We now report an optimized protocol for the efficient generation of large quantities of high-fidelity human blastoids from naive pluripotent stem cells. Side-by-side single-cell RNA sequencing revealed similarities and differences in transcriptome profiles between pre-implantation blastoids and blastocysts, as well as post-implantation cultures, and uncovered a population resembling early migratory trophoblasts during co-culture with endometrial stromal cells. This optimized protocol will facilitate broader use of human blastoids as an accessible, perturbable, scalable, and tractable model for human blastocysts.
To reproduce results:
Leqian Yu#, Deirdre Logsdon#, Carlos A. Pinzon-Arteaga#, Jialei Duan#, Toshihiko Ezashi#, Yulei Wei#, Ana Elisa Ribeiro Orsi, Seiya Oura, Lizhong Liu, Lei Wang, Kun Liu, Xiaoyun Ding, Linfeng Zhan, Junfei Zhang, Asrafun Nahar, Caitlen Stobbe, Mandy Katz-Jaffe, William B Schoolcraft, Tao Tan, Gary C. Hon*, Ye Yuan*, Jun Wu*. (2023). Large scale production of human blastoids amenable to modeling blastocyst development and maternal-fetal crosstalk. Cell Stem Cell 30, 1246–1261.e9.
DOI: https://doi.org/10.1016/j.stem.2023.08.002