No proteins or peptides assigned

[Bidoof11]

Hi Vadim.

I analyzed the DIA data acquired by Q-Exactive using DIA-NN, but the output report did not assign any proteins or peptides.
What could be the cause of this?
The measured samples were BSA and trypsin digested samples.
I have attached the report.
Thank you in advance.
report.log.txt

Best,
Junichi Abe

[vdemichev]

Hi Junichi,

No proteins passed the 1% FDR threshold, I guess just not much detectable in that sample. Maybe try selecting the contaminants option when both generating the library & analysing, should help. Please also generate the library in a separate pipeline step.

Best,
Vadim