_full.config' } + test { includeConfig 'conf/test.config' } + test_dfast { includeConfig 'conf/test_dfast.config' } + test_hybrid { includeConfig 'conf/test_hybrid.config' } + test_long { includeConfig 'conf/test_long.config' } + test_long_miniasm { includeConfig 'conf/ttest_long_miniasmest.config' } + test_full { includeConfig 'conf/test_full.config' } } // Export these variables to prevent local Python/R libraries from conflicting with those in the container diff --git a/workflows/bacass.nf b/workflows/bacass.nf index cbf7d5a3..2f7ec183 100644 --- a/workflows/bacass.nf +++ b/workflows/bacass.nf @@ -55,6 +55,7 @@ include { SKEWER } from '../modules/local/skewer' include { NANOPLOT } from '../modules/local/nanoplot' addParams( options: modules['nanoplot'] ) include { PORECHOP } from '../modules/local/porechop' addParams( options: modules['porechop'] ) include { UNICYCLER } from '../modules/local/unicycler' addParams( options: unicycler_options ) +include { DFAST } from '../modules/local/dfast' addParams( options: modules['dfast'] ) // // SUBWORKFLOW: Consisting of a mix of local and nf-core/modules @@ -229,6 +230,18 @@ workflow BACASS { ch_software_versions = ch_software_versions.mix(PROKKA.out.version.first().ifEmpty(null)) } + // + // MODULE: DFAST, gene annotation + // + // TODO: "dfast_file_downloader.py --protein dfast --dbroot ." could be used in a separate process and the db could be forwarded + if ( !params.skip_annotation && params.annotation_tool == 'dfast' ) { + DFAST ( + UNICYCLER.out.scaffolds, + Channel.value(params.dfast_config ? file(params.dfast_config) : "") + ) + ch_software_versions = ch_software_versions.mix(DFAST.out.version.first().ifEmpty(null)) + } + // // MODULE: Pipeline reporting // From 090f175a879badb25e2989e4e28bd590e168b58a Mon Sep 17 00:00:00 2001 From: d4straub Date: Mon, 9 Aug 2021 12:40:33 +0200 Subject: [PATCH 29/81] modify test profiles --- conf/test_hybrid.config | 3 ++- conf/test_long.config | 4 +++- conf/test_long_miniasm.config | 3 ++- nextflow.config | 2 +- 4 files changed, 8 insertions(+), 4 deletions(-) diff --git a/conf/test_hybrid.config b/conf/test_hybrid.config index a436bb15..cd93e699 100644 --- a/conf/test_hybrid.config +++ b/conf/test_hybrid.config @@ -25,4 +25,5 @@ params { // some extra args to speed tests up assembly_type='hybrid' prokka_args=" --fast" -} \ No newline at end of file + skip_kraken2 = true +} diff --git a/conf/test_long.config b/conf/test_long.config index 07753f00..b5d344f0 100644 --- a/conf/test_long.config +++ b/conf/test_long.config @@ -27,4 +27,6 @@ params { canu_args = 'stopOnLowCoverage=0 minInputCoverage=0' assembly_type = 'long' skip_polish = true -} \ No newline at end of file + skip_kraken2 = true + assembler = 'canu' +} diff --git a/conf/test_long_miniasm.config b/conf/test_long_miniasm.config index 5b803016..c7719763 100644 --- a/conf/test_long_miniasm.config +++ b/conf/test_long_miniasm.config @@ -27,4 +27,5 @@ params { assembly_type = 'long' assembler = 'miniasm' skip_polish = true -} \ No newline at end of file + skip_kraken2 = true +} diff --git a/nextflow.config b/nextflow.config index 930aabb5..6e508967 100644 --- a/nextflow.config +++ b/nextflow.config @@ -148,7 +148,7 @@ profiles { test_dfast { includeConfig 'conf/test_dfast.config' } test_hybrid { includeConfig 'conf/test_hybrid.config' } test_long { includeConfig 'conf/test_long.config' } - test_long_miniasm { includeConfig 'conf/ttest_long_miniasmest.config' } + test_long_miniasm { includeConfig 'conf/test_long_miniasmest.config' } test_full { includeConfig 'conf/test_full.config' } } From bfa5b424a89ab95fdb533f0f00e494f4d590dffc Mon Sep 17 00:00:00 2001 From: d4straub Date: Mon, 9 Aug 2021 12:54:36 +0200 Subject: [PATCH 30/81] create assembly channel --- workflows/bacass.nf | 19 ++++++++++++------- 1 file changed, 12 insertions(+), 7 deletions(-) diff --git a/workflows/bacass.nf b/workflows/bacass.nf index 2f7ec183..dfb6ff13 100644 --- a/workflows/bacass.nf +++ b/workflows/bacass.nf @@ -156,22 +156,27 @@ workflow BACASS { SKEWER.out.reads .dump(tag: 'skewer') .join(PORECHOP.out.reads) - .dump(tag: 'unicycler') + .dump(tag: 'ch_for_assembly') .set { ch_for_assembly } } else if ( params.assembly_type == 'short' ) { SKEWER.out.reads .dump(tag: 'skewer') .map{ meta,reads -> tuple(meta,reads,'NA') } - .dump(tag: 'unicycler') + .dump(tag: 'ch_for_assembly') .set { ch_for_assembly } } else if ( params.assembly_type == 'long' ) { PORECHOP.out.reads .dump(tag: 'porechop') .map{ meta,lr -> tuple(meta,'NA',lr) } - .dump(tag: 'unicycler') + .dump(tag: 'ch_for_assembly') .set { ch_for_assembly } //old channel name: ch_short_long_joint_unicycler } + // + // ASSEMBLY: Unicycler, Canu, Miniasm (TODO: convert into subworkflow) + // + ch_assembly = Channel.empty() + // // MODULE: Unicycler, genome assembly, nf-core module allows only short assembly // @@ -179,6 +184,7 @@ workflow BACASS { UNICYCLER ( ch_for_assembly ) + ch_assembly = UNICYCLER.out.scaffolds.dump(tag: 'unicycler') ch_software_versions = ch_software_versions.mix(UNICYCLER.out.version.first().ifEmpty(null)) } @@ -204,8 +210,7 @@ workflow BACASS { // // MODULE: QUAST, assembly QC // - UNICYCLER.out.scaffolds - .dump(tag: 'unicycler') + ch_assembly .map { meta, fasta -> fasta } .collect() .set { ch_to_quast } @@ -223,7 +228,7 @@ workflow BACASS { // if ( !params.skip_annotation && params.annotation_tool == 'prokka' ) { PROKKA ( - UNICYCLER.out.scaffolds, + ch_assembly, [], [] ) @@ -236,7 +241,7 @@ workflow BACASS { // TODO: "dfast_file_downloader.py --protein dfast --dbroot ." could be used in a separate process and the db could be forwarded if ( !params.skip_annotation && params.annotation_tool == 'dfast' ) { DFAST ( - UNICYCLER.out.scaffolds, + ch_assembly, Channel.value(params.dfast_config ? file(params.dfast_config) : "") ) ch_software_versions = ch_software_versions.mix(DFAST.out.version.first().ifEmpty(null)) From 008f01e8e2080b8abd85cac8614431beac1bb583 Mon Sep 17 00:00:00 2001 From: d4straub Date: Mon, 9 Aug 2021 12:57:00 +0200 Subject: [PATCH 31/81] fix test_long_miniasm --- nextflow.config | 12 ++++++------ 1 file changed, 6 insertions(+), 6 deletions(-) diff --git a/nextflow.config b/nextflow.config index 6e508967..8dc8e31b 100644 --- a/nextflow.config +++ b/nextflow.config @@ -144,12 +144,12 @@ profiles { podman.enabled = false shifter.enabled = false } - test { includeConfig 'conf/test.config' } - test_dfast { includeConfig 'conf/test_dfast.config' } - test_hybrid { includeConfig 'conf/test_hybrid.config' } - test_long { includeConfig 'conf/test_long.config' } - test_long_miniasm { includeConfig 'conf/test_long_miniasmest.config' } - test_full { includeConfig 'conf/test_full.config' } + test { includeConfig 'conf/test.config' } + test_dfast { includeConfig 'conf/test_dfast.config' } + test_hybrid { includeConfig 'conf/test_hybrid.config' } + test_long { includeConfig 'conf/test_long.config' } + test_long_miniasm { includeConfig 'conf/test_long_miniasm.config' } + test_full { includeConfig 'conf/test_full.config' } } // Export these variables to prevent local Python/R libraries from conflicting with those in the container From 0d26ae8d92d5c94a19c4800cd67a73d8f2b8e41f Mon Sep 17 00:00:00 2001 From: d4straub Date: Mon, 9 Aug 2021 14:27:04 +0200 Subject: [PATCH 32/81] add canu but test fails because reads do not overlap --- conf/modules.config | 4 ++++ conf/test_long.config | 2 +- modules/local/canu.nf | 47 +++++++++++++++++++++++++++++++++++++++++++ workflows/bacass.nf | 15 ++++++++++++++ 4 files changed, 67 insertions(+), 1 deletion(-) create mode 100644 modules/local/canu.nf diff --git a/conf/modules.config b/conf/modules.config index 7ed2b800..6c3286fc 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -46,6 +46,10 @@ params { args = "" publish_dir = "./Unicycler" } + 'canu' { + args = "" + publish_dir = "./Canu" + } 'kraken2' { args = "" } diff --git a/conf/test_long.config b/conf/test_long.config index b5d344f0..2657c61e 100644 --- a/conf/test_long.config +++ b/conf/test_long.config @@ -24,7 +24,7 @@ params { // some extra args to speed tests up prokka_args = " --fast" - canu_args = 'stopOnLowCoverage=0 minInputCoverage=0' + canu_args = 'stopOnLowCoverage=0 minInputCoverage=0 corMinCoverage=0 minOverlapLength=0 minReadLength=0' assembly_type = 'long' skip_polish = true skip_kraken2 = true diff --git a/modules/local/canu.nf b/modules/local/canu.nf new file mode 100644 index 00000000..016da8bf --- /dev/null +++ b/modules/local/canu.nf @@ -0,0 +1,47 @@ +// Import generic module functions +include { initOptions; saveFiles; getSoftwareName } from './functions' + +params.options = [:] +options = initOptions(params.options) + +process CANU { + tag "$meta.id" + label 'process_high' + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } + + conda (params.enable_conda ? 'canu=2.1.1-2' : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://depot.galaxyproject.org/singularity/canu:2.1.1--h1b792b2_2" + } else { + container "quay.io/biocontainers/canu:2.1.1--h1b792b2_2" + } + + input: + tuple val(meta), val(reads), file(longreads) + + output: + tuple val(meta), path('*_assembly.fasta') , emit: assembly + tuple val(meta), path('*_assembly.report'), emit: log + path '*.version.txt' , emit: version + + script: + def software = getSoftwareName(task.process) + def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" + def genomeSize = meta.genome_size == 'NA' ? "5m" : "${meta.genome_size}" + """ + canu -p assembly -d canu_out \ + ${options.args} \ + genomeSize="${genomeSize}" -nanopore "${longreads}" \ + maxThreads="${task.cpus}" merylMemory="${task.memory.toGiga()}G" \ + merylThreads="${task.cpus}" hapThreads="${task.cpus}" batMemory="${task.memory.toGiga()}G" \ + redMemory="${task.memory.toGiga()}G" redThreads="${task.cpus}" \ + oeaMemory="${task.memory.toGiga()}G" oeaThreads="${task.cpus}" \ + corMemory="${task.memory.toGiga()}G" corThreads="${task.cpus}" + mv canu_out/assembly.contigs.fasta ${prefix}_assembly.fasta + mv canu_out/assembly.report ${prefix}_assembly.report + + echo \$(canu --version 2>&1) | sed -e 's/Canu //g' > ${software}.version.txt + """ +} diff --git a/workflows/bacass.nf b/workflows/bacass.nf index dfb6ff13..72fa53da 100644 --- a/workflows/bacass.nf +++ b/workflows/bacass.nf @@ -47,6 +47,9 @@ def modules = params.modules.clone() def unicycler_options = modules['unicycler'] unicycler_options.args += " $params.unicycler_args" +def canu_options = modules['canu'] +canu_options.args += " $params.canu_args" + // // MODULE: Local to the pipeline // @@ -55,6 +58,7 @@ include { SKEWER } from '../modules/local/skewer' include { NANOPLOT } from '../modules/local/nanoplot' addParams( options: modules['nanoplot'] ) include { PORECHOP } from '../modules/local/porechop' addParams( options: modules['porechop'] ) include { UNICYCLER } from '../modules/local/unicycler' addParams( options: unicycler_options ) +include { CANU } from '../modules/local/canu' addParams( options: canu_options ) include { DFAST } from '../modules/local/dfast' addParams( options: modules['dfast'] ) // @@ -188,6 +192,17 @@ workflow BACASS { ch_software_versions = ch_software_versions.mix(UNICYCLER.out.version.first().ifEmpty(null)) } + // + // MODULE: Unicycler, genome assembly, nf-core module allows only short assembly + // + if ( params.assembler == 'canu' ) { + CANU ( + ch_for_assembly + ) + //ch_assembly = CANU.out.assembly.dump(tag: 'canu') //needs to go into nanopolish & medaka first! + ch_software_versions = ch_software_versions.mix(CANU.out.version.first().ifEmpty(null)) + } + // // MODULE: Kraken2, QC for sample purity // From e92471ff7d8d498210670ef50e2fa16e8884a8eb Mon Sep 17 00:00:00 2001 From: d4straub Date: Mon, 9 Aug 2021 15:19:24 +0200 Subject: [PATCH 33/81] add miniasm assembly --- conf/modules.config | 8 +++++++ modules/local/miniasm.nf | 38 +++++++++++++++++++++++++++++++ modules/local/minimap_align.nf | 41 ++++++++++++++++++++++++++++++++++ workflows/bacass.nf | 19 +++++++++++++++- 4 files changed, 105 insertions(+), 1 deletion(-) create mode 100644 modules/local/miniasm.nf create mode 100644 modules/local/minimap_align.nf diff --git a/conf/modules.config b/conf/modules.config index 6c3286fc..5edcf47d 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -50,6 +50,14 @@ params { args = "" publish_dir = "./Canu" } + 'minimap2_align' { + args = "-x ava-ont" + publish_files = false + } + 'miniasm' { + args = "" + publish_dir = "./Miniasm" + } 'kraken2' { args = "" } diff --git a/modules/local/miniasm.nf b/modules/local/miniasm.nf new file mode 100644 index 00000000..10ae6441 --- /dev/null +++ b/modules/local/miniasm.nf @@ -0,0 +1,38 @@ +// Import generic module functions +include { initOptions; saveFiles; getSoftwareName } from './functions' + +params.options = [:] +options = initOptions(params.options) + +process MINIASM { + tag "$meta.id" + label 'process_high' + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } + + conda (params.enable_conda ? 'bioconda::miniasm=0.3_r179' : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://depot.galaxyproject.org/singularity/miniasm:0.3_r179--h5bf99c6_2" + } else { + container "quay.io/biocontainers/miniasm:0.3_r179--h5bf99c6_2" + } + + input: + tuple val(meta), val(reads), file(longreads), path(paf) + + output: + tuple val(meta), path('*_assembly.fasta') , emit: assembly + //tuple val(meta), path('*_assembly.report'), emit: log + path '*.version.txt' , emit: version + + script: + def software = getSoftwareName(task.process) + def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" + """ + miniasm -f "${longreads}" "${paf}" > "${longreads}.gfa" + awk '/^S/{print ">"\$2"\\n"\$3}' "${longreads}.gfa" | fold > ${prefix}_assembly.fasta + + echo \$(miniasm -V 2>&1) > ${software}.version.txt + """ +} diff --git a/modules/local/minimap_align.nf b/modules/local/minimap_align.nf new file mode 100644 index 00000000..5ec50fd9 --- /dev/null +++ b/modules/local/minimap_align.nf @@ -0,0 +1,41 @@ +// Import generic module functions +include { initOptions; saveFiles; getSoftwareName } from './functions' + +params.options = [:] +options = initOptions(params.options) + +process MINIMAP2_ALIGN { + tag "$meta.id" + label 'process_medium' + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } + + conda (params.enable_conda ? 'bioconda::minimap2=2.21' : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://depot.galaxyproject.org/singularity/minimap2:2.21--h5bf99c6_0" + } else { + container "quay.io/biocontainers/minimap2:2.21--h5bf99c6_0" + } + + input: + tuple val(meta), val(reads), file(longreads), file('reference') + + output: + tuple val(meta), val(reads), file(longreads), path("*.paf"), emit: paf + path "*.version.txt", emit: version + + script: + def software = getSoftwareName(task.process) + def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" + """ + minimap2 \\ + $options.args \\ + -t $task.cpus \\ + reference \\ + $longreads \\ + > ${prefix}.paf + + echo \$(minimap2 --version 2>&1) > ${software}.version.txt + """ +} diff --git a/workflows/bacass.nf b/workflows/bacass.nf index 72fa53da..3eeaa30b 100644 --- a/workflows/bacass.nf +++ b/workflows/bacass.nf @@ -59,6 +59,8 @@ include { NANOPLOT } from '../modules/local/nanoplot' include { PORECHOP } from '../modules/local/porechop' addParams( options: modules['porechop'] ) include { UNICYCLER } from '../modules/local/unicycler' addParams( options: unicycler_options ) include { CANU } from '../modules/local/canu' addParams( options: canu_options ) +include { MINIMAP2_ALIGN } from '../modules/local/minimap_align' addParams( options: modules['minimap2_align'] ) +include { MINIASM } from '../modules/local/miniasm' addParams( options: modules['miniasm'] ) include { DFAST } from '../modules/local/dfast' addParams( options: modules['dfast'] ) // @@ -193,7 +195,7 @@ workflow BACASS { } // - // MODULE: Unicycler, genome assembly, nf-core module allows only short assembly + // MODULE: Canu, genome assembly, long reads // if ( params.assembler == 'canu' ) { CANU ( @@ -203,6 +205,21 @@ workflow BACASS { ch_software_versions = ch_software_versions.mix(CANU.out.version.first().ifEmpty(null)) } + // + // MODULE: Miniasm, genome assembly, long reads + // + if ( params.assembler == 'miniasm' ) { + MINIMAP2_ALIGN ( + ch_for_assembly.map{ meta,sr,lr -> tuple(meta,sr,lr,lr) } + ) + ch_software_versions = ch_software_versions.mix(MINIMAP2_ALIGN.out.version.first().ifEmpty(null)) + MINIASM ( + MINIMAP2_ALIGN.out.paf.dump(tag: 'minimap2') + ) + //ch_assembly = MINIASM.out.assembly.dump(tag: 'miniasm') //needs to go into consensus -> nanopolish & medaka first! + ch_software_versions = ch_software_versions.mix(MINIASM.out.version.first().ifEmpty(null)) + } + // // MODULE: Kraken2, QC for sample purity // From b6fc4d7e92f664d79b64304d777a4d2d17f2bfdc Mon Sep 17 00:00:00 2001 From: d4straub Date: Mon, 9 Aug 2021 15:31:13 +0200 Subject: [PATCH 34/81] canu test_long: bacass_long_miniasm.csv instead bacass_long.csv --- conf/test_long.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/conf/test_long.config b/conf/test_long.config index 2657c61e..f1fb04da 100644 --- a/conf/test_long.config +++ b/conf/test_long.config @@ -20,7 +20,7 @@ params { max_time = 6.h // Input data - input = 'https://raw.githubusercontent.com/nf-core/test-datasets/bacass/bacass_long.csv' + input = 'https://raw.githubusercontent.com/nf-core/test-datasets/bacass/bacass_long_miniasm.csv' // some extra args to speed tests up prokka_args = " --fast" From 94a0f7d5205d599b01205504b56389e8c4e4acc9 Mon Sep 17 00:00:00 2001 From: d4straub Date: Mon, 9 Aug 2021 16:17:28 +0200 Subject: [PATCH 35/81] add consensus (racon) for miniasm --- conf/modules.config | 10 ++++++++-- modules/local/miniasm.nf | 4 ++-- modules/local/minimap_align.nf | 2 +- modules/local/racon.nf | 36 ++++++++++++++++++++++++++++++++++ workflows/bacass.nf | 29 ++++++++++++++++++++++++++- 5 files changed, 75 insertions(+), 6 deletions(-) create mode 100644 modules/local/racon.nf diff --git a/conf/modules.config b/conf/modules.config index 5edcf47d..fa34376f 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -50,14 +50,20 @@ params { args = "" publish_dir = "./Canu" } - 'minimap2_align' { - args = "-x ava-ont" + 'minimap_align' { + args = "" publish_files = false } 'miniasm' { args = "" + publish_files = [ '_assembly.fasta':'' ] publish_dir = "./Miniasm" } + 'racon' { + args = "" + publish_files = [ '_assembly_consensus.fasta':'' ] + publish_dir = "./Miniasm/Racon" + } 'kraken2' { args = "" } diff --git a/modules/local/miniasm.nf b/modules/local/miniasm.nf index 10ae6441..acfd298c 100644 --- a/modules/local/miniasm.nf +++ b/modules/local/miniasm.nf @@ -19,11 +19,11 @@ process MINIASM { } input: - tuple val(meta), val(reads), file(longreads), path(paf) + tuple val(meta), val(reads), file(longreads), file(assembly), path(paf) output: tuple val(meta), path('*_assembly.fasta') , emit: assembly - //tuple val(meta), path('*_assembly.report'), emit: log + tuple val(meta), val(reads), file(longreads), path('*_assembly.fasta') , emit: all path '*.version.txt' , emit: version script: diff --git a/modules/local/minimap_align.nf b/modules/local/minimap_align.nf index 5ec50fd9..89af8661 100644 --- a/modules/local/minimap_align.nf +++ b/modules/local/minimap_align.nf @@ -22,7 +22,7 @@ process MINIMAP2_ALIGN { tuple val(meta), val(reads), file(longreads), file('reference') output: - tuple val(meta), val(reads), file(longreads), path("*.paf"), emit: paf + tuple val(meta), val(reads), file(longreads), file('reference'), path("*.paf"), emit: paf path "*.version.txt", emit: version script: diff --git a/modules/local/racon.nf b/modules/local/racon.nf new file mode 100644 index 00000000..b7331706 --- /dev/null +++ b/modules/local/racon.nf @@ -0,0 +1,36 @@ +// Import generic module functions +include { initOptions; saveFiles; getSoftwareName } from './functions' + +params.options = [:] +options = initOptions(params.options) + +process RACON { + tag "$meta.id" + label 'process_high' + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } + + conda (params.enable_conda ? 