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remove HISAT2 process references and params pseudo_aligner
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maxulysse committed May 15, 2024
1 parent 759a486 commit 725e74d
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Showing 3 changed files with 3 additions and 13 deletions.
12 changes: 2 additions & 10 deletions conf/modules.config
Original file line number Diff line number Diff line change
Expand Up @@ -39,7 +39,7 @@ process {
ext.args2 = '--no-same-owner'
}

withName: 'UNTAR_.*|STAR_GENOMEGENERATE|STAR_GENOMEGENERATE_IGENOMES|HISAT2_BUILD' {
withName: 'UNTAR_.*|STAR_GENOMEGENERATE|STAR_GENOMEGENERATE_IGENOMES' {
publishDir = [
path: { "${params.outdir}/genome/index" },
mode: params.publish_dir_mode,
Expand All @@ -56,14 +56,6 @@ process {
]
}

withName: 'HISAT2_EXTRACTSPLICESITES' {
publishDir = [
path: { "${params.outdir}/genome/index" },
mode: params.publish_dir_mode,
saveAs: { filename -> filename.equals('versions.yml') ? null : params.save_reference ? filename : null }
]
}

withName: 'SALMON_INDEX' {
ext.args = { [
params.gencode ? '--gencode' : '',
Expand All @@ -84,7 +76,7 @@ process {
]
}

withName: 'CAT_ADDITIONAL_FASTA|PREPROCESS_TRANSCRIPTS_FASTA_GENCODE' {
withName: 'CUSTOM_CATADDITIONALFASTA|PREPROCESS_TRANSCRIPTS_FASTA_GENCODE' {
publishDir = [
path: { "${params.outdir}/genome" },
mode: params.publish_dir_mode,
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1 change: 0 additions & 1 deletion main.nf
Original file line number Diff line number Diff line change
Expand Up @@ -73,7 +73,6 @@ workflow NFCORE_RIBOSEQ {
params.sortmerna_index,
params.gencode,
params.aligner,
params.pseudo_aligner,
params.skip_gtf_filter,
params.skip_bbsplit,
! params.remove_ribo_rna,
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3 changes: 1 addition & 2 deletions subworkflows/local/prepare_genome/main.nf
Original file line number Diff line number Diff line change
Expand Up @@ -43,8 +43,7 @@ workflow PREPARE_GENOME {
bbsplit_index // directory: /path/to/rsem/index/
sortmerna_index // directory: /path/to/sortmerna/index/
gencode // boolean: whether the genome is from GENCODE
aligner // string: Specifies the alignment algorithm to use - available options are 'star_salmon', 'star_rsem' and 'hisat2'
pseudo_aligner // string: Specifies the pseudo aligner to use - available options are 'salmon'. Runs in addition to '--aligner'
aligner // string: Specifies the alignment algorithm to use - available options are 'star'
skip_gtf_filter // boolean: Skip filtering of GTF for valid scaffolds and/ or transcript IDs
skip_bbsplit // boolean: Skip BBSplit for removal of non-reference genome reads
skip_sortmerna // boolean: Skip sortmerna for removal of non-reference genome reads
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