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Please see the specification of how matrices are generated in the 'Output' section and how MBR results are filtered (with 'Lib.' q-values) in the 'MBR' section of the docs https://github.com/vdemichev/DiaNN. |
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So far, I thought that the pg.matrix file is equivalent to the main report tsv filtered for a Global.PG.Q.Value < 1%.
However, in one example it cought my attention that the pg.matrix file has 25% more protein group entries than the main report tsv after filtering for a Global.PG.Q.Value < 1%.
I then checked the protein groups which were only contained in the pg.matrix table. These all had a Global.PG.Q.Value > 1%:
Q1: How is the pg.matrix table generated from the main report tsv?
Q2: I thought one should filter the main report tsv for Global.PG.Q.Value < 1% (unless it's a re-search using a DIA-NN library) to get quantitative data to use for biological analyses... Should one ?
Q3: If Q2 is true, people shouldn't use the pg.matrix table, should they?
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