'racon=1.4.20-1' : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://depot.galaxyproject.org/singularity/racon:1.4.20--h9a82719_1" + } else { + container "quay.io/biocontainers/racon:1.4.20--h9a82719_1" + } + + input: + tuple val(meta), val(reads), file(longreads), path('assembly.fasta'), path(paf) + + output: + tuple val(meta), path('*_assembly_consensus.fasta') , emit: assembly + path '*.version.txt' , emit: version + + script: + def software = getSoftwareName(task.process) + def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" + """ + racon -t "${task.cpus}" "${longreads}" "${paf}" "assembly.fasta" > ${prefix}_assembly_consensus.fasta + + echo \$(racon -v 2>&1) > ${software}.version.txt + """ +} diff --git a/workflows/bacass.nf b/workflows/bacass.nf index 3eeaa30b..05e4966e 100644 --- a/workflows/bacass.nf +++ b/workflows/bacass.nf @@ -50,6 +50,12 @@ unicycler_options.args += " $params.unicycler_args" def canu_options = modules['canu'] canu_options.args += " $params.canu_args" +def minimap_align_options = modules['minimap_align'] +minimap_align_options.args += " -x ava-ont" + +def minimap_consensus_options = modules['minimap_align'] +minimap_consensus_options.args += " -x map-ont" + // // MODULE: Local to the pipeline // @@ -59,8 +65,10 @@ include { NANOPLOT } from '../modules/local/nanoplot' include { PORECHOP } from '../modules/local/porechop' addParams( options: modules['porechop'] ) include { UNICYCLER } from '../modules/local/unicycler' addParams( options: unicycler_options ) include { CANU } from '../modules/local/canu' addParams( options: canu_options ) -include { MINIMAP2_ALIGN } from '../modules/local/minimap_align' addParams( options: modules['minimap2_align'] ) +include { MINIMAP2_ALIGN } from '../modules/local/minimap_align' addParams( options: minimap_align_options ) +include { MINIMAP2_ALIGN as MINIMAP2_CONSENSUS } from '../modules/local/minimap_align' addParams( options: minimap_consensus_options) include { MINIASM } from '../modules/local/miniasm' addParams( options: modules['miniasm'] ) +include { RACON } from '../modules/local/racon' addParams( options: modules['racon'] ) include { DFAST } from '../modules/local/dfast' addParams( options: modules['dfast'] ) // @@ -218,8 +226,27 @@ workflow BACASS { ) //ch_assembly = MINIASM.out.assembly.dump(tag: 'miniasm') //needs to go into consensus -> nanopolish & medaka first! ch_software_versions = ch_software_versions.mix(MINIASM.out.version.first().ifEmpty(null)) + MINIMAP2_CONSENSUS ( + MINIASM.out.all.dump(tag: 'miniasm') + ) + RACON ( + MINIMAP2_CONSENSUS.out.paf.dump(tag: 'minimap2_consensus') + ) + ch_software_versions = ch_software_versions.mix(RACON.out.version.first().ifEmpty(null)) } + // + // MODULE: Nanopolish, polishes assembly using FAST5 files - should take either miniasm, canu, or unicycler consensus sequence + // + //TODO + //when: !params.skip_polish && params.assembly_type == 'long' && params.polish_method != 'medaka' + + // + // MODULE: Medaka, polishes assembly - should take either miniasm, canu, or unicycler consensus sequence + // + //TODO + //when: !params.skip_polish && params.assembly_type == 'long' && params.polish_method == 'medaka' + // // MODULE: Kraken2, QC for sample purity // From 6644546d95bec55d666a1a0213cb95b3a5b2ae4e Mon Sep 17 00:00:00 2001 From: d4straub Date: Mon, 9 Aug 2021 16:22:34 +0200 Subject: [PATCH 36/81] fix racon version output --- modules/local/racon.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/modules/local/racon.nf b/modules/local/racon.nf index b7331706..f1f1cad5 100644 --- a/modules/local/racon.nf +++ b/modules/local/racon.nf @@ -31,6 +31,6 @@ process RACON { """ racon -t "${task.cpus}" "${longreads}" "${paf}" "assembly.fasta" > ${prefix}_assembly_consensus.fasta - echo \$(racon -v 2>&1) > ${software}.version.txt + echo \$(racon --version 2>&1) | sed 's/^.*v//' > ${software}.version.txt """ } From 931c9c4c433fcdbfd0f357dfa60a8a5a0c042e01 Mon Sep 17 00:00:00 2001 From: d4straub Date: Fri, 13 Aug 2021 16:44:01 +0200 Subject: [PATCH 37/81] fix test_long --- conf/test_long.config | 1 - 1 file changed, 1 deletion(-) diff --git a/conf/test_long.config b/conf/test_long.config index f1fb04da..d87d3765 100644 --- a/conf/test_long.config +++ b/conf/test_long.config @@ -28,5 +28,4 @@ params { assembly_type = 'long' skip_polish = true skip_kraken2 = true - assembler = 'canu' } From a6e62a66889b9030ff8cc67fb25083216ece3a96 Mon Sep 17 00:00:00 2001 From: d4straub Date: Fri, 13 Aug 2021 17:18:03 +0200 Subject: [PATCH 38/81] add medaka --- conf/modules.config | 4 ++++ modules/local/medaka.nf | 40 ++++++++++++++++++++++++++++++++++++++++ workflows/bacass.nf | 16 +++++++++++----- 3 files changed, 55 insertions(+), 5 deletions(-) create mode 100644 modules/local/medaka.nf diff --git a/conf/modules.config b/conf/modules.config index fa34376f..a9710f79 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -64,6 +64,10 @@ params { publish_files = [ '_assembly_consensus.fasta':'' ] publish_dir = "./Miniasm/Racon" } + 'medaka' { + args = "" + publish_dir = "./Medaka" + } 'kraken2' { args = "" } diff --git a/modules/local/medaka.nf b/modules/local/medaka.nf new file mode 100644 index 00000000..6e8574e5 --- /dev/null +++ b/modules/local/medaka.nf @@ -0,0 +1,40 @@ +// Import generic module functions +include { initOptions; saveFiles; getSoftwareName } from './functions' + +params.options = [:] +options = initOptions(params.options) + +process MEDAKA { + tag "$meta.id" + label 'process_high' + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } + + conda (params.enable_conda ? 'medaka=1.4.3-0' : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://depot.galaxyproject.org/singularity/medaka:1.4.3--py38h130def0_0" + } else { + container "quay.io/biocontainers/medaka:1.4.3--py38h130def0_0" + } + + input: + tuple val(meta), file(assembly), val(reads), file(longreads) + + output: + tuple val(meta), path('*_polished_genome.fa'), emit: assembly + path '*.version.txt' , emit: version + + script: + def software = getSoftwareName(task.process) + def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" + """ + medaka_consensus ${options.args} \ + -i ${longreads} \ + -d ${assembly} \ + -o "${prefix}_polished_genome.fa" \ + -t ${task.cpus} + + echo \$(medaka --version 2>&1) | sed -e 's/medaka //g' > ${software}.version.txt + """ +} diff --git a/workflows/bacass.nf b/workflows/bacass.nf index 05e4966e..91860e42 100644 --- a/workflows/bacass.nf +++ b/workflows/bacass.nf @@ -69,6 +69,7 @@ include { MINIMAP2_ALIGN } from '../modules/local/minimap_align' include { MINIMAP2_ALIGN as MINIMAP2_CONSENSUS } from '../modules/local/minimap_align' addParams( options: minimap_consensus_options) include { MINIASM } from '../modules/local/miniasm' addParams( options: modules['miniasm'] ) include { RACON } from '../modules/local/racon' addParams( options: modules['racon'] ) +include { MEDAKA } from '../modules/local/medaka' addParams( options: modules['medaka'] ) include { DFAST } from '../modules/local/dfast' addParams( options: modules['dfast'] ) // @@ -198,7 +199,7 @@ workflow BACASS { UNICYCLER ( ch_for_assembly ) - ch_assembly = UNICYCLER.out.scaffolds.dump(tag: 'unicycler') + ch_assembly = ch_assembly.mix( UNICYCLER.out.scaffolds.dump(tag: 'unicycler') ) ch_software_versions = ch_software_versions.mix(UNICYCLER.out.version.first().ifEmpty(null)) } @@ -209,7 +210,7 @@ workflow BACASS { CANU ( ch_for_assembly ) - //ch_assembly = CANU.out.assembly.dump(tag: 'canu') //needs to go into nanopolish & medaka first! + ch_assembly = ch_assembly.mix( CANU.out.assembly.dump(tag: 'canu') ) ch_software_versions = ch_software_versions.mix(CANU.out.version.first().ifEmpty(null)) } @@ -224,7 +225,6 @@ workflow BACASS { MINIASM ( MINIMAP2_ALIGN.out.paf.dump(tag: 'minimap2') ) - //ch_assembly = MINIASM.out.assembly.dump(tag: 'miniasm') //needs to go into consensus -> nanopolish & medaka first! ch_software_versions = ch_software_versions.mix(MINIASM.out.version.first().ifEmpty(null)) MINIMAP2_CONSENSUS ( MINIASM.out.all.dump(tag: 'miniasm') @@ -232,6 +232,7 @@ workflow BACASS { RACON ( MINIMAP2_CONSENSUS.out.paf.dump(tag: 'minimap2_consensus') ) + ch_assembly = ch_assembly.mix( MINIASM.out.assembly.dump(tag: 'miniasm') ) ch_software_versions = ch_software_versions.mix(RACON.out.version.first().ifEmpty(null)) } @@ -244,8 +245,13 @@ workflow BACASS { // // MODULE: Medaka, polishes assembly - should take either miniasm, canu, or unicycler consensus sequence // - //TODO - //when: !params.skip_polish && params.assembly_type == 'long' && params.polish_method == 'medaka' + if ( !params.skip_polish && params.assembly_type == 'long' && params.polish_method == 'medaka' ) { + ch_assembly + .join( ch_for_assembly ) + .set { ch_for_medaka } + MEDAKA ( ch_for_medaka.dump(tag: 'into_medaka') ) + ch_software_versions = ch_software_versions.mix(MEDAKA.out.version.first().ifEmpty(null)) + } // // MODULE: Kraken2, QC for sample purity From d00f08bb5ae9902af28b31b9dadc25569b5b7e72 Mon Sep 17 00:00:00 2001 From: d4straub Date: Mon, 16 Aug 2021 14:42:25 +0200 Subject: [PATCH 39/81] add nanopolish --- conf/modules.config | 4 ++ modules.json | 6 ++ modules/local/nanopolish.nf | 52 ++++++++++++++ .../modules/samtools/index/functions.nf | 68 +++++++++++++++++++ .../nf-core/modules/samtools/index/main.nf | 35 ++++++++++ .../nf-core/modules/samtools/index/meta.yml | 47 +++++++++++++ .../modules/samtools/sort/functions.nf | 68 +++++++++++++++++++ modules/nf-core/modules/samtools/sort/main.nf | 35 ++++++++++ .../nf-core/modules/samtools/sort/meta.yml | 43 ++++++++++++ workflows/bacass.nf | 36 +++++++++- 10 files changed, 391 insertions(+), 3 deletions(-) create mode 100644 modules/local/nanopolish.nf create mode 100644 modules/nf-core/modules/samtools/index/functions.nf create mode 100644 modules/nf-core/modules/samtools/index/main.nf create mode 100644 modules/nf-core/modules/samtools/index/meta.yml create mode 100644 modules/nf-core/modules/samtools/sort/functions.nf create mode 100644 modules/nf-core/modules/samtools/sort/main.nf create mode 100644 modules/nf-core/modules/samtools/sort/meta.yml diff --git a/conf/modules.config b/conf/modules.config index a9710f79..919cebf4 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -68,6 +68,10 @@ params { args = "" publish_dir = "./Medaka" } + 'nanopolish' { + args = "" + publish_dir = "./Nanopolish" + } 'kraken2' { args = "" } diff --git a/modules.json b/modules.json index f81715a1..1103fbc5 100644 --- a/modules.json +++ b/modules.json @@ -20,6 +20,12 @@ }, "quast": { "git_sha": "e937c7950af70930d1f34bb961403d9d2aa81c7d" + }, + "samtools/index": { + "git_sha": "c5235a983d454787fa0c3247b02086969217163b" + }, + "samtools/sort": { + "git_sha": "c5235a983d454787fa0c3247b02086969217163b" } } } diff --git a/modules/local/nanopolish.nf b/modules/local/nanopolish.nf new file mode 100644 index 00000000..d74bf451 --- /dev/null +++ b/modules/local/nanopolish.nf @@ -0,0 +1,52 @@ +// Import generic module functions +include { initOptions; saveFiles; getSoftwareName } from './functions' + +params.options = [:] +options = initOptions(params.options) + +process NANOPOLISH { + tag "$meta.id" + label 'process_high' + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } + + conda (params.enable_conda ? 'nanopolish=0.13.2-5' : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://depot.galaxyproject.org/singularity/nanopolish:0.13.2--h8cec615_5" + } else { + container "quay.io/biocontainers/nanopolish:0.13.2--h8cec615_5" + } + + input: + tuple val(meta), val(reads), file(longreads), file(assembly), file(bai), file(fast5) + + output: + tuple val(meta), file('polished_genome.fa'), emit: assembly + path "*.version.txt", emit: version + + script: + def software = getSoftwareName(task.process) + def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" + """ + nanopolish index -d "${fast5}" "${longreads}" + + nanopolish_makerange.py "${assembly}" | \ + parallel \ + --results nanopolish.results \ + -P "${task.cpus}" \ + nanopolish variants \ + --consensus \ + -o polished.{1}.vcf \ + -w {1} \ + -r "${longreads}" \ + -b "${prefix}.bam" \ + -g "${assembly}" \ + -t "${task.cpus}" \ + --min-candidate-frequency 0.1 + + nanopolish vcf2fasta -g "${assembly}" polished.*.vcf > polished_genome.fa + + nanopolish --version | sed -e "s/nanopolish version //g" | head -n 1 > ${software}.version.txt + """ +} diff --git a/modules/nf-core/modules/samtools/index/functions.nf b/modules/nf-core/modules/samtools/index/functions.nf new file mode 100644 index 00000000..da9da093 --- /dev/null +++ b/modules/nf-core/modules/samtools/index/functions.nf @@ -0,0 +1,68 @@ +// +// Utility functions used in nf-core DSL2 module files +// + +// +// Extract name of software tool from process name using $task.process +// +def getSoftwareName(task_process) { + return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() +} + +// +// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules +// +def initOptions(Map args) { + def Map options = [:] + options.args = args.args ?: '' + options.args2 = args.args2 ?: '' + options.args3 = args.args3 ?: '' + options.publish_by_meta = args.publish_by_meta ?: [] + options.publish_dir = args.publish_dir ?: '' + options.publish_files = args.publish_files + options.suffix = args.suffix ?: '' + return options +} + +// +// Tidy up and join elements of a list to return a path string +// +def getPathFromList(path_list) { + def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries + paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes + return paths.join('/') +} + +// +// Function to save/publish module results +// +def saveFiles(Map args) { + if (!args.filename.endsWith('.version.txt')) { + def ioptions = initOptions(args.options) + def path_list = [ ioptions.publish_dir ?: args.publish_dir ] + if (ioptions.publish_by_meta) { + def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta + for (key in key_list) { + if (args.meta && key instanceof String) { + def path = key + if (args.meta.containsKey(key)) { + path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] + } + path = path instanceof String ? path : '' + path_list.add(path) + } + } + } + if (ioptions.publish_files instanceof Map) { + for (ext in ioptions.publish_files) { + if (args.filename.endsWith(ext.key)) { + def ext_list = path_list.collect() + ext_list.add(ext.value) + return "${getPathFromList(ext_list)}/$args.filename" + } + } + } else if (ioptions.publish_files == null) { + return "${getPathFromList(path_list)}/$args.filename" + } + } +} diff --git a/modules/nf-core/modules/samtools/index/main.nf b/modules/nf-core/modules/samtools/index/main.nf new file mode 100644 index 00000000..e1966fb3 --- /dev/null +++ b/modules/nf-core/modules/samtools/index/main.nf @@ -0,0 +1,35 @@ +// Import generic module functions +include { initOptions; saveFiles; getSoftwareName } from './functions' + +params.options = [:] +options = initOptions(params.options) + +process SAMTOOLS_INDEX { + tag "$meta.id" + label 'process_low' + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } + + conda (params.enable_conda ? 'bioconda::samtools=1.13' : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://depot.galaxyproject.org/singularity/samtools:1.13--h8c37831_0" + } else { + container "quay.io/biocontainers/samtools:1.13--h8c37831_0" + } + + input: + tuple val(meta), path(bam) + + output: + tuple val(meta), path("*.bai"), optional:true, emit: bai + tuple val(meta), path("*.csi"), optional:true, emit: csi + path "*.version.txt" , emit: version + + script: + def software = getSoftwareName(task.process) + """ + samtools index $options.args $bam + echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//' > ${software}.version.txt + """ +} diff --git a/modules/nf-core/modules/samtools/index/meta.yml b/modules/nf-core/modules/samtools/index/meta.yml new file mode 100644 index 00000000..5d076e3b --- /dev/null +++ b/modules/nf-core/modules/samtools/index/meta.yml @@ -0,0 +1,47 @@ +name: samtools_index +description: Index SAM/BAM/CRAM file +keywords: + - index + - bam + - sam + - cram +tools: + - samtools: + description: | + SAMtools is a set of utilities for interacting with and post-processing + short DNA sequence read alignments in the SAM, BAM and CRAM formats, written by Heng Li. + These files are generated as output by short read aligners like BWA. + homepage: http://www.htslib.org/ + documentation: hhttp://www.htslib.org/doc/samtools.html + doi: 10.1093/bioinformatics/btp352 +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - bam: + type: file + description: BAM/CRAM/SAM file + pattern: "*.{bam,cram,sam}" +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - bai: + type: file + description: BAM/CRAM/SAM index file + pattern: "*.{bai,crai,sai}" + - csi: + type: file + description: CSI index file + pattern: "*.{csi}" + - version: + type: file + description: File containing software version + pattern: "*.