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Support not pooling reads for HOMER #153

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edmundmiller opened this issue Aug 3, 2024 · 0 comments
Open

Support not pooling reads for HOMER #153

edmundmiller opened this issue Aug 3, 2024 · 0 comments
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enhancement New feature or request

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@edmundmiller
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Description of feature

Pool the reads from both experiments into a single Tag Directory using makeTagDirectory (more details here). In the case of technical replicates (i.e. runs from the same sequencing library), it is advisable to always "pool" the data. If they are biological replicates, it is often a good idea to keep them separate for and take advantage of their variability to refine your analysis. For some types of analysis, such as transcript identification, it is a good idea to create a single META-experiment that contains all of the GRO-Seq reads for a given cell type. This will provide increased power for identifying transcripts de novo.

http://homer.ucsd.edu/homer/ngs/groseq/groseq.html

Currently, supporting technical reads getting pooled, and grouping via META-experiment.

I think either way, it requires some really careful samplesheet curation, and there aren't examples of what those would be exactly.

@edmundmiller edmundmiller added the enhancement New feature or request label Aug 3, 2024
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