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2017 Metagenomics workshop at UC Santa Cruz

Instructors: Harriet Alexander, Phillip Brooks, and C. Titus Brown

TAs: Luiz Irber, Shannon Joslin, Taylor Reiter

These are the online materials for a metagenomics workshop hosted by Marilou Sison-Mangus at UC Santa Cruz on April 22nd and April 23rd, 2017.

Etherpad

Day 1: Noon to 5pm

Logging into the cloud (XSEDE Jetstream)

Evaluating your short-read data set quality

Assembling your short read data set with MEGAHIT

Day 2: Morning (9am - noon)

Software install for day 2

Binning genomes out of your metagenome

Quickly searching and comparing your samples with sourmash

Day 2: Afternoon (1:15pm - 5pm)

Annotating your short-read data set with Prokka

Quantifying abundance across samples with Salmon

A brief discussion of workflows & repeatability

What next?

Resources:

SEQ Answers

Biostars

Data Carpentry

DIB Summer Institute

Undone

Circos for viz

What next? Workflows; CAzy, KEGG; etc.

Technical information

The github repository for this workshop is public at https://github.com/ngs-docs/2017-ucsc-metagenomics