{version.txt}" +authors: + - "@drpatelh" + - "@ewels" diff --git a/modules/nf-core/modules/samtools/sort/functions.nf b/modules/nf-core/modules/samtools/sort/functions.nf new file mode 100644 index 00000000..da9da093 --- /dev/null +++ b/modules/nf-core/modules/samtools/sort/functions.nf @@ -0,0 +1,68 @@ +// +// Utility functions used in nf-core DSL2 module files +// + +// +// Extract name of software tool from process name using $task.process +// +def getSoftwareName(task_process) { + return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() +} + +// +// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules +// +def initOptions(Map args) { + def Map options = [:] + options.args = args.args ?: '' + options.args2 = args.args2 ?: '' + options.args3 = args.args3 ?: '' + options.publish_by_meta = args.publish_by_meta ?: [] + options.publish_dir = args.publish_dir ?: '' + options.publish_files = args.publish_files + options.suffix = args.suffix ?: '' + return options +} + +// +// Tidy up and join elements of a list to return a path string +// +def getPathFromList(path_list) { + def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries + paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes + return paths.join('/') +} + +// +// Function to save/publish module results +// +def saveFiles(Map args) { + if (!args.filename.endsWith('.version.txt')) { + def ioptions = initOptions(args.options) + def path_list = [ ioptions.publish_dir ?: args.publish_dir ] + if (ioptions.publish_by_meta) { + def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta + for (key in key_list) { + if (args.meta && key instanceof String) { + def path = key + if (args.meta.containsKey(key)) { + path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] + } + path = path instanceof String ? path : '' + path_list.add(path) + } + } + } + if (ioptions.publish_files instanceof Map) { + for (ext in ioptions.publish_files) { + if (args.filename.endsWith(ext.key)) { + def ext_list = path_list.collect() + ext_list.add(ext.value) + return "${getPathFromList(ext_list)}/$args.filename" + } + } + } else if (ioptions.publish_files == null) { + return "${getPathFromList(path_list)}/$args.filename" + } + } +} diff --git a/modules/nf-core/modules/samtools/sort/main.nf b/modules/nf-core/modules/samtools/sort/main.nf new file mode 100644 index 00000000..0a6b7048 --- /dev/null +++ b/modules/nf-core/modules/samtools/sort/main.nf @@ -0,0 +1,35 @@ +// Import generic module functions +include { initOptions; saveFiles; getSoftwareName } from './functions' + +params.options = [:] +options = initOptions(params.options) + +process SAMTOOLS_SORT { + tag "$meta.id" + label 'process_medium' + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } + + conda (params.enable_conda ? 'bioconda::samtools=1.13' : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://depot.galaxyproject.org/singularity/samtools:1.13--h8c37831_0" + } else { + container "quay.io/biocontainers/samtools:1.13--h8c37831_0" + } + + input: + tuple val(meta), path(bam) + + output: + tuple val(meta), path("*.bam"), emit: bam + path "*.version.txt" , emit: version + + script: + def software = getSoftwareName(task.process) + def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" + """ + samtools sort $options.args -@ $task.cpus -o ${prefix}.bam -T $prefix $bam + echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//' > ${software}.version.txt + """ +} diff --git a/modules/nf-core/modules/samtools/sort/meta.yml b/modules/nf-core/modules/samtools/sort/meta.yml new file mode 100644 index 00000000..704e8c1f --- /dev/null +++ b/modules/nf-core/modules/samtools/sort/meta.yml @@ -0,0 +1,43 @@ +name: samtools_sort +description: Sort SAM/BAM/CRAM file +keywords: + - sort + - bam + - sam + - cram +tools: + - samtools: + description: | + SAMtools is a set of utilities for interacting with and post-processing + short DNA sequence read alignments in the SAM, BAM and CRAM formats, written by Heng Li. + These files are generated as output by short read aligners like BWA. + homepage: http://www.htslib.org/ + documentation: hhttp://www.htslib.org/doc/samtools.html + doi: 10.1093/bioinformatics/btp352 +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - bam: + type: file + description: BAM/CRAM/SAM file + pattern: "*.{bam,cram,sam}" +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - bam: + type: file + description: Sorted BAM/CRAM/SAM file + pattern: "*.{bam,cram,sam}" + - version: + type: file + description: File containing software version + pattern: "*.{version.txt}" +authors: + - "@drpatelh" + - "@ewels" diff --git a/workflows/bacass.nf b/workflows/bacass.nf index 91860e42..00dcbe37 100644 --- a/workflows/bacass.nf +++ b/workflows/bacass.nf @@ -56,6 +56,9 @@ minimap_align_options.args += " -x ava-ont" def minimap_consensus_options = modules['minimap_align'] minimap_consensus_options.args += " -x map-ont" +def minimap_polish_options = modules['minimap_align'] +minimap_polish_options.args += " -ax map-ont" + // // MODULE: Local to the pipeline // @@ -67,9 +70,11 @@ include { UNICYCLER } from '../modules/local/unicycler' include { CANU } from '../modules/local/canu' addParams( options: canu_options ) include { MINIMAP2_ALIGN } from '../modules/local/minimap_align' addParams( options: minimap_align_options ) include { MINIMAP2_ALIGN as MINIMAP2_CONSENSUS } from '../modules/local/minimap_align' addParams( options: minimap_consensus_options) +include { MINIMAP2_ALIGN as MINIMAP2_POLISH } from '../modules/local/minimap_align' addParams( options: minimap_polish_options ) include { MINIASM } from '../modules/local/miniasm' addParams( options: modules['miniasm'] ) include { RACON } from '../modules/local/racon' addParams( options: modules['racon'] ) include { MEDAKA } from '../modules/local/medaka' addParams( options: modules['medaka'] ) +include { NANOPOLISH } from '../modules/local/nanopolish' addParams( options: modules['nanopolish'] ) include { DFAST } from '../modules/local/dfast' addParams( options: modules['dfast'] ) // @@ -94,7 +99,9 @@ prokka_options.args += " $params.prokka_args" // include { FASTQC } from '../modules/nf-core/modules/fastqc/main' addParams( options: modules['fastqc'] ) include { PYCOQC } from '../modules/nf-core/modules/pycoqc/main' addParams( options: modules['pycoqc'] ) -include { KRAKEN2_KRAKEN2 as KRAKEN2 } from '../modules/nf-core/modules/kraken2/kraken2/main' addParams( options: modules['kraken2'] ) +include { SAMTOOLS_SORT } from '../modules/nf-core/modules/samtools/sort/main' addParams( [publish_files : false] ) +include { SAMTOOLS_INDEX } from '../modules/nf-core/modules/samtools/index/main' addParams( [publish_files : false] ) +include { KRAKEN2_KRAKEN2 as KRAKEN2 } from '../modules/nf-core/modules/kraken2/kraken2/main' addParams( options: modules['kraken2'] ) include { QUAST } from '../modules/nf-core/modules/quast/main' addParams( options: modules['quast'] ) include { PROKKA } from '../modules/nf-core/modules/prokka/main' addParams( options: prokka_options ) include { MULTIQC } from '../modules/nf-core/modules/multiqc/main' addParams( options: multiqc_options ) @@ -239,8 +246,31 @@ workflow BACASS { // // MODULE: Nanopolish, polishes assembly using FAST5 files - should take either miniasm, canu, or unicycler consensus sequence // - //TODO - //when: !params.skip_polish && params.assembly_type == 'long' && params.polish_method != 'medaka' + if ( !params.skip_polish && params.assembly_type == 'long' && params.polish_method != 'medaka' ) { + ch_for_assembly + .join( ch_assembly ) + .set { ch_for_polish } + MINIMAP2_POLISH ( + ch_for_polish.dump(tag: 'into_minimap2_polish') + ) + ch_software_versions = ch_software_versions.mix(MINIMAP2_POLISH.out.version.first().ifEmpty(null)) + SAMTOOLS_SORT ( + MINIMAP2_POLISH.out.paf.map{ meta,sr,lr,ref,paf -> tuple(meta,paf) }.dump(tag: 'minimap2_polish') + ) + ch_software_versions = ch_software_versions.mix(SAMTOOLS_SORT.out.version.first().ifEmpty(null)) + SAMTOOLS_INDEX ( + SAMTOOLS_SORT.out.bam.dump(tag: 'samtools_sort') + ) + ch_software_versions = ch_software_versions.mix(SAMTOOLS_INDEX.out.version.first().ifEmpty(null)) + ch_for_polish //tuple val(meta), val(reads), file(longreads), file(assembly) + .join( SAMTOOLS_INDEX.out.bai ) //tuple val(meta), file(bai) + .join( INPUT_CHECK.out.fast5 ) //tuple val(meta), file(fast5) + .set { ch_for_nanopolish } //tuple val(meta), val(reads), file(longreads), file(assembly), file(bai), file(fast5) + NANOPOLISH ( + ch_for_nanopolish.dump(tag: 'into_nanopolish') + ) + ch_software_versions = ch_software_versions.mix(NANOPOLISH.out.version.first().ifEmpty(null)) + } // // MODULE: Medaka, polishes assembly - should take either miniasm, canu, or unicycler consensus sequence From a0df16f089b08c0a7c3a997461a7f4795c847352 Mon Sep 17 00:00:00 2001 From: d4straub Date: Mon, 16 Aug 2021 15:02:29 +0200 Subject: [PATCH 40/81] adjust resources --- conf/base.config | 20 +++++++++---------- modules/local/canu.nf | 3 +++ modules/local/medaka.nf | 3 +++ modules/local/miniasm.nf | 3 +++ modules/local/nanopolish.nf | 3 +++ modules/local/racon.nf | 3 +++ modules/local/unicycler.nf | 3 +++ .../nf-core/modules/kraken2/kraken2/main.nf | 2 ++ workflows/bacass.nf | 16 +++++++-------- 9 files changed, 37 insertions(+), 19 deletions(-) diff --git a/conf/base.config b/conf/base.config index afa50340..842ca37c 100644 --- a/conf/base.config +++ b/conf/base.config @@ -10,10 +10,9 @@ process { - // TODO nf-core: Check the defaults for all processes cpus = { check_max( 1 * task.attempt, 'cpus' ) } - memory = { check_max( 6.GB * task.attempt, 'memory' ) } - time = { check_max( 4.h * task.attempt, 'time' ) } + memory = { check_max( 1.GB * task.attempt, 'memory' ) } + time = { check_max( 1.h * task.attempt, 'time' ) } errorStrategy = { task.exitStatus in [143,137,104,134,139] ? 'retry' : 'finish' } maxRetries = 1 @@ -24,16 +23,15 @@ process { // These labels are used and recognised by default in DSL2 files hosted on nf-core/modules. // If possible, it would be nice to keep the same label naming convention when // adding in your local modules too. - // TODO nf-core: Customise requirements for specific processes. // See https://www.nextflow.io/docs/latest/config.html#config-process-selectors withLabel:process_low { cpus = { check_max( 2 * task.attempt, 'cpus' ) } - memory = { check_max( 12.GB * task.attempt, 'memory' ) } - time = { check_max( 4.h * task.attempt, 'time' ) } + memory = { check_max( 1.GB * task.attempt, 'memory' ) } + time = { check_max( 1.h * task.attempt, 'time' ) } } withLabel:process_medium { - cpus = { check_max( 6 * task.attempt, 'cpus' ) } - memory = { check_max( 36.GB * task.attempt, 'memory' ) } + cpus = { check_max( 8 * task.attempt, 'cpus' ) } + memory = { check_max( 8.GB * task.attempt, 'memory' ) } time = { check_max( 8.h * task.attempt, 'time' ) } } withLabel:process_high { @@ -42,16 +40,16 @@ process { time = { check_max( 16.h * task.attempt, 'time' ) } } withLabel:process_long { - time = { check_max( 20.h * task.attempt, 'time' ) } + time = { check_max( 160.h * task.attempt, 'time' ) } } withLabel:process_high_memory { - memory = { check_max( 200.GB * task.attempt, 'memory' ) } + memory = { check_max( 350.GB * task.attempt, 'memory' ) } } withLabel:error_ignore { errorStrategy = 'ignore' } withLabel:error_retry { errorStrategy = 'retry' - maxRetries = 2 + maxRetries = 3 } } diff --git a/modules/local/canu.nf b/modules/local/canu.nf index 016da8bf..09afecf6 100644 --- a/modules/local/canu.nf +++ b/modules/local/canu.nf @@ -7,6 +7,9 @@ options = initOptions(params.options) process CANU { tag "$meta.id" label 'process_high' + label 'process_long' + label 'process_high_memory' + label 'error_retry' publishDir "${params.outdir}", mode: params.publish_dir_mode, saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } diff --git a/modules/local/medaka.nf b/modules/local/medaka.nf index 6e8574e5..7af1e028 100644 --- a/modules/local/medaka.nf +++ b/modules/local/medaka.nf @@ -7,6 +7,9 @@ options = initOptions(params.options) process MEDAKA { tag "$meta.id" label 'process_high' + label 'process_long' + label 'process_high_memory' + label 'error_retry' publishDir "${params.outdir}", mode: params.publish_dir_mode, saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } diff --git a/modules/local/miniasm.nf b/modules/local/miniasm.nf index acfd298c..5a3cda7d 100644 --- a/modules/local/miniasm.nf +++ b/modules/local/miniasm.nf @@ -7,6 +7,9 @@ options = initOptions(params.options) process MINIASM { tag "$meta.id" label 'process_high' + label 'process_long' + label 'process_high_memory' + label 'error_retry' publishDir "${params.outdir}", mode: params.publish_dir_mode, saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } diff --git a/modules/local/nanopolish.nf b/modules/local/nanopolish.nf index d74bf451..607ec425 100644 --- a/modules/local/nanopolish.nf +++ b/modules/local/nanopolish.nf @@ -7,6 +7,9 @@ options = initOptions(params.options) process NANOPOLISH { tag "$meta.id" label 'process_high' + label 'process_long' + label 'process_high_memory' + label 'error_retry' publishDir "${params.outdir}", mode: params.publish_dir_mode, saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } diff --git a/modules/local/racon.nf b/modules/local/racon.nf index f1f1cad5..28973921 100644 --- a/modules/local/racon.nf +++ b/modules/local/racon.nf @@ -7,6 +7,9 @@ options = initOptions(params.options) process RACON { tag "$meta.id" label 'process_high' + label 'process_long' + label 'process_high_memory' + label 'error_retry' publishDir "${params.outdir}", mode: params.publish_dir_mode, saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } diff --git a/modules/local/unicycler.nf b/modules/local/unicycler.nf index e2cf4cec..83ce4703 100644 --- a/modules/local/unicycler.nf +++ b/modules/local/unicycler.nf @@ -7,6 +7,9 @@ options = initOptions(params.options) process UNICYCLER { tag "$meta.id" label 'process_high' + label 'process_long' + label 'process_high_memory' + label 'error_retry' publishDir "${params.outdir}", mode: params.publish_dir_mode, saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } diff --git a/modules/nf-core/modules/kraken2/kraken2/main.nf b/modules/nf-core/modules/kraken2/kraken2/main.nf index ea0b72fd..0fa86579 100644 --- a/modules/nf-core/modules/kraken2/kraken2/main.nf +++ b/modules/nf-core/modules/kraken2/kraken2/main.nf @@ -7,6 +7,8 @@ options = initOptions(params.options) process KRAKEN2_KRAKEN2 { tag "$meta.id" label 'process_high' + label 'process_long' + label 'process_high_memory' publishDir "${params.outdir}", mode: params.publish_dir_mode, saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } diff --git a/workflows/bacass.nf b/workflows/bacass.nf index 00dcbe37..1628af8c 100644 --- a/workflows/bacass.nf +++ b/workflows/bacass.nf @@ -140,7 +140,7 @@ workflow BACASS { SKEWER ( INPUT_CHECK.out.shortreads.dump(tag: 'shortreads') ) - ch_software_versions = ch_software_versions.mix(SKEWER.out.version.first().ifEmpty(null)) //TODO + ch_software_versions = ch_software_versions.mix(SKEWER.out.version.first().ifEmpty(null)) // // MODULE: Nanoplot, quality check for nanopore reads and Quality/Length Plots @@ -163,12 +163,12 @@ workflow BACASS { // // MODULE: PYCOQC, quality check for nanopore reads and Quality/Length Plots // - - // TODO: if ( params.assembly_type == 'hybrid' || params.assembly_type == 'long' && !('short' in params.assembly_type) ) - PORECHOP ( - INPUT_CHECK.out.longreads.dump(tag: 'longreads') - ) - ch_software_versions = ch_software_versions.mix(PORECHOP.out.version.first().ifEmpty(null)) + if ( params.assembly_type == 'hybrid' || params.assembly_type == 'long' && !('short' in params.assembly_type) ) { + PORECHOP ( + INPUT_CHECK.out.longreads.dump(tag: 'longreads') + ) + ch_software_versions = ch_software_versions.mix(PORECHOP.out.version.first().ifEmpty(null)) + } // // Join channels for assemblers. As samples have the same meta data, we can simply use join() to merge the channels based on this. If we only have one of the channels we insert 'NAs' which are not used in the unicycler process then subsequently, in case of short or long read only assembly. @@ -195,7 +195,7 @@ workflow BACASS { } // - // ASSEMBLY: Unicycler, Canu, Miniasm (TODO: convert into subworkflow) + // ASSEMBLY: Unicycler, Canu, Miniasm // ch_assembly = Channel.empty() From 00bc5fd8400daed68939e6cd61613b7f0cc2ae58 Mon Sep 17 00:00:00 2001 From: d4straub Date: Mon, 16 Aug 2021 16:12:48 +0200 Subject: [PATCH 41/81] fix test_long_miniasm --- conf/modules.config | 10 +++++++++- workflows/bacass.nf | 15 +++------------ 2 files changed, 12 insertions(+), 13 deletions(-) diff --git a/conf/modules.config b/conf/modules.config index 919cebf4..b5f388b8 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -51,7 +51,15 @@ params { publish_dir = "./Canu" } 'minimap_align' { - args = "" + args = "-x ava-ont" + publish_files = false + } + 'minimap_consensus' { + args = "-x map-ont" + publish_files = false + } + 'minimap_polish' { + args = "-ax map-ont" publish_files = false } 'miniasm' { diff --git a/workflows/bacass.nf b/workflows/bacass.nf index 1628af8c..fb9030df 100644 --- a/workflows/bacass.nf +++ b/workflows/bacass.nf @@ -50,15 +50,6 @@ unicycler_options.args += " $params.unicycler_args" def canu_options = modules['canu'] canu_options.args += " $params.canu_args" -def minimap_align_options = modules['minimap_align'] -minimap_align_options.args += " -x ava-ont" - -def minimap_consensus_options = modules['minimap_align'] -minimap_consensus_options.args += " -x map-ont" - -def minimap_polish_options = modules['minimap_align'] -minimap_polish_options.args += " -ax map-ont" - // // MODULE: Local to the pipeline // @@ -68,9 +59,9 @@ include { NANOPLOT } from '../modules/local/nanoplot' include { PORECHOP } from '../modules/local/porechop' addParams( options: modules['porechop'] ) include { UNICYCLER } from '../modules/local/unicycler' addParams( options: unicycler_options ) include { CANU } from '../modules/local/canu' addParams( options: canu_options ) -include { MINIMAP2_ALIGN } from '../modules/local/minimap_align' addParams( options: minimap_align_options ) -include { MINIMAP2_ALIGN as MINIMAP2_CONSENSUS } from '../modules/local/minimap_align' addParams( options: minimap_consensus_options) -include { MINIMAP2_ALIGN as MINIMAP2_POLISH } from '../modules/local/minimap_align' addParams( options: minimap_polish_options ) +include { MINIMAP2_ALIGN } from '../modules/local/minimap_align' addParams( options: modules['minimap_align'] ) +include { MINIMAP2_ALIGN as MINIMAP2_CONSENSUS } from '../modules/local/minimap_align' addParams( options: modules['minimap_consensus']) +include { MINIMAP2_ALIGN as MINIMAP2_POLISH } from '../modules/local/minimap_align' addParams( options: modules['minimap_polish']) include { MINIASM } from '../modules/local/miniasm' addParams( options: modules['miniasm'] ) include { RACON } from '../modules/local/racon' addParams( options: modules['racon'] ) include { MEDAKA } from '../modules/local/medaka' addParams( options: modules['medaka'] ) From 7d3fc0ab8a958667566a38ea758d8926e415d0ab Mon Sep 17 00:00:00 2001 From: d4straub Date: Mon, 16 Aug 2021 16:15:37 +0200 Subject: [PATCH 42/81] activate medaka for test_long_miniasm --- conf/test_long_miniasm.config | 1 - 1 file changed, 1 deletion(-) diff --git a/conf/test_long_miniasm.config b/conf/test_long_miniasm.config index c7719763..cc610324 100644 --- a/conf/test_long_miniasm.config +++ b/conf/test_long_miniasm.config @@ -26,6 +26,5 @@ params { prokka_args = " --fast" assembly_type = 'long' assembler = 'miniasm' - skip_polish = true skip_kraken2 = true } From 5b9153682b9d417ecc9ac86d89dd8039c06c4b61 Mon Sep 17 00:00:00 2001 From: d4straub Date: Tue, 17 Aug 2021 09:08:09 +0200 Subject: [PATCH 43/81] fix miniasm assembly --- workflows/bacass.