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Resfinder -- interpretation #343

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ZainBioBytes opened this issue Oct 15, 2024 · 0 comments
Open

Resfinder -- interpretation #343

ZainBioBytes opened this issue Oct 15, 2024 · 0 comments

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@ZainBioBytes
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Hi,

I'm running Ariba with the ResFinder database, and I've noticed something in the reports for individual samples. For certain genes like tetQ, which has a reference length of 1926, some contigs only cover around 500 bases, while others in the same sample cover around 1300 bases.

However, when I use a filtering script to remove entries with less than 80% coverage and identity, it removes both contigs I mentioned earlier.

My question is: should I combine the 1300 and 500 base pairs to count toward tetQ resistance, or does this mean the sample lacks sufficient coverage to include tetQ resistance?

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