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[singlecelltoolkit] [SUGGESTION] Add script to calculate saturation curve of 10x samples #332

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dweemx opened this issue Apr 1, 2021 · 2 comments
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@dweemx
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dweemx commented Apr 1, 2021

Is your feature request related to a problem? Please describe.
Automate the generation of saturation curve plots for multiple samples

Describe the solution you'd like
Add script to calculate saturation curve for 10x samples. Script is available at https://github.com/aertslab/single_cell_toolkit

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@dweemx dweemx added the enhancement New feature or request label Apr 1, 2021
@dweemx dweemx self-assigned this Apr 1, 2021
cflerin added a commit that referenced this issue Apr 1, 2021
- Use zlib-ng base image (#325)
- Include saturation script (#332), and python 3.7 install
@cflerin
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cflerin commented Jul 19, 2021

There will be a bit of a problem with integrating this script into VSN. Currently in getDataChannel we take the cellranger outs/ directory as the input for 10x-type data. But this script reads files from a directory one level up from this, and this path is not ingested into the pipeline as far as I can tell. Changing the tenx input data type is going to be a big change with the potential to break a lot of downstream processes.

@KrisDavie
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We could add a new emit to cellranger processes which is for the intermediate files (or even specifically the required files), and we can do the same with getDataChannel, we can add a new type for tenx_intermediate or something similar, thoughts?

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