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/workflows/bacass.nf b/workflows/bacass.nf index fb9030df..09f23288 100644 --- a/workflows/bacass.nf +++ b/workflows/bacass.nf @@ -230,7 +230,7 @@ workflow BACASS { RACON ( MINIMAP2_CONSENSUS.out.paf.dump(tag: 'minimap2_consensus') ) - ch_assembly = ch_assembly.mix( MINIASM.out.assembly.dump(tag: 'miniasm') ) + ch_assembly = ch_assembly.mix( RACON.out.assembly.dump(tag: 'miniasm') ) ch_software_versions = ch_software_versions.mix(RACON.out.version.first().ifEmpty(null)) } From 08fcfe8a26d32a8a780a076f50761476dab300a7 Mon Sep 17 00:00:00 2001 From: d4straub Date: Tue, 17 Aug 2021 10:02:03 +0200 Subject: [PATCH 44/81] fix kraken2 and activate for test_long_miniasm --- conf/test_long_miniasm.config | 2 +- modules/local/kraken2_db_preparation.nf | 31 +++++++++++++++++++++++++ modules/local/porechop.nf | 4 ++-- workflows/bacass.nf | 6 ++++- 4 files changed, 39 insertions(+), 4 deletions(-) create mode 100644 modules/local/kraken2_db_preparation.nf diff --git a/conf/test_long_miniasm.config b/conf/test_long_miniasm.config index cc610324..a68d3124 100644 --- a/conf/test_long_miniasm.config +++ b/conf/test_long_miniasm.config @@ -26,5 +26,5 @@ params { prokka_args = " --fast" assembly_type = 'long' assembler = 'miniasm' - skip_kraken2 = true + kraken2db = "https://genome-idx.s3.amazonaws.com/kraken/16S_Greengenes13.5_20200326.tgz" } diff --git a/modules/local/kraken2_db_preparation.nf b/modules/local/kraken2_db_preparation.nf new file mode 100644 index 00000000..559f4b2a --- /dev/null +++ b/modules/local/kraken2_db_preparation.nf @@ -0,0 +1,31 @@ +// Import generic module functions +include { initOptions; saveFiles; getSoftwareName } from './functions' + +params.options = [:] +options = initOptions(params.options) + +process KRAKEN2_DB_PREPARATION { + tag "${db.simpleName}" + label 'process_low' + + conda (params.enable_conda ? "conda-forge::sed=4.7" : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://containers.biocontainers.pro/s3/SingImgsRepo/biocontainers/v1.2.0_cv1/biocontainers_v1.2.0_cv1.img" + } else { + container "biocontainers/biocontainers:v1.2.0_cv1" + } + + input: + path db + + output: + tuple val("${db.simpleName}"), path("database"), emit: db + + script: + """ + mkdir db_tmp + tar -xf "${db}" -C db_tmp + mkdir database + mv `find db_tmp/ -name "*.k2d"` database/ + """ +} diff --git a/modules/local/porechop.nf b/modules/local/porechop.nf index 2ef954fb..9ebd7964 100644 --- a/modules/local/porechop.nf +++ b/modules/local/porechop.nf @@ -22,13 +22,13 @@ process PORECHOP { tuple val(meta), path(reads) output: - tuple val(meta), path('trimmed.fastq'), emit: reads + tuple val(meta), path('trimmed.fastq.gz'), emit: reads path "*.version.txt" , emit: version script: def software = getSoftwareName(task.process) """ - porechop $options.args -i "${reads}" -t "${task.cpus}" -o trimmed.fastq + porechop $options.args -i "${reads}" -t "${task.cpus}" -o trimmed.fastq.gz porechop --version > "${software}.version.txt" """ } diff --git a/workflows/bacass.nf b/workflows/bacass.nf index 09f23288..83d3e7e3 100644 --- a/workflows/bacass.nf +++ b/workflows/bacass.nf @@ -66,6 +66,7 @@ include { MINIASM } from '../modules/local/miniasm' include { RACON } from '../modules/local/racon' addParams( options: modules['racon'] ) include { MEDAKA } from '../modules/local/medaka' addParams( options: modules['medaka'] ) include { NANOPOLISH } from '../modules/local/nanopolish' addParams( options: modules['nanopolish'] ) +include { KRAKEN2_DB_PREPARATION} from '../modules/local/kraken2_db_preparation' include { DFAST } from '../modules/local/dfast' addParams( options: modules['dfast'] ) // @@ -286,9 +287,12 @@ workflow BACASS { [ meta, reads ] } .mix(SKEWER.out.reads) .set { ch_for_kraken2 } + KRAKEN2_DB_PREPARATION ( + kraken2db + ) KRAKEN2 ( ch_for_kraken2.dump(tag: 'kraken2'), - kraken2db + KRAKEN2_DB_PREPARATION.out.db.map { info, db -> db }.dump(tag: 'kraken2_db_preparation') ) ch_software_versions = ch_software_versions.mix(KRAKEN2.out.version.first().ifEmpty(null)) } From 206c6e69dae965d73dd1153bceda835aeba9f1c3 Mon Sep 17 00:00:00 2001 From: d4straub Date: Tue, 17 Aug 2021 15:18:58 +0200 Subject: [PATCH 45/81] adjust output folders --- conf/modules.config | 49 ++++++++++++++++++++++++++++++--------- modules/local/dfast.nf | 2 +- modules/local/porechop.nf | 2 +- modules/local/skewer.nf | 2 +- 4 files changed, 41 insertions(+), 14 deletions(-) diff --git a/conf/modules.config b/conf/modules.config index b5f388b8..efde96e2 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -24,73 +24,100 @@ params { modules { 'fastqc' { args = "--quiet" + publish_by_meta = ['id', 'trimming/shortreads'] + publish_dir = "." } 'skewer' { args = "-m pe -q 3 -n --quiet" - publish_dir = "./trimming/shortreads/" - publish_by_meta = true + publish_by_meta = ['id', 'trimming/shortreads'] + publish_dir = "." } 'nanoplot' { args = "" - publish_dir = "./QC_longreads/NanoPlot" + publish_by_meta = ['id', 'QC_longreads/NanoPlot'] + publish_dir = "." } 'pycoqc' { args = "" - publish_dir = "./QC_longreads/PycoQC" + publish_by_meta = ['id', 'QC_longreads/PycoQC'] + publish_dir = "." } 'porechop' { args = "" - publish_dir = "./trimming/longreads/" + publish_by_meta = ['id', 'trimming/longreads'] + publish_dir = "." } 'unicycler' { args = "" - publish_dir = "./Unicycler" + publish_by_meta = ['id', 'unicycler'] + publish_dir = "." } 'canu' { args = "" - publish_dir = "./Canu" + publish_by_meta = ['id', 'canu'] + publish_dir = "." } 'minimap_align' { args = "-x ava-ont" publish_files = false + publish_by_meta = ['id', 'minimap_align'] + publish_dir = "." } 'minimap_consensus' { args = "-x map-ont" publish_files = false + publish_by_meta = ['id', 'minimap_consensus'] + publish_dir = "." } 'minimap_polish' { args = "-ax map-ont" publish_files = false + publish_by_meta = ['id', 'minimap_polish'] + publish_dir = "." } 'miniasm' { args = "" publish_files = [ '_assembly.fasta':'' ] - publish_dir = "./Miniasm" + publish_by_meta = ['id', 'miniasm'] + publish_dir = "." } 'racon' { args = "" publish_files = [ '_assembly_consensus.fasta':'' ] - publish_dir = "./Miniasm/Racon" + publish_by_meta = ['id', 'miniasm'] + publish_dir = "." } 'medaka' { args = "" - publish_dir = "./Medaka" + publish_by_meta = ['id', 'medaka'] + publish_dir = "." } 'nanopolish' { args = "" - publish_dir = "./Nanopolish" + publish_by_meta = ['id', 'nanopolish'] + publish_dir = "." } 'kraken2' { args = "" + publish_files = [ '_kraken2.report':'' ] + publish_by_meta = ['id', 'kraken2'] + publish_dir = "." } 'quast' { args = "" + publish_by_meta = false //the module allows no meta, it collects all assemblies! + publish_dir = "./QUAST" + suffix = "other_files" } 'prokka' { args = "" + publish_by_meta = ['id', 'Prokka'] + publish_dir = "." } 'dfast' { args = "" + publish_by_meta = ['id', 'dfast'] + publish_dir = "." } 'multiqc' { args = "" diff --git a/modules/local/dfast.nf b/modules/local/dfast.nf index 0415ab13..bd515040 100644 --- a/modules/local/dfast.nf +++ b/modules/local/dfast.nf @@ -9,7 +9,7 @@ process DFAST { label 'process_medium' publishDir "${params.outdir}", mode: params.publish_dir_mode, - saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), publish_id:'') } + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } conda (params.enable_conda ? "dfast=1.2.14" : null) if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { diff --git a/modules/local/porechop.nf b/modules/local/porechop.nf index 9ebd7964..57a2cc2f 100644 --- a/modules/local/porechop.nf +++ b/modules/local/porechop.nf @@ -9,7 +9,7 @@ process PORECHOP { label 'process_medium' publishDir "${params.outdir}", mode: params.publish_dir_mode, - saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), publish_id:'') } + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } conda (params.enable_conda ? "porechop=0.2.4" : null) if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { diff --git a/modules/local/skewer.nf b/modules/local/skewer.nf index d2c79c7b..ac983b3b 100644 --- a/modules/local/skewer.nf +++ b/modules/local/skewer.nf @@ -9,7 +9,7 @@ process SKEWER { label 'process_medium' publishDir "${params.outdir}", mode: params.publish_dir_mode, - saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), publish_id:'') } + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } conda (params.enable_conda ? "skewer=0.2.2-3" : null) if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { From 82ba5a9de8594b56c8391d3d5cae4dd6673b8a88 Mon Sep 17 00:00:00 2001 From: d4straub Date: Wed, 18 Aug 2021 10:16:11 +0200 Subject: [PATCH 46/81] update CHANGELOG --- CHANGELOG.md | 21 ++++++++++++++++++++- 1 file changed, 20 insertions(+), 1 deletion(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index ffbf505a..0fe6b89e 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -5,14 +5,33 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 ## v2.0.0dev - [date] -Initial release of nf-core/bacass, created with the [nf-core](https://nf-co.re/) template. +### `Changed` + +* [#56](https://github.com/nf-core/bacass/pull/56) - Switched to DSL2 & update to new nf-core 2.1 `TEMPLATE` ### `Added` ### `Fixed` +* [#51](https://github.com/nf-core/bacass/issues/51) - Fixed Unicycler + ### `Dependencies` +* [#56](https://github.com/nf-core/bacass/pull/56) - Updated a bunch of dependencies (unchanged: FastQC, Miniasm, Prokka, Porechop, QUAST) + * Unicycler from 0.4.4 to 0.4.8 + * Kraken2 from 2.0.9beta to 2.1.1 + * MultiQC from 1.9 to 1.10.1 + * PYCOQC from 2.5.0.23 to 2.5.2 + * Samtools from 1.11 to 1.13 + * Canu from 2.0 to 2.1.1-2 + * dfast from 1.2.10 to 1.2.14 + * Medaka from 1.1.2 to 1.4.3-0 + * Minimap 2 from 2.17 to 2.21 + * Nanoplot from 1.32.1 to 1.38.0 + * Nanopolish from 0.13.2 to 0.13.2-5 + * Racon from 1.4.13 to 1.4.20-1 + * Skewer from 0.2.2 to 0.2.2-3 + ### `Deprecated` ## v1.1.1 nf-core/bacass: "Green Aluminium Shark" 2020/11/05 From 71e42dbfb18e8e2d56ccce3201f15a734c0e30a6 Mon Sep 17 00:00:00 2001 From: d4straub Date: Wed, 18 Aug 2021 10:16:34 +0200 Subject: [PATCH 47/81] fix kraken2 input --- workflows/bacass.nf | 26 +++++++++++++++++--------- 1 file changed, 17 insertions(+), 9 deletions(-) diff --git a/workflows/bacass.nf b/workflows/bacass.nf index 83d3e7e3..0676b0d5 100644 --- a/workflows/bacass.nf +++ b/workflows/bacass.nf @@ -164,6 +164,7 @@ workflow BACASS { // // Join channels for assemblers. As samples have the same meta data, we can simply use join() to merge the channels based on this. If we only have one of the channels we insert 'NAs' which are not used in the unicycler process then subsequently, in case of short or long read only assembly. + // Prepare channel for Kraken2 // if(params.assembly_type == 'hybrid'){ PORECHOP.out.reads.dump(tag: 'porechop') @@ -172,18 +173,33 @@ workflow BACASS { .join(PORECHOP.out.reads) .dump(tag: 'ch_for_assembly') .set { ch_for_assembly } + PORECHOP.out.reads + .map { info, reads -> + def meta = info + meta.single_end = true + [ meta, reads ] } + .mix(SKEWER.out.reads) + .set { ch_for_kraken2 } } else if ( params.assembly_type == 'short' ) { SKEWER.out.reads .dump(tag: 'skewer') .map{ meta,reads -> tuple(meta,reads,'NA') } .dump(tag: 'ch_for_assembly') .set { ch_for_assembly } + SKEWER.out.reads + .set { ch_for_kraken2 } } else if ( params.assembly_type == 'long' ) { PORECHOP.out.reads .dump(tag: 'porechop') .map{ meta,lr -> tuple(meta,'NA',lr) } .dump(tag: 'ch_for_assembly') - .set { ch_for_assembly } //old channel name: ch_short_long_joint_unicycler + .set { ch_for_assembly } + PORECHOP.out.reads + .map { info, reads -> + def meta = info + meta.single_end = true + [ meta, reads ] } + .set { ch_for_kraken2 } } // @@ -279,14 +295,6 @@ workflow BACASS { // MODULE: Kraken2, QC for sample purity // if ( !params.skip_kraken2 ) { - //prepare channel that specifies "meta.single_end = true" for long reads - PORECHOP.out.reads - .map { info, reads -> - def meta = info - meta.single_end = true - [ meta, reads ] } - .mix(SKEWER.out.reads) - .set { ch_for_kraken2 } KRAKEN2_DB_PREPARATION ( kraken2db ) From df6a5bd3b2876cd343241b96238d74878b7904de Mon Sep 17 00:00:00 2001 From: d4straub Date: Wed, 18 Aug 2021 10:39:27 +0200 Subject: [PATCH 48/81] fix kraken2 output --- conf/modules.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/conf/modules.config b/conf/modules.config index efde96e2..b1952707 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -99,7 +99,7 @@ params { } 'kraken2' { args = "" - publish_files = [ '_kraken2.report':'' ] + publish_files = [ 'report.txt':'' ] publish_by_meta = ['id', 'kraken2'] publish_dir = "." } From aefcd1e96de61082f50ab7b58e1033223d7ce8f1 Mon Sep 17 00:00:00 2001 From: d4straub Date: Wed, 18 Aug 2021 16:12:29 +0200 Subject: [PATCH 49/81] fix kraken2 output 2 --- conf/modules.config | 7 +++++++ workflows/bacass.nf | 37 ++++++++++++++++++++----------------- 2 files changed, 27 insertions(+), 17 deletions(-) diff --git a/conf/modules.config b/conf/modules.config index b1952707..0ca9cbb8 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -103,6 +103,13 @@ params { publish_by_meta = ['id', 'kraken2'] publish_dir = "." } + 'kraken2_long' { + args = "" + suffix = "_longreads" + publish_files = [ 'report.txt':'' ] + publish_by_meta = ['id', 'kraken2'] + publish_dir = "." + } 'quast' { args = "" publish_by_meta = false //the module allows no meta, it collects all assemblies! diff --git a/workflows/bacass.nf b/workflows/bacass.nf index 0676b0d5..193261ab 100644 --- a/workflows/bacass.nf +++ b/workflows/bacass.nf @@ -94,6 +94,7 @@ include { PYCOQC } from '../modules/nf-core/modules/pycoqc/main' add include { SAMTOOLS_SORT } from '../modules/nf-core/modules/samtools/sort/main' addParams( [publish_files : false] ) include { SAMTOOLS_INDEX } from '../modules/nf-core/modules/samtools/index/main' addParams( [publish_files : false] ) include { KRAKEN2_KRAKEN2 as KRAKEN2 } from '../modules/nf-core/modules/kraken2/kraken2/main' addParams( options: modules['kraken2'] ) +include { KRAKEN2_KRAKEN2 as KRAKEN2_LONG } from '../modules/nf-core/modules/kraken2/kraken2/main' addParams( options: modules['kraken2_long'] ) include { QUAST } from '../modules/nf-core/modules/quast/main' addParams( options: modules['quast'] ) include { PROKKA } from '../modules/nf-core/modules/prokka/main' addParams( options: prokka_options ) include { MULTIQC } from '../modules/nf-core/modules/multiqc/main' addParams( options: multiqc_options ) @@ -167,39 +168,29 @@ workflow BACASS { // Prepare channel for Kraken2 // if(params.assembly_type == 'hybrid'){ - PORECHOP.out.reads.dump(tag: 'porechop') + ch_for_kraken2_short = SKEWER.out.reads + ch_for_kraken2_long = PORECHOP.out.reads.dump(tag: 'porechop') SKEWER.out.reads .dump(tag: 'skewer') .join(PORECHOP.out.reads) .dump(tag: 'ch_for_assembly') .set { ch_for_assembly } - PORECHOP.out.reads - .map { info, reads -> - def meta = info - meta.single_end = true - [ meta, reads ] } - .mix(SKEWER.out.reads) - .set { ch_for_kraken2 } } else if ( params.assembly_type == 'short' ) { + ch_for_kraken2_short = SKEWER.out.reads + ch_for_kraken2_long = Channel.empty() SKEWER.out.reads .dump(tag: 'skewer') .map{ meta,reads -> tuple(meta,reads,'NA') } .dump(tag: 'ch_for_assembly') .set { ch_for_assembly } - SKEWER.out.reads - .set { ch_for_kraken2 } } else if ( params.assembly_type == 'long' ) { + ch_for_kraken2_short = Channel.empty() + ch_for_kraken2_long = PORECHOP.out.reads PORECHOP.out.reads .dump(tag: 'porechop') .map{ meta,lr -> tuple(meta,'NA',lr) } .dump(tag: 'ch_for_assembly') .set { ch_for_assembly } - PORECHOP.out.reads - .map { info, reads -> - def meta = info - meta.single_end = true - [ meta, reads ] } - .set { ch_for_kraken2 } } // @@ -299,10 +290,22 @@ workflow BACASS { kraken2db ) KRAKEN2 ( - ch_for_kraken2.dump(tag: 'kraken2'), + ch_for_kraken2_short.dump(tag: 'kraken2_short'), KRAKEN2_DB_PREPARATION.out.db.map { info, db -> db }.dump(tag: 'kraken2_db_preparation') ) ch_software_versions = ch_software_versions.mix(KRAKEN2.out.version.first().ifEmpty(null)) + KRAKEN2_LONG ( + ch_for_kraken2_long + .map { meta, reads -> + info = [:] + info.id = meta.id + info.single_end = true + [ info, reads ] + } + .dump(tag: 'kraken2_long'), + KRAKEN2_DB_PREPARATION.out.db.map { info, db -> db }.dump(tag: 'kraken2_db_preparation') + ) + ch_software_versions = ch_software_versions.mix(KRAKEN2_LONG.out.version.first().ifEmpty(null)) } // From e3a071fe337a90ee9ccb510793c89dcb47c9e8d8 Mon Sep 17 00:00:00 2001 From: d4straub Date: Wed, 18 Aug 2021 16:17:51 +0200 Subject: [PATCH 50/81] fix linting --- workflows/bacass.nf | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/workflows/bacass.nf b/workflows/bacass.nf index 193261ab..044297ae 100644 --- a/workflows/bacass.nf +++ b/workflows/bacass.nf @@ -299,8 +299,8 @@ workflow BACASS { .map { meta, reads -> info = [:] info.id = meta.id - info.single_end = true - [ info, reads ] + info.single_end = true + [ info, reads ] } .dump(tag: 'kraken2_long'), KRAKEN2_DB_PREPARATION.out.db.map { info, db -> db }.dump(tag: 'kraken2_db_preparation') From 54ead7ffacf84068db23f21556d7211e7b003e18 Mon Sep 17 00:00:00 2001 From: d4straub Date: Wed, 18 Aug 2021 17:19:55 +0200 Subject: [PATCH 51/81] refine output folders --- conf/modules.config | 18 +++++++++--------- 1 file changed, 9 insertions(+), 9 deletions(-) diff --git a/conf/modules.config b/conf/modules.config index 0ca9cbb8..05e753c2 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -24,7 +24,7 @@ params { modules { 'fastqc' { args = "--quiet" - publish_by_meta = ['id', 'trimming/shortreads'] + publish_by_meta = ['id', 'FastQC'] publish_dir = "." } 'skewer' { @@ -49,12 +49,12 @@ params { } 'unicycler' { args = "" - publish_by_meta = ['id', 'unicycler'] + publish_by_meta = ['id', 'Unicycler'] publish_dir = "." } 'canu' { args = "" - publish_by_meta = ['id', 'canu'] + publish_by_meta = ['id', 'Canu'] publish_dir = "." } 'minimap_align' { @@ -78,36 +78,36 @@ params { 'miniasm' { args = "" publish_files = [ '_assembly.fasta':'' ] - publish_by_meta = ['id', 'miniasm'] + publish_by_meta = ['id', 'Miniasm'] publish_dir = "." } 'racon' { args = "" publish_files = [ '_assembly_consensus.fasta':'' ] - publish_by_meta = ['id', 'miniasm'] + publish_by_meta = ['id', 'Miniasm'] publish_dir = "." } 'medaka' { args = "" - publish_by_meta = ['id', 'medaka'] + publish_by_meta = ['id', 'Medaka'] publish_dir = "." } 'nanopolish' { args = "" - publish_by_meta = ['id', 'nanopolish'] + publish_by_meta = ['id', 'Nanopolish'] publish_dir = "." } 'kraken2' { args = "" publish_files = [ 'report.txt':'' ] - publish_by_meta = ['id', 'kraken2'] + publish_by_meta = ['id', 'Kraken2'] publish_dir = "." } 'kraken2_long' { args = "" suffix = "_longreads" publish_files = [ 'report.txt':'' ] - publish_by_meta = ['id', 'kraken2'] + publish_by_meta = ['id', 'Kraken2'] publish_dir = "." } 'quast' { From 05b54d0b4ff6a232f4f314870ee965a5b08f06dd Mon Sep 17 00:00:00 2001 From: d4straub Date: Thu, 19 Aug 2021 11:24:46 +0200 Subject: [PATCH 52/81] add change in --krakendb --- CHANGELOG.md | 1 + 1 file changed, 1 insertion(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index 0fe6b89e..f8afad75 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -8,6 +8,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 ### `Changed` * [#56](https://github.com/nf-core/bacass/pull/56) - Switched to DSL2 & update to new nf-core 2.1 `TEMPLATE` +* [#56](https://github.com/nf-core/bacass/pull/56) - `--krakendb` now expects a `.tar.gz`/`.tgz` (compressed tar archive) directly from `https://benlangmead.github.io/aws-indexes/k2` instead of an uncompressed folder. ### `Added` From 1f8718c066331221f34fca31fb6e0d4bfb388ec5 Mon Sep 17 00:00:00 2001 From: d4straub Date: Thu, 19 Aug 2021 11:43:54 +0200 Subject: [PATCH 53/81] add zenodo doi --- lib/WorkflowMain.groovy | 5 ++--- 1 file changed, 2 insertions(+), 3 deletions(-) diff --git a/lib/WorkflowMain.groovy b/lib/WorkflowMain.groovy index 6e61e6a5..d2827006 100755 --- a/lib/WorkflowMain.groovy +++ b/lib/WorkflowMain.groovy @@ -9,9 +9,8 @@ class WorkflowMain { // public static String citation(workflow) { return "If you use ${workflow.manifest.name} for your analysis please cite:\n\n" + - // TODO nf-core: Add Zenodo DOI for pipeline after first release - //"* The pipeline\n" + - //" https://doi.org/10.5281/zenodo.XXXXXXX\n\n" + + "* The pipeline\n" + + " https://doi.org/10.5281/zenodo.2669428\n\n" + "* The nf-core framework\n" + " https://doi.org/10.1038/s41587-020-0439-x\n\n" + "* Software dependencies\n" + From a58fd8e021173f2bbc1897583d0231457df237dc Mon Sep 17 00:00:00 2001 From: d4straub Date: Thu, 19 Aug 2021 13:55:56 +0200 Subject: [PATCH 54/81] change dfast output to folder DFAST --- conf/modules.