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id: "ebi-metagenomics/emg-viral-pipeline-methods-description" | ||
description: "Suggested text and references to use when describing pipeline usage within the methods section of a publication." | ||
section_name: "ebi-metagenomics/emg-viral-pipeline Methods Description" | ||
section_href: "https://github.com/EBI-Metagenomics/emg-viral-pipeline" | ||
plot_type: "html" | ||
data: | | ||
<h4>Methods</h4> | ||
<p>Data was processed using ebi-metagenomics/genomes-generation v${workflow.manifest.version} (${doi_text}; <a href="https://doi.org/10.1093/nargab/lqac007">Krakau <em>et al.</em>, 2022</a>) of the nf-core collection of workflows (<a href="https://doi.org/10.1038/s41587-020-0439-x">Ewels <em>et al.</em>, 2020</a>), utilising reproducible software environments from the Bioconda (<a href="https://doi.org/10.1038/s41592-018-0046-7">Grüning <em>et al.</em>, 2018</a>) and Biocontainers (<a href="https://doi.org/10.1093/bioinformatics/btx192">da Veiga Leprevost <em>et al.</em>, 2017</a>) projects.</p> | ||
<p>The pipeline was executed with Nextflow v${workflow.nextflow.version} (<a href="https://doi.org/10.1038/nbt.3820">Di Tommaso <em>et al.</em>, 2017</a>) with the following command:</p> | ||
<pre><code>${workflow.commandLine}</code></pre> | ||
<p>${tool_citations}</p> | ||
<h4>References</h4> | ||
<ul> | ||
<li> | ||
Informative Regions In Viral Genomes | ||
<i>Viruses (2021)</i> | ||
doi: <a href="https://doi.org/10.3390/v13061164">10.3390/v13061164</a> | ||
Moreno-Gallego, Jaime Leonardo, and Alejandro Reyes | ||
</li> | ||
<li> | ||
VIRify: an integrated detection, annotation and taxonomic classification pipeline using virus-specific protein profile hidden Markov models | ||
<i>bioRxiv</i> | ||
doi: <a href="https://doi.org/10.1101/2022.08.22.504484">10.1101/2022.08.22.504484</a> | ||
Rangel-Pineros, Guillermo, et al. | ||
</li> | ||
${tool_bibliography} | ||
</ul> | ||
<div class="alert alert-info"> | ||
<h5>Notes:</h5> | ||
<ul> | ||
${nodoi_text} | ||
<li>The command above does not include parameters contained in any configs or profiles that may have been used. Ensure the config file is also uploaded with your publication!</li> | ||
<li>You should also cite all software used within this run. Check the "Software Versions" of this report to get version information.</li> | ||
</ul> | ||
</div> |
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report_comment: > | ||
This report has been generated by the <a href="https://github.com/ebi-metagenomics/emg-viral-pipeline/" target="_blank">ebi-metagenomics/emg-viral-pipeline</a> pipeline. | ||
report_section_order: | ||
"ebi-metagenomics/emg-viral-pipeline-methods-description": | ||
order: -1000 | ||
software_versions: | ||
order: -1001 | ||
"ebi-metagenomics/emg-viral-pipeline-summary": | ||
order: -1002 | ||
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export_plots: true | ||
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data_format: "yaml" | ||
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run_modules: | ||
- fastqc | ||
- fastp | ||
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## Module order | ||
module_order: | ||
- fastqc | ||
- fastp | ||
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## File name cleaning | ||
extra_fn_clean_exts: | ||
- "_fastp" | ||
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## Prettification | ||
custom_logo: "mgnify_logo.png" | ||
custom_logo_url: https://github.com/ebi-metagenomics/emg-viral-pipeline/ | ||
