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I'm running Ariba with the ResFinder database, and I've noticed something in the reports for individual samples. For certain genes like tetQ, which has a reference length of 1926, some contigs only cover around 500 bases, while others in the same sample cover around 1300 bases.
However, when I use a filtering script to remove entries with less than 80% coverage and identity, it removes both contigs I mentioned earlier.
My question is: should I combine the 1300 and 500 base pairs to count toward tetQ resistance, or does this mean the sample lacks sufficient coverage to include tetQ resistance?
The text was updated successfully, but these errors were encountered:
Hi,
I'm running Ariba with the ResFinder database, and I've noticed something in the reports for individual samples. For certain genes like tetQ, which has a reference length of 1926, some contigs only cover around 500 bases, while others in the same sample cover around 1300 bases.
However, when I use a filtering script to remove entries with less than 80% coverage and identity, it removes both contigs I mentioned earlier.
My question is: should I combine the 1300 and 500 base pairs to count toward tetQ resistance, or does this mean the sample lacks sufficient coverage to include tetQ resistance?
The text was updated successfully, but these errors were encountered: