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Genetic Interaction Reporting Tool to collect information of mutually exclusive and co-occurring altered gene pairs in childhood cancers

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GI_ReportingTool

Genetic Interaction Reporting Tool to collect information of mutually exclusive and co-occurring altered gene pairs in childhood cancers. For more details about the candidates we refer to the accompanying manuscript A comprehensive map of genetic interactions in childhood cancer reveals multiple underlying biological mechanisms (https://doi.org/10.1101/2020.11.17.385120)

Running the app

To aid in reproducibility and ease of deployment a Dockerfile is provided. This file is not needed when starting the Shiny server from R. To do that, the app.R file can be used. The setup.R and functions.R will be read in via the main app.R file.

Data information

www/GI_map.png

Simplified version of Figure 3 of the manuscript.

data/cand_target_dkfz.txt

Same table as table S2 of the manuscript.

data/muts_cand_target_dkfz.txt

This file contains all mutations from the TARGET and DKFZ data set, but only in genes that were part of a candidate genetic interaction in one of the two data sets. Only samples that were included in the test are considered, so hypermutators etc. are filtered out.

data/vep_out_muts_target_dkfz.txt

This file is the output file of VEP after submitting a filtered version of muts_cand_target_dkfz.txt. The VEP input file contains only columns needed for VEP and the rows where candidate=FALSE and vartype=SYN were filtered out.

Subset of the data

Unfortunately, we cannot share the complete tables, because of sensitive information. However, we will share the HGG-K27M, HGG-other, and MB-SHH information of DKFZ to get familiar with the tool. Please note, the files in the data folder only contains the information of these three cancer types and therefore only the associated candidates are shown in the reporting tool when running it from here. All candidates can be analyzed via http://gi-analysis.bioinf.prinsesmaximacentrum.nl/.

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Genetic Interaction Reporting Tool to collect information of mutually exclusive and co-occurring altered gene pairs in childhood cancers

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