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Snakemake workflow: rna-seq-star-deseq2

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This workflow performs a differential expression analysis with STAR and Deseq2. It is currently under development. No stable release is available yet.

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Usage

Step 1: Install workflow

If you simply want to use this workflow, download and extract the latest release. If you intend to modify and further develop this workflow, fork this reposity. Please consider providing any generally applicable modifications via a pull request.

In any case, if you use this workflow in a paper, don't forget to give credits to the authors by citing the URL of this repository and, once available, its DOI.

Step 2: Configure workflow

Configure the workflow according to your needs via editing the file config.yaml and the sample sheet samples.tsv.

Step 3: Execute workflow

Test your configuration by performing a dry-run via

snakemake -n

Execute the workflow locally via

snakemake --cores $N

using $N cores or run it in a cluster environment via

snakemake --cluster qsub --jobs 100

or

snakemake --drmaa --jobs 100

See the Snakemake documentation for further details.

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RNA-seq workflow using STAR and DESeq2

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  • Python 71.0%
  • R 29.0%