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/conf/modules.config b/conf/modules.config index 05e753c2..a27a1c5d 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -121,7 +121,7 @@ params { publish_by_meta = ['id', 'Prokka'] publish_dir = "." } - 'dfast' { + 'DFAST' { args = "" publish_by_meta = ['id', 'dfast'] publish_dir = "." From 05ac341b9ad0c1a2e04e8a8d673be7ad8cc3e6f5 Mon Sep 17 00:00:00 2001 From: d4straub Date: Thu, 19 Aug 2021 13:56:30 +0200 Subject: [PATCH 55/81] update CITATTIONS --- CITATIONS.md | 37 +++++++++++++++++++++++++++++++++++++ 1 file changed, 37 insertions(+) diff --git a/CITATIONS.md b/CITATIONS.md index 6f36eb1e..5e061325 100644 --- a/CITATIONS.md +++ b/CITATIONS.md @@ -12,6 +12,43 @@ * [FastQC](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/) +* [Skewer](https://pubmed.ncbi.nlm.nih.gov/24925680/) + > Jiang H, Lei R, Ding SW, Zhu S. Skewer: a fast and accurate adapter trimmer for next-generation sequencing paired-end reads. BMC Bioinformatics. 2014 Jun 12;15:182. doi: 10.1186/1471-2105-15-182. PMID: 24925680; PMCID: PMC4074385. + +* [Porechop](https://github.com/rrwick/Porechop) + +* [NanoPlot](https://doi.org/10.1093/bioinformatics/bty149) + > De Coster, W., D’Hert, S., Schultz, D. T., Cruts, M., & Van Broeckhoven, C. (2018). NanoPack: visualizing and processing long-read sequencing data. Bioinformatics, 34(15), 2666-2669. doi: 10.1093/bioinformatics/bty149. + +* [pycoQC](https://github.com/tleonardi/pycoQC) + +* [Unicycler](https://pubmed.ncbi.nlm.nih.gov/28594827/) + > Wick RR, Judd LM, Gorrie CL, Holt KE. Unicycler: Resolving bacterial genome assemblies from short and long sequencing reads. PLoS Comput Biol. 2017 Jun 8;13(6):e1005595. doi: 10.1371/journal.pcbi.1005595. PMID: 28594827; PMCID: PMC5481147. + +* [Miniasm](https://github.com/lh3/miniasm) with [Racon](https://github.com/isovic/racon) + +* [Canu](https://pubmed.ncbi.nlm.nih.gov/28298431/) + > Koren S, Walenz BP, Berlin K, Miller JR, Bergman NH, Phillippy AM. Canu: scalable and accurate long-read assembly via adaptive k-mer weighting and repeat separation. Genome Res. 2017 May;27(5):722-736. doi: 10.1101/gr.215087.116. Epub 2017 Mar 15. PMID: 28298431; PMCID: PMC5411767. + +* [QUAST](https://pubmed.ncbi.nlm.nih.gov/23422339/) + > Gurevich A, Saveliev V, Vyahhi N, Tesler G. QUAST: quality assessment tool for genome assemblies. Bioinformatics. 2013 Apr 15;29(8):1072-5. doi: 10.1093/bioinformatics/btt086. Epub 2013 Feb 19. PMID: 23422339; PMCID: PMC3624806. + +* [Prokka](https://pubmed.ncbi.nlm.nih.gov/24642063/) + > Seemann T. Prokka: rapid prokaryotic genome annotation. Bioinformatics. 2014 Jul 15;30(14):2068-9. doi: 10.1093/bioinformatics/btu153. Epub 2014 Mar 18. PMID: 24642063. + +* [DFAST](https://pubmed.ncbi.nlm.nih.gov/29106469/) + > Tanizawa Y, Fujisawa T, Nakamura Y. DFAST: a flexible prokaryotic genome annotation pipeline for faster genome publication. Bioinformatics. 2018 Mar 15;34(6):1037-1039. doi: 10.1093/bioinformatics/btx713. PMID: 29106469; PMCID: PMC5860143. + +* [Medaka](https://github.com/nanoporetech/medaka) + +* [Nanopolish](https://github.com/jts/nanopolish) + +* [SAMtools](https://doi.org/10.1093/bioinformatics/btp352) + > Li, H., Handsaker, B., Wysoker, A., Fennell, T., Ruan, J., Homer, N., … 1000 Genome Project Data Processing Subgroup. (2009). The Sequence Alignment/Map format and SAMtools. Bioinformatics , 25(16), 2078–2079. doi: 10.1093/bioinformatics/btp352. + +* [Kraken2](https://doi.org/10.1186/s13059-019-1891-0) + > Wood, D et al., 2019. Improved metagenomic analysis with Kraken 2. Genome Biology volume 20, Article number: 257. doi: 10.1186/s13059-019-1891-0. + * [MultiQC](https://www.ncbi.nlm.nih.gov/pubmed/27312411/) > Ewels P, Magnusson M, Lundin S, Käller M. MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics. 2016 Oct 1;32(19):3047-8. doi: 10.1093/bioinformatics/btw354. Epub 2016 Jun 16. PubMed PMID: 27312411; PubMed Central PMCID: PMC5039924. From 5091db831be8feb7bb7cff8d60bba2d258988cae Mon Sep 17 00:00:00 2001 From: d4straub Date: Thu, 19 Aug 2021 13:57:13 +0200 Subject: [PATCH 56/81] update README --- README.md | 19 ++++++++++++++----- 1 file changed, 14 insertions(+), 5 deletions(-) diff --git a/README.md b/README.md index 574aacc5..23860045 100644 --- a/README.md +++ b/README.md @@ -32,12 +32,16 @@ This pipeline is primarily for bacterial assembly of next-generation sequencing ### Long Read Assembly For users that only have Nanopore data, the pipeline quality trims these using [PoreChop](https://github.com/rrwick/Porechop) and assesses basic sequencing QC utilizing [NanoPlot](https://github.com/wdecoster/NanoPlot) and [PycoQC](https://github.com/a-slide/pycoQC). -The pipeline can then perform long read assembly utilizing [Unicycler](https://github.com/rrwick/Unicycler), [Miniasm](https://github.com/lh3/miniasm) in combination with [Racon](https://github.com/isovic/racon) or [Canu](https://github.com/marbl/canu). Long reads can be polished using specified Fast5 files with [NanoPolish](https://github.com/jts/nanopolish). +The pipeline can then perform long read assembly utilizing [Unicycler](https://github.com/rrwick/Unicycler), [Miniasm](https://github.com/lh3/miniasm) in combination with [Racon](https://github.com/isovic/racon), or [Canu](https://github.com/marbl/canu). Long reads assembly can be polished using [Medaka](https://github.com/nanoporetech/medaka) or [NanoPolish](https://github.com/jts/nanopolish) with Fast5 files. ### Hybrid Assembly For users specifying both short read and long read (NanoPore) data, the pipeline can perform a hybrid assembly approach utilizing [Unicycler](https://github.com/rrwick/Unicycler), taking the full set of information from short reads and long reads into account. +### Assembly QC and annotation + +In all cases, the assembly is assessed using [QUAST](http://bioinf.spbau.ru/quast). The resulting bacterial assembly is furthermore annotated using [Prokka](https://github.com/tseemann/prokka) or [DFAST](https://github.com/nigyta/dfast_core). + ## Quick Start 1. Install [`Nextflow`](https://www.nextflow.io/docs/latest/getstarted.html#installation) (`>=21.04.0`) @@ -56,10 +60,16 @@ For users specifying both short read and long read (NanoPore) data, the pipeline 4. Start running your own analysis! - + Default: Short read assembly with Unicycler, `--kraken2db` can be any [compressed database (`.tar.gz`/`.tgz`)](https://benlangmead.github.io/aws-indexes/k2): + + ```console + nextflow run nf-core/bacass -profile --input samplesheet.tsv --kraken2db "https://genome-idx.s3.amazonaws.com/kraken/k2_standard_8gb_20210517.tar.gz" + ``` + + Long read assembly with Miniasm: ```console - nextflow run nf-core/bacass -profile --input samplesheet.csv --genome GRCh37 + nextflow run nf-core/bacass -profile --input samplesheet.tsv --assembly_type 'long' --assembler 'miniasm' --kraken2db "https://genome-idx.s3.amazonaws.com/kraken/k2_standard_8gb_20210517.tar.gz" ``` ## Documentation @@ -72,7 +82,7 @@ nf-core/bacass was originally written by Andreas Wilm, Alexander Peltzer. We thank the following people for their extensive assistance in the development of this pipeline: - +* Daniel Straub ([@d4straub](https://github.com/d4straub)) ## Contributions and Support @@ -84,7 +94,6 @@ For further information or help, don't hesitate to get in touch on the [Slack `# If you use nf-core/bacass for your analysis, please cite it using the following doi: [10.5281/zenodo.2669428](https://doi.org/10.5281/zenodo.2669428) - An extensive list of references for the tools used by the pipeline can be found in the [`CITATIONS.md`](CITATIONS.md) file. You can cite the `nf-core` publication as follows: From d6b9840796737a518a5a5753140fe302b42900e3 Mon Sep 17 00:00:00 2001 From: d4straub Date: Thu, 19 Aug 2021 13:57:36 +0200 Subject: [PATCH 57/81] update output docs --- docs/output.md | 190 ++++++++++++++++++++++++++++++++++++++++++++----- 1 file changed, 174 insertions(+), 16 deletions(-) diff --git a/docs/output.md b/docs/output.md index 2f830a01..b060dbcf 100644 --- a/docs/output.md +++ b/docs/output.md @@ -12,49 +12,209 @@ The directories listed below will be created in the results directory after the The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes data using the following steps: -* [FastQC](#fastqc) - Raw read QC -* [MultiQC](#multiqc) - Aggregate report describing results and QC from the whole pipeline +* [Quality trimming and QC](#quality-trimming-and-qc) + * [Short Read Trimming](#short-read-trimming) + * [Short Read RAW QC](#short-read-raw-qc) + * [Long Read Trimming](#long-read-trimming) + * [Long Read RAW QC](#long-read-raw-qc) +* [Taxonomic classification](#taxonomic-classification) +* [Assembly Output](#assembly-output) +* [Assembly QC with QUAST](#assembly-qc-with-quast) +* [Annotation with Prokka](#annotation-with-prokka) +* [Annotation with DFAST](#annotation-with-dfast) +* [Report](#report) * [Pipeline information](#pipeline-information) - Report metrics generated during the workflow execution -### FastQC +## Quality trimming and QC + +### Short Read Trimming + +This step quality trims the end of reads, removes degenerate or too short reads and if needed, +combines reads coming from multiple sequencing runs. + +
+Output files + +* `{sample_id}/trimming/shortreads/` + * `*.fastq.gz`: Trimmed (and combined reads) + +
+ +### Short Read RAW QC + +[FastQC](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/) gives general quality metrics about your sequenced reads. It provides information about the quality score distribution across your reads, per base sequence content (%A/T/G/C), adapter contamination and overrepresented sequences. For further reading and documentation see the [FastQC help pages](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/Help/). + +> **NB:** The FastQC plots displayed in the MultiQC report shows _untrimmed_ reads. They may contain adapter sequence and potentially regions with low quality.
Output files -* `fastqc/` +* `{sample_id}/FastQC/` * `*_fastqc.html`: FastQC report containing quality metrics. * `*_fastqc.zip`: Zip archive containing the FastQC report, tab-delimited data file and plot images. +![FastQC report](images/fastqc.png) +
-[FastQC](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/) gives general quality metrics about your sequenced reads. It provides information about the quality score distribution across your reads, per base sequence content (%A/T/G/C), adapter contamination and overrepresented sequences. For further reading and documentation see the [FastQC help pages](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/Help/). +### Long Read Trimming -![MultiQC - FastQC sequence counts plot](images/mqc_fastqc_counts.png) +This step performs long read trimming on Nanopore input (if provided). -![MultiQC - FastQC mean quality scores plot](images/mqc_fastqc_quality.png) +
+Output files -![MultiQC - FastQC adapter content plot](images/mqc_fastqc_adapter.png) +* `{sample_id}/trimming/longreads/` + * `trimmed.fastq.gz`: The trimmed FASTQ file -> **NB:** The FastQC plots displayed in the MultiQC report shows _untrimmed_ reads. They may contain adapter sequence and potentially regions with low quality. +
+ +### Long Read RAW QC + +These steps perform long read QC for input data (if provided). -### MultiQC +Please refer to the documentation of [NanoPlot](https://github.com/wdecoster/NanoPlot) and [PycoQC](https://a-slide.github.io/pycoQC/) if you want to know more about the plots created by these tools.
Output files -* `multiqc/` - * `multiqc_report.html`: a standalone HTML file that can be viewed in your web browser. - * `multiqc_data/`: directory containing parsed statistics from the different tools used in the pipeline. - * `multiqc_plots/`: directory containing static images from the report in various formats. +* `{sample_id}/QC_Longreads/` + * `NanoPlot`: Various plots in HTML and PNG format + * `PycoQC` + +Example plot from Nanoplot: + +![Nanoplot](images/nanoplot.png) + +
+ +## Taxonomic classification + +This QC step classifies your reads using [Kraken2](https://ccb.jhu.edu/software/kraken2/) a k-mer based approach. This helps to identify samples that have purity +issues. Ideally you will not want to assemble reads from samples that are contaminated or contain +multiple species. If you like to visualize the report, try +[Pavian](https://github.com/fbreitwieser/pavian) or [Krakey](http://krakey.info/). + +
+Output files + +* `{sample}/Kraken2` + * `{sample}.kraken2.report.txt`: Classification of short reads in the Kraken(1) report format. + * `{sample}_longreads.kraken2.report.txt`: Classification of long reads in the Kraken(1) report format. + +See [webpage](http://ccb.jhu.edu/software/kraken/MANUAL.html#sample-reports) for more details. + +Exemplary Kraken2 report screenshot: + +![Kraken2 report](images/kraken2.png)
+## Assembly Output + +Trimmed reads are assembled with [Unicycler](https://github.com/rrwick/Unicycler) in `short` or `hybrid` assembly modes. For long-read assembly, there are also `canu` and `miniasm` available. +Unicycler is a pipeline on its own, which at least for Illumina reads mainly acts as a frontend to Spades with added polishing steps. + +
+Output files + +* `{sample_id}/Unicycler` + * `{sample}.scaffolds.fa`: Final assembly in fasta format + * `{sample}.assembly.gfa`: Final assembly in Graphical Fragment Assembly (GFA) format + * `{sample}.unicycler.log`: Log file summarizing steps and intermediate results on the Unicycler execution + +Check out the [Unicycler documentation](https://github.com/rrwick/Unicycler) for more information on Unicycler output. + +* `{sample_id}/Canu` + * `{sample}_assembly.fasta`: Final assembly in fasta format + * `{sample}_assembly.report`: Log file + +Check out the [Canu documentation](https://canu.readthedocs.io/en/latest/index.html) for more information on Canu output. + +* `{sample_id}/Miniasm` + * `{sample}_assembly.fasta`: Assembly in fasta format + * `{sample}_assembly_consensus.fasta`: Consensus assembly in fasta format (polished by Racon) + +Check out the [Miniasm documentation](https://github.com/lh3/miniasm) for more information on Miniasm output. + +
+ +## Assembly QC with QUAST + +The assembly QC is performed with [QUAST](http://quast.sourceforge.net/quast) for all assemblies in one report. It reports multiple metrics including number of contigs, N50, lengths etc in form of an html report. It further creates an HTML file with integrated contig viewer (Icarus). + +
+Output files + +* `QUAST` + * `report.tsv`: QUAST's report in text format +* `QUAST/other_files` + * `icarus.html`: QUAST's contig browser as HTML + * `report.html`: QUAST assembly QC as HTML report + * `report.pdf`: QUAST assembly QC as pdf + +![QUAST QC](images/quast.png) + +![Icarus](images/icarus.png) + +
+ +## Annotation with Prokka + +By default, the assembly is annotated with [Prokka](https://github.com/tseemann/prokka) which acts as frontend for several annotation tools and includes rRNA and ORF predictions. + +
+Output files + +* `{sample_id}/Prokka/{sample_id}` + * `{sample_id}.gff`: Annotation in gff format + * `{sample_id}.txt`: Annotation in text format + * `{sample_id}.faa`: Protein sequences in fasta format + +See [Prokka's documentation](https://github.com/tseemann/prokka#output-files) for a full description of all output files. + +![Prokka annotation](images/prokka.png) + +
+ +## Annotation with DFAST + +On request, the assembly is annotated with [DFAST](https://github.com/nigyta/dfast_core). + +
+Output files + +* `{sample_id}/DFAST/RESULT_{dfast_profile_name}` + * `genome.gff`: Annotation in gff format + * `statistics.txt`: Annotation statistics in text format + * `protein.faa`: Protein sequences in fasta format + +
+ +## Report + +Some pipeline results are visualised by [MultiQC](http://multiqc.info), which is a visualisation tool that generates a single HTML report summarising all samples in your project. Further statistics are available in within the report data directory. + [MultiQC](http://multiqc.info) is a visualization tool that generates a single HTML report summarising all samples in your project. Most of the pipeline QC results are visualised in the report and further statistics are available in the report data directory. +The pipeline has special steps which also allow the software versions to be reported in the MultiQC output for future traceability. + Results generated by MultiQC collate pipeline QC from supported tools e.g. FastQC. The pipeline has special steps which also allow the software versions to be reported in the MultiQC output for future traceability. For more information about how to use MultiQC reports, see . +
+Output files + +* `multiqc/` + * `multiqc_report.html`: a standalone HTML file that can be viewed in your web browser. + * `multiqc_data/`: directory containing parsed statistics from the different tools used in the pipeline. + * `multiqc_plots/`: directory containing static images from the report in various formats. + +
+ ### Pipeline information +[Nextflow](https://www.nextflow.io/docs/latest/tracing.html) provides excellent functionality for generating various reports relevant to the running and execution of the pipeline. This will allow you to troubleshoot errors with the running of the pipeline, and also provide you with other information such as launch commands, run times and resource usage. +
Output files @@ -64,5 +224,3 @@ Results generated by MultiQC collate pipeline QC from supported tools e.g. FastQ * Reformatted samplesheet files used as input to the pipeline: `samplesheet.valid.csv`.
