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KateSakharova committed Oct 7, 2024
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35 changes: 35 additions & 0 deletions assets/methods_description_template.yml
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id: "ebi-metagenomics/emg-viral-pipeline-methods-description"
description: "Suggested text and references to use when describing pipeline usage within the methods section of a publication."
section_name: "ebi-metagenomics/emg-viral-pipeline Methods Description"
section_href: "https://github.com/EBI-Metagenomics/emg-viral-pipeline"
plot_type: "html"
data: |
<h4>Methods</h4>
<p>Data was processed using ebi-metagenomics/genomes-generation v${workflow.manifest.version} (${doi_text}; <a href="https://doi.org/10.1093/nargab/lqac007">Krakau <em>et al.</em>, 2022</a>) of the nf-core collection of workflows (<a href="https://doi.org/10.1038/s41587-020-0439-x">Ewels <em>et al.</em>, 2020</a>), utilising reproducible software environments from the Bioconda (<a href="https://doi.org/10.1038/s41592-018-0046-7">Grüning <em>et al.</em>, 2018</a>) and Biocontainers (<a href="https://doi.org/10.1093/bioinformatics/btx192">da Veiga Leprevost <em>et al.</em>, 2017</a>) projects.</p>
<p>The pipeline was executed with Nextflow v${workflow.nextflow.version} (<a href="https://doi.org/10.1038/nbt.3820">Di Tommaso <em>et al.</em>, 2017</a>) with the following command:</p>
<pre><code>${workflow.commandLine}</code></pre>
<p>${tool_citations}</p>
<h4>References</h4>
<ul>
<li>
Informative Regions In Viral Genomes
<i>Viruses (2021)</i>
doi: <a href="https://doi.org/10.3390/v13061164">10.3390/v13061164</a>
Moreno-Gallego, Jaime Leonardo, and Alejandro Reyes
</li>
<li>
VIRify: an integrated detection, annotation and taxonomic classification pipeline using virus-specific protein profile hidden Markov models
<i>bioRxiv</i>
doi: <a href="https://doi.org/10.1101/2022.08.22.504484">10.1101/2022.08.22.504484</a>
Rangel-Pineros, Guillermo, et al.
</li>
${tool_bibliography}
</ul>
<div class="alert alert-info">
<h5>Notes:</h5>
<ul>
${nodoi_text}
<li>The command above does not include parameters contained in any configs or profiles that may have been used. Ensure the config file is also uploaded with your publication!</li>
<li>You should also cite all software used within this run. Check the "Software Versions" of this report to get version information.</li>
</ul>
</div>
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61 changes: 61 additions & 0 deletions assets/multiqc_config.yml
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report_comment: >
This report has been generated by the <a href="https://github.com/ebi-metagenomics/emg-viral-pipeline/" target="_blank">ebi-metagenomics/emg-viral-pipeline</a> pipeline.
report_section_order:
"ebi-metagenomics/emg-viral-pipeline-methods-description":
order: -1000
software_versions:
order: -1001
"ebi-metagenomics/emg-viral-pipeline-summary":
order: -1002

export_plots: true

data_format: "yaml"

run_modules:
- fastqc
- fastp

## Module order
module_order:
- fastqc
- fastp

## File name cleaning
extra_fn_clean_exts:
- "_fastp"

## Prettification
custom_logo: "mgnify_logo.png"
custom_logo_url: https://github.com/ebi-metagenomics/emg-viral-pipeline/
custom_logo_title: "ebi-metagenomics/emg-viral-pipeline"

## General Stats customisation
table_columns_visible:
"fastp":
pct_duplication: False
after_filtering_q30_rate: False
after_filtering_q30_bases: False
filtering_result_passed_filter_reads: 3300
after_filtering_gc_content: False
pct_surviving: True
pct_adapter: True

table_columns_placement:
"fastp":
pct_duplication: 3000
after_filtering_q30_rate: 3100
after_filtering_q30_bases: 3200
filtering_result_passed_filter_reads: 3300
after_filtering_gc_content: 3400
pct_surviving: 3500
pct_adapter: 3600

custom_table_header_config:
general_stats_table:
"Total length":
hidden: True
N50:
hidden: True
40 changes: 18 additions & 22 deletions configs/conda.config
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process {
withLabel: annotation { conda = "$baseDir/envs/python3.yaml" }
withLabel: assign { conda = "$baseDir/envs/python3.yaml" }
withLabel: balloon { conda = "$baseDir/envs/balloon.yaml" }
withLabel: basics { conda = "$baseDir/envs/python3.yaml" }
withLabel: blast { conda = "$baseDir/envs/blast.yaml" }
withLabel: fastp { conda = "$baseDir/envs/fastp.yaml" }
withLabel: fastqc { conda = "$baseDir/envs/fastqc.yaml" }
withLabel: hmmscan { conda = "$baseDir/envs/hmmer.yaml" }
withLabel: kaiju { conda = "$baseDir/envs/kaiju.yaml" }
withLabel: krona { conda = "$baseDir/envs/krona.yaml" }
withLabel: plot_contig_map { conda = "$baseDir/envs/r.yaml" }
withLabel: multiqc { conda = "$baseDir/envs/multiqc.yaml" }
withLabel: parse { conda = "$baseDir/envs/python3.yaml" }
withLabel: prodigal { conda = "$baseDir/envs/prodigal.yaml" }
withLabel: phanotate { conda = "$baseDir/envs/phanotate.yaml" }
withLabel: python3 { conda = "$baseDir/envs/python3.yaml" }
withLabel: ratio_evalue { conda = "$baseDir/envs/python3.yaml" }
withLabel: ruby { conda = "$baseDir/envs/ruby.yaml" }
withLabel: spades { conda = "$baseDir/envs/spades.yaml" }
withLabel: virsorter { conda = "$baseDir/envs/virsorter.yaml" }
withLabel: virfinder { conda = "$baseDir/envs/virfinder.yaml" }
withLabel: checkV { conda = "$baseDir/envs/checkv.yaml" }
withNAME: ANNOTATION { conda = "$baseDir/envs/python3.yaml" }
withNAME: ASSIGN { conda = "$baseDir/envs/python3.yaml" }
withNAME: BALLOON { conda = "$baseDir/envs/balloon.yaml" }
withNAME: basics { conda = "$baseDir/envs/python3.yaml" }
withNAME: BLAST { conda = "$baseDir/envs/blast.yaml" }
withNAME: HMMSCAN { conda = "$baseDir/envs/hmmer.yaml" }
withNAME: KAIJU { conda = "$baseDir/envs/kaiju.yaml" }
withNAME: KRONA { conda = "$baseDir/envs/krona.yaml" }
withNAME: PLOT_CONTIG_MAP { conda = "$baseDir/envs/r.yaml" }
withNAME: PARSE { conda = "$baseDir/envs/python3.yaml" }
withNAME: PRODIGAL { conda = "$baseDir/envs/prodigal.yaml" }
withNAME: PHANOTATE { conda = "$baseDir/envs/phanotate.yaml" }
withNAME: python3 { conda = "$baseDir/envs/python3.yaml" }
withNAME: RATIO_EVALUE { conda = "$baseDir/envs/python3.yaml" }
withNAME: ruby { conda = "$baseDir/envs/ruby.yaml" }
withNAME: VIRSORTER { conda = "$baseDir/envs/virsorter.yaml" }
withNAME: VIRFINDER { conda = "$baseDir/envs/virfinder.yaml" }
withNAME: CHECKV { conda = "$baseDir/envs/checkv.yaml" }
}
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