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A novel method of microsatellite genotyping - by - sequencing using individual combinatorial barcoding
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egenomics/micomba
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This is an early release of the code used in the paper (***********) To reproduce the results obtained in it follow the instructions below: 1) Download all the files in the same folder 2) Download and add the read files to the code folder: IE7ZMMF01.fastq and IE7ZMMF02.fastq 3) Run extractor.py with n number of cores. Example for n=4: 3.1) python extractor.py 1 4 python extractor.py 2 4 python extractor.py 3 4 python extractor.py 4 4 4) Wait for the completion of all tasks and run "python group_and_classify.py" after modifying the line 10 with threads = n (number of cores used). 5) Look at the output in the folder "output" Some users are reporting an error related with latests update to the biopython library: We recommend to use biopython 1.66 or earlier.
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A novel method of microsatellite genotyping - by - sequencing using individual combinatorial barcoding
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