custom_logo_title: "ebi-metagenomics/emg-viral-pipeline" | ||
|
||
## General Stats customisation | ||
table_columns_visible: | ||
"fastp": | ||
pct_duplication: False | ||
after_filtering_q30_rate: False | ||
after_filtering_q30_bases: False | ||
filtering_result_passed_filter_reads: 3300 | ||
after_filtering_gc_content: False | ||
pct_surviving: True | ||
pct_adapter: True | ||
|
||
table_columns_placement: | ||
"fastp": | ||
pct_duplication: 3000 | ||
after_filtering_q30_rate: 3100 | ||
after_filtering_q30_bases: 3200 | ||
filtering_result_passed_filter_reads: 3300 | ||
after_filtering_gc_content: 3400 | ||
pct_surviving: 3500 | ||
pct_adapter: 3600 | ||
|
||
custom_table_header_config: | ||
general_stats_table: | ||
"Total length": | ||
hidden: True | ||
N50: | ||
hidden: True |
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process { | ||
withLabel: annotation { conda = "$baseDir/envs/python3.yaml" } | ||
withLabel: assign { conda = "$baseDir/envs/python3.yaml" } | ||
withLabel: balloon { conda = "$baseDir/envs/balloon.yaml" } | ||
withLabel: basics { conda = "$baseDir/envs/python3.yaml" } | ||
withLabel: blast { conda = "$baseDir/envs/blast.yaml" } | ||
withLabel: fastp { conda = "$baseDir/envs/fastp.yaml" } | ||
withLabel: fastqc { conda = "$baseDir/envs/fastqc.yaml" } | ||
withLabel: hmmscan { conda = "$baseDir/envs/hmmer.yaml" } | ||
withLabel: kaiju { conda = "$baseDir/envs/kaiju.yaml" } | ||
withLabel: krona { conda = "$baseDir/envs/krona.yaml" } | ||
withLabel: plot_contig_map { conda = "$baseDir/envs/r.yaml" } | ||
withLabel: multiqc { conda = "$baseDir/envs/multiqc.yaml" } | ||
withLabel: parse { conda = "$baseDir/envs/python3.yaml" } | ||
withLabel: prodigal { conda = "$baseDir/envs/prodigal.yaml" } | ||
withLabel: phanotate { conda = "$baseDir/envs/phanotate.yaml" } | ||
withLabel: python3 { conda = "$baseDir/envs/python3.yaml" } | ||
withLabel: ratio_evalue { conda = "$baseDir/envs/python3.yaml" } | ||
withLabel: ruby { conda = "$baseDir/envs/ruby.yaml" } | ||
withLabel: spades { conda = "$baseDir/envs/spades.yaml" } | ||
withLabel: virsorter { conda = "$baseDir/envs/virsorter.yaml" } | ||
withLabel: virfinder { conda = "$baseDir/envs/virfinder.yaml" } | ||
withLabel: checkV { conda = "$baseDir/envs/checkv.yaml" } | ||
withNAME: ANNOTATION { conda = "$baseDir/envs/python3.yaml" } | ||
withNAME: ASSIGN { conda = "$baseDir/envs/python3.yaml" } | ||
withNAME: BALLOON { conda = "$baseDir/envs/balloon.yaml" } | ||
withNAME: basics { conda = "$baseDir/envs/python3.yaml" } | ||
withNAME: BLAST { conda = "$baseDir/envs/blast.yaml" } | ||
withNAME: HMMSCAN { conda = "$baseDir/envs/hmmer.yaml" } | ||
withNAME: KAIJU { conda = "$baseDir/envs/kaiju.yaml" } | ||
withNAME: KRONA { conda = "$baseDir/envs/krona.yaml" } | ||
withNAME: PLOT_CONTIG_MAP { conda = "$baseDir/envs/r.yaml" } | ||
withNAME: PARSE { conda = "$baseDir/envs/python3.yaml" } | ||
withNAME: PRODIGAL { conda = "$baseDir/envs/prodigal.yaml" } | ||
withNAME: PHANOTATE { conda = "$baseDir/envs/phanotate.yaml" } | ||
withNAME: python3 { conda = "$baseDir/envs/python3.yaml" } | ||
withNAME: RATIO_EVALUE { conda = "$baseDir/envs/python3.yaml" } | ||
withNAME: ruby { conda = "$baseDir/envs/ruby.yaml" } | ||
withNAME: VIRSORTER { conda = "$baseDir/envs/virsorter.yaml" } | ||
withNAME: VIRFINDER { conda = "$baseDir/envs/virfinder.yaml" } | ||
withNAME: CHECKV { conda = "$baseDir/envs/checkv.yaml" } | ||
} |
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