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vg pack error #4384

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JONGHOLEE-myrrh opened this issue Aug 27, 2024 · 0 comments
Open

vg pack error #4384

JONGHOLEE-myrrh opened this issue Aug 27, 2024 · 0 comments

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@JONGHOLEE-myrrh
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JONGHOLEE-myrrh commented Aug 27, 2024

1. What were you trying to do?
Run "vg pack" from the gam file to make pack and vcf file.

2. What did you want to happen?
I want to generate the vcf file from the gam file.

3. What actually happened?
Error occurred. I attached the commands and errors here.

(base) [root@localhost 240821]# vg pack -t 64 -x ./00_reference/pangenome_oneline.giraffe.xg -g 02_alignment_graph/Frute1_oneline.gam -Q 5 -o 03_calling_vcf/Frute1.aln.pack
vg: src/packer.cpp:883: size_t vg::Packer::average_node_quality(size_t) const: Assertion `total_node_quality(i) == 0' failed.
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Crash report for vg v1.52.0 "Bozen"
Stack trace (most recent call last) in thread 117519:
#10   Object "", at 0xffffffffffffffff, in
#9    Object "/data/user/Public/JONG/tools/vg_1.52.0/vg", at 0x214dd33, in __clone
#8    Object "/data/user/Public/JONG/tools/vg_1.52.0/vg", at 0x20a73da, in start_thread
#7    Object "/data/user/Public/JONG/tools/vg_1.52.0/vg", at 0x2049cbd, in gomp_thread_start
#6    Object "/data/user/Public/JONG/tools/vg_1.52.0/vg", at 0x1268670, in vg::Packer::make_compact() [clone ._omp_fn.6]
#5    Object "/data/user/Public/JONG/tools/vg_1.52.0/vg", at 0x1267f7f, in vg::Packer::average_node_quality(unsigned long) const
#4    Object "/data/user/Public/JONG/tools/vg_1.52.0/vg", at 0x2075be5, in __assert_fail
#3    Object "/data/user/Public/JONG/tools/vg_1.52.0/vg", at 0x5e5833, in __assert_fail_base.cold
#2    Object "/data/user/Public/JONG/tools/vg_1.52.0/vg", at 0x5e590b, in abort
#1    Object "/data/user/Public/JONG/tools/vg_1.52.0/vg", at 0x207c1f5, in raise
#0    Object "/data/user/Public/JONG/tools/vg_1.52.0/vg", at 0x20a8bfc, in __pthread_kill
ERROR: Signal 6 occurred. VG has crashed. Visit https://github.com/vgteam/vg/issues/new/choose to report a bug.
Please include this entire error log in your bug report!
��������������������
(base) [root@localhost 240821]# vg pack -t 64 -x ./00_reference/pangenome_oneline.giraffe.gbz -g 02_alignment_graph/Frute1_oneline.gam -Q 5 -o 03_calling_vcf/Frute1.aln.pack
vg: src/packer.cpp:883: size_t vg::Packer::average_node_quality(size_t) const: Assertion `total_node_quality(i) == 0' failed.
��������������������
Crash report for vg v1.52.0 "Bozen"
Stack trace (most recent call last) in thread 119041:
#10   Object "", at 0xffffffffffffffff, in
#9    Object "/data/user/Public/JONG/tools/vg_1.52.0/vg", at 0x214dd33, in __clone
#8    Object "/data/user/Public/JONG/tools/vg_1.52.0/vg", at 0x20a73da, in start_thread
#7    Object "/data/user/Public/JONG/tools/vg_1.52.0/vg", at 0x2049cbd, in gomp_thread_start
#6    Object "/data/user/Public/JONG/tools/vg_1.52.0/vg", at 0x1268670, in vg::Packer::make_compact() [clone ._omp_fn.6]
#5    Object "/data/user/Public/JONG/tools/vg_1.52.0/vg", at 0x1267f7f, in vg::Packer::average_node_quality(unsigned long) const
#4    Object "/data/user/Public/JONG/tools/vg_1.52.0/vg", at 0x2075be5, in __assert_fail
#3    Object "/data/user/Public/JONG/tools/vg_1.52.0/vg", at 0x5e5833, in __assert_fail_base.cold
#2    Object "/data/user/Public/JONG/tools/vg_1.52.0/vg", at 0x5e590b, in abort
#1    Object "/data/user/Public/JONG/tools/vg_1.52.0/vg", at 0x207c1f5, in raise
#0    Object "/data/user/Public/JONG/tools/vg_1.52.0/vg", at 0x20a8bfc, in __pthread_kill
ERROR: Signal 6 occurred. VG has crashed. Visit https://github.com/vgteam/vg/issues/new/choose to report a bug.
Please include this entire error log in your bug report!

4. If you got a line like Stack trace path: /somewhere/on/your/computer/stacktrace.txt, please copy-paste the contents of that file here:
N/A

Place stacktrace here.

5. What data and command can the vg dev team use to make the problem happen?

6. What does running vg version say?

vg version v1.52.0 "Bozen"
Compiled with g++ (Ubuntu 11.4.0-1ubuntu1~22.04) 11.4.0 on Linux
Linked against libstd++ 20230528
Built by jeizenga@emerald
@vgteam vgteam deleted a comment Aug 27, 2024
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@JONGHOLEE-myrrh and others