diff --git a/tests/config/samplesheets/no_underscore.csv b/tests/config/samplesheets/no_underscore.csv
new file mode 100644
index 00000000..c23eb02b
--- /dev/null
+++ b/tests/config/samplesheets/no_underscore.csv
@@ -0,0 +1,4 @@
+sample,fastq_1,fastq_2
+DUTTKE,https://raw.githubusercontent.com/nf-core/test-datasets/nascent/testdata/SRX882903_T1.fastq.gz,
+EXP,https://raw.githubusercontent.com/nf-core/test-datasets/nascent/testdata/SRX882903_T2.fastq.gz,
+DUTTKE,https://raw.githubusercontent.com/nf-core/test-datasets/nascent/testdata/SRX882904_T1.fastq.gz,
diff --git a/workflows/tests/bugs/no_underscore.nf.test b/workflows/tests/bugs/no_underscore.nf.test
new file mode 100644
index 00000000..3592cd5a
--- /dev/null
+++ b/workflows/tests/bugs/no_underscore.nf.test
@@ -0,0 +1,23 @@
+nextflow_pipeline {
+
+    name "Samplesheet with no underscores"
+    script "../../../main.nf"
+    tag "bug"
+
+    // https://github.com/nf-core/nascent/issues/158
+    test("Should work with samples that don't have an underscore") {
+        when {
+            params {
+                outdir = "$outputDir"
+                input = "${projectDir}/tests/config/samplesheets/no_underscore.csv"
+            }
+        }
+
+        then {
+            assert workflow.success
+            assert snapshot(
+                workflow.trace.tasks().size(),
+            ).match()
+        }
+    }
+}
diff --git a/workflows/tests/bugs/no_underscore.nf.test.snap b/workflows/tests/bugs/no_underscore.nf.test.snap
new file mode 100644
index 00000000..97561c22
--- /dev/null
+++ b/workflows/tests/bugs/no_underscore.nf.test.snap
@@ -0,0 +1,12 @@
+{
+    "Should work with samples that don't have an underscore": {
+        "content": [
+            58
+        ],
+        "meta": {
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
+        },
+        "timestamp": "2024-08-16T22:12:17.34063593"
+    }
+}
\ No newline at end of file