- -[Nextflow](https://www.nextflow.io/docs/latest/tracing.html) provides excellent functionality for generating various reports relevant to the running and execution of the pipeline. This will allow you to troubleshoot errors with the running of the pipeline, and also provide you with other information such as launch commands, run times and resource usage. From 34fc91792656a919a7b3083545391e3c8dbad49e Mon Sep 17 00:00:00 2001 From: d4straub Date: Thu, 19 Aug 2021 14:17:13 +0200 Subject: [PATCH 58/81] remove TODO --- docs/output.md | 2 -- 1 file changed, 2 deletions(-) diff --git a/docs/output.md b/docs/output.md index b060dbcf..9b060f0a 100644 --- a/docs/output.md +++ b/docs/output.md @@ -6,8 +6,6 @@ This document describes the output produced by the pipeline. Most of the plots a The directories listed below will be created in the results directory after the pipeline has finished. All paths are relative to the top-level results directory. - - ## Pipeline overview The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes data using the following steps: From ad96a766cbae943d400de47deb9194a34f972172 Mon Sep 17 00:00:00 2001 From: d4straub Date: Thu, 19 Aug 2021 14:26:26 +0200 Subject: [PATCH 59/81] fix dfast --- conf/modules.config | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/conf/modules.config b/conf/modules.config index a27a1c5d..01d7d2c6 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -121,9 +121,9 @@ params { publish_by_meta = ['id', 'Prokka'] publish_dir = "." } - 'DFAST' { + 'dfast' { args = "" - publish_by_meta = ['id', 'dfast'] + publish_by_meta = ['id', 'DFAST'] publish_dir = "." } 'multiqc' { From 9c0b316d3d8ef050445e15c13203bc5e059a168e Mon Sep 17 00:00:00 2001 From: d4straub Date: Thu, 19 Aug 2021 14:27:55 +0200 Subject: [PATCH 60/81] fix picoqc --- modules.json | 3 - .../pycoqc/main.nf => local/pycoqc.nf} | 22 ++++-- modules/nf-core/modules/pycoqc/functions.nf | 68 ------------------- modules/nf-core/modules/pycoqc/meta.yml | 48 ------------- workflows/bacass.nf | 2 +- 5 files changed, 18 insertions(+), 125 deletions(-) rename modules/{nf-core/modules/pycoqc/main.nf => local/pycoqc.nf} (59%) delete mode 100644 modules/nf-core/modules/pycoqc/functions.nf delete mode 100644 modules/nf-core/modules/pycoqc/meta.yml diff --git a/modules.json b/modules.json index 1103fbc5..2ef7020a 100644 --- a/modules.json +++ b/modules.json @@ -15,9 +15,6 @@ "prokka": { "git_sha": "e937c7950af70930d1f34bb961403d9d2aa81c7d" }, - "pycoqc": { - "git_sha": "e937c7950af70930d1f34bb961403d9d2aa81c7d" - }, "quast": { "git_sha": "e937c7950af70930d1f34bb961403d9d2aa81c7d" }, diff --git a/modules/nf-core/modules/pycoqc/main.nf b/modules/local/pycoqc.nf similarity index 59% rename from modules/nf-core/modules/pycoqc/main.nf rename to modules/local/pycoqc.nf index 3f010247..36109769 100644 --- a/modules/nf-core/modules/pycoqc/main.nf +++ b/modules/local/pycoqc.nf @@ -9,7 +9,7 @@ process PYCOQC { label 'process_medium' publishDir "${params.outdir}", mode: params.publish_dir_mode, - saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:[:], publish_by_meta:[]) } + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } conda (params.enable_conda ? "bioconda::pycoqc=2.5.2" : null) if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { @@ -19,21 +19,33 @@ process PYCOQC { } input: - path summary + tuple val(meta), path(fast5) output: + tuple val(meta), path('sequencing_summary.txt'), emit: summary path "*.html" , emit: html path "*.json" , emit: json path "*.version.txt", emit: version script: def software = getSoftwareName(task.process) + //Find out whether the sequencing_summary already exists + if(file("${fast5}/sequencing_summary.txt").exists()){ + run_summary = "cp ${fast5}/sequencing_summary.txt ./sequencing_summary.txt" + } else { + run_summary = "Fast5_to_seq_summary -f $fast5 -t ${task.cpus} -s './sequencing_summary.txt' --verbose_level 2" + } + //Barcodes available? + barcode_me = file("${fast5}/barcoding_sequencing.txt").exists() ? "-b ${fast5}/barcoding_sequencing.txt" : '' """ + $run_summary + pycoQC \\ $options.args \\ - -f $summary \\ - -o pycoqc.html \\ - -j pycoqc.json + -f "sequencing_summary.txt" \\ + $barcode_me + -o ${sample_id}_pycoqc.html \\ + -j ${sample_id}_pycoqc.json echo \$(pycoQC --version 2>&1) | sed 's/^.*pycoQC v//; s/ .*\$//' > ${software}.version.txt """ diff --git a/modules/nf-core/modules/pycoqc/functions.nf b/modules/nf-core/modules/pycoqc/functions.nf deleted file mode 100644 index da9da093..00000000 --- a/modules/nf-core/modules/pycoqc/functions.nf +++ /dev/null @@ -1,68 +0,0 @@ -// -// Utility functions used in nf-core DSL2 module files -// - -// -// Extract name of software tool from process name using $task.process -// -def getSoftwareName(task_process) { - return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() -} - -// -// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules -// -def initOptions(Map args) { - def Map options = [:] - options.args = args.args ?: '' - options.args2 = args.args2 ?: '' - options.args3 = args.args3 ?: '' - options.publish_by_meta = args.publish_by_meta ?: [] - options.publish_dir = args.publish_dir ?: '' - options.publish_files = args.publish_files - options.suffix = args.suffix ?: '' - return options -} - -// -// Tidy up and join elements of a list to return a path string -// -def getPathFromList(path_list) { - def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries - paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes - return paths.join('/') -} - -// -// Function to save/publish module results -// -def saveFiles(Map args) { - if (!args.filename.endsWith('.version.txt')) { - def ioptions = initOptions(args.options) - def path_list = [ ioptions.publish_dir ?: args.publish_dir ] - if (ioptions.publish_by_meta) { - def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta - for (key in key_list) { - if (args.meta && key instanceof String) { - def path = key - if (args.meta.containsKey(key)) { - path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] - } - path = path instanceof String ? path : '' - path_list.add(path) - } - } - } - if (ioptions.publish_files instanceof Map) { - for (ext in ioptions.publish_files) { - if (args.filename.endsWith(ext.key)) { - def ext_list = path_list.collect() - ext_list.add(ext.value) - return "${getPathFromList(ext_list)}/$args.filename" - } - } - } else if (ioptions.publish_files == null) { - return "${getPathFromList(path_list)}/$args.filename" - } - } -} diff --git a/modules/nf-core/modules/pycoqc/meta.yml b/modules/nf-core/modules/pycoqc/meta.yml deleted file mode 100644 index 059b2f15..00000000 --- a/modules/nf-core/modules/pycoqc/meta.yml +++ /dev/null @@ -1,48 +0,0 @@ -name: pycoqc -description: write your description here -keywords: - - qc - - quality control - - sequencing - - nanopore -tools: - - pycoqc: - description: PycoQC computes metrics and generates interactive QC plots for Oxford Nanopore technologies sequencing data - homepage: https://github.com/tleonardi/pycoQC - documentation: https://tleonardi.github.io/pycoQC/ - tool_dev_url: https://github.com/tleonardi/pycoQC - doi: "10.21105/joss.01236" - licence: ['GNU General Public v3 (GPL v3)'] - -input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - summary: - type: file - description: sequencing summary file - pattern: "*.{txt}" - -output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - html: - type: file - description: Results in HTML format - - json: - type: file - description: Results in JSON format - pattern: "*.{json}" - - version: - type: file - description: File containing software version - pattern: "*.{version.txt}" - -authors: - - "@joseespinosa" - - "@drpatelh" diff --git a/workflows/bacass.nf b/workflows/bacass.nf index 044297ae..2ea4bdf3 100644 --- a/workflows/bacass.nf +++ b/workflows/bacass.nf @@ -56,6 +56,7 @@ canu_options.args += " $params.canu_args" include { GET_SOFTWARE_VERSIONS } from '../modules/local/get_software_versions' addParams( options: [publish_files : ['tsv':'']] ) include { SKEWER } from '../modules/local/skewer' addParams( options: modules['skewer'] ) include { NANOPLOT } from '../modules/local/nanoplot' addParams( options: modules['nanoplot'] ) +include { PYCOQC } from '../modules/local/pycoqc' addParams( options: modules['pycoqc'] ) include { PORECHOP } from '../modules/local/porechop' addParams( options: modules['porechop'] ) include { UNICYCLER } from '../modules/local/unicycler' addParams( options: unicycler_options ) include { CANU } from '../modules/local/canu' addParams( options: canu_options ) @@ -90,7 +91,6 @@ prokka_options.args += " $params.prokka_args" // MODULE: Installed directly from nf-core/modules // include { FASTQC } from '../modules/nf-core/modules/fastqc/main' addParams( options: modules['fastqc'] ) -include { PYCOQC } from '../modules/nf-core/modules/pycoqc/main' addParams( options: modules['pycoqc'] ) include { SAMTOOLS_SORT } from '../modules/nf-core/modules/samtools/sort/main' addParams( [publish_files : false] ) include { SAMTOOLS_INDEX } from '../modules/nf-core/modules/samtools/index/main' addParams( [publish_files : false] ) include { KRAKEN2_KRAKEN2 as KRAKEN2 } from '../modules/nf-core/modules/kraken2/kraken2/main' addParams( options: modules['kraken2'] ) From 911186a8d4136dc0926651b713af8d0125630e86 Mon Sep 17 00:00:00 2001 From: d4straub Date: Thu, 19 Aug 2021 14:31:44 +0200 Subject: [PATCH 61/81] fix linting and pycoqc --- docs/output.md | 6 +++--- modules/local/pycoqc.nf | 6 +++--- 2 files changed, 6 insertions(+), 6 deletions(-) diff --git a/docs/output.md b/docs/output.md index 9b060f0a..33115c84 100644 --- a/docs/output.md +++ b/docs/output.md @@ -97,9 +97,9 @@ multiple species. If you like to visualize the report, try Output files * `{sample}/Kraken2` - * `{sample}.kraken2.report.txt`: Classification of short reads in the Kraken(1) report format. - * `{sample}_longreads.kraken2.report.txt`: Classification of long reads in the Kraken(1) report format. - + * `{sample}.kraken2.report.txt`: Classification of short reads in the Kraken(1) report format. + * `{sample}_longreads.kraken2.report.txt`: Classification of long reads in the Kraken(1) report format. + See [webpage](http://ccb.jhu.edu/software/kraken/MANUAL.html#sample-reports) for more details. Exemplary Kraken2 report screenshot: diff --git a/modules/local/pycoqc.nf b/modules/local/pycoqc.nf index 36109769..3c2167c0 100644 --- a/modules/local/pycoqc.nf +++ b/modules/local/pycoqc.nf @@ -35,7 +35,7 @@ process PYCOQC { } else { run_summary = "Fast5_to_seq_summary -f $fast5 -t ${task.cpus} -s './sequencing_summary.txt' --verbose_level 2" } - //Barcodes available? + //Barcodes available? barcode_me = file("${fast5}/barcoding_sequencing.txt").exists() ? "-b ${fast5}/barcoding_sequencing.txt" : '' """ $run_summary @@ -44,8 +44,8 @@ process PYCOQC { $options.args \\ -f "sequencing_summary.txt" \\ $barcode_me - -o ${sample_id}_pycoqc.html \\ - -j ${sample_id}_pycoqc.json + -o ${meta.id}_pycoqc.html \\ + -j ${meta.id}_pycoqc.json echo \$(pycoQC --version 2>&1) | sed 's/^.*pycoQC v//; s/ .*\$//' > ${software}.version.txt """ From 097938e1e87247eb03ae7d400b0d9fb08878738a Mon Sep 17 00:00:00 2001 From: d4straub Date: Thu, 19 Aug 2021 16:52:46 +0200 Subject: [PATCH 62/81] update output with medaka and nanopolish --- docs/output.md | 20 ++++++++++++++++++++ 1 file changed, 20 insertions(+) diff --git a/docs/output.md b/docs/output.md index 33115c84..1cf9c639 100644 --- a/docs/output.md +++ b/docs/output.md @@ -17,6 +17,7 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d * [Long Read RAW QC](#long-read-raw-qc) * [Taxonomic classification](#taxonomic-classification) * [Assembly Output](#assembly-output) + * [Polished assemblies](#polished-assemblies) * [Assembly QC with QUAST](#assembly-qc-with-quast) * [Annotation with Prokka](#annotation-with-prokka) * [Annotation with DFAST](#annotation-with-dfast) @@ -137,6 +138,25 @@ Check out the [Miniasm documentation](https://github.com/lh3/miniasm) for more i +### Polished assemblies + +Long reads assemblies can be polished using [Medaka](https://github.com/nanoporetech/medaka) or [NanoPolish](https://github.com/jts/nanopolish) with Fast5 files. + +
+Output files + +* `{sample_id}/Medaka/{sample_id}_polished_genome.fa` + * `consensus.fasta`: Polished consensus assembly in fasta format + * `calls_to_draft.bam`: Alignment in bam format + * `calls_to_draft.bam.bai`: Index of alignment + * `consensus.fasta.gaps_in_draft_coords.bed` + * `consensus_probs.hdf` + +* `{sample_id}/Nanopolish` + * `polished_genome.fa`: Polished consensus assembly in fasta format + +
+ ## Assembly QC with QUAST The assembly QC is performed with [QUAST](http://quast.sourceforge.net/quast) for all assemblies in one report. It reports multiple metrics including number of contigs, N50, lengths etc in form of an html report. It further creates an HTML file with integrated contig viewer (Icarus). From 5ecd08e437498d2bf2364a19c116755c9c9e9202 Mon Sep 17 00:00:00 2001 From: d4straub Date: Thu, 19 Aug 2021 16:53:43 +0200 Subject: [PATCH 63/81] remove samtools files from results folder --- workflows/bacass.nf | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/workflows/bacass.nf b/workflows/bacass.nf index 2ea4bdf3..f4f1cd2d 100644 --- a/workflows/bacass.nf +++ b/workflows/bacass.nf @@ -91,8 +91,8 @@ prokka_options.args += " $params.prokka_args" // MODULE: Installed directly from nf-core/modules // include { FASTQC } from '../modules/nf-core/modules/fastqc/main' addParams( options: modules['fastqc'] ) -include { SAMTOOLS_SORT } from '../modules/nf-core/modules/samtools/sort/main' addParams( [publish_files : false] ) -include { SAMTOOLS_INDEX } from '../modules/nf-core/modules/samtools/index/main' addParams( [publish_files : false] ) +include { SAMTOOLS_SORT } from '../modules/nf-core/modules/samtools/sort/main' addParams( options: [publish_files : false] ) +include { SAMTOOLS_INDEX } from '../modules/nf-core/modules/samtools/index/main' addParams( options: [publish_files : false] ) include { KRAKEN2_KRAKEN2 as KRAKEN2 } from '../modules/nf-core/modules/kraken2/kraken2/main' addParams( options: modules['kraken2'] ) include { KRAKEN2_KRAKEN2 as KRAKEN2_LONG } from '../modules/nf-core/modules/kraken2/kraken2/main' addParams( options: modules['kraken2_long'] ) include { QUAST } from '../modules/nf-core/modules/quast/main' addParams( options: modules['quast'] ) From e7b9ec24fe6b85d38053f5de9fcf87167be6ffe8 Mon Sep 17 00:00:00 2001 From: d4straub Date: Thu, 19 Aug 2021 16:54:32 +0200 Subject: [PATCH 64/81] update parameter descriptions --- nextflow_schema.json | 23 +++++++++++++---------- 1 file changed, 13 insertions(+), 10 deletions(-) diff --git a/nextflow_schema.json b/nextflow_schema.json index cf597abc..356a5d7d 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -21,7 +21,7 @@ "pattern": "^\\S+\\.csv$", "schema": "assets/schema_input.json", "description": "Path to comma-separated file containing information about the samples in the experiment.", - "help_text": "You will need to create a design file with information about the samples in your experiment before running the pipeline. Use this parameter to specify its location. It has to be a tab-separated file with 6 columns, and a header row. See [usage docs](https://nf-co.re/bacass/usage#samplesheet-input).\n\nFor example:\n\n`--input 'design_hybrid.csv'`\n\nAn example of properly formatted input files can be found at the [nf-core/testData](https://github.com/nf-core/test-datasets/tree/bacass). \n\nExemplarily, this is the input used for a hybrid assembly in testing:\nID R1 R2 LongFastQ Fast5 GenomeSize\nERR044595 https://github.com/nf-core/test-datasets/raw/bacass/ERR044595_1M_1.fastq.gz https://github.com/nf-core/test-datasets/raw/bacass/ERR044595_1M_2.fastq.gz https://github.com/nf-core/test-datasets/raw/bacass/nanopore/subset15000.fq.gz NA 2.8m\n\n* `ID`: The identifier to use for handling the dataset e.g. sample name\n* `R1`: The forward reads in case of available short-read data\n* `R2`: The reverse reads in case of available short-read data\n* `LongFastQ`: The long read FastQ file with reads in FASTQ format\n* `Fast5`: The folder containing the basecalled fast5 files\n* `GenomeSize`: The expected genome size of the assembly. Only used by the canu assembler.\n\nMissing values (e.g. Fast5 folder in case of short reads) can be omitted by using a `NA` in the TSV file. The pipeline will handle such cases appropriately then.", + "help_text": "You will need to create a design file with information about the samples in your experiment before running the pipeline. Use this parameter to specify its location. It has to be a tab-separated file with 6 columns, and a header row. See [usage docs](https://nf-co.re/bacass/usage#samplesheet-input).\n\nFor example:\n\n`--input 'design_hybrid.csv'`\n\nAn example of properly formatted input files can be found at the [nf-core/test-datasets](https://github.com/nf-core/test-datasets/tree/bacass). \n\nFor example, this is the input used for a hybrid assembly in testing:\nID R1 R2 LongFastQ Fast5 GenomeSize\nERR044595 https://github.com/nf-core/test-datasets/raw/bacass/ERR044595_1M_1.fastq.gz https://github.com/nf-core/test-datasets/raw/bacass/ERR044595_1M_2.fastq.gz https://github.com/nf-core/test-datasets/raw/bacass/nanopore/subset15000.fq.gz NA 2.8m\n\n* `ID`: The identifier to use for handling the dataset e.g. sample name\n* `R1`: The forward reads in case of available short-read data\n* `R2`: The reverse reads in case of available short-read data\n* `LongFastQ`: The long read FastQ file with reads in FASTQ format\n* `Fast5`: The folder containing the basecalled fast5 files\n* `GenomeSize`: The expected genome size of the assembly. Only used by the canu assembler.\n\nMissing values (e.g. Fast5 folder in case of short reads) can be omitted by using a `NA` in the TSV file. The pipeline will handle such cases appropriately then.", "fa_icon": "fas fa-file-csv" }, "outdir": { @@ -36,11 +36,6 @@ "fa_icon": "fas fa-envelope", "help_text": "Set this parameter to your e-mail address to get a summary e-mail with details of the run sent to you when the workflow exits. If set in your user config file (`~/.nextflow/config`) then you don't need to specify this on the command line for every run.", "pattern": "^([a-zA-Z0-9_\\-\\.]+)@([a-zA-Z0-9_\\-\\.]+)\\.([a-zA-Z]{2,5})$" - }, - "multiqc_title": { - "type": "string", - "description": "MultiQC report title. Printed as page header, used for filename if not otherwise specified.", - "fa_icon": "fas fa-file-signature" } } }, @@ -127,8 +122,9 @@ }, "dfast_config": { "type": "string", - "default": "/Users/alexanderpeltzer/IDEA/nf-core/bacass/assets/test_config_dfast.py", - "description": "Specifies a configuration file for the [DFAST](https://github.com/nigyta/dfast_core) annotation method. This can be used instead of PROKKA if required to specify a specific config file for annotation. If you want to know how to create your config file, please refer to the [DFAST](https://github.com/nigyta/dfast_core) readme on how to create one. > The default config is just included for testing, so if you want to annotate using DFAST, you have to create a config!" + "default": "assets/test_config_dfast.py", + "description": "Specifies a configuration file for the [DFAST](https://github.com/nigyta/dfast_core) annotation method.", + "help_text": "This can be used instead of PROKKA if required to specify a specific config file for annotation. If you want to know how to create your config file, please refer to the [DFAST](https://github.com/nigyta/dfast_core) readme on how to create one. The default config (`assets/test_config_dfast.py`) is just included for testing, so if you want to annotate using DFAST, you have to create a config!" } } }, @@ -171,7 +167,8 @@ "type": "string", "description": "Name of iGenomes reference.", "fa_icon": "fas fa-book", - "help_text": "If using a reference genome configured in the pipeline using iGenomes, use this parameter to give the ID for the reference. This is then used to build the full paths for all required reference genome files e.g. `--genome GRCh38`. \n\nSee the [nf-core website docs](https://nf-co.re/usage/reference_genomes) for more details." + "help_text": "If using a reference genome configured in the pipeline using iGenomes, use this parameter to give the ID for the reference. This is then used to build the full paths for all required reference genome files e.g. `--genome GRCh38`. \n\nSee the [nf-core website docs](https://nf-co.re/usage/reference_genomes) for more details.", + "hidden": true }, "fasta": { "type": "string", @@ -180,7 +177,8 @@ "pattern": "^\\S+\\.fn?a(sta)?(\\.gz)?$", "description": "Path to FASTA genome file.", "help_text": "This parameter is *mandatory* if `--genome` is not specified. If you don't have a BWA index available this will be generated for you automatically. Combine with `--save_reference` to save BWA index for future runs.", - "fa_icon": "far fa-file-code" + "fa_icon": "far fa-file-code", + "hidden": true }, "igenomes_base": { "type": "string", @@ -317,6 +315,11 @@ ], "hidden": true }, + "multiqc_title": { + "type": "string", + "description": "MultiQC report title. Printed as page header, used for filename if not otherwise specified.", + "fa_icon": "fas fa-file-signature" + }, "email_on_fail": { "type": "string", "description": "Email address for completion summary, only when pipeline fails.", From 545763a791214fad8d03f7bb9b5a63777214afaf Mon Sep 17 00:00:00 2001 From: d4straub Date: Fri, 20 Aug 2021 09:57:07 +0200 Subject: [PATCH 65/81] remove parallel from nanopolish --- modules/local/nanopolish.nf | 11 +++-------- 1 file changed, 3 insertions(+), 8 deletions(-) diff --git a/modules/local/nanopolish.nf b/modules/local/nanopolish.nf index 607ec425..ee579bd6 100644 --- a/modules/local/nanopolish.nf +++ b/modules/local/nanopolish.nf @@ -34,21 +34,16 @@ process NANOPOLISH { """ nanopolish index -d "${fast5}" "${longreads}" - nanopolish_makerange.py "${assembly}" | \ - parallel \ - --results nanopolish.results \ - -P "${task.cpus}" \ - nanopolish variants \ + nanopolish variants \ --consensus \ - -o polished.{1}.vcf \ - -w {1} \ + -o polished.vcf \ -r "${longreads}" \ -b "${prefix}.bam" \ -g "${assembly}" \ -t "${task.cpus}" \ --min-candidate-frequency 0.1 - nanopolish vcf2fasta -g "${assembly}" polished.*.vcf > polished_genome.fa + nanopolish vcf2fasta -g "${assembly}" polished.vcf > polished_genome.fa nanopolish --version | sed -e "s/nanopolish version //g" | head -n 1 > ${software}.version.txt """ From 6c6f01ac9fd75b38139df5e776d0e5d58580ff1b Mon Sep 17 00:00:00 2001 From: d4straub Date: Fri, 20 Aug 2021 10:17:21 +0200 Subject: [PATCH 66/81] fix pycoqc --- conf/modules.config | 1 + modules/local/pycoqc.nf | 4 ++-- 2 files changed, 3 insertions(+), 2 deletions(-) diff --git a/conf/modules.config b/conf/modules.config index 01d7d2c6..17944342 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -39,6 +39,7 @@ params { } 'pycoqc' { args = "" + publish_files = [ '.html':'', '.json':'' ] publish_by_meta = ['id', 'QC_longreads/PycoQC'] publish_dir = "." } diff --git a/modules/local/pycoqc.nf b/modules/local/pycoqc.nf index 3c2167c0..8c0de668 100644 --- a/modules/local/pycoqc.nf +++ b/modules/local/pycoqc.nf @@ -5,7 +5,7 @@ params.options = [:] options = initOptions(params.options) process PYCOQC { - tag "$summary" + tag "$meta.id" label 'process_medium' publishDir "${params.outdir}", mode: params.publish_dir_mode, @@ -43,7 +43,7 @@ process PYCOQC { pycoQC \\ $options.args \\ -f "sequencing_summary.txt" \\ - $barcode_me + $barcode_me \\ -o ${meta.id}_pycoqc.html \\ -j ${meta.id}_pycoqc.json From 787ae4570cb790beb12b5cd4eb1367a998a795fe Mon Sep 17 00:00:00 2001 From: d4straub Date: Fri, 20 Aug 2021 10:44:54 +0200 Subject: [PATCH 67/81] amend output and update usage --- assets/samplesheet.csv | 7 ++++--- docs/output.md | 24 ++++++++-------------- docs/usage.md | 46 +++++++++++++++++------------------------- 3 files changed, 30 insertions(+), 47 deletions(-) diff --git a/assets/samplesheet.csv b/assets/samplesheet.csv index 5f653ab7..983c7011 100644 --- a/assets/samplesheet.csv +++ b/assets/samplesheet.csv @@ -1,3 +1,4 @@ -sample,fastq_1,fastq_2 -SAMPLE_PAIRED_END,/path/to/fastq/files/AEG588A1_S1_L002_R1_001.fastq.gz,/path/to/fastq/files/AEG588A1_S1_L002_R2_001.fastq.gz -SAMPLE_SINGLE_END,/path/to/fastq/files/AEG588A4_S4_L003_R1_001.fastq.gz, +ID R1 R2 LongFastQ Fast5 GenomeSize +shortreads ./data/S1_R1.fastq.gz ./data/S1_R2.fastq.gz NA NA NA +longreads NA NA ./data/S1_long_fastq.gz ./data/FAST5 2.8m +shortNlong ./data/S1_R1.fastq.gz ./data/S1_R2.fastq.gz ./data/S1_long_fastq.gz ./data/FAST5 2.8m diff --git a/docs/output.md b/docs/output.md index 1cf9c639..974f9e22 100644 --- a/docs/output.md +++ b/docs/output.md @@ -19,8 +19,7 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d * [Assembly Output](#assembly-output) * [Polished assemblies](#polished-assemblies) * [Assembly QC with QUAST](#assembly-qc-with-quast) -* [Annotation with Prokka](#annotation-with-prokka) -* [Annotation with DFAST](#annotation-with-dfast) +* [Annotation](#annotation) * [Report](#report) * [Pipeline information](#pipeline-information) - Report metrics generated during the workflow execution @@ -77,9 +76,11 @@ Please refer to the documentation of [NanoPlot](https://github.com/wdecoster/Nan
Output files -* `{sample_id}/QC_Longreads/` - * `NanoPlot`: Various plots in HTML and PNG format - * `PycoQC` +* `{sample_id}/QC_Longreads/NanoPlot`: Various plots in HTML and PNG format + +* `{sample_id}/QC_Longreads/PycoQC` + * `{sample_id}_pycoqc.html`: QC report in HTML format + * `{sample_id}_pycoqc.json`: QC report in JSON format Example plot from Nanoplot: @@ -177,9 +178,9 @@ The assembly QC is performed with [QUAST](http://quast.sourceforge.net/quast) fo
-## Annotation with Prokka +## Annotation -By default, the assembly is annotated with [Prokka](https://github.com/tseemann/prokka) which acts as frontend for several annotation tools and includes rRNA and ORF predictions. +By default, the assembly is annotated with [Prokka](https://github.com/tseemann/prokka) which acts as frontend for several annotation tools and includes rRNA and ORF predictions. Alternatively, on request, the assembly is annotated with [DFAST](https://github.com/nigyta/dfast_core).
Output files @@ -193,15 +194,6 @@ See [Prokka's documentation](https://github.com/tseemann/prokka#output-files) fo ![Prokka annotation](images/prokka.png) -
- -## Annotation with DFAST - -On request, the assembly is annotated with [DFAST](https://github.com/nigyta/dfast_core). - -
-Output files - * `{sample_id}/DFAST/RESULT_{dfast_profile_name}` * `genome.gff`: Annotation in gff format * `statistics.txt`: Annotation statistics in text format diff --git a/docs/usage.md b/docs/usage.md index b50bca2f..1a0c1184 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -16,39 +16,29 @@ You will need to create a samplesheet with information about the samples you wou --input '[path to samplesheet file]' ``` -### Multiple runs of the same sample +### Samplesheet -The `sample` identifiers have to be the same when you have re-sequenced the same sample more than once e.g. to increase sequencing depth. The pipeline will concatenate the raw reads before performing any downstream analysis. Below is an example for the same sample sequenced across 3 lanes: +The samplesheet must have 6 columns defined in the table below. -```console -sample,fastq_1,fastq_2 -CONTROL_REP1,AEG588A1_S1_L002_R1_001.fastq.gz,AEG588A1_S1_L002_R2_001.fastq.gz -CONTROL_REP1,AEG588A1_S1_L003_R1_001.fastq.gz,AEG588A1_S1_L003_R2_001.fastq.gz -CONTROL_REP1,AEG588A1_S1_L004_R1_001.fastq.gz,AEG588A1_S1_L004_R2_001.fastq.gz -``` - -### Full samplesheet - -The pipeline will auto-detect whether a sample is single- or paired-end using the information provided in the samplesheet. The samplesheet can have as many columns as you desire, however, there is a strict requirement for the first 3 columns to match those defined in the table below. - -A final samplesheet file consisting of both single- and paired-end data may look something like the one below. This is for 6 samples, where `TREATMENT_REP3` has been sequenced twice. +A final samplesheet file consisting of short read, long reads, or short and long reads. This is for 3 samples. ```console -sample,fastq_1,fastq_2 -CONTROL_REP1,AEG588A1_S1_L002_R1_001.fastq.gz,AEG588A1_S1_L002_R2_001.fastq.gz -CONTROL_REP2,AEG588A2_S2_L002_R1_001.fastq.gz,AEG588A2_S2_L002_R2_001.fastq.gz -CONTROL_REP3,AEG588A3_S3_L002_R1_001.fastq.gz,AEG588A3_S3_L002_R2_001.fastq.gz -TREATMENT_REP1,AEG588A4_S4_L003_R1_001.fastq.gz, -TREATMENT_REP2,AEG588A5_S5_L003_R1_001.fastq.gz, -TREATMENT_REP3,AEG588A6_S6_L003_R1_001.fastq.gz, -TREATMENT_REP3,AEG588A6_S6_L004_R1_001.fastq.gz, +ID R1 R2 LongFastQ Fast5 GenomeSize +shortreads ./data/S1_R1.fastq.gz ./data/S1_R2.fastq.gz NA NA NA +longreads NA NA ./data/S1_long_fastq.gz ./data/FAST5 2.8m +shortNlong ./data/S1_R1.fastq.gz ./data/S1_R2.fastq.gz ./data/S1_long_fastq.gz ./data/FAST5 2.8m ``` -| Column | Description | -|----------------|----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------| -| `sample` | Custom sample name. This entry will be identical for multiple sequencing libraries/runs from the same sample. Spaces in sample names are automatically converted to underscores (`_`). | -| `fastq_1` | Full path to FastQ file for Illumina short reads 1. File has to be gzipped and have the extension ".fastq.gz" or ".fq.gz". | -| `fastq_2` | Full path to FastQ file for Illumina short reads 2. File has to be gzipped and have the extension ".fastq.gz" or ".fq.gz". | +> **NB:** `./data/FAST5` points at a folder containing all (i.e. one or mutiple) fast5 files that correspond to the long reads. `NA` indicates that the file is missing. + +| Column | Description | +|-|-| +| `sample` | Custom sample name. May not contain spaces. | +| `R1` | Full path to FastQ file for Illumina short reads 1. File has to be gzipped and have the extension ".fastq.gz" or ".fq.gz". `NA` indicates that the file is missing. | +| `R2` | Full path to FastQ file for Illumina short reads 2. File has to be gzipped and have the extension ".fastq.gz" or ".fq.gz". `NA` indicates that the file is missing. | +| `LongFastQ` | Full path to FastQ file for ONT long reads. File has to be gzipped and have the extension ".fastq.gz" or ".fq.gz". `NA` indicates that the file is missing. | +| `Fast5` | Full path to a folder containing Fast5 file(s) for ONT long reads. `NA` indicates that there are no Fast5 files available. | +| `GenomeSize` | Expected genome size. For example, `2.8m` means 2.8 million basepairs genome size expected. This is only used by Canu assembler. `NA` indicates that this value is unknown. | An [example samplesheet](../assets/samplesheet.csv) has been provided with the pipeline. @@ -57,7 +47,7 @@ An [example samplesheet](../assets/samplesheet.csv) has been provided with the p The typical command for running the pipeline is as follows: ```console -nextflow run nf-core/bacass --input samplesheet.csv --genome GRCh37 -profile docker +nextflow run nf-core/bacass --input samplesheet.csv -profile docker --skip_kraken2 ``` This will launch the pipeline with the `docker` configuration profile. See below for more information about profiles. From 1df85e51905af7d3c0616a28298251db0969dba4 Mon Sep 17 00:00:00 2001 From: d4straub Date: Fri, 20 Aug 2021 10:51:19 +0200 Subject: [PATCH 68/81] remove tabs --- docs/usage.md | 8 ++++---- 1 file changed, 4 insertions(+), 4 deletions(-) diff --git a/docs/usage.md b/docs/usage.md index 1a0c1184..b3407e36 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -23,10 +23,10 @@ The samplesheet must have 6 columns defined in the table below. A final samplesheet file consisting of short read, long reads, or short and long reads. This is for 3 samples. ```console -ID R1 R2 LongFastQ Fast5 GenomeSize -shortreads ./data/S1_R1.fastq.gz ./data/S1_R2.fastq.gz NA NA NA -longreads NA NA ./data/S1_long_fastq.gz ./data/FAST5 2.8m -shortNlong ./data/S1_R1.fastq.gz ./data/S1_R2.fastq.gz ./data/S1_long_fastq.gz ./data/FAST5 2.8m +ID R1 R2 LongFastQ Fast5 GenomeSize +shortreads ./data/S1_R1.fastq.gz ./data/S1_R2.fastq.gz NA NA NA +longreads NA NA ./data/S1_long_fastq.gz ./data/FAST5 2.8m +shortNlong ./data/S1_R1.fastq.gz ./data/S1_R2.fastq.gz ./data/S1_long_fastq.gz ./data/FAST5 2.8m ``` > **NB:** `./data/FAST5` points at a folder containing all (i.e. one or mutiple) fast5 files that correspond to the long reads. `NA` indicates that the file is missing. From 8ba97d6347cf03ef0efa4fb67dec14eff4952dd7 Mon Sep 17 00:00:00 2001 From: d4straub Date: Fri, 20 Aug 2021 10:56:49 +0200 Subject: [PATCH 69/81] fix nanopolish input --- modules/local/nanopolish.nf | 4 ++-- workflows/bacass.nf | 3 ++- 2 files changed, 4 insertions(+), 3 deletions(-) diff --git a/modules/local/nanopolish.nf b/modules/local/nanopolish.nf index ee579bd6..f346d091 100644 --- a/modules/local/nanopolish.nf +++ b/modules/local/nanopolish.nf @@ -22,7 +22,7 @@ process NANOPOLISH { } input: - tuple val(meta), val(reads), file(longreads), file(assembly), file(bai), file(fast5) + tuple val(meta), val(reads), file(longreads), file(assembly), file(bai), file(bai), file(fast5) output: tuple val(meta), file('polished_genome.fa'), emit: assembly @@ -38,7 +38,7 @@ process NANOPOLISH { --consensus \ -o polished.vcf \ -r "${longreads}" \ - -b "${prefix}.bam" \ + -b "${bam}" \ -g "${assembly}" \ -t "${task.cpus}" \ --min-candidate-frequency 0.1 diff --git a/workflows/bacass.nf b/workflows/bacass.nf index f4f1cd2d..cb883653 100644 --- a/workflows/bacass.nf +++ b/workflows/bacass.nf @@ -262,9 +262,10 @@ workflow BACASS { ) ch_software_versions = ch_software_versions.mix(SAMTOOLS_INDEX.out.version.first().ifEmpty(null)) ch_for_polish //tuple val(meta), val(reads), file(longreads), file(assembly) + .join( SAMTOOLS_SORT.out.bam ) //tuple val(meta), file(bam) .join( SAMTOOLS_INDEX.out.bai ) //tuple val(meta), file(bai) .join( INPUT_CHECK.out.fast5 ) //tuple val(meta), file(fast5) - .set { ch_for_nanopolish } //tuple val(meta), val(reads), file(longreads), file(assembly), file(bai), file(fast5) + .set { ch_for_nanopolish } //tuple val(meta), val(reads), file(longreads), file(assembly), file(bam), file(bai), file(fast5) NANOPOLISH ( ch_for_nanopolish.dump(tag: 'into_nanopolish') ) From ae2a20f04920c35725537735ce9337ebcb0602a5 Mon Sep 17 00:00:00 2001 From: d4straub Date: Fri, 20 Aug 2021 10:58:26 +0200 Subject: [PATCH 70/81] fix nanopolish input --- modules/local/nanopolish.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/modules/local/nanopolish.nf b/modules/local/nanopolish.nf index f346d091..c1e6c943 100644 --- a/modules/local/nanopolish.nf +++ b/modules/local/nanopolish.nf @@ -22,7 +22,7 @@ process NANOPOLISH { } input: - tuple val(meta), val(reads), file(longreads), file(assembly), file(bai), file(bai), file(fast5) + tuple val(meta), val(reads), file(longreads), file(assembly), file(bam), file(bai), file(fast5) output: tuple val(meta), file('polished_genome.fa'), emit: assembly From 1455d9dc225bc93d9a99879e1338cc50bb51e5aa Mon Sep 17 00:00:00 2001 From: d4straub Date: Fri, 20 Aug 2021 12:28:40 +0200 Subject: [PATCH 71/81] remove unused samplesheet check and solve some TODOs --- README.md | 2 +- bin/check_samplesheet.py | 146 ----------------------------- docs/usage.md | 4 - modules/local/samplesheet_check.nf | 31 ------ subworkflows/local/input_check.nf | 2 - workflows/bacass.nf | 3 +- 6 files changed, 2 insertions(+), 186 deletions(-) delete mode 100755 bin/check_samplesheet.py delete mode 100644 modules/local/samplesheet_check.nf diff --git a/README.md b/README.md index 23860045..17d23ac9 100644 --- a/README.md +++ b/README.md @@ -21,7 +21,7 @@ The pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool to run tasks across multiple compute infrastructures in a very portable manner. It uses Docker/Singularity containers making installation trivial and results highly reproducible. The [Nextflow DSL2](https://www.nextflow.io/docs/latest/dsl2.html) implementation of this pipeline uses one container per process which makes it much easier to maintain and update software dependencies. Where possible, these processes have been submitted to and installed from [nf-core/modules](https://github.com/nf-core/modules) in order to make them available to all nf-core pipelines, and to everyone within the Nextflow community! -On release, automated continuous integration tests run the pipeline on a full-sized dataset on the AWS cloud infrastructure. This ensures that the pipeline runs on AWS, has sensible resource allocation defaults set to run on real-world datasets, and permits the persistent storage of results to benchmark between pipeline releases and other analysis sources. The results obtained from the full-sized test can be viewed on the [nf-core website](https://nf-co.re/bacass/results). + ## Pipeline summary diff --git a/bin/check_samplesheet.py b/bin/check_samplesheet.py deleted file mode 100755 index cef3e4fe..00000000 --- a/bin/check_samplesheet.py +++ /dev/null @@ -1,146 +0,0 @@ -#!/usr/bin/env python - -# TODO nf-core: Update the script to check the samplesheet -# This script is based on the example at: https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv - -import os -import sys -import errno -import argparse - - -def parse_args(args=None): - Description = "Reformat nf-core/bacass samplesheet file and check its contents." - Epilog = "Example usage: python check_samplesheet.py " - - parser = argparse.ArgumentParser(description=Description, epilog=Epilog) - parser.add_argument("FILE_IN", help="Input samplesheet file.") - parser.add_argument("FILE_OUT", help="Output file.") - return parser.parse_args(args) - - -def make_dir(path): - if len(path) > 0: - try: - os.makedirs(path) - except OSError as exception: - if exception.errno != errno.EEXIST: - raise exception - - -def print_error(error, context="Line", context_str=""): - error_str = "ERROR: Please check samplesheet -> {}".format(error) - if context != "" and context_str != "": - error_str = "ERROR: Please check samplesheet -> {}\n{}: '{}'".format( - error, context.strip(), context_str.strip() - ) - print(error_str) - sys.exit(1) - - -# TODO nf-core: Update the check_samplesheet function -def check_samplesheet(file_in, file_out): - """ - This function checks that the samplesheet follows the following structure: - - sample,fastq_1,fastq_2 - SAMPLE_PE,SAMPLE_PE_RUN1_1.fastq.gz,SAMPLE_PE_RUN1_2.fastq.gz - SAMPLE_PE,SAMPLE_PE_RUN2_1.fastq.gz,SAMPLE_PE_RUN2_2.fastq.gz - SAMPLE_SE,SAMPLE_SE_RUN1_1.fastq.gz, - - For an example see: - https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv - """ - - sample_mapping_dict = {} - with open(file_in, "r") as fin: - - ## Check header - MIN_COLS = 2 - # TODO nf-core: Update the column names for the input samplesheet - HEADER = ["sample", "fastq_1", "fastq_2"] - header = [x.strip('"') for x in fin.readline().strip().split(",")] - if header[: len(HEADER)] != HEADER: - print("ERROR: Please check samplesheet header -> {} != {}".format(",".join(header), ",".join(HEADER))) - sys.exit(1) - - ## Check sample entries - for line in fin: - lspl = [x.strip().strip('"') for x in line.strip().split(",")] - - # Check valid number of columns per row - if len(lspl) < len(HEADER): - print_error( - "Invalid number of columns (minimum = {})!".format(len(HEADER)), - "Line", - line, - ) - num_cols = len([x for x in lspl if x]) - if num_cols < MIN_COLS: - print_error( - "Invalid number of populated columns (minimum = {})!".format(MIN_COLS), - "Line", - line, - ) - - ## Check sample name entries - sample, fastq_1, fastq_2 = lspl[: len(HEADER)] - sample = sample.replace(" ", "_") - if not sample: - print_error("Sample entry has not been specified!", "Line", line) - - ## Check FastQ file extension - for fastq in [fastq_1, fastq_2]: - if fastq: - if fastq.find(" ") != -1: - print_error("FastQ file contains spaces!", "Line", line) - if not fastq.endswith(".fastq.gz") and not fastq.endswith(".fq.gz"): - print_error( - "FastQ file does not have extension '.fastq.gz' or '.fq.gz'!", - "Line", - line, - ) - - ## Auto-detect paired-end/single-end - sample_info = [] ## [single_end, fastq_1, fastq_2] - if sample and fastq_1 and fastq_2: ## Paired-end short reads - sample_info = ["0", fastq_1, fastq_2] - elif sample and fastq_1 and not fastq_2: ## Single-end short reads - sample_info = ["1", fastq_1, fastq_2] - else: - print_error("Invalid combination of columns provided!", "Line", line) - - ## Create sample mapping dictionary = { sample: [ single_end, fastq_1, fastq_2 ] } - if sample not in sample_mapping_dict: - sample_mapping_dict[sample] = [sample_info] - else: - if sample_info in sample_mapping_dict[sample]: - print_error("Samplesheet contains duplicate rows!", "Line", line) - else: - sample_mapping_dict[sample].append(sample_info) - - ## Write validated samplesheet with appropriate columns - if len(sample_mapping_dict) > 0: - out_dir = os.path.dirname(file_out) - make_dir(out_dir) - with open(file_out, "w") as fout: - fout.write(",".join(["sample", "single_end", "fastq_1", "fastq_2"]) + "\n") - for sample in sorted(sample_mapping_dict.keys()): - - ## Check that multiple runs of the same sample are of the same datatype - if not all(x[0] == sample_mapping_dict[sample][0][0] for x in sample_mapping_dict[sample]): - print_error("Multiple runs of a sample must be of the same datatype!", "Sample: {}".format(sample)) - - for idx, val in enumerate(sample_mapping_dict[sample]): - fout.write(",".join(["{}_T{}".format(sample, idx + 1)] + val) + "\n") - else: - print_error("No entries to process!", "Samplesheet: {}".format(file_in)) - - -def main(args=None): - args = parse_args(args) - check_samplesheet(args.FILE_IN, args.FILE_OUT) - - -if __name__ == "__main__": - sys.exit(main()) diff --git a/docs/usage.md b/docs/usage.md index b3407e36..a2cab431 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -6,10 +6,6 @@ ## Introduction - - -## Samplesheet input - You will need to create a samplesheet with information about the samples you would like to analyse before running the pipeline. Use this parameter to specify its location. It has to be a comma-separated file with 3 columns, and a header row as shown in the examples below. ```console diff --git a/modules/local/samplesheet_check.nf b/modules/local/samplesheet_check.nf deleted file mode 100644 index e5ba91c2..00000000 --- a/modules/local/samplesheet_check.nf +++ /dev/null @@ -1,31 +0,0 @@ -// Import generic module functions -include { saveFiles } from './functions' - -params.options = [:] - -process SAMPLESHEET_CHECK { - tag "$samplesheet" - publishDir "${params.outdir}", - mode: params.publish_dir_mode, - saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:'pipeline_info', meta:[:], publish_by_meta:[]) } - - conda (params.enable_conda ? "conda-forge::python=3.8.3" : null) - if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://depot.galaxyproject.org/singularity/python:3.8.3" - } else { - container "quay.io/biocontainers/python:3.8.3" - } - - input: - path samplesheet - - output: - path '*.csv' - - script: // This script is bundled with the pipeline, in nf-core/bacass/bin/ - """ - check_samplesheet.py \\ - $samplesheet \\ - samplesheet.valid.csv - """ -} diff --git a/subworkflows/local/input_check.nf b/subworkflows/local/input_check.nf index dfb05ecb..7fb9249c 100644 --- a/subworkflows/local/input_check.nf +++ b/subworkflows/local/input_check.nf @@ -4,8 +4,6 @@ params.options = [:] -include { SAMPLESHEET_CHECK } from '../../modules/local/samplesheet_check' addParams( options: params.options ) - workflow INPUT_CHECK { take: samplesheet // file: /path/to/samplesheet.csv diff --git a/workflows/bacass.nf b/workflows/bacass.nf index cb883653..8a8de179 100644 --- a/workflows/bacass.nf +++ b/workflows/bacass.nf @@ -9,9 +9,8 @@ def summary_params = NfcoreSchema.paramsSummaryMap(workflow, params) // Validate input parameters WorkflowBacass.initialise(params, log) -// TODO nf-core: Add all file path parameters for the pipeline to the list below // Check input path parameters to see if they exist -def checkPathParamList = [ params.input, params.multiqc_config, params.fasta ] +def checkPathParamList = [ params.input, params.multiqc_config, params.fasta, params.kraken2db, params.dfast_config ] for (param in checkPathParamList) { if (param) { file(param, checkIfExists: true) } } // Check mandatory parameters From 61df69de992e6fdc72687530f95d77babf1c7e85 Mon Sep 17 00:00:00 2001 From: d4straub Date: Mon, 23 Aug 2021 14:16:41 +0200 Subject: [PATCH 72/81] remove any connection to igenomes --- conf/igenomes.config | 432 -------------------------------------- lib/WorkflowBacass.groovy | 16 -- lib/WorkflowMain.groovy | 13 -- main.nf | 8 - nextflow.config | 14 +- nextflow_schema.json | 43 ---- workflows/bacass.nf | 2 +- 7 files changed, 2 insertions(+), 526 deletions(-) delete mode 100644 conf/igenomes.config diff --git a/conf/igenomes.config b/conf/igenomes.config deleted file mode 100644 index 855948de..00000000 --- a/conf/igenomes.config +++ /dev/null @@ -1,432 +0,0 @@ -/* -======================================================================================== - Nextflow config file for iGenomes paths -======================================================================================== - Defines reference genomes using iGenome paths. - Can be used by any config that customises the base path using: - $params.igenomes_base / --igenomes_base ----------------------------------------------------------------------------------------- -*/ - -params { - // illumina iGenomes reference file paths - genomes { - 'GRCh37' { - fasta = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Annotation/README.txt" - mito_name = "MT" - macs_gsize = "2.7e9" - blacklist = "${projectDir}/assets/blacklists/GRCh37-blacklist.bed" - } - 'GRCh38' { - fasta = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Annotation/Genes/genes.bed" - mito_name = "chrM" - macs_gsize = "2.7e9" - blacklist = "${projectDir}/assets/blacklists/hg38-blacklist.bed" - } - 'GRCm38' { - fasta = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Annotation/README.txt" - mito_name = "MT" - macs_gsize = "1.87e9" - blacklist = "${projectDir}/assets/blacklists/GRCm38-blacklist.bed" - } - 'TAIR10' { - fasta = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Annotation/README.txt" - mito_name = "Mt" - } - 'EB2' { - fasta = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Annotation/README.txt" - } - 'UMD3.1' { - fasta = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Annotation/README.txt" - mito_name = "MT" - } - 'WBcel235' { - fasta = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Annotation/Genes/genes.bed" - mito_name = "MtDNA" - macs_gsize = "9e7" - } - 'CanFam3.1' { - fasta = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Annotation/README.txt" - mito_name = "MT" - } - 'GRCz10' { - fasta = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Annotation/Genes/genes.bed" - mito_name = "MT" - } - 'BDGP6' { - fasta = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Annotation/Genes/genes.bed" - mito_name = "M" - macs_gsize = "1.2e8" - } - 'EquCab2' { - fasta = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Annotation/README.txt" - mito_name = "MT" - } - 'EB1' { - fasta = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Annotation/README.txt" - } - 'Galgal4' { - fasta = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Annotation/Genes/genes.bed" - mito_name = "MT" - } - 'Gm01' { - fasta = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Annotation/README.txt" - } - 'Mmul_1' { - fasta = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Annotation/README.txt" - mito_name = "MT" - } - 'IRGSP-1.0' { - fasta = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Annotation/Genes/genes.bed" - mito_name = "Mt" - } - 'CHIMP2.1.4' { - fasta = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Annotation/README.txt" - mito_name = "MT" - } - 'Rnor_5.0' { - fasta = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Annotation/Genes/genes.bed" - mito_name = "MT" - } - 'Rnor_6.0' { - fasta = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Annotation/Genes/genes.bed" - mito_name = "MT" - } - 'R64-1-1' { - fasta = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Annotation/Genes/genes.bed" - mito_name = "MT" - macs_gsize = "1.2e7" - } - 'EF2' { - fasta = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Annotation/README.txt" - mito_name = "MT" - macs_gsize = "1.21e7" - } - 'Sbi1' { - fasta = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Annotation/README.txt" - } - 'Sscrofa10.2' { - fasta = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Annotation/README.txt" - mito_name = "MT" - } - 'AGPv3' { - fasta = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Annotation/Genes/genes.bed" - mito_name = "Mt" - } - 'hg38' { - fasta = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Annotation/Genes/genes.bed" - mito_name = "chrM" - macs_gsize = "2.7e9" - blacklist = "${projectDir}/assets/blacklists/hg38-blacklist.bed" - } - 'hg19' { - fasta = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Annotation/README.txt" - mito_name = "chrM" - macs_gsize = "2.7e9" - blacklist = "${projectDir}/assets/blacklists/hg19-blacklist.bed" - } - 'mm10' { - fasta = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Annotation/README.txt" - mito_name = "chrM" - macs_gsize = "1.87e9" - blacklist = "${projectDir}/assets/blacklists/mm10-blacklist.bed" - } - 'bosTau8' { - fasta = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Annotation/Genes/genes.bed" - mito_name = "chrM" - } - 'ce10' { - fasta = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Annotation/README.txt" - mito_name = "chrM" - macs_gsize = "9e7" - } - 'canFam3' { - fasta = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Annotation/README.txt" - mito_name = "chrM" - } - 'danRer10' { - fasta = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Annotation/Genes/genes.bed" - mito_name = "chrM" - macs_gsize = "1.37e9" - } - 'dm6' { - fasta = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Annotation/Genes/genes.bed" - mito_name = "chrM" - macs_gsize = "1.2e8" - } - 'equCab2' { - fasta = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Annotation/README.txt" - mito_name = "chrM" - } - 'galGal4' { - fasta = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Annotation/README.txt" - mito_name = "chrM" - } - 'panTro4' { - fasta = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Annotation/README.txt" - mito_name = "chrM" - } - 'rn6' { - fasta = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Annotation/Genes/genes.bed" - mito_name = "chrM" - } - 'sacCer3' { - fasta = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/BismarkIndex/" - readme = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Annotation/README.txt" - mito_name = "chrM" - macs_gsize = "1.2e7" - } - 'susScr3' { - fasta = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Annotation/README.txt" - mito_name = "chrM" - } - } -} diff --git a/lib/WorkflowBacass.groovy b/lib/WorkflowBacass.groovy index 79d7fba0..4b99b4dc 100755 --- a/lib/WorkflowBacass.groovy +++ b/lib/WorkflowBacass.groovy @@ -8,8 +8,6 @@ class WorkflowBacass { // Check and validate parameters // public static void initialise(params, log) { - genomeExistsError(params, log) - if(("${params.assembler}" == 'canu' || "${params.assembler}" == 'miniasm') && ("${params.assembly_type}" == 'short' || "${params.assembly_type}" == 'hybrid')){ log.error "Canu and Miniasm can only be used for long read assembly and neither for Hybrid nor Shortread assembly!" System.exit(1) @@ -42,18 +40,4 @@ class WorkflowBacass { yaml_file_text += "${summary_section}" return yaml_file_text } - - // - // Exit pipeline if incorrect --genome key provided - // - private static void genomeExistsError(params, log) { - if (params.genomes && params.genome && !params.genomes.containsKey(params.genome)) { - log.error "=============================================================================\n" + - " Genome '${params.genome}' not found in any config files provided to the pipeline.\n" + - " Currently, the available genome keys are:\n" + - " ${params.genomes.keySet().join(", ")}\n" + - "===================================================================================" - System.exit(1) - } - } } diff --git a/lib/WorkflowMain.groovy b/lib/WorkflowMain.groovy index d2827006..049df694 100755 --- a/lib/WorkflowMain.groovy +++ b/lib/WorkflowMain.groovy @@ -77,17 +77,4 @@ class WorkflowMain { System.exit(1) } } - - // - // Get attribute from genome config file e.g. fasta - // - public static String getGenomeAttribute(params, attribute) { - def val = '' - if (params.genomes && params.genome && params.genomes.containsKey(params.genome)) { - if (params.genomes[ params.genome ].containsKey(attribute)) { - val = params.genomes[ params.genome ][ attribute ] - } - } - return val - } } diff --git a/main.nf b/main.nf index 09ddaab9..2ce50b0f 100644 --- a/main.nf +++ b/main.nf @@ -11,14 +11,6 @@ nextflow.enable.dsl = 2 -/* -======================================================================================== - GENOME PARAMETER VALUES -======================================================================================== -*/ - -params.fasta = WorkflowMain.getGenomeAttribute(params, 'fasta') - /* ======================================================================================== VALIDATE & PRINT PARAMETER SUMMARY diff --git a/nextflow.config b/nextflow.config index 8dc8e31b..64535ae2 100644 --- a/nextflow.config +++ b/nextflow.config @@ -35,11 +35,6 @@ params { skip_annotation = false skip_polish = false - // References - genome = null - igenomes_base = 's3://ngi-igenomes/igenomes' - igenomes_ignore = false - // MultiQC options multiqc_config = null multiqc_title = null @@ -56,7 +51,7 @@ params { help = false validate_params = true show_hidden_params = false - schema_ignore_params = 'genomes,modules' + schema_ignore_params = 'modules' enable_conda = false singularity_pull_docker_container = false @@ -90,13 +85,6 @@ try { System.err.println("WARNING: Could not load nf-core/config profiles: ${params.custom_config_base}/nfcore_custom.config") } -// Load igenomes.config if required -if (!params.igenomes_ignore) { - includeConfig 'conf/igenomes.config' -} else { - params.genomes = [:] -} - profiles { debug { process.beforeScript = 'echo $HOSTNAME' } conda { diff --git a/nextflow_schema.json b/nextflow_schema.json index 356a5d7d..216c26fd 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -157,46 +157,6 @@ } } }, - "reference_genome_options": { - "title": "Reference genome options", - "type": "object", - "fa_icon": "fas fa-dna", - "description": "Reference genome related files and options required for the workflow.", - "properties": { - "genome": { - "type": "string", - "description": "Name of iGenomes reference.", - "fa_icon": "fas fa-book", - "help_text": "If using a reference genome configured in the pipeline using iGenomes, use this parameter to give the ID for the reference. This is then used to build the full paths for all required reference genome files e.g. `--genome GRCh38`. \n\nSee the [nf-core website docs](https://nf-co.re/usage/reference_genomes) for more details.", - "hidden": true - }, - "fasta": { - "type": "string", - "format": "file-path", - "mimetype": "text/plain", - "pattern": "^\\S+\\.fn?a(sta)?(\\.gz)?$", - "description": "Path to FASTA genome file.", - "help_text": "This parameter is *mandatory* if `--genome` is not specified. If you don't have a BWA index available this will be generated for you automatically. Combine with `--save_reference` to save BWA index for future runs.", - "fa_icon": "far fa-file-code", - "hidden": true - }, - "igenomes_base": { - "type": "string", - "format": "directory-path", - "description": "Directory / URL base for iGenomes references.", - "default": "s3://ngi-igenomes/igenomes", - "fa_icon": "fas fa-cloud-download-alt", - "hidden": true - }, - "igenomes_ignore": { - "type": "boolean", - "description": "Do not load the iGenomes reference config.", - "fa_icon": "fas fa-ban", - "hidden": true, - "help_text": "Do not load `igenomes.config` when running the pipeline. You may choose this option if you observe clashes between custom parameters and those supplied in `igenomes.config`." - } - } - }, "institutional_config_options": { "title": "Institutional config options", "type": "object", @@ -410,9 +370,6 @@ { "$ref": "#/definitions/skipping_options" }, - { - "$ref": "#/definitions/reference_genome_options" - }, { "$ref": "#/definitions/institutional_config_options" }, diff --git a/workflows/bacass.nf b/workflows/bacass.nf index 8a8de179..a7eeba15 100644 --- a/workflows/bacass.nf +++ b/workflows/bacass.nf @@ -10,7 +10,7 @@ def summary_params = NfcoreSchema.paramsSummaryMap(workflow, params) WorkflowBacass.initialise(params, log) // Check input path parameters to see if they exist -def checkPathParamList = [ params.input, params.multiqc_config, params.fasta, params.kraken2db, params.dfast_config ] +def checkPathParamList = [ params.input, params.multiqc_config, params.kraken2db, params.dfast_config ] for (param in checkPathParamList) { if (param) { file(param, checkIfExists: true) } } // Check mandatory parameters From a1baa68eb36e0fa7498f40297b2fde0acfdaa846 Mon Sep 17 00:00:00 2001 From: d4straub Date: Mon, 23 Aug 2021 15:21:19 +0200 Subject: [PATCH 73/81] add full size test dataset --- .github/workflows/awsfulltest.yml | 1 - CHANGELOG.md | 2 ++ CITATIONS.md | 7 ++++++- README.md | 3 +-- conf/test_full.config | 8 ++------ 5 files changed, 11 insertions(+), 10 deletions(-) diff --git a/.github/workflows/awsfulltest.yml b/.github/workflows/awsfulltest.yml index dac0a97d..5e94f7df 100644 --- a/.github/workflows/awsfulltest.yml +++ b/.github/workflows/awsfulltest.yml @@ -15,7 +15,6 @@ jobs: steps: - name: Launch workflow via tower uses: nf-core/tower-action@master - # TODO nf-core: You can customise AWS full pipeline tests as required # Add full size test data (but still relatively small datasets for few samples) # on the `test_full.config` test runs with only one set of parameters diff --git a/CHANGELOG.md b/CHANGELOG.md index f8afad75..3f305fa9 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -12,6 +12,8 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 ### `Added` +* [#56](https://github.com/nf-core/bacass/pull/56) - Added full size test dataset, two Zetaproteobacteria sequenced with Illumina MiSeq Reagent Kit V2, PE250, 3 to 4 million read pairs. + ### `Fixed` * [#51](https://github.com/nf-core/bacass/issues/51) - Fixed Unicycler diff --git a/CITATIONS.md b/CITATIONS.md index 5e061325..dfec0ec6 100644 --- a/CITATIONS.md +++ b/CITATIONS.md @@ -50,7 +50,12 @@ > Wood, D et al., 2019. Improved metagenomic analysis with Kraken 2. Genome Biology volume 20, Article number: 257. doi: 10.1186/s13059-019-1891-0. * [MultiQC](https://www.ncbi.nlm.nih.gov/pubmed/27312411/) - > Ewels P, Magnusson M, Lundin S, Käller M. MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics. 2016 Oct 1;32(19):3047-8. doi: 10.1093/bioinformatics/btw354. Epub 2016 Jun 16. PubMed PMID: 27312411; PubMed Central PMCID: PMC5039924. + > Ewels P, Magnusson M, Lundin S, Käller M. MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics. 2016 Oct 1;32(19):3047-8. doi: 10.1093/bioinformatics/btw354. Epub 2016 Jun 16. PubMed PMID: 27312411; PubMed Central PMCID: PMC5039924 + +## Data + +* [Full-size test data](https://pubmed.ncbi.nlm.nih.gov/32561582/) + > Blackwell N, Bryce C, Straub D, Kappler A, Kleindienst S. Genomic Insights into Two Novel Fe(II)-Oxidizing Zetaproteobacteria Isolates Reveal Lifestyle Adaption to Coastal Marine Sediments. Appl Environ Microbiol. 2020 Aug 18;86(17):e01160-20. doi: 10.1128/AEM.01160-20. PMID: 32561582; PMCID: PMC7440796. ## Software packaging/containerisation tools diff --git a/README.md b/README.md index 17d23ac9..09ab5599 100644 --- a/README.md +++ b/README.md @@ -20,8 +20,7 @@ The pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool to run tasks across multiple compute infrastructures in a very portable manner. It uses Docker/Singularity containers making installation trivial and results highly reproducible. The [Nextflow DSL2](https://www.nextflow.io/docs/latest/dsl2.html) implementation of this pipeline uses one container per process which makes it much easier to maintain and update software dependencies. Where possible, these processes have been submitted to and installed from [nf-core/modules](https://github.com/nf-core/modules) in order to make them available to all nf-core pipelines, and to everyone within the Nextflow community! - - +On release, automated continuous integration tests run the pipeline on a full-sized dataset on the AWS cloud infrastructure. This ensures that the pipeline runs on AWS, has sensible resource allocation defaults set to run on real-world datasets, and permits the persistent storage of results to benchmark between pipeline releases and other analysis sources. The results obtained from the full-sized test can be viewed on the [nf-core website](https://nf-co.re/bacass/results). ## Pipeline summary diff --git a/conf/test_full.config b/conf/test_full.config index 1bac8cd3..298a4447 100644 --- a/conf/test_full.config +++ b/conf/test_full.config @@ -15,10 +15,6 @@ params { config_profile_description = 'Full test dataset to check pipeline function' // Input data for full size test - // TODO nf-core: Specify the paths to your full test data ( on nf-core/test-datasets or directly in repositories, e.g. SRA) - // TODO nf-core: Give any required params for the test so that command line flags are not needed - input = 'https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_full_illumina_amplicon.csv' - - // Genome references - genome = 'R64-1-1' + input = 'https://raw.githubusercontent.com/nf-core/test-datasets/bacass/bacass_full.csv' + kraken2db = 'https://genome-idx.s3.amazonaws.com/kraken/k2_standard_8gb_20210517.tar.gz' } From 97a1ea85e80ff9087832f485e301af728561b369 Mon Sep 17 00:00:00 2001 From: d4straub Date: Mon, 23 Aug 2021 15:33:51 +0200 Subject: [PATCH 74/81] removed unused parameter from test_long --- conf/test_long.config | 1 - 1 file changed, 1 deletion(-) diff --git a/conf/test_long.config b/conf/test_long.config index d87d3765..be225894 100644 --- a/conf/test_long.config +++ b/conf/test_long.config @@ -24,7 +24,6 @@ params { // some extra args to speed tests up prokka_args = " --fast" - canu_args = 'stopOnLowCoverage=0 minInputCoverage=0 corMinCoverage=0 minOverlapLength=0 minReadLength=0' assembly_type = 'long' skip_polish = true skip_kraken2 = true From 002fcd090d126083ae5dd4e8e6e6c79b3604a161 Mon Sep 17 00:00:00 2001 From: d4straub Date: Mon, 23 Aug 2021 16:45:29 +0200 Subject: [PATCH 75/81] ignore igenomes_base to avoid frequent warning with institutional profiles --- nextflow.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nextflow.config b/nextflow.config index 64535ae2..6e8eea7f 100644 --- a/nextflow.config +++ b/nextflow.config @@ -51,7 +51,7 @@ params { help = false validate_params = true show_hidden_params = false - schema_ignore_params = 'modules' + schema_ignore_params = 'modules,igenomes_base' enable_conda = false singularity_pull_docker_container = false From 9948e4fbc0e535014720582687d7a1066039d1eb Mon Sep 17 00:00:00 2001 From: d4straub Date: Thu, 26 Aug 2021 09:28:05 +0200 Subject: [PATCH 76/81] update README --- README.md | 6 +----- 1 file changed, 1 insertion(+), 5 deletions(-) diff --git a/README.md b/README.md index 09ab5599..ac56aa49 100644 --- a/README.md +++ b/README.md @@ -77,11 +77,7 @@ The nf-core/bacass pipeline comes with documentation about the pipeline [usage]( ## Credits -nf-core/bacass was originally written by Andreas Wilm, Alexander Peltzer. - -We thank the following people for their extensive assistance in the development of this pipeline: - -* Daniel Straub ([@d4straub](https://github.com/d4straub)) +nf-core/bacass was initiated by Andreas Wilm, written by Alex Peltzer (DSL1), rewritten by [Daniel Straub](https://github.com/d4straub) (DSL2). ## Contributions and Support From f4117ed3b9c2be6bc70acaf02b272a61b69b3e5f Mon Sep 17 00:00:00 2001 From: d4straub Date: Thu, 26 Aug 2021 09:34:00 +0200 Subject: [PATCH 77/81] bump version --- CHANGELOG.md | 2 +- nextflow.config | 4 ++-- 2 files changed, 3 insertions(+), 3 deletions(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 3f305fa9..d9b44c37 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -3,7 +3,7 @@ The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html). -## v2.0.0dev - [date] +## v2.0.0 nf-core/bacass: "Navy Steel Swordfish" 2021/08/27 ### `Changed` diff --git a/nextflow.config b/nextflow.config index 6e8eea7f..cc46a1c2 100644 --- a/nextflow.config +++ b/nextflow.config @@ -175,7 +175,7 @@ manifest { description = 'Simple bacterial assembly and annotation' mainScript = 'main.nf' nextflowVersion = '!>=21.04.0' - version = '2.0.0dev' + version = '2.0.0' } // Function to ensure that resource requirements don't go beyond @@ -209,4 +209,4 @@ def check_max(obj, type) { return obj } } -} +} \ No newline at end of file From 409954c13a45e768f9e18de3be4bf1dd5097f895 Mon Sep 17 00:00:00 2001 From: d4straub Date: Thu, 26 Aug 2021 09:38:10 +0200 Subject: [PATCH 78/81] fix linting --- nextflow.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nextflow.config b/nextflow.config index cc46a1c2..3bce38bd 100644 --- a/nextflow.config +++ b/nextflow.config @@ -209,4 +209,4 @@ def check_max(obj, type) { return obj } } -} \ No newline at end of file +} From 0fa53929c0f89a28efebc4962fb4b3599cbeb838 Mon Sep 17 00:00:00 2001 From: Daniel Straub <42973691+d4straub@users.noreply.github.com> Date: Thu, 26 Aug 2021 09:47:48 +0200 Subject: [PATCH 79/81] Update README.md Co-authored-by: Alexander Peltzer --- README.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/README.md b/README.md index ac56aa49..ac07cbd7 100644 --- a/README.md +++ b/README.md @@ -77,7 +77,7 @@ The nf-core/bacass pipeline comes with documentation about the pipeline [usage]( ## Credits -nf-core/bacass was initiated by Andreas Wilm, written by Alex Peltzer (DSL1), rewritten by [Daniel Straub](https://github.com/d4straub) (DSL2). +nf-core/bacass was initiated by [Andreas Wilm](https://github.com/andreas-wilm), originally written by [Alex Peltzer](https://github.com/apeltzer) (DSLv1) and rewritten by [Daniel Straub](https://github.com/d4straub) (DSLv2). ## Contributions and Support From ef609bd03dd47326d12c644f5eb0a1f5320e55a8 Mon Sep 17 00:00:00 2001 From: d4straub Date: Thu, 26 Aug 2021 10:26:03 +0200 Subject: [PATCH 80/81] fix QUAST input for AWS --- workflows/bacass.nf | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/workflows/bacass.nf b/workflows/bacass.nf index a7eeba15..c776ba5f 100644 --- a/workflows/bacass.nf +++ b/workflows/bacass.nf @@ -317,8 +317,8 @@ workflow BACASS { .set { ch_to_quast } QUAST ( ch_to_quast, - file('fasta'), - file('gff'), + [], + [], false, false ) From 415e2eac319b3555fb8478689fa1ea4e12e35462 Mon Sep 17 00:00:00 2001 From: d4straub Date: Fri, 27 Aug 2021 10:58:00 +0200 Subject: [PATCH 81/81] resolve reviewer commets --- README.md | 2 +- workflows/bacass.nf | 2 +- 2 files changed, 2 insertions(+), 2 deletions(-) diff --git a/README.md b/README.md index ac07cbd7..3865ec47 100644 --- a/README.md +++ b/README.md @@ -77,7 +77,7 @@ The nf-core/bacass pipeline comes with documentation about the pipeline [usage]( ## Credits -nf-core/bacass was initiated by [Andreas Wilm](https://github.com/andreas-wilm), originally written by [Alex Peltzer](https://github.com/apeltzer) (DSLv1) and rewritten by [Daniel Straub](https://github.com/d4straub) (DSLv2). +nf-core/bacass was initiated by [Andreas Wilm](https://github.com/andreas-wilm), originally written by [Alex Peltzer](https://github.com/apeltzer) (DSL1) and rewritten by [Daniel Straub](https://github.com/d4straub) (DSL2). ## Contributions and Support diff --git a/workflows/bacass.nf b/workflows/bacass.nf index c776ba5f..1a9aaaf7 100644 --- a/workflows/bacass.nf +++ b/workflows/bacass.nf @@ -127,7 +127,7 @@ workflow BACASS { ch_software_versions = ch_software_versions.mix(FASTQC.out.version.first().ifEmpty(null)) // - // MODULE: Skewer, trim and combine short read read-pairs per sample. Similar to nf-core vipr + // MODULE: Skewer, trim and combine short read read-pairs per sample. // SKEWER ( INPUT_CHECK.out.shortreads.dump(tag: 'shortreads')
\n" + for (param in group_params.keySet()) { + summary_section += "
$param
${group_params.get(param) ?: 'N/A'}
\n" + } + summary